FastQCFastQC Report
Fri 27 May 2016
SRR2139774_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139774_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9683356
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA135800.050.1048471
GTATCAA237600.034.94991
TCAACGC293550.027.6641484
ATCAACG299450.027.1469333
TATCAAC304700.026.9065172
CAACGCA302250.026.8808885
AACGCAG310000.026.2876666
GTACATG369250.024.2943841
TACATGG369700.023.8800072
ACATGGG376600.022.8268053
GTGGTAT57850.022.6946331
ACGCAGA363350.022.3944577
CGCAGAG372050.021.8598618
TGGTATC59900.020.9679372
CATGGGG260450.020.1467844
GCAGAGT427000.018.9705989
GAGTACT228950.018.72909512-13
CAGAGTA407600.017.9839810-11
CTTATAC205600.017.1601331
AGAGTAC384550.016.92692210-11
Produced by FastQC (version 0.11.5)