FastQCFastQC Report
Fri 27 May 2016
SRR2139772_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139772_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9486639
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90250.043.4687541
GTATCAA154700.032.8933451
TCAACGC177800.027.5456924
ATCAACG181100.027.1110293
CAACGCA185700.026.4178685
TATCAAC191800.025.9590722
AACGCAG191000.025.7273336
GTACATG232950.021.8616091
ACGCAGA225450.021.8140667
TACATGG231050.021.7074242
CGCAGAG228600.021.5312428
ACATGGG237300.020.3480243
CTTATAC161550.019.9097391
CATGGGG183500.018.2359874
TATACAC195950.018.0099563
GCAGAGT283800.017.2718019
GAGTACT161400.017.0591512-13
TTATACA196500.017.050562
AGAGTAC244950.016.3304910-11
CAGAGTA273450.015.99477910-11
Produced by FastQC (version 0.11.5)