FastQCFastQC Report
Fri 27 May 2016
SRR2139775_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139775_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10036875
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94900.045.873851
GTATCAA167700.034.925691
TCAACGC192450.029.636684
ATCAACG196700.029.0178283
CAACGCA199300.028.6386995
AACGCAG206800.027.6602866
TATCAAC211250.027.2746452
ACGCAGA248500.023.1020417
CGCAGAG253900.022.6433288
GTACATG266350.021.2279781
TACATGG263750.020.7933372
ACATGGG261550.020.2868423
GCAGAGT311600.018.3307539
GAGTACT167850.018.03865412-13
CATGGGG189150.017.7374924
AGAGTAC269300.017.04946110-11
CAGAGTA302950.016.46140310-11
TAGACCG6600.016.3170175
GTACTTT195900.015.403501514-15
TATGCCG59700.015.38713842-43
Produced by FastQC (version 0.11.5)