##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139773_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8392702
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.49304753105734	34.0	31.0	34.0	31.0	34.0
2	32.691159414453175	34.0	31.0	34.0	31.0	34.0
3	32.78495685894721	34.0	31.0	34.0	31.0	34.0
4	36.20549389219348	37.0	37.0	37.0	35.0	37.0
5	36.0410774742151	37.0	35.0	37.0	35.0	37.0
6	36.06469692358909	37.0	35.0	37.0	35.0	37.0
7	36.086401137559754	37.0	35.0	37.0	35.0	37.0
8	36.08758395091354	37.0	35.0	37.0	35.0	37.0
9	37.97259106781106	39.0	38.0	39.0	35.0	39.0
10-11	37.853946381034376	39.0	38.0	39.0	35.0	39.0
12-13	37.88314079303662	39.0	38.0	39.0	35.0	39.0
14-15	39.19686180922425	40.0	39.0	41.0	36.0	41.0
16-17	39.16437834918957	40.0	39.0	41.0	36.0	41.0
18-19	39.2025590209208	40.0	39.0	41.0	36.0	41.0
20-21	39.24683248068377	40.0	39.0	41.0	36.0	41.0
22-23	39.2101212679789	40.0	39.0	41.0	36.0	41.0
24-25	39.1971560034223	40.0	39.0	41.0	36.0	41.0
26-27	39.09218241432505	40.0	39.0	41.0	36.0	41.0
28-29	39.016028416848854	40.0	39.0	41.0	35.5	41.0
30-31	38.9010165370069	40.0	38.0	41.0	35.0	41.0
32-33	38.825170807640276	40.0	38.0	41.0	35.0	41.0
34-35	38.76404418981075	40.0	38.0	41.0	35.0	41.0
36-37	38.693875704784226	40.0	38.0	41.0	35.0	41.0
38-39	38.59800695786283	40.0	38.0	41.0	35.0	41.0
40-41	38.58738530200584	40.0	38.0	41.0	35.0	41.0
42-43	38.5277976429971	40.0	38.0	41.0	34.5	41.0
44-45	38.49164014962662	40.0	38.0	41.0	34.0	41.0
46-47	38.37994310299666	40.0	38.0	41.0	34.0	41.0
48-49	38.35477772372256	40.0	38.0	41.0	34.0	41.0
50-51	38.25446930275666	40.0	37.5	41.0	34.0	41.0
52-53	38.077725532256935	40.0	37.0	41.0	33.0	41.0
54-55	37.93971111521208	40.0	37.0	41.0	33.0	41.0
56-57	37.7368189125566	39.0	36.0	41.0	33.0	41.0
58-59	37.519641391082956	39.0	36.0	41.0	33.0	41.0
60-61	37.35258724346295	39.0	35.5	41.0	32.5	41.0
62-63	37.17233919952525	39.0	35.0	40.0	32.0	41.0
64-65	36.910448662804896	38.0	35.0	40.0	32.0	41.0
66-67	36.69498579037263	37.5	35.0	40.0	32.0	41.0
68-69	36.405263742516965	37.0	35.0	40.0	32.0	41.0
70-71	36.05885598329016	36.5	35.0	39.0	31.0	41.0
72-73	35.81063058622583	36.0	35.0	39.0	31.0	41.0
74-75	35.46111563982095	35.5	34.0	38.5	31.0	40.0
76-77	33.47006329610926	34.5	32.0	36.5	28.0	39.0
78-79	34.572658474598065	35.0	34.0	37.0	30.5	39.0
80-81	34.528480020585775	35.0	34.0	36.5	31.0	39.0
82-83	34.28499890791272	35.0	34.0	36.0	30.5	37.5
84-85	34.04423388396044	35.0	34.0	36.0	30.0	37.0
86-87	33.85049587135335	35.0	34.0	35.0	30.0	37.0
88-89	33.66200052254662	35.0	33.5	35.0	30.0	36.0
90-91	33.64907353669244	35.0	34.0	35.0	30.0	36.0
92-93	33.598607039268394	35.0	34.0	35.0	30.0	36.0
94-95	33.52353188656062	35.0	33.0	35.0	30.0	36.0
96-97	33.50988511778412	35.0	33.0	35.0	30.0	35.0
98-99	33.58702204258702	35.0	33.0	35.0	30.0	35.0
100-101	32.25528565253667	34.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	7.0
19	10.0
20	55.0
21	165.0
22	636.0
23	2226.0
24	5602.0
25	12127.0
26	22434.0
27	37486.0
28	55440.0
29	78353.0
30	104437.0
31	137345.0
32	182460.0
33	252816.0
34	378955.0
35	599904.0
36	1108544.0
37	2099500.0
38	2363662.0
39	931450.0
40	19087.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.78106145076996	19.507769964905226	13.048157792329572	24.663010791995234
2	15.424209122689453	21.03224444259889	39.8467592734363	23.696787161275356
3	18.561140809599433	25.749014357588017	30.403791672942116	25.286053159870438
4	12.956504280821408	17.4391209466838	37.35465433208641	32.24972044040838
5	13.273188214378184	37.809174900904964	35.412616272163156	13.505020612553695
6	31.013767620599086	36.84668382371603	18.17146714934223	13.968081406342659
7	27.08002738569772	32.30330351298068	22.512618701343143	18.10405039997846
8	25.50791759505284	35.667520583364116	21.027821797514505	17.796740024068537
9	26.216815806854154	15.811608305969155	20.402213218855564	37.569362668321126
10-11	24.873556208983512	26.076297365484276	28.50385253212866	20.546293893403547
12-13	25.531508511720162	23.661137352524232	28.581471321628904	22.22588281412669
14-15	22.592677123731168	24.971828194253828	25.942058332959494	26.49343634905551
16-17	22.42251079022125	28.24535485942284	26.58760440734672	22.744529943009187
18-19	22.56085703984247	27.301177856666424	28.218659497263214	21.919305606227887
20-21	23.10687913303313	26.44177223078365	28.147569442582615	22.303779193600604
22-23	22.75161146934782	26.643275683109273	28.028471973124898	22.576640874418004
24-25	22.708100491979092	26.776856568255553	28.087934564593347	22.42710837517201
26-27	22.602983085844105	26.90074040431099	28.116119603002648	22.38015690684226
28-29	22.49545459156365	26.79791415428551	28.15968092933389	22.546950324816944
30-31	22.59906517986296	26.550062449677377	28.273909091539533	22.576963278920132
32-33	22.2243280537557	27.00440364770818	28.084221262299014	22.6870470362371
34-35	22.63734287261963	26.859392026609513	28.11655572840278	22.386709372368074
36-37	22.49767046504047	26.915740617356498	28.19721543204348	22.389373485559553
38-39	22.374538154947196	26.862430273455146	28.16936794025911	22.59366363133855
40-41	22.48629389546213	26.58332283647405	28.26337289601651	22.66701037204731
42-43	22.751306379332426	26.632238723043972	28.060114710972815	22.55634018665079
44-45	22.33793921346708	26.89520347308429	27.93139738989629	22.83545992355234
46-47	22.699872854495396	26.667708609281664	27.54070037816901	23.09171815805393
48-49	22.500999978212853	27.046406035957066	27.49034011672091	22.962253869109173
50-51	22.356790310259957	27.176084134956458	27.649139074303957	22.81798648047963
52-53	22.50985479291941	26.998655427620445	27.412217598688436	23.07927218077171
54-55	22.319978523288132	26.760230471004753	27.441402741086446	23.47838826462067
56-57	22.633687895119873	26.487158888120803	27.572200062064507	23.30695315469482
58-59	22.150181206549536	26.4012933076872	28.08404664997421	23.364478835789058
60-61	22.452475814883893	26.291895373434127	28.03829834478229	23.217330466899693
62-63	22.010001004138648	27.05980885590665	28.066413811635787	22.86377632831892
64-65	21.461917316097146	27.27263108455463	27.852515224526552	23.412936374821676
66-67	21.633893355787517	27.908708205371525	27.217088483510565	23.240309955330392
68-69	21.832683636853893	28.155781571694288	26.723888390235945	23.287646401215873
70-71	21.79834388265531	27.723542003045893	26.686417518651762	23.791696595647036
72-73	21.689676822906215	27.154609266122886	26.594535970853812	24.56117794011709
74-75	22.261650154179865	26.69916752026868	26.70161833492833	24.337563990623124
76-77	22.80213558427476	26.469460315616576	26.618621309350623	24.109782790758036
78-79	23.075981958455387	26.24853026136074	25.691820739397315	24.983667040786557
80-81	22.915216287119765	26.83132964634592	25.61063065108825	24.642823415446063
82-83	22.847394737366745	27.032553615915255	25.69471911135747	24.42533253536053
84-85	22.851438375595514	27.010842491807	25.64901365596096	24.488705476636525
86-87	23.325810205167222	27.401048569692122	25.581879394444123	23.691261830696536
88-89	23.48581085980641	27.00588699978671	26.05330338180183	23.45499875860505
90-91	23.22734361001659	26.97513836489905	26.36363681694745	23.43388120813691
92-93	22.900160053617917	26.251212370420046	26.228654477961566	24.619973098000468
94-95	22.820330620502	26.20376360968457	27.13190385471146	23.844001915101973
96-97	22.216016633713526	26.530676325103258	27.48707087622519	23.76623616495803
98-99	22.52085128705743	26.502155182119115	27.959661365154233	23.017332165669224
100-101	27.18051134390378	33.80698340581427	28.762344111471695	10.250161138810256
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2189.0
1	1920.5
2	2514.0
3	3655.5
4	4871.5
5	7428.0
6	9771.5
7	11931.5
8	13823.0
9	14179.0
10	13086.5
11	11787.0
12	12421.0
13	15570.0
14	22717.0
15	32166.0
16	39852.5
17	44407.0
18	45226.5
19	42872.5
20	39357.0
21	36156.5
22	34600.0
23	36575.0
24	42388.5
25	51401.0
26	63038.0
27	76198.0
28	91671.0
29	108773.5
30	124608.0
31	140525.0
32	157006.5
33	172806.5
34	187609.0
35	200746.0
36	215639.5
37	230973.5
38	245740.0
39	259045.0
40	271888.0
41	286248.0
42	296826.5
43	307028.5
44	319570.0
45	332353.5
46	344191.5
47	354855.5
48	373419.0
49	382858.0
50	378161.0
51	375500.5
52	368744.5
53	358184.5
54	344347.0
55	325666.5
56	303640.0
57	277187.0
58	248442.5
59	217547.5
60	185396.0
61	154792.0
62	124767.5
63	97534.0
64	74079.0
65	56639.5
66	44248.5
67	34312.0
68	26719.5
69	19765.5
70	14039.5
71	10202.0
72	7294.0
73	5189.5
74	3679.5
75	2578.5
76	1796.0
77	1219.0
78	833.0
79	580.0
80	411.5
81	291.5
82	207.5
83	152.5
84	115.0
85	84.5
86	64.0
87	47.0
88	28.0
89	16.0
90	9.5
91	4.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0018468426497211506
3	0.002454513457048755
4	4.408592131592424E-4
5	1.1915113869168713E-5
6	4.6468944089757985E-4
7	0.0
8	2.3830227738337425E-5
9	4.6468944089757985E-4
10-11	4.7660455476674854E-4
12-13	1.1319358175710278E-4
14-15	2.9787784672921784E-5
16-17	3.991563146171519E-4
18-19	0.0
20-21	8.936762929306452E-5
22-23	1.7875419409475985E-5
24-25	0.0
26-27	1.1330637238020014E-4
28-29	2.1481372129805251E-4
30-31	6.994601901037144E-4
32-33	2.8797613253813526E-4
34-35	9.764376552694091E-4
36-37	1.7597393838108709E-4
38-39	1.5311872213239256E-4
40-41	3.266135371204604E-4
42-43	0.001357242635822477
44-45	4.219782124461277E-4
46-47	2.1698227057012666E-4
48-49	3.167853706705622E-4
50-51	0.0
52-53	4.953211305579324E-4
54-55	6.749475674922754E-4
56-57	7.162768407336558E-4
58-59	7.579163867971763E-4
60-61	7.753561341063991E-4
62-63	4.065337012372989E-5
64-65	7.763699373895434E-4
66-67	0.0025456291825907977
68-69	0.001607339209963747
70-71	3.089279087987338E-4
72-73	0.0
74-75	1.5502116887880645E-4
76-77	0.0
78-79	9.638781721742944E-4
80-81	1.540564527546603E-4
82-83	1.2509028978025625E-4
84-85	8.565486435164304E-4
86-87	3.2194731766775245E-4
88-89	2.452541282401136E-5
90-91	3.324280210227481E-5
92-93	1.691627618967307E-4
94-95	7.238453976530864E-4
96-97	1.7590641497273188E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	273.0
20-21	722.0
22-23	1482.0
24-25	4705.0
26-27	4768.0
28-29	10801.0
30-31	27890.0
32-33	35730.0
34-35	48839.0
36-37	72113.0
38-39	52148.0
40-41	81557.0
42-43	87886.0
44-45	102525.0
46-47	102912.0
48-49	96182.0
50-51	87909.0
52-53	13888.0
54-55	16863.0
56-57	18156.0
58-59	19098.0
60-61	102488.0
62-63	101084.0
64-65	104221.0
66-67	111207.0
68-69	102856.0
70-71	97958.0
72-73	90346.0
74-75	93060.0
76-77	91073.0
78-79	96344.0
80-81	96911.0
82-83	91784.0
84-85	91418.0
86-87	94892.0
88-89	98711.0
90-91	104704.0
92-93	106080.0
94-95	113770.0
96-97	161918.0
98-99	766845.0
100-101	4788585.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.07740081985112
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.11716585919194	74.92739673872433
2	7.533263430199398	12.66754417804803
3	1.8668492898317517	4.708795080343158
4	0.694945474648515	2.3371683687985954
5	0.31252408850322777	1.3138106527472257
6	0.1564971447474769	0.7894723899657525
7	0.08578696416423913	0.5048921479808457
8	0.05874973969169808	0.3951620329696653
9	0.04097023703769582	0.3100203936992395
>10	0.12889321897047967	1.7213033881118267
>50	0.00364971060973795	0.21184925724448736
>100	6.577179740120618E-4	0.08815902872750685
>500	4.7124429790363866E-5	0.024426342639354465
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.766045547667485E-5	0.0	0.0	0.0
3	0.0	4.766045547667485E-5	0.0	0.0	0.0
4	0.0	5.957556934584357E-5	0.0	0.0	0.0
5	0.0	5.957556934584357E-5	0.0	1.1915113869168713E-5	0.0
6	0.0	5.957556934584357E-5	0.0	1.1915113869168713E-5	0.0
7	0.0	5.957556934584357E-5	0.0	1.1915113869168713E-5	0.0
8	0.0	5.957556934584357E-5	0.0	1.1915113869168713E-5	1.1915113869168713E-5
9	0.0	1.5489648029919328E-4	0.0	4.766045547667485E-5	1.1915113869168713E-5
10-11	0.0	1.5489648029919328E-4	0.0	6.553312628042793E-5	1.1915113869168713E-5
12-13	0.0	1.5489648029919328E-4	0.0	8.936335401876536E-5	1.1915113869168713E-5
14-15	0.0	1.906418219066994E-4	0.0	1.4298136643002457E-4	1.7872670803753067E-5
16-17	0.0	1.906418219066994E-4	0.0	2.2638716351420555E-4	2.3830227738337425E-5
18-19	0.0	2.0255693577586812E-4	0.0	5.123498963742547E-4	2.3830227738337425E-5
20-21	0.0	2.1447204964503684E-4	0.0	0.001471516562842336	2.3830227738337425E-5
22-23	0.0	2.1447204964503684E-4	0.0	0.0032468685293484748	3.5745341607506135E-5
24-25	0.0	2.1447204964503684E-4	0.0	0.005802660454285164	4.766045547667485E-5
26-27	0.0	2.1447204964503684E-4	0.0	0.011277655277168187	4.766045547667485E-5
28-29	0.0	2.1447204964503684E-4	0.0	0.0504605072359295	4.766045547667485E-5
30-31	0.0	2.8596273286004913E-4	0.0	0.07567884574002509	4.766045547667485E-5
32-33	0.0	2.8596273286004913E-4	0.0	0.0901795393188034	4.766045547667485E-5
34-35	0.0	3.872412007479832E-4	0.0	0.10908882502917416	4.766045547667485E-5
36-37	0.0	4.885196686359172E-4	0.0	0.13429524841940055	4.766045547667485E-5
38-39	0.0	5.004347825050859E-4	0.0	0.1950265838105535	4.766045547667485E-5
40-41	0.0	5.361801241125921E-4	0.0	0.6398356572174253	4.766045547667485E-5
42-43	0.0	5.480952379817608E-4	0.0	0.6930306830863291	4.766045547667485E-5
44-45	0.0	6.612888197388636E-4	0.0	0.7772943683690903	4.766045547667485E-5
46-47	0.0	7.625672876267976E-4	0.0	0.8756298031313396	4.766045547667485E-5
48-49	0.0	7.68524844561382E-4	0.0	0.9548355225766386	4.766045547667485E-5
50-51	0.0	8.817184263184847E-4	0.0	1.0301271271159158	4.766045547667485E-5
52-53	0.0	8.936335401876535E-4	0.0	1.305509238860143	4.766045547667485E-5
54-55	0.0	8.936335401876535E-4	0.0	2.2317663608215805	4.766045547667485E-5
56-57	0.0	8.936335401876535E-4	0.0	3.2366036587501856	4.766045547667485E-5
58-59	0.0	8.936335401876535E-4	0.0	4.261077064335181	4.766045547667485E-5
60-61	0.0	8.995910971222379E-4	0.0	5.037156091089615	4.766045547667485E-5
62-63	0.0	9.174637679259909E-4	0.0	5.058174351954829	4.766045547667485E-5
64-65	0.0	9.770393372718345E-4	0.0	5.069553285699886	4.766045547667485E-5
66-67	0.0	0.0010008695650101718	0.0	5.078048761888603	4.766045547667485E-5
68-69	0.0	0.0010187422358139248	0.0	5.0859723126116005	4.766045547667485E-5
70-71	0.0	0.0010425724635522624	0.0	5.0930022297944095	4.766045547667485E-5
72-73	0.0	0.0010485300204868468	0.0	5.098697654223872	4.766045547667485E-5
74-75	0.0	0.0010664026912906	0.0	5.103153906810942	4.766045547667485E-5
76-77	0.0	0.0010723602482251842	0.0	5.107735268093636	4.766045547667485E-5
78-79	0.0	0.0010723602482251842	0.0	5.111476613848556	4.766045547667485E-5
80-81	0.0	0.0010723602482251842	0.0	5.113079196663959	4.766045547667485E-5
82-83	0.0	0.0010723602482251842	0.0	5.113788145939175	4.766045547667485E-5
84-85	0.0	0.0010723602482251842	0.0	5.114425604531174	5.361801241125921E-5
86-87	0.0	0.001084275362094353	0.0	5.1147949730611195	5.957556934584357E-5
88-89	0.0	0.0010961904759635216	0.0	5.1148843364151375	5.957556934584357E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10260	0.0	49.959435	1
GTATCAA	18275	0.0	35.9284	1
TCAACGC	22305	0.0	28.744205	4
ATCAACG	22920	0.0	27.973095	3
CAACGCA	23195	0.0	27.712936	5
TATCAAC	23825	0.0	27.313648	2
AACGCAG	23900	0.0	26.895462	6
TATCTCG	22725	0.0	26.645681	94-95
GTACATG	27925	0.0	23.467833	1
TACATGG	27925	0.0	23.138985	2
ACGCAGA	28420	0.0	22.661583	7
ACATGGG	28245	0.0	22.43335	3
CGCAGAG	28755	0.0	22.368544	8
CTATCTC	25255	0.0	21.575865	94-95
CATGGGG	21560	0.0	20.289618	4
GTGGTAT	5035	0.0	19.564964	1
GGACCGA	1680	0.0	19.379454	6
ACCGAAC	1450	0.0	19.28658	8
GCAGAGT	33510	0.0	19.119757	9
GAGTACT	18615	0.0	19.103916	12-13
>>END_MODULE