##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139776_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9295249
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.499066458574696	34.0	31.0	34.0	31.0	34.0
2	32.68496884806421	34.0	31.0	34.0	31.0	34.0
3	32.779218932166316	34.0	31.0	34.0	31.0	34.0
4	36.198939910055124	37.0	37.0	37.0	35.0	37.0
5	36.043208094801976	37.0	35.0	37.0	35.0	37.0
6	36.072512312472746	37.0	35.0	37.0	35.0	37.0
7	36.08924968013229	37.0	35.0	37.0	35.0	37.0
8	36.086205974686635	37.0	35.0	37.0	35.0	37.0
9	37.96123449732223	39.0	38.0	39.0	35.0	39.0
10-11	37.844210520880075	39.0	38.0	39.0	35.0	39.0
12-13	37.86915692091734	39.0	38.0	39.0	35.0	39.0
14-15	39.18414143612506	40.0	39.0	41.0	36.0	41.0
16-17	39.15415972181057	40.0	39.0	41.0	36.0	41.0
18-19	39.18809442329086	40.0	39.0	41.0	36.0	41.0
20-21	39.22761271495892	40.0	39.0	41.0	36.0	41.0
22-23	39.189554467445404	40.0	39.0	41.0	36.0	41.0
24-25	39.17288459986714	40.0	39.0	41.0	36.0	41.0
26-27	39.06778586846056	40.0	39.0	41.0	36.0	41.0
28-29	38.98924758344083	40.0	39.0	41.0	35.0	41.0
30-31	38.867697318491444	40.0	38.0	41.0	35.0	41.0
32-33	38.806281422277266	40.0	38.0	41.0	35.0	41.0
34-35	38.7504953566127	40.0	38.0	41.0	35.0	41.0
36-37	38.67765240558019	40.0	38.0	41.0	35.0	41.0
38-39	38.59075419276783	40.0	38.0	41.0	35.0	41.0
40-41	38.57817172543877	40.0	38.0	41.0	35.0	41.0
42-43	38.52476915259051	40.0	38.0	41.0	34.5	41.0
44-45	38.492057038118034	40.0	38.0	41.0	34.0	41.0
46-47	38.385731398315684	40.0	38.0	41.0	34.0	41.0
48-49	38.36849422983683	40.0	38.0	41.0	34.0	41.0
50-51	38.28029334896664	40.0	38.0	41.0	34.0	41.0
52-53	38.10489790853014	40.0	37.0	41.0	33.5	41.0
54-55	37.95282611023429	40.0	37.0	41.0	33.0	41.0
56-57	37.74807852087481	39.0	36.5	41.0	33.0	41.0
58-59	37.51555944973891	39.0	36.0	41.0	33.0	41.0
60-61	37.340247557007054	39.0	35.5	41.0	32.5	41.0
62-63	37.16028305827966	39.0	35.0	40.0	32.0	41.0
64-65	36.88475749748418	38.0	35.0	40.0	32.0	41.0
66-67	36.67457457851178	37.5	35.0	40.0	32.0	41.0
68-69	36.38794210168476	37.0	35.0	40.0	32.0	41.0
70-71	36.044722178320086	36.5	35.0	39.0	31.0	41.0
72-73	35.788113467805616	36.0	35.0	39.0	31.0	40.5
74-75	35.44005810984435	35.5	34.0	38.5	31.0	40.0
76-77	33.45539138147031	34.5	32.0	36.0	28.0	39.0
78-79	34.6009337605776	35.0	34.0	37.0	30.5	39.0
80-81	34.58877868485179	35.0	34.0	36.5	31.0	39.0
82-83	34.364870046487354	35.0	34.0	36.0	31.0	37.5
84-85	34.11355540507207	35.0	34.0	36.0	30.5	37.0
86-87	33.924675076762455	35.0	34.0	35.0	30.0	37.0
88-89	33.73725116410898	35.0	34.0	35.0	30.0	36.0
90-91	33.70347778624191	35.0	34.0	35.0	30.0	36.0
92-93	33.63392913423134	35.0	34.0	35.0	30.0	36.0
94-95	33.56362320056677	35.0	34.0	35.0	30.0	36.0
96-97	33.57628172407077	35.0	34.0	35.0	30.0	35.0
98-99	33.64319897661045	35.0	34.0	35.0	31.0	35.0
100-101	32.39070157172897	34.0	31.5	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	4.0
19	26.0
20	54.0
21	188.0
22	741.0
23	2414.0
24	6162.0
25	13115.0
26	24290.0
27	40134.0
28	59250.0
29	83280.0
30	110827.0
31	146368.0
32	195487.0
33	272163.0
34	408040.0
35	644559.0
36	1186603.0
37	2238176.0
38	2610939.0
39	1226991.0
40	25436.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.734960623432464	19.665809920745534	12.998662004643446	24.600567451178552
2	15.44261382206798	21.24683622880841	39.74628292522128	23.564267023902328
3	18.614016009419228	25.876081277000996	30.359908544654196	25.14999416892558
4	12.951961441517156	17.475134152876453	37.60314890116694	31.96975550443945
5	13.288995978267174	37.82684849579576	35.37686518712198	13.507290338815094
6	30.876674020632777	36.94889766353878	18.340734814949332	13.833693500879107
7	26.92591666990309	32.346976396221336	22.708977457193456	18.01812947668212
8	25.448573485537334	35.65171608190167	21.176130502867917	17.723579929693077
9	26.15452143925665	15.856749951453516	20.572100806705386	37.41662780258444
10-11	24.822202559740923	26.14223892044909	28.5775284949536	20.45803002485638
12-13	25.4657291899705	23.738215131827484	28.633368137902476	22.162687540299544
14-15	22.572250128372115	25.04942581920666	26.024429915387902	26.35389413703332
16-17	22.414461041166476	28.247831759350433	26.690018481564	22.64768871791909
18-19	22.54301095107834	27.35848173620739	28.22669946765278	21.871807845061493
20-21	23.1212089555338	26.61447027128275	28.080962115564596	22.18335865761885
22-23	22.860999780177522	26.74465397219649	27.945102052827764	22.449244194798222
24-25	22.758781062814453	26.86429626885387	28.057171201522706	22.31975146680897
26-27	22.669032749684686	27.030804165259525	28.02674405381376	22.27341903124203
28-29	22.581286657967873	27.00039858998926	27.96970285057035	22.44861190147252
30-31	22.709571627998262	27.00245424974495	28.0305502934922	22.257423828764583
32-33	22.611791392096286	27.132914608704713	27.90818969053027	22.34710430866873
34-35	22.82768421173127	26.98461134250515	27.98650664774087	22.20119779802271
36-37	22.547447898948757	27.181023004677378	27.870514598214776	22.401014498159093
38-39	22.409850180911885	26.975016083679005	28.03668605963609	22.578447675773024
40-41	22.591756813425672	26.85538200780892	28.0071059202934	22.54575525847201
42-43	22.647955548592	26.88659368234938	27.828696386069794	22.636754382988823
44-45	22.478475392183856	27.088947646102095	27.745788045207565	22.68678891650648
46-47	22.84032836206589	26.884220274562697	27.315398651449428	22.96005271192198
48-49	22.577306437258972	27.065387125482054	27.34736873331356	23.009937703945415
50-51	22.54596122199381	27.221092056732193	27.41250088442502	22.820445836848975
52-53	22.743679252606988	27.091784061275582	27.37205887855217	22.792477807565263
54-55	22.59704776227374	27.04441793181725	27.24496740084051	23.1135669050685
56-57	22.80388451566347	27.212347139923498	27.03187589471881	22.95189244969422
58-59	22.571210767810676	27.36254599361464	27.304571662052844	22.761671576521834
60-61	22.837733383619376	27.42434978484432	27.004648454586178	22.733268376950125
62-63	22.673701116499075	27.588212520923495	26.994926619626607	22.74315974295082
64-65	22.59943413570397	27.534119653902223	26.973680545330826	22.892765665062985
66-67	22.735834627198614	27.48621496730413	26.793779928463906	22.98417047703335
68-69	22.76822730367483	27.30625181879977	26.94264264564757	22.98287823187783
70-71	22.7327239488117	27.125320797423175	26.995163227996866	23.14679202576826
72-73	22.776996903523425	27.086366798224553	26.896324075206145	23.240312223045876
74-75	22.668196453836988	27.18422376840316	26.911439790177656	23.2361399875822
76-77	22.96705887392664	27.094786149104422	26.796030028895007	23.14212494807393
78-79	22.9195950131494	27.072995572033086	26.716847973530594	23.29056144128692
80-81	22.94312789865115	27.094597145108253	26.692916038354568	23.26935891788603
82-83	22.966831655055877	27.077294924140272	26.795621597788006	23.160251823015845
84-85	23.032972493482948	27.076591606542866	26.73718863247714	23.153247267497047
86-87	22.888055476306494	27.065149881715516	26.88405438056549	23.162740261412495
88-89	22.957661635931608	27.054207898384586	26.8319289991583	23.156201466525506
90-91	23.06535912680191	26.97729154199653	26.88580504036126	23.071544290840304
92-93	23.10701882297847	26.8936140165291	26.961302863332314	23.038064297160116
94-95	23.07560617881794	26.91626728545585	26.98148347806893	23.02664305765728
96-97	23.001017705717164	26.90891907285429	27.054661687974104	23.035401533454447
98-99	23.259004781646837	27.53092320332596	27.057158752416953	22.15291326261025
100-101	27.660023909421582	34.469728265926825	27.632475324516758	10.237772500134838
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1907.0
1	1756.0
2	2508.5
3	3703.0
4	4958.5
5	7743.5
6	10209.5
7	12070.5
8	13603.5
9	13767.0
10	12779.0
11	11693.5
12	12367.5
13	15674.5
14	23364.5
15	33459.5
16	41476.0
17	46408.0
18	48082.0
19	46672.5
20	43876.0
21	41529.0
22	41303.0
23	44931.5
24	52637.0
25	64394.5
26	80195.5
27	97558.5
28	115666.5
29	135430.0
30	154296.0
31	172512.5
32	191449.5
33	210099.5
34	227787.5
35	244448.5
36	264102.5
37	282121.5
38	296880.0
39	311515.5
40	325927.0
41	339263.5
42	348607.0
43	358230.5
44	371774.0
45	387253.0
46	404667.5
47	420101.5
48	425095.5
49	424436.0
50	424033.5
51	419239.5
52	407877.5
53	393054.5
54	377658.5
55	358475.0
56	336548.0
57	311281.0
58	281527.0
59	249238.0
60	214627.0
61	180395.0
62	146962.5
63	116481.5
64	90416.0
65	69894.0
66	54358.5
67	42456.5
68	32812.0
69	23900.0
70	16896.5
71	12196.5
72	8656.5
73	6045.0
74	4214.0
75	2934.0
76	2005.0
77	1350.5
78	931.5
79	637.0
80	433.0
81	289.0
82	202.5
83	144.0
84	93.5
85	63.0
86	47.0
87	38.0
88	32.5
89	25.0
90	18.0
91	12.0
92	7.0
93	3.0
94	1.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.001839649481148918
3	0.0023775586861632214
4	7.53072887020025E-4
5	2.1516368200572142E-5
6	4.0881099581087073E-4
7	0.0
8	5.3790920501430354E-5
9	3.657782594097264E-4
10-11	5.432882970644466E-4
12-13	1.4523548535386196E-4
14-15	6.454910460171643E-5
16-17	4.3032736401144283E-4
18-19	0.0
20-21	5.9173045924469906E-5
22-23	1.6139680108312318E-5
24-25	0.0
26-27	7.53826287624143E-5
28-29	2.3167518019075918E-4
30-31	5.993182026544289E-4
32-33	3.469871888534703E-4
34-35	0.0010247734623792036
36-37	1.2633970902097382E-4
38-39	1.221719484654287E-4
40-41	3.1453955733724744E-4
42-43	0.0014093606895547223
44-45	4.4921019345467347E-4
46-47	1.227760650516703E-4
48-49	4.093723971109225E-4
50-51	0.0
52-53	5.789005849150573E-4
54-55	7.588100624383467E-4
56-57	8.19236933952618E-4
58-59	7.739154954058283E-4
60-61	7.081886697023496E-4
62-63	1.1759347309186951E-4
64-65	7.494395949365208E-4
66-67	0.002662596332061219
68-69	0.00146666959086772
70-71	2.716106490596108E-4
72-73	0.0
74-75	9.32912871894055E-5
76-77	2.185593456741168E-5
78-79	0.0011245315900528965
80-81	1.7294688177148137E-4
82-83	9.935826787661598E-5
84-85	8.462874843242713E-4
86-87	2.68253845773921E-4
88-89	8.025377527803317E-6
90-91	4.085311434320979E-5
92-93	1.41556005259555E-4
94-95	7.391566986713022E-4
96-97	2.6054927086589296E-5
98-99	9.072644208545614E-6
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	347.0
20-21	744.0
22-23	1653.0
24-25	5191.0
26-27	5478.0
28-29	13179.0
30-31	36033.0
32-33	45937.0
34-35	62206.0
36-37	93331.0
38-39	103804.0
40-41	102765.0
42-43	111462.0
44-45	128332.0
46-47	126943.0
48-49	119625.0
50-51	109104.0
52-53	98058.0
54-55	107584.0
56-57	112371.0
58-59	115312.0
60-61	115425.0
62-63	112858.0
64-65	117238.0
66-67	124932.0
68-69	117591.0
70-71	110993.0
72-73	103487.0
74-75	104956.0
76-77	103031.0
78-79	108277.0
80-81	108915.0
82-83	102686.0
84-85	102078.0
86-87	105995.0
88-89	109545.0
90-91	114464.0
92-93	116997.0
94-95	124072.0
96-97	174477.0
98-99	702679.0
100-101	4915094.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.27450429196708
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.21262359397556	76.9283675785408
2	6.802586417618659	11.601743693313985
3	1.6334911463409496	4.178854433086245
4	0.6033730787943808	2.0580936078923524
5	0.28661627217930324	1.22205302660508
6	0.14931845956755269	0.7639834572757909
7	0.08633248797332856	0.5153372081352462
8	0.05670303164818244	0.3868258332520383
9	0.0393237409489769	0.30179812646967585
>10	0.12530489712202073	1.715294270542363
>50	0.0033490213703379497	0.18618024984272416
>100	9.353937294257206E-4	0.11873097203612208
>500	4.245873124288301E-5	0.022737543007609946
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0758184100286071E-5
2	0.0	4.3032736401144284E-5	0.0	0.0	1.0758184100286071E-5
3	0.0	4.3032736401144284E-5	0.0	0.0	1.0758184100286071E-5
4	0.0	1.0758184100286071E-4	0.0	1.0758184100286071E-5	2.1516368200572142E-5
5	0.0	1.1834002510314678E-4	0.0	1.0758184100286071E-5	2.1516368200572142E-5
6	5.3790920501430354E-5	1.1834002510314678E-4	0.0	1.0758184100286071E-5	4.3032736401144284E-5
7	5.3790920501430354E-5	1.1834002510314678E-4	0.0	1.0758184100286071E-5	4.3032736401144284E-5
8	5.3790920501430354E-5	1.5061457740400498E-4	0.0	1.0758184100286071E-5	4.3032736401144284E-5
9	5.3790920501430354E-5	3.550200753094403E-4	0.0	2.1516368200572142E-5	4.3032736401144284E-5
10-11	5.3790920501430354E-5	3.550200753094403E-4	0.0	4.3032736401144284E-5	4.3032736401144284E-5
12-13	5.3790920501430354E-5	3.550200753094403E-4	0.0	7.530728870200249E-5	4.3032736401144284E-5
14-15	5.3790920501430354E-5	4.7873919246273016E-4	0.0	1.0758184100286071E-4	5.3790920501430354E-5
16-17	1.2909820920343286E-4	4.841182845128732E-4	0.0	1.8826822175500624E-4	6.454910460171643E-5
18-19	1.2909820920343286E-4	5.002555606633023E-4	0.0	3.3350370710886817E-4	7.530728870200249E-5
20-21	1.2909820920343286E-4	5.325301129641605E-4	0.0	0.001237191171532898	8.068638075214553E-5
22-23	1.2909820920343286E-4	5.540464811647326E-4	0.0	0.0026626505648208025	9.14445648524316E-5
24-25	1.2909820920343286E-4	5.863210334655909E-4	0.0	0.005518948443446754	1.0220274895271767E-4
26-27	1.2909820920343286E-4	6.13216493716306E-4	0.0	0.012732310882688565	1.0758184100286071E-4
28-29	1.2909820920343286E-4	6.13216493716306E-4	0.0	0.05678169568130988	1.0758184100286071E-4
30-31	1.2909820920343286E-4	6.777655983180225E-4	0.0	0.08268202390274859	1.0758184100286071E-4
32-33	1.2909820920343286E-4	6.885237824183085E-4	0.0	0.09285926606161922	1.0758184100286071E-4
34-35	1.2909820920343286E-4	7.853474393208831E-4	0.0	0.10631237527902695	1.1834002510314678E-4
36-37	1.2909820920343286E-4	9.19824740574459E-4	0.0	0.12610205493150317	1.1834002510314678E-4
38-39	1.2909820920343286E-4	9.897529372263185E-4	0.0	0.15962993568004472	1.2909820920343286E-4
40-41	1.2909820920343286E-4	0.0011134720543796084	0.0	0.23027893066662336	1.5061457740400498E-4
42-43	1.2909820920343286E-4	0.0011780211589813246	0.0	0.2589548703859359	1.5061457740400498E-4
44-45	1.2909820920343286E-4	0.0013178775522850435	0.0	0.29604908916372225	1.5061457740400498E-4
46-47	1.2909820920343286E-4	0.0014308384853380473	0.0	0.336091050384987	1.6137276150429106E-4
48-49	1.2909820920343286E-4	0.0014415966694383336	0.0	0.36919936195361736	1.828891297048632E-4
50-51	1.7213094560457714E-4	0.0015653157865916233	0.0	0.40037120038419627	1.828891297048632E-4
52-53	1.828891297048632E-4	0.0015706948786417664	0.0	0.4440117741870067	2.0440549790543537E-4
54-55	1.828891297048632E-4	0.0015814530627420525	0.0	0.5301686915541477	2.259218661060075E-4
56-57	1.828891297048632E-4	0.0015922112468423385	0.0	0.6230925067203686	2.3130095815615054E-4
58-59	1.828891297048632E-4	0.0016083485229927675	0.0	0.7105726807318449	2.3668005020629357E-4
60-61	2.0440549790543534E-4	0.0016513812593939117	0.0	0.7623840953588226	2.3668005020629357E-4
62-63	2.259218661060075E-4	0.0016728976275944842	0.0	0.8057395772829754	2.474382343065796E-4
64-65	2.259218661060075E-4	0.001839649481148918	0.0	0.8238187056635062	2.474382343065796E-4
66-67	2.3130095815615054E-4	0.0018611658493494902	0.0	0.8334257640650616	2.474382343065796E-4
68-69	2.474382343065796E-4	0.0018826822175500625	0.0	0.8427692469561601	2.474382343065796E-4
70-71	2.474382343065796E-4	0.0019095776778007777	0.0	0.8505743095209176	2.474382343065796E-4
72-73	2.6895460250715177E-4	0.0019310940460013498	0.0	0.8570238408890392	2.6895460250715177E-4
74-75	2.6895460250715177E-4	0.001957989506252065	0.0	0.8623706583868813	2.6895460250715177E-4
76-77	2.743336945572948E-4	0.0020064013347033524	0.0	0.8668137884202994	2.743336945572948E-4
78-79	2.797127866074378E-4	0.0020117804267534953	0.0	0.8707190092487033	2.8509187865758084E-4
80-81	2.904709707077239E-4	0.002017159518803638	0.0	0.8727038942152061	2.9585006275786695E-4
82-83	2.904709707077239E-4	0.002022538610853781	0.0	0.873338627077123	3.0122915480800997E-4
84-85	2.904709707077239E-4	0.0020386758870042107	0.0	0.8739034317423879	3.0122915480800997E-4
86-87	2.904709707077239E-4	0.0020548131631546395	0.0	0.8742369354494968	3.0122915480800997E-4
88-89	3.0122915480800997E-4	0.0020978458995557837	0.0	0.8743391381984496	3.119873389082961E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10500	0.0	48.975307	1
GTATCAA	18790	0.0	34.66014	1
TCAACGC	22660	0.0	28.028988	4
ATCAACG	23065	0.0	27.53638	3
CAACGCA	23380	0.0	27.113962	5
TATCAAC	24015	0.0	26.632387	2
AACGCAG	24140	0.0	26.343805	6
GTACATG	31125	0.0	22.594452	1
TACATGG	30770	0.0	22.593182	2
ACGCAGA	28605	0.0	22.259817	7
CGCAGAG	29085	0.0	21.864744	8
ACATGGG	31300	0.0	21.540497	3
CATGGGG	23620	0.0	19.280115	4
GTGGTAT	5135	0.0	19.149841	1
ACCGAAC	1580	0.0	18.61968	8
GCAGAGT	34390	0.0	18.421675	9
CTTATAC	15145	0.0	18.174698	1
GAGTACT	18020	0.0	17.74554	12-13
TATACAC	17025	0.0	17.516554	3
GGACCGA	2105	0.0	17.230553	6
>>END_MODULE