FastQCFastQC Report
Fri 27 May 2016
SRR2139773_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139773_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8392702
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102600.049.9594351
GTATCAA182750.035.92841
TCAACGC223050.028.7442054
ATCAACG229200.027.9730953
CAACGCA231950.027.7129365
TATCAAC238250.027.3136482
AACGCAG239000.026.8954626
TATCTCG227250.026.64568194-95
GTACATG279250.023.4678331
TACATGG279250.023.1389852
ACGCAGA284200.022.6615837
ACATGGG282450.022.433353
CGCAGAG287550.022.3685448
CTATCTC252550.021.57586594-95
CATGGGG215600.020.2896184
GTGGTAT50350.019.5649641
GGACCGA16800.019.3794546
ACCGAAC14500.019.286588
GCAGAGT335100.019.1197579
GAGTACT186150.019.10391612-13
Produced by FastQC (version 0.11.5)