##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139774_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9683356
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.47210657131681	34.0	31.0	34.0	31.0	34.0
2	32.66017143230095	34.0	31.0	34.0	31.0	34.0
3	32.750454491190865	34.0	31.0	34.0	31.0	34.0
4	36.18327716134778	37.0	37.0	37.0	35.0	37.0
5	36.02666544532701	37.0	35.0	37.0	35.0	37.0
6	36.05871703983619	37.0	35.0	37.0	35.0	37.0
7	36.0708614864516	37.0	35.0	37.0	35.0	37.0
8	36.06862589788086	37.0	35.0	37.0	35.0	37.0
9	37.93729457018827	39.0	38.0	39.0	35.0	39.0
10-11	37.81716777736975	39.0	38.0	39.0	35.0	39.0
12-13	37.84313217442383	39.0	38.0	39.0	35.0	39.0
14-15	39.144542914667184	40.0	39.0	41.0	36.0	41.0
16-17	39.10780162373458	40.0	38.5	41.0	36.0	41.0
18-19	39.151344120777964	40.0	39.0	41.0	36.0	41.0
20-21	39.193540565187035	40.0	39.0	41.0	36.0	41.0
22-23	39.15280145339564	40.0	39.0	41.0	36.0	41.0
24-25	39.13684210321054	40.0	39.0	41.0	36.0	41.0
26-27	39.02685524525711	40.0	39.0	41.0	35.5	41.0
28-29	38.936297281990505	40.0	38.5	41.0	35.0	41.0
30-31	38.81339397552806	40.0	38.0	41.0	35.0	41.0
32-33	38.740478911280725	40.0	38.0	41.0	35.0	41.0
34-35	38.67552928341489	40.0	38.0	41.0	35.0	41.0
36-37	38.60743331832989	40.0	38.0	41.0	35.0	41.0
38-39	38.506764823963465	40.0	38.0	41.0	34.5	41.0
40-41	38.49420297435151	40.0	38.0	41.0	34.0	41.0
42-43	38.432230978693674	40.0	38.0	41.0	34.0	41.0
44-45	38.38646517721192	40.0	38.0	41.0	34.0	41.0
46-47	38.269228320652104	40.0	38.0	41.0	34.0	41.0
48-49	38.23092990565125	40.0	37.5	41.0	34.0	41.0
50-51	38.131881758215094	40.0	37.0	41.0	33.5	41.0
52-53	37.950891117117365	40.0	37.0	41.0	33.0	41.0
54-55	37.79127766128698	39.5	36.5	41.0	33.0	41.0
56-57	37.5863774003405	39.0	36.0	41.0	33.0	41.0
58-59	37.35204365544113	39.0	35.5	41.0	32.5	41.0
60-61	37.17627034286414	39.0	35.0	40.0	32.0	41.0
62-63	36.991938437443984	38.5	35.0	40.0	32.0	41.0
64-65	36.72579753045569	38.0	35.0	40.0	32.0	41.0
66-67	36.52594770770041	37.0	35.0	40.0	32.0	41.0
68-69	36.24907658549928	37.0	35.0	39.5	31.0	41.0
70-71	35.92443051043498	36.0	35.0	39.0	31.0	41.0
72-73	35.67242999787252	36.0	34.0	39.0	31.0	40.5
74-75	35.34144035276674	35.5	34.0	38.5	31.0	40.0
76-77	33.35805148951752	34.5	32.0	36.0	28.0	39.0
78-79	34.539232420666565	35.0	34.0	37.0	30.5	39.0
80-81	34.5344388060651	35.0	34.0	36.5	31.0	39.0
82-83	34.308058107426206	35.0	34.0	36.0	31.0	38.0
84-85	34.06547201789088	35.0	34.0	36.0	30.0	37.0
86-87	33.86888605450302	35.0	34.0	35.0	30.0	37.0
88-89	33.69058780745225	35.0	34.0	35.0	30.0	36.0
90-91	33.64940612152313	35.0	34.0	35.0	30.0	36.0
92-93	33.57914198643614	35.0	34.0	35.0	30.0	36.0
94-95	33.50420549726742	35.0	33.0	35.0	30.0	36.0
96-97	33.51080168013091	35.0	33.0	35.0	30.0	35.0
98-99	33.59474161617199	35.0	33.5	35.0	30.5	35.0
100-101	32.32583776635713	34.0	31.5	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	8.0
19	15.0
20	60.0
21	210.0
22	798.0
23	2611.0
24	6802.0
25	14498.0
26	27119.0
27	43915.0
28	65896.0
29	91823.0
30	122626.0
31	161862.0
32	215209.0
33	298533.0
34	447764.0
35	717918.0
36	1327493.0
37	2357603.0
38	2589734.0
39	1168341.0
40	22516.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.49913976104979	19.920314816474786	13.22601379108648	24.354531631388955
2	15.440808032739687	21.169881109206777	39.941818651987184	23.447492206066357
3	18.464734661319362	26.149478051217717	30.438543967273407	24.947243320189514
4	12.852756063670542	17.600514701364258	37.58492081974247	31.961808415222727
5	13.133836361467694	37.94584624853679	35.31113957920576	13.609177810789753
6	30.432344976288583	37.152919523687615	18.35054525266105	14.064190247362756
7	26.793531085710363	32.47612707825675	22.664921128583934	18.065420707448947
8	25.384332186520066	35.65840558360197	21.106651568593737	17.850610661284236
9	26.13806501529124	15.793495248841255	20.632877849151356	37.43556188671615
10-11	24.738749758927554	26.22000213253911	28.381193690059455	20.66005441847388
12-13	25.367295712575267	23.846823751551895	28.588786805216625	22.197093730656213
14-15	22.593786604381076	25.143554363016317	25.98798814769988	26.274670884902722
16-17	22.18265358326705	28.47144066587121	26.728318424869833	22.61758732599191
18-19	22.24829387662707	27.37728531306708	28.424184755780953	21.9502360545249
20-21	22.997630722704706	26.554675890064257	28.333097780879	22.114595606352037
22-23	22.819669350912207	26.668562639293047	28.207275081424914	22.304492928369832
24-25	22.561530952616238	26.770222851356994	28.40299839207658	22.265247803950192
26-27	22.48123379042291	26.981609817468737	28.327291542719692	22.209864849388662
28-29	22.215946705382965	26.857449848065496	28.322911160434995	22.603692286116544
30-31	22.662115238539315	26.829745986023195	28.294644778769605	22.21349399666789
32-33	22.559276783864956	26.82628004770366	28.42267373853375	22.191769429897636
34-35	22.220567147894617	26.893320739215852	28.6161469155859	22.269965197303634
36-37	22.487708373296428	26.97913506985176	28.211398359159816	22.32175819769199
38-39	22.16974084207589	26.978719625121293	28.37857641424985	22.47296311855297
40-41	22.32881452752828	26.833224798844462	28.40457950783697	22.433381165790287
42-43	22.441603267341613	26.848640768745412	28.21206036639321	22.497695597519762
44-45	22.255912161187574	27.11848378680311	28.002072205071375	22.62353184693794
46-47	22.662712958296467	26.874820932743425	27.551543209842123	22.910922899117985
48-49	22.477000721551537	27.010720315480096	27.56447929916443	22.947799663803934
50-51	22.3902771473767	27.233596072015796	27.57241865379662	22.803708126810882
52-53	22.52605724261535	27.23027053029566	27.591897353585825	22.651774873503165
54-55	22.40186753975129	27.14982308290878	27.380235204298863	23.068074173041065
56-57	22.6635963734989	27.368840492159503	27.087752732285157	22.87981040205644
58-59	22.42928396530526	27.452386366785085	27.486331101762307	22.63199856614735
60-61	22.5972619551794	27.431318977459124	27.222263432033472	22.749155635328005
62-63	22.60139347893589	27.654262662102003	27.157128433635336	22.587215425326775
64-65	22.380089403918888	27.4788123726373	27.165706097333718	22.97539212611009
66-67	22.49660359752559	27.38658182804372	27.084690008760692	23.032124565669996
68-69	22.476016064219575	27.50686423327921	27.18187807443078	22.83524162807043
70-71	22.491888528408268	27.346644884383664	27.262863500396744	22.898603086811324
72-73	22.48615667160839	27.351967729654103	26.88680520172136	23.275070397016147
74-75	22.444318537849465	27.367869537905577	26.87966750551503	23.308144418729928
76-77	22.667044019447175	26.96552118670811	27.105497000326967	23.261937793517752
78-79	22.589381851962752	26.971836031879594	27.067110411498334	23.37167170465932
80-81	23.00373685981423	26.958006634243166	26.69696598930772	23.34129051663489
82-83	22.95098289097718	27.266894986481592	26.776062777374083	23.006059345167145
84-85	22.743037014711597	26.86066637028912	26.796910622008618	23.599385992990666
86-87	22.576333577278323	27.307785752252535	26.842912903478272	23.272967766990867
88-89	23.05918812330727	26.836150181274327	26.812611293085176	23.292050402333224
90-91	23.185907807157367	27.081234946690426	26.84627704951832	22.886580196633894
92-93	22.762170316557413	27.05427949611552	26.900819247050904	23.282730940276164
94-95	23.15343528155279	26.666632639975706	26.990960171545563	23.188971906925943
96-97	23.03361718406754	27.01021222869177	27.05297263138379	22.903197955856903
98-99	23.347066486232247	27.310951339715317	27.243053387329187	22.09892878672325
100-101	27.444173126351913	34.344533353143305	27.92058586807407	10.290707652430708
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3298.0
1	2782.5
2	3373.0
3	4775.5
4	6265.0
5	9728.0
6	12909.0
7	15598.0
8	17947.5
9	18178.5
10	16408.5
11	14547.5
12	15445.0
13	20257.5
14	30866.0
15	44425.5
16	55077.5
17	61219.0
18	62248.5
19	58953.5
20	53829.5
21	48526.0
22	45757.0
23	48518.0
24	56285.5
25	68196.0
26	84001.0
27	101291.0
28	119775.5
29	139396.5
30	158229.0
31	177159.0
32	197236.0
33	216910.0
34	233832.5
35	249758.5
36	267722.0
37	283065.5
38	296429.5
39	310293.5
40	323763.0
41	336694.5
42	347569.5
43	360057.0
44	374559.5
45	387891.0
46	399109.0
47	409168.0
48	419950.0
49	433068.5
50	445716.0
51	444788.0
52	430604.5
53	419125.0
54	406023.5
55	382989.5
56	353727.0
57	321969.5
58	289697.5
59	254296.5
60	216541.5
61	178509.5
62	142742.5
63	112060.0
64	86053.5
65	65811.0
66	50151.5
67	38373.0
68	29251.5
69	21138.5
70	15209.5
71	11138.0
72	7992.5
73	5670.0
74	4009.5
75	2799.5
76	1943.5
77	1304.5
78	887.5
79	623.0
80	446.0
81	319.5
82	240.0
83	171.5
84	120.0
85	86.0
86	60.5
87	41.5
88	26.5
89	16.5
90	12.5
91	11.0
92	8.0
93	4.0
94	2.5
95	1.5
96	1.0
97	1.0
98	0.5
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0019001676691428053
3	0.0022925936008136023
4	7.332168723322782E-4
5	1.0326998201863073E-5
6	4.853689154875644E-4
7	0.0
8	1.0326998201863073E-5
9	3.5111793886334446E-4
10-11	6.557643858183051E-4
12-13	1.4457797482608304E-4
14-15	3.614449370652076E-5
16-17	4.3373392447824906E-4
18-19	0.0
20-21	7.745599002284228E-5
22-23	1.032852241087957E-5
24-25	0.0
26-27	9.302474613288379E-5
28-29	2.689007492505684E-4
30-31	7.457113827664028E-4
32-33	3.2225073217185505E-4
34-35	0.0010236750445938442
36-37	1.3675315093724034E-4
38-39	1.5403563811015904E-4
40-41	3.4347371497628045E-4
42-43	0.0012695075569336164
44-45	4.6705719241701326E-4
46-47	1.2825353426312684E-4
48-49	3.7336122002227607E-4
50-51	0.0
52-53	4.35308461316923E-4
54-55	6.636182121033038E-4
56-57	8.272820232390067E-4
58-59	7.606267250304809E-4
60-61	8.891495978364188E-4
62-63	8.652684340804515E-5
64-65	8.592958816645451E-4
66-67	0.0027661231999405473
68-69	0.0013340691005052042
70-71	2.893540210936451E-4
72-73	0.0
74-75	1.082936927655143E-4
76-77	2.0602853220466325E-5
78-79	0.0010524749366336904
80-81	1.274507179936198E-4
82-83	1.079082969394617E-4
84-85	9.788252344067297E-4
86-87	2.3739141846342913E-4
88-89	7.542097537271349E-6
90-91	3.837925628830681E-5
92-93	1.4857275924714898E-4
94-95	6.539461904364676E-4
96-97	2.4481140586132367E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	281.0
20-21	813.0
22-23	1767.0
24-25	4343.0
26-27	5451.0
28-29	7730.0
30-31	33341.0
32-33	42911.0
34-35	59635.0
36-37	87943.0
38-39	98267.0
40-41	98670.0
42-43	111000.0
44-45	131029.0
46-47	130765.0
48-49	121187.0
50-51	109477.0
52-53	97959.0
54-55	110206.0
56-57	118513.0
58-59	70496.0
60-61	123348.0
62-63	117412.0
64-65	121594.0
66-67	128471.0
68-69	118847.0
70-71	113182.0
72-73	104934.0
74-75	107545.0
76-77	105001.0
78-79	111433.0
80-81	112537.0
82-83	106236.0
84-85	104568.0
86-87	108419.0
88-89	114836.0
90-91	119535.0
92-93	121206.0
94-95	138790.0
96-97	180711.0
98-99	775641.0
100-101	5207326.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.49685864123656
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.14281522923427	77.06927531176947
2	6.893394211187316	11.787271008644007
3	1.6559646469666809	4.247393260097866
4	0.6001966735743194	2.0525972063009594
5	0.2756345100519456	1.1782942371278846
6	0.14641245780972215	0.7510683125204296
7	0.07924824098501969	0.47428329599440144
8	0.04882374462598171	0.33394214340987133
9	0.037135649399930734	0.28574832305667597
>10	0.11726761823383546	1.5887076225160834
>50	0.002625683777088408	0.15099160689669414
>100	4.419897433771952E-4	0.05464397897981513
>500	3.93444104941132E-5	0.02578369268580383
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0326998201863073E-5
2	0.0	2.0653996403726147E-5	0.0	0.0	1.0326998201863073E-5
3	0.0	2.0653996403726147E-5	0.0	0.0	1.0326998201863073E-5
4	0.0	6.196198921117844E-5	0.0	2.0653996403726147E-5	2.0653996403726147E-5
5	0.0	8.261598561490459E-5	0.0	2.0653996403726147E-5	2.0653996403726147E-5
6	0.0	8.261598561490459E-5	0.0	2.0653996403726147E-5	3.098099460558922E-5
7	0.0	8.261598561490459E-5	0.0	2.0653996403726147E-5	3.098099460558922E-5
8	0.0	9.294298381676765E-5	0.0	2.0653996403726147E-5	4.130799280745229E-5
9	0.0	1.6523197122980917E-4	0.0	2.0653996403726147E-5	4.130799280745229E-5
10-11	1.0326998201863073E-5	1.858859676335353E-4	0.0	2.0653996403726147E-5	4.130799280745229E-5
12-13	1.0326998201863073E-5	2.271939604409876E-4	0.0	4.647149190838383E-5	4.647149190838383E-5
14-15	1.0326998201863073E-5	2.891559496521661E-4	0.0	5.67984901102469E-5	7.745248651397305E-5
16-17	2.5817495504657684E-5	3.0464644695496067E-4	0.0	1.4457797482608304E-4	1.0326998201863074E-4
18-19	4.647149190838383E-5	3.56281437964276E-4	0.0	3.872624325698652E-4	1.135969802204938E-4
20-21	5.163499100931537E-5	3.872624325698652E-4	0.0	9.345933372686081E-4	1.1876047932142533E-4
22-23	5.163499100931537E-5	4.3373392447824906E-4	0.0	0.0021221981304828615	1.4974147392701455E-4
24-25	5.163499100931537E-5	4.6987841818476987E-4	0.0	0.0044922442178104375	1.858859676335353E-4
26-27	5.163499100931537E-5	4.8536891548756447E-4	0.0	0.010698770137130144	2.0137646493632993E-4
28-29	5.163499100931537E-5	5.060229118912906E-4	0.0	0.05122191108124084	2.11703463138193E-4
30-31	5.163499100931537E-5	5.67984901102469E-4	0.0	0.12978971340101508	2.1686696223912453E-4
32-33	5.163499100931537E-5	5.67984901102469E-4	0.0	0.13688436116569502	2.4784795684471375E-4
34-35	5.163499100931537E-5	6.712548831210997E-4	0.0	0.14671566345386866	2.994829478540291E-4
36-37	5.163499100931537E-5	7.487073696350728E-4	0.0	0.16072423651469592	2.994829478540291E-4
38-39	5.163499100931537E-5	7.745248651397304E-4	0.0	0.18656238601575736	3.098099460558922E-4
40-41	5.163499100931537E-5	8.313233552499774E-4	0.0	0.24640734059555386	3.2013694425775526E-4
42-43	5.163499100931537E-5	8.623043498555665E-4	0.0	0.26791847785003464	3.2013694425775526E-4
44-45	5.163499100931537E-5	9.552473336723342E-4	0.0	0.2973504227253444	3.2013694425775526E-4
46-47	5.163499100931537E-5	0.0010223728219844442	0.0	0.32961712860706555	3.5111793886334446E-4
48-49	5.163499100931537E-5	0.0010430268183881704	0.0	0.3566273924040384	3.717719352670706E-4
50-51	5.163499100931537E-5	0.0011411333013058695	0.0	0.3848975499816386	3.717719352670706E-4
52-53	5.163499100931537E-5	0.0011514602995077328	0.0	0.42199212752273074	3.872624325698652E-4
54-55	5.163499100931537E-5	0.001203095290517048	0.0	0.4991451310888497	4.0275292987265986E-4
56-57	5.163499100931537E-5	0.0012495667824254319	0.0	0.5843377027551191	4.0275292987265986E-4
58-59	5.163499100931537E-5	0.001290874775232884	0.0	0.6602256490415099	4.0275292987265986E-4
60-61	5.163499100931537E-5	0.0013270192689394048	0.0	1.1910746646100794	4.0275292987265986E-4
62-63	5.67984901102469E-5	0.0013580002635449943	0.0	1.2028164615655976	4.1307992807452295E-4
64-65	7.228898741304152E-5	0.001404471755453378	0.0	1.214480806034602	4.1307992807452295E-4
66-67	7.228898741304152E-5	0.0014354527500589672	0.0	1.2234188229783145	4.1307992807452295E-4
68-69	7.228898741304152E-5	0.0014457797482608304	0.0	1.232506581395954	4.1307992807452295E-4
70-71	7.228898741304152E-5	0.0014561067464626933	0.0	1.2407836704547472	4.23406926276386E-4
72-73	7.228898741304152E-5	0.0014561067464626933	0.0	1.2468198009037363	4.750419172857014E-4
74-75	7.228898741304152E-5	0.0014922512401692142	0.0	1.2520452619938789	4.750419172857014E-4
76-77	7.228898741304152E-5	0.0015593767284813242	0.0	1.256558160208093	4.8536891548756447E-4
78-79	7.228898741304152E-5	0.0015851942239859817	0.0	1.260410130537388	4.956959136894275E-4
80-81	7.228898741304152E-5	0.0015903577230869132	0.0	1.2622121917236133	4.956959136894275E-4
82-83	7.228898741304152E-5	0.0015903577230869132	0.0	1.2628834466067342	5.163499100931537E-4
84-85	7.228898741304152E-5	0.0016006847212887763	0.0	1.2634617585060386	5.163499100931537E-4
86-87	7.228898741304152E-5	0.0016626467104999547	0.0	1.2636786254682777	5.266769082950167E-4
88-89	7.228898741304152E-5	0.0017555896943167225	0.0	1.2637664049529935	5.421674055978113E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13580	0.0	50.104847	1
GTATCAA	23760	0.0	34.9499	1
TCAACGC	29355	0.0	27.664148	4
ATCAACG	29945	0.0	27.146933	3
TATCAAC	30470	0.0	26.906517	2
CAACGCA	30225	0.0	26.880888	5
AACGCAG	31000	0.0	26.287666	6
GTACATG	36925	0.0	24.294384	1
TACATGG	36970	0.0	23.880007	2
ACATGGG	37660	0.0	22.826805	3
GTGGTAT	5785	0.0	22.694633	1
ACGCAGA	36335	0.0	22.394457	7
CGCAGAG	37205	0.0	21.859861	8
TGGTATC	5990	0.0	20.967937	2
CATGGGG	26045	0.0	20.146784	4
GCAGAGT	42700	0.0	18.970598	9
GAGTACT	22895	0.0	18.729095	12-13
CAGAGTA	40760	0.0	17.98398	10-11
CTTATAC	20560	0.0	17.160133	1
AGAGTAC	38455	0.0	16.926922	10-11
>>END_MODULE