##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139775_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10036875
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.515042181954044	34.0	31.0	34.0	31.0	34.0
2	32.7052532785354	34.0	31.0	34.0	31.0	34.0
3	32.79885253129086	34.0	31.0	34.0	31.0	34.0
4	36.2119873964755	37.0	37.0	37.0	35.0	37.0
5	36.05980636403263	37.0	35.0	37.0	35.0	37.0
6	36.08471481412292	37.0	36.0	37.0	35.0	37.0
7	36.10435100566661	37.0	35.0	37.0	35.0	37.0
8	36.0997535587521	37.0	35.0	37.0	35.0	37.0
9	37.971432841397345	39.0	38.0	39.0	35.0	39.0
10-11	37.85536020922847	39.0	38.0	39.0	35.0	39.0
12-13	37.88695380783361	39.0	38.0	39.0	35.0	39.0
14-15	39.225367955663486	40.0	39.0	41.0	36.0	41.0
16-17	39.19554233762999	40.0	39.0	41.0	36.0	41.0
18-19	39.22499483155863	40.0	39.0	41.0	36.0	41.0
20-21	39.26511087765421	40.0	39.0	41.0	36.0	41.0
22-23	39.22914862363321	40.0	39.0	41.0	36.0	41.0
24-25	39.220528297537754	40.0	39.0	41.0	36.0	41.0
26-27	39.12384205034461	40.0	39.0	41.0	36.0	41.0
28-29	39.04818555805456	40.0	39.0	41.0	36.0	41.0
30-31	38.938809283508874	40.0	38.5	41.0	35.0	41.0
32-33	38.88304863517963	40.0	38.0	41.0	35.0	41.0
34-35	38.82751835454316	40.0	38.0	41.0	35.0	41.0
36-37	38.763473253732776	40.0	38.0	41.0	35.0	41.0
38-39	38.68231461811284	40.0	38.0	41.0	35.0	41.0
40-41	38.67180989025927	40.0	38.0	41.0	35.0	41.0
42-43	38.620949289732096	40.0	38.0	41.0	35.0	41.0
44-45	38.59746423492646	40.0	38.0	41.0	35.0	41.0
46-47	38.50052595182444	40.0	38.0	41.0	34.0	41.0
48-49	38.49079823740612	40.0	38.0	41.0	34.0	41.0
50-51	38.426070163587724	40.0	38.0	41.0	34.0	41.0
52-53	38.26223337070165	40.0	38.0	41.0	34.0	41.0
54-55	38.126901159204365	40.0	37.0	41.0	33.5	41.0
56-57	37.9421855910433	40.0	37.0	41.0	33.0	41.0
58-59	37.73184195494544	39.5	36.5	41.0	33.0	41.0
60-61	37.57873901322036	39.0	36.0	41.0	33.0	41.0
62-63	37.41621532531541	39.0	36.0	41.0	33.0	41.0
64-65	37.157832000340406	39.0	35.0	40.0	32.0	41.0
66-67	36.96151236255389	38.0	35.0	40.0	32.0	41.0
68-69	36.683526599345484	37.5	35.0	40.0	32.0	41.0
70-71	36.35267989398247	37.0	35.0	39.5	32.0	41.0
72-73	36.093670347794216	36.5	35.0	39.0	32.0	41.0
74-75	35.73442651917742	36.0	34.5	39.0	31.0	40.5
76-77	33.716028978918814	34.5	32.0	36.5	28.5	39.0
78-79	34.84895666372587	35.0	34.0	37.0	30.5	39.0
80-81	34.8131262266166	35.0	34.0	37.0	31.0	39.0
82-83	34.563321507027894	35.0	34.0	36.5	31.0	39.0
84-85	34.28364706050006	35.0	34.0	36.0	31.0	37.0
86-87	34.06227271724579	35.0	34.0	36.0	30.5	37.0
88-89	33.8499447369295	35.0	34.0	35.0	30.0	37.0
90-91	33.79205204651473	35.0	34.0	35.0	30.5	36.0
92-93	33.70709802984531	35.0	34.0	35.0	30.0	36.0
94-95	33.622702046006054	35.0	34.0	35.0	30.0	36.0
96-97	33.62409848443035	35.0	34.0	35.0	30.5	36.0
98-99	33.681914885580525	35.0	34.0	35.0	31.0	35.0
100-101	32.47039369147667	34.0	31.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	8.0
19	18.0
20	49.0
21	167.0
22	724.0
23	2334.0
24	6191.0
25	13550.0
26	25086.0
27	41808.0
28	62304.0
29	87457.0
30	116991.0
31	154650.0
32	205918.0
33	282466.0
34	417628.0
35	656902.0
36	1204902.0
37	2356265.0
38	2992937.0
39	1379985.0
40	28533.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.17177159225356	20.086779998754594	13.15089607073915	24.590552338252696
2	15.094455612378285	21.46792283338541	40.25874313716738	23.178878417068923
3	18.36381762668876	25.856630961504663	30.592237803791782	25.18731360801479
4	12.585065496309838	17.78475763002742	38.65982519372411	30.970351679938624
5	13.021453390622082	37.921076032131516	35.999750918488076	13.05771965875833
6	30.304009121598767	37.916516926597495	18.61530679436161	13.16416715744213
7	26.59016875272433	32.5003947941964	23.207631857525378	17.701804595553895
8	24.65260584225959	36.56996653339139	21.460216182933525	17.317211441415502
9	25.643901205032225	16.279457641344223	21.12391695104439	36.95272420257916
10-11	24.186626538440866	26.649367372183562	29.28178835031056	19.882217739065005
12-13	24.808348906764557	24.184612935182212	29.29158414998158	21.71545400807165
14-15	21.904937590351253	25.69496395265476	26.537891122326542	25.86220733466745
16-17	21.80216806555657	28.856329353074962	27.317102939150846	22.024399642217624
18-19	21.850142599165576	28.13874338377234	28.71947194719472	21.291642069867365
20-21	22.44468491383281	27.434053849743478	28.61301367389139	21.508247562532322
22-23	22.150481628282403	27.447772429646317	28.48004976789878	21.9216961741725
24-25	22.024856525758732	27.558392023999843	28.54699965319095	21.869751797050473
26-27	21.972258777478658	27.761738890685145	28.47040370381542	21.795598628020773
28-29	21.885928543820857	27.80513449889831	28.508962285381024	21.79997467189981
30-31	22.195176522304113	27.649304177096855	28.394274218437033	21.761245082162
32-33	21.918405043296644	27.784958911097835	28.430303202608293	21.866332842997227
34-35	22.016449032339665	27.72720136545236	28.443476755578367	21.812872846629606
36-37	21.99994792240276	27.810911107695556	28.356353015997044	21.83278795390464
38-39	21.840044579840825	27.74030771167683	28.532605446641995	21.887042261840346
40-41	22.050180343001994	27.61475701521055	28.44174129987489	21.893321341912568
42-43	22.059104758735955	27.644058033941544	28.311411623575445	21.98542558374706
44-45	21.95910352557918	27.840025931947785	28.19586427095645	22.005006271516585
46-47	22.157671376025412	27.70997977138146	27.895611167390488	22.23673768520264
48-49	22.008878636741393	27.818439868958748	27.93837554526387	22.23430594903599
50-51	21.95160458741183	27.943795948368656	27.957844343563103	22.146755120656408
52-53	22.095189442878922	27.885872744401247	27.887702325027753	22.131235487692074
54-55	22.081639055924487	27.765676967064284	27.84547588825635	22.307208088754884
56-57	22.131149893979938	27.954719292949665	27.713347822075868	22.200782990994526
58-59	21.996303931817675	27.968168778799186	27.926556547730403	22.10897074165274
60-61	22.153193965692648	27.987581254271877	27.742079138695207	22.11714564134027
62-63	22.024403892506847	28.17577653999443	27.699317590838735	22.100501976659988
64-65	22.005383723155205	28.116523707044195	27.670163685027394	22.207928884773207
66-67	22.059841888124552	28.15207830473423	27.539162093852553	22.24891771328867
68-69	22.059655987688185	28.07820260121079	27.593582660536654	22.26855875056437
70-71	22.072559908360503	27.917185887015766	27.62200996435572	22.38824424026801
72-73	22.06423861981557	27.925012571432845	27.56554125081239	22.445207557939195
74-75	22.01506946887846	28.024633523460103	27.53092693993162	22.429370067729824
76-77	22.207151471486522	27.95960240408911	27.43697544800181	22.39627067642256
78-79	22.199649837596734	27.91532615465424	27.376543005931612	22.508481001817408
80-81	22.148603258960605	27.998153299889267	27.387130210816924	22.466113230333207
82-83	22.207344602469654	27.96253851024758	27.435977806372357	22.394139080910406
84-85	22.286220081366647	27.98147353146017	27.394295987644877	22.3380103995283
86-87	22.177188119533223	28.015437433550982	27.463474479776114	22.343899967139684
88-89	22.221433150668673	28.02456029219304	27.45699857286141	22.297007984276878
90-91	22.215821027972513	28.092667837795855	27.49129434053267	22.200216793698964
92-93	22.28869535974331	28.007528478564126	27.489993998622968	22.213782163069595
94-95	22.201461806685987	28.021094761858155	27.54168096165047	22.23576246980538
96-97	22.155908865548714	28.031593745013872	27.620038786352154	22.192458603085264
98-99	22.38038211597283	28.54413457336954	27.726326916614212	21.349156394043415
100-101	26.40128139666529	35.581679741879654	28.36315122363875	9.653887637816316
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2824.0
1	2364.5
2	2632.5
3	3580.5
4	4704.5
5	7276.5
6	9583.5
7	11230.5
8	12345.5
9	12241.5
10	11234.0
11	10362.5
12	11259.5
13	14534.0
14	21856.5
15	31485.0
16	39235.0
17	44097.0
18	45982.5
19	45394.5
20	43947.0
21	43034.0
22	44368.5
23	49924.0
24	60095.0
25	74796.0
26	93476.5
27	114352.0
28	137443.5
29	161202.0
30	183456.5
31	205849.0
32	229082.5
33	252340.0
34	273743.0
35	292628.5
36	314329.5
37	334245.0
38	350578.0
39	366214.5
40	381994.0
41	396323.0
42	405312.5
43	414202.0
44	423378.5
45	431118.0
46	437557.5
47	442472.5
48	441568.0
49	435771.5
50	427618.0
51	413593.0
52	393737.0
53	371406.0
54	349409.5
55	325974.5
56	300703.0
57	273438.5
58	244481.5
59	213744.0
60	180903.0
61	148868.0
62	119814.0
63	94469.0
64	72249.0
65	54584.5
66	41547.5
67	31974.0
68	24743.5
69	18314.5
70	13367.5
71	9912.0
72	7052.5
73	5010.0
74	3566.0
75	2453.5
76	1670.0
77	1109.0
78	753.5
79	523.0
80	354.5
81	231.5
82	167.5
83	122.0
84	90.0
85	66.0
86	49.0
87	33.5
88	21.5
89	15.0
90	10.5
91	7.0
92	5.0
93	3.5
94	2.5
95	3.0
96	3.0
97	2.5
98	1.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0020225418768291927
3	0.0023015131701849432
4	4.981630238495547E-4
5	0.0
6	3.7860389812566166E-4
7	0.0
8	4.981630238495547E-5
9	4.7823650289557256E-4
10-11	4.931813936110592E-4
12-13	1.5941216763185753E-4
14-15	5.4797932623451025E-5
16-17	4.7325487265707707E-4
18-19	0.0
20-21	6.974614843086361E-5
22-23	1.9929476557778816E-5
24-25	0.0
26-27	9.472919764269882E-5
28-29	2.5946765022203945E-4
30-31	6.448270896159194E-4
32-33	4.0614647025623936E-4
34-35	0.0010473004170168997
36-37	1.4691129389690465E-4
38-39	1.6350547564509296E-4
40-41	2.732188886424764E-4
42-43	0.001332129213411523
44-45	4.050535320326065E-4
46-47	1.651095680408807E-4
48-49	3.935837226712042E-4
50-51	0.0
52-53	4.904574612466966E-4
54-55	7.515014581633293E-4
56-57	6.979584321849797E-4
58-59	7.004610058483369E-4
60-61	7.781397376424061E-4
62-63	8.750921982555212E-5
64-65	8.268045304163669E-4
66-67	0.002537866129836154
68-69	0.001456423799906789
70-71	2.8295865506552584E-4
72-73	0.0
74-75	1.1989128258495197E-4
76-77	6.392833480240647E-6
78-79	0.0010559437030416815
80-81	2.4305541413817872E-4
82-83	1.4656273414645894E-4
84-85	9.049210891927368E-4
86-87	2.533504223899326E-4
88-89	2.0846308712846753E-5
90-91	4.9402797807475607E-5
92-93	1.6499096817919627E-4
94-95	7.29796016904703E-4
96-97	2.9781810267978962E-5
98-99	7.761220765518697E-6
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	345.0
20-21	848.0
22-23	1738.0
24-25	4026.0
26-27	6487.0
28-29	14066.0
30-31	28985.0
32-33	38198.0
34-35	53268.0
36-37	80071.0
38-39	87746.0
40-41	88998.0
42-43	99052.0
44-45	115682.0
46-47	116029.0
48-49	108091.0
50-51	98359.0
52-53	88871.0
54-55	97632.0
56-57	101944.0
58-59	105267.0
60-61	105838.0
62-63	102598.0
64-65	109364.0
66-67	115975.0
68-69	112000.0
70-71	106284.0
72-73	100032.0
74-75	103004.0
76-77	101183.0
78-79	106622.0
80-81	107285.0
82-83	101971.0
84-85	100998.0
86-87	105201.0
88-89	110048.0
90-91	114031.0
92-93	116930.0
94-95	127899.0
96-97	192430.0
98-99	800365.0
100-101	5761114.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.53760825749495
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.56328585881886	78.37130154162
2	6.725988667675741	11.641019449353477
3	1.5620870303553485	4.055378264910143
4	0.5425069200786624	1.877890053069896
5	0.2351418269559931	1.0174305653034699
6	0.12639251600971813	0.6562623622276889
7	0.07271821725632142	0.4405002418677668
8	0.04456065851290463	0.3084938248068599
9	0.03000051655341852	0.23365556541200028
>10	0.09555590834691942	1.2386125795167033
>50	0.0014525196220616	0.08221164926791806
>100	2.740822150484548E-4	0.04967676613642622
>500	3.527759902904541E-5	0.02756713650760057
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.992652095398219E-5
2	9.963260476991096E-6	1.992652095398219E-5	0.0	0.0	1.992652095398219E-5
3	9.963260476991096E-6	1.992652095398219E-5	0.0	0.0	1.992652095398219E-5
4	9.963260476991096E-6	3.985304190796438E-5	0.0	3.985304190796438E-5	1.992652095398219E-5
5	9.963260476991096E-6	3.985304190796438E-5	0.0	3.985304190796438E-5	1.992652095398219E-5
6	7.970608381592877E-5	3.985304190796438E-5	0.0	3.985304190796438E-5	1.992652095398219E-5
7	7.970608381592877E-5	3.985304190796438E-5	0.0	3.985304190796438E-5	1.992652095398219E-5
8	7.970608381592877E-5	3.985304190796438E-5	0.0	3.985304190796438E-5	1.992652095398219E-5
9	7.970608381592877E-5	8.966934429291986E-5	0.0	6.974282333893766E-5	1.992652095398219E-5
10-11	8.966934429291986E-5	8.966934429291986E-5	0.0	9.465097453141541E-5	2.9889781430973285E-5
12-13	8.966934429291986E-5	1.2454075596238869E-4	0.0	9.963260476991096E-5	3.487141166946883E-5
14-15	9.465097453141541E-5	1.9428357930132637E-4	0.0	1.0959586524690205E-4	3.985304190796438E-5
16-17	1.6439379787035308E-4	1.9926520953982192E-4	0.0	1.5443053739336198E-4	3.985304190796438E-5
18-19	1.793386885858397E-4	2.191917304938041E-4	0.0	1.793386885858397E-4	4.981630238495548E-5
20-21	1.793386885858397E-4	2.3911825144778628E-4	0.0	2.291549909707952E-4	6.974282333893766E-5
22-23	2.0424683977831746E-4	2.4409988168628183E-4	0.0	3.686406376486705E-4	6.974282333893766E-5
24-25	2.540631421632729E-4	2.5904477240176846E-4	0.0	4.7823650289557256E-4	7.970608381592877E-5
26-27	2.7897129335575064E-4	2.5904477240176846E-4	0.0	7.073914938663678E-4	7.970608381592877E-5
28-29	2.7897129335575064E-4	2.5904477240176846E-4	0.0	0.0018182950370508751	7.970608381592877E-5
30-31	2.839529235942462E-4	2.988978143097329E-4	0.0	0.005748801295223862	7.970608381592877E-5
32-33	3.0886107478672397E-4	2.988978143097329E-4	0.0	0.012797808082695062	7.970608381592877E-5
34-35	3.337692259792017E-4	3.7860389812566166E-4	0.0	0.022576748240861824	8.966934429291986E-5
36-37	3.3875085621769724E-4	4.6329161218008593E-4	0.0	0.03637088237125599	1.1955912572389314E-4
38-39	3.3875085621769724E-4	5.180895448035369E-4	0.0	0.06080079706083816	1.2952238620088423E-4
40-41	3.3875085621769724E-4	6.077588890964568E-4	0.0	0.1186076343483405	1.3450401643937978E-4
42-43	3.3875085621769724E-4	6.326670402889346E-4	0.0	0.14025281773460366	1.3948564667787532E-4
44-45	3.4871411669468833E-4	6.924466031508811E-4	0.0	0.16993337069556014	1.3948564667787532E-4
46-47	3.686406376486705E-4	7.322996450588455E-4	0.0	0.20311102808394046	1.3948564667787532E-4
48-49	3.786038981256616E-4	7.37281275297341E-4	0.0	0.2303754903792266	1.4446727691637087E-4
50-51	3.8358552836415715E-4	7.721526869668098E-4	0.0	0.2567034061896756	1.4944890715486644E-4
52-53	3.9853041907964384E-4	7.771343172053054E-4	0.0	0.2972937293729373	1.5941216763185753E-4
54-55	3.9853041907964384E-4	7.870975776822966E-4	0.0	0.3852145214521452	1.5941216763185753E-4
56-57	3.9853041907964384E-4	8.169873591132699E-4	0.0	0.4801245407559624	1.6937542810884862E-4
58-59	4.035120493181394E-4	8.468771405442431E-4	0.0	0.5660825705212031	1.793386885858397E-4
60-61	4.4336509122610375E-4	8.767669219752164E-4	0.0	0.6138414596176599	1.793386885858397E-4
62-63	4.8819976337256365E-4	8.966934429291986E-4	0.0	0.6394968553459119	1.793386885858397E-4
64-65	5.081262843265458E-4	9.963260476991095E-4	0.0	0.655159100815742	1.793386885858397E-4
66-67	5.081262843265458E-4	0.0010311974593685783	0.0	0.6627660501899246	1.793386885858397E-4
68-69	5.131079145650414E-4	0.0010660688710380471	0.0	0.6706021545550782	1.8432031882433526E-4
70-71	5.28052805280528E-4	0.0010760321315150382	0.0	0.6776661062332648	1.893019490628308E-4
72-73	5.679058471884925E-4	0.0010909770222305248	0.0	0.6828320567905848	1.893019490628308E-4
74-75	5.679058471884925E-4	0.0011258484338999937	0.0	0.6872457811818917	1.9428357930132637E-4
76-77	5.72887477426988E-4	0.0011756647362849493	0.0	0.6911862507005417	1.9926520953982192E-4
78-79	5.778691076654836E-4	0.0011856279967619404	0.0	0.694807895883928	1.9926520953982192E-4
80-81	5.778691076654836E-4	0.001190609627000436	0.0	0.6964518338626315	2.1421010025530856E-4
82-83	5.778691076654836E-4	0.0012055545177159226	0.0	0.6970944641633975	2.191917304938041E-4
84-85	5.928139983809702E-4	0.0012155177781929137	0.0	0.6974929945824772	2.191917304938041E-4
86-87	5.977956286194658E-4	0.0012354442991468959	0.0	0.697692259792017	2.191917304938041E-4
88-89	6.077588890964568E-4	0.0013151503829628246	0.0	0.6977968740270254	2.2417336073229965E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9490	0.0	45.87385	1
GTATCAA	16770	0.0	34.92569	1
TCAACGC	19245	0.0	29.63668	4
ATCAACG	19670	0.0	29.017828	3
CAACGCA	19930	0.0	28.638699	5
AACGCAG	20680	0.0	27.660286	6
TATCAAC	21125	0.0	27.274645	2
ACGCAGA	24850	0.0	23.102041	7
CGCAGAG	25390	0.0	22.643328	8
GTACATG	26635	0.0	21.227978	1
TACATGG	26375	0.0	20.793337	2
ACATGGG	26155	0.0	20.286842	3
GCAGAGT	31160	0.0	18.330753	9
GAGTACT	16785	0.0	18.038654	12-13
CATGGGG	18915	0.0	17.737492	4
AGAGTAC	26930	0.0	17.049461	10-11
CAGAGTA	30295	0.0	16.461403	10-11
TAGACCG	660	0.0	16.317017	5
GTACTTT	19590	0.0	15.4035015	14-15
TATGCCG	5970	0.0	15.387138	42-43
>>END_MODULE