##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139772_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9486639
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.47688090587193	34.0	31.0	34.0	31.0	34.0
2	32.67041109079833	34.0	31.0	34.0	31.0	34.0
3	32.76670177920758	34.0	31.0	34.0	31.0	34.0
4	36.18761017468884	37.0	37.0	37.0	35.0	37.0
5	36.027818703757994	37.0	35.0	37.0	35.0	37.0
6	36.051164590536224	37.0	35.0	37.0	35.0	37.0
7	36.07154051081737	37.0	35.0	37.0	35.0	37.0
8	36.065657183750744	37.0	35.0	37.0	35.0	37.0
9	37.942500394502204	39.0	38.0	39.0	35.0	39.0
10-11	37.8213279223548	39.0	38.0	39.0	35.0	39.0
12-13	37.85306139508418	39.0	38.0	39.0	35.0	39.0
14-15	39.173067616465644	40.0	39.0	41.0	36.0	41.0
16-17	39.13810670986848	40.0	39.0	41.0	36.0	41.0
18-19	39.168812526754735	40.0	39.0	41.0	36.0	41.0
20-21	39.211075032533	40.0	39.0	41.0	36.0	41.0
22-23	39.170117215042566	40.0	39.0	41.0	36.0	41.0
24-25	39.15928857857638	40.0	39.0	41.0	36.0	41.0
26-27	39.0562943037399	40.0	39.0	41.0	36.0	41.0
28-29	38.973768230567835	40.0	38.5	41.0	35.5	41.0
30-31	38.864795652843256	40.0	38.0	41.0	35.0	41.0
32-33	38.80522556614605	40.0	38.0	41.0	35.0	41.0
34-35	38.74130105091949	40.0	38.0	41.0	35.0	41.0
36-37	38.67798894364768	40.0	38.0	41.0	35.0	41.0
38-39	38.58111332122975	40.0	38.0	41.0	35.0	41.0
40-41	38.579761150930665	40.0	38.0	41.0	35.0	41.0
42-43	38.534414613967364	40.0	38.0	41.0	34.5	41.0
44-45	38.50625671710078	40.0	38.0	41.0	34.0	41.0
46-47	38.40677870786993	40.0	38.0	41.0	34.0	41.0
48-49	38.39935144501083	40.0	38.0	41.0	34.0	41.0
50-51	38.312937237509374	40.0	38.0	41.0	34.0	41.0
52-53	38.14892157923089	40.0	37.0	41.0	33.5	41.0
54-55	38.01152375725744	40.0	37.0	41.0	33.0	41.0
56-57	37.80927538852235	39.5	37.0	41.0	33.0	41.0
58-59	37.596835767740075	39.0	36.0	41.0	33.0	41.0
60-61	37.43610165709174	39.0	36.0	41.0	32.5	41.0
62-63	37.272112004963525	39.0	35.0	40.5	32.0	41.0
64-65	37.01378513541215	38.5	35.0	40.0	32.0	41.0
66-67	36.81938032923944	38.0	35.0	40.0	32.0	41.0
68-69	36.545861536492566	37.0	35.0	40.0	32.0	41.0
70-71	36.21278585537913	37.0	35.0	39.5	31.0	41.0
72-73	35.954755372653416	36.5	35.0	39.0	31.0	41.0
74-75	35.599696399639576	36.0	34.5	39.0	31.0	40.0
76-77	33.58849138937251	34.5	32.0	36.5	28.0	39.0
78-79	34.72825626946535	35.0	34.0	37.0	30.5	39.0
80-81	34.70204559444631	35.0	34.0	37.0	31.0	39.0
82-83	34.45763637751694	35.0	34.0	36.0	31.0	38.5
84-85	34.18748511077152	35.0	34.0	36.0	30.5	37.0
86-87	33.97678099860107	35.0	34.0	35.5	30.0	37.0
88-89	33.77267755808791	35.0	34.0	35.0	30.0	36.5
90-91	33.724630599473656	35.0	34.0	35.0	30.0	36.0
92-93	33.64779963316042	35.0	34.0	35.0	30.0	36.0
94-95	33.569595900175344	35.0	34.0	35.0	30.0	36.0
96-97	33.574786378195434	35.0	34.0	35.0	30.0	35.5
98-99	33.63781465766335	35.0	34.0	35.0	30.5	35.0
100-101	32.411604403967914	34.0	31.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	5.0
19	15.0
20	44.0
21	196.0
22	743.0
23	2458.0
24	6349.0
25	13511.0
26	25084.0
27	41535.0
28	62164.0
29	86495.0
30	116016.0
31	152725.0
32	202458.0
33	276221.0
34	410557.0
35	642288.0
36	1165956.0
37	2221433.0
38	2748008.0
39	1287062.0
40	25315.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.080625182427625	19.914766441518434	13.108119746097643	24.896488629956302
2	15.14578927040219	21.24344537545834	40.1264774785715	23.48428787556797
3	18.447694251681057	25.566969621806614	30.52754335845374	25.457792768058585
4	12.661338438088332	17.73550636794293	38.023175896898564	31.579979297070178
5	13.152366460815662	37.631372978932994	35.89835595023947	13.317904610011874
6	30.65411381056618	37.5567616068381	18.35422489349721	13.434899689098504
7	26.788064771938725	32.222571133991714	22.85179187275915	18.137572221310414
8	24.839835233497393	36.408139154054275	21.303914126786974	17.448111485661357
9	25.537257703021588	16.156132617348128	20.796708874262883	37.5099008053674
10-11	24.343792515666742	26.420379364463038	29.006733883398294	20.229094236471926
12-13	24.941081513354042	23.91300234482796	28.98286202672637	22.163054115091636
14-15	22.039112598021436	25.49389343630907	26.15481788513769	26.312176080531806
16-17	22.072849508327433	28.714835391991944	26.909462169684012	22.30285292999661
18-19	22.08517157657206	27.871794215000694	28.31989285140923	21.723141357018015
20-21	22.623979928659242	27.144295704401017	28.206590388895403	22.025133978044337
22-23	22.352257816386015	27.393781724630678	28.054357248149685	22.199603210833622
24-25	22.27869952146944	27.486184917571403	28.16070662692639	22.074408934032764
26-27	22.284501001538217	27.64541427061267	28.07304426524085	21.997040462608265
28-29	22.0745099478339	27.469375287277874	28.1507581495833	22.305356615304923
30-31	22.5141621590961	27.473559498582233	28.057485817734158	21.954792524587507
32-33	22.288021954580238	27.613955526341126	28.121053522892215	21.97696899618642
34-35	22.286643508513556	27.659708548773526	28.202004194080267	21.851643748632654
36-37	22.34355812132391	27.477160761456272	28.06789317119611	22.11138794602371
38-39	22.06569694350007	27.41051609831241	28.30224378087284	22.221543177314686
40-41	22.20782734363331	27.272854884051256	28.28279084131135	22.236526931004086
42-43	22.270008405968714	27.316532776793096	28.20900322149356	22.204455595744626
44-45	22.127570460742092	27.536062891615742	28.03277795300917	22.303588694632996
46-47	22.42250466109886	27.352596571941966	27.625510504352434	22.599388262606734
48-49	22.2071945625773	27.4881577842716	27.746382855466255	22.558264797684842
50-51	22.109511162703573	27.65140079541673	27.799912961102123	22.439175080777567
52-53	22.23853169538601	27.65072409583943	27.83792573952193	22.272818469252634
54-55	22.159742175019627	27.543871044407283	27.675405605304725	22.620981175268366
56-57	22.380952409838184	27.721275171779038	27.408571903280727	22.489200515102052
58-59	22.098829148145917	27.814128959790953	27.781686631964735	22.3053552600984
60-61	22.352813346510956	27.95193995877458	27.380378507961368	22.314868186753095
62-63	22.15756639402521	28.17023337834265	27.382803484449624	22.289396743182515
64-65	22.11044116304196	28.085904694326814	27.361890390115494	22.441763752515733
66-67	22.18551193027159	28.02420088279301	27.26447483429915	22.525812352636247
68-69	22.244600287655395	27.939987219741454	27.280720516616658	22.534691975986497
70-71	22.262440955679626	27.694781365948913	27.34095029955162	22.70182737881984
72-73	22.288237136995225	27.689304427530885	27.281679547511615	22.74077888796227
74-75	22.186987334523614	27.790123250323322	27.27598056179653	22.746908853356533
76-77	22.420031939343414	27.761520977445304	27.155307169734797	22.66313991347649
78-79	22.412606484113894	27.70932549126558	27.085557782506115	22.792510242114407
80-81	22.433345112884787	27.761351417509793	27.093524895748068	22.71177857385735
82-83	22.395571546896793	27.762702320356986	27.19945589717088	22.642270235575346
84-85	22.53593253133713	27.747225802993192	27.138069653910602	22.57877201175907
86-87	22.418274353865858	27.74048674471974	27.2061794546634	22.635059446751
88-89	22.421630133403493	27.782038993796704	27.19587647186767	22.600454400932136
90-91	22.43721803264491	27.829858679765245	27.21997693571282	22.512946351877027
92-93	22.479259747286818	27.7621725692699	27.255877458200317	22.50269022524297
94-95	22.46084632004912	27.82090046028161	27.286823853156434	22.43142936651283
96-97	22.43904786149102	27.785316144968185	27.382098595960148	22.393537397580644
98-99	22.70686356911018	28.30725146994894	27.496992125588633	21.488892835352242
100-101	26.843028870517404	35.238905217885616	28.127243918828142	9.79082199276884
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2378.0
1	2080.0
2	2449.0
3	3350.0
4	4384.0
5	6673.0
6	8749.0
7	10261.0
8	11382.5
9	11525.5
10	10791.0
11	9946.0
12	10706.5
13	13702.0
14	20476.5
15	29472.5
16	36686.5
17	41296.0
18	43220.0
19	42725.0
20	41357.5
21	40535.0
22	41569.0
23	46560.5
24	55738.0
25	68814.0
26	85465.0
27	103537.5
28	123542.0
29	145408.0
30	166484.0
31	187390.0
32	208818.5
33	229345.0
34	248520.0
35	266026.5
36	286337.0
37	305450.0
38	321004.0
39	337089.0
40	353928.0
41	369900.0
42	381644.0
43	391870.5
44	402043.5
45	411627.5
46	420130.5
47	427300.5
48	438688.5
49	441025.5
50	431409.5
51	422661.0
52	407406.0
53	385075.5
54	360007.5
55	333312.0
56	307225.0
57	281082.0
58	251480.0
59	219257.5
60	186928.5
61	154906.5
62	125119.5
63	99169.5
64	76346.0
65	57993.5
66	44360.5
67	34275.0
68	26243.5
69	19395.0
70	14306.5
71	10682.5
72	7808.5
73	5570.0
74	3947.0
75	2809.0
76	2062.0
77	1416.0
78	944.5
79	639.0
80	445.5
81	335.5
82	261.5
83	187.0
84	112.5
85	75.0
86	60.5
87	47.5
88	34.0
89	20.5
90	11.0
91	5.0
92	4.0
93	3.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.002182016201944651
3	0.0020660636501504907
4	6.219273232595864E-4
5	4.2164564288785523E-5
6	3.4785765538248057E-4
7	0.0
8	2.1082282144392762E-5
9	3.9002221967126605E-4
10-11	6.588213170122737E-4
12-13	1.633876866190439E-4
14-15	2.635285268049095E-5
16-17	4.321867839600516E-4
18-19	0.0
20-21	3.6895833364079864E-5
22-23	5.27140431317898E-6
24-25	0.0
26-27	8.44044032300088E-5
28-29	2.745715535316581E-4
30-31	6.349457975874282E-4
32-33	3.0269342986386123E-4
34-35	9.551312934681452E-4
36-37	1.450927145132642E-4
38-39	1.0312305457000671E-4
40-41	3.5092990942718367E-4
42-43	0.00131913105844194
44-45	3.8718474604328054E-4
46-47	1.6502962964733397E-4
48-49	4.6159833616879725E-4
50-51	0.0
52-53	4.2599434031019367E-4
54-55	6.225882562254785E-4
56-57	6.490597974545209E-4
58-59	6.827974593291078E-4
60-61	7.801164420524201E-4
62-63	5.698715015746816E-5
64-65	9.060699748202512E-4
66-67	0.0026058715314478407
68-69	0.001500257167976493
70-71	2.89903047656353E-4
72-73	0.0
74-75	1.0409000148918096E-4
76-77	1.4082600932465339E-5
78-79	0.0011792121619225533
80-81	2.2504022594038687E-4
82-83	1.253623617502031E-4
84-85	8.684614770702961E-4
86-87	2.432706584492879E-4
88-89	4.635953628410236E-5
90-91	3.929446319794531E-5
92-93	1.1199259952902313E-4
94-95	6.274490886179758E-4
96-97	3.32982979741482E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	330.0
20-21	821.0
22-23	1863.0
24-25	4089.0
26-27	7119.0
28-29	15417.0
30-31	32107.0
32-33	41656.0
34-35	58104.0
36-37	87680.0
38-39	95159.0
40-41	96269.0
42-43	105626.0
44-45	122562.0
46-47	123078.0
48-49	115515.0
50-51	105063.0
52-53	95720.0
54-55	107389.0
56-57	113859.0
58-59	115991.0
60-61	117816.0
62-63	113681.0
64-65	120194.0
66-67	127485.0
68-69	117691.0
70-71	110719.0
72-73	102707.0
74-75	104727.0
76-77	103062.0
78-79	108223.0
80-81	108782.0
82-83	102587.0
84-85	100849.0
86-87	104676.0
88-89	108203.0
90-91	111574.0
92-93	113064.0
94-95	122235.0
96-97	181493.0
98-99	726925.0
100-101	5134529.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.84216718333893
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.86793916890682	78.91168764911677
2	6.576375030034579	11.42213319637197
3	1.4372755642058368	3.7444837450587314
4	0.5189217223451983	1.8025714786787157
5	0.23328774441406702	1.0129606651115224
6	0.12264787042205344	0.6390604120723473
7	0.07197360362190594	0.4375240602964646
8	0.044397257994517686	0.30844432809933886
9	0.03150179688221544	0.24621158802788443
>10	0.09317108159812118	1.2791954826866858
>50	0.002138546272416166	0.1266301160830079
>100	3.5181316375588816E-4	0.05766876498172186
>500	1.880013853296035E-5	0.011428513414844925
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.1082282144392762E-5	0.0	1.0541141072196381E-5	0.0
3	0.0	2.1082282144392762E-5	0.0	1.0541141072196381E-5	0.0
4	0.0	5.27057053609819E-5	0.0	1.0541141072196381E-5	1.0541141072196381E-5
5	0.0	6.324684643317828E-5	0.0	2.1082282144392762E-5	1.0541141072196381E-5
6	8.432912857757105E-5	6.324684643317828E-5	0.0	2.1082282144392762E-5	2.1082282144392762E-5
7	8.432912857757105E-5	6.324684643317828E-5	0.0	2.1082282144392762E-5	2.1082282144392762E-5
8	9.487026964976743E-5	7.378798750537466E-5	0.0	2.1082282144392762E-5	3.162342321658914E-5
9	9.487026964976743E-5	2.0028168037173122E-4	0.0	5.27057053609819E-5	4.2164564288785523E-5
10-11	1.3703483393855294E-4	2.0028168037173122E-4	0.0	7.378798750537466E-5	4.2164564288785523E-5
12-13	1.7392882769124029E-4	2.0028168037173122E-4	0.0	8.432912857757105E-5	4.2164564288785523E-5
14-15	2.0028168037173125E-4	2.4244624466051676E-4	0.0	8.432912857757105E-5	4.7435134824883716E-5
16-17	2.635285268049095E-4	2.4244624466051676E-4	0.0	1.2122312233025836E-4	5.27057053609819E-5
18-19	2.740696678771059E-4	2.687990973410077E-4	0.0	1.581171160829457E-4	5.27057053609819E-5
20-21	2.740696678771059E-4	3.0569309109369503E-4	0.0	2.5825795626881133E-4	7.378798750537466E-5
22-23	3.0042252055759687E-4	3.425870848463824E-4	0.0	4.005633607434625E-4	1.10681981258062E-4
24-25	3.5839879645467696E-4	3.6893993752687334E-4	0.0	5.692216178986046E-4	1.3176426340245475E-4
26-27	3.8475164913516794E-4	3.794810785990697E-4	0.0	9.434321259615761E-4	1.3703483393855294E-4
28-29	3.900222196712661E-4	3.794810785990697E-4	0.0	0.003072742622545245	1.3703483393855294E-4
30-31	3.900222196712661E-4	4.058339312795606E-4	0.0	0.009813802338214831	1.4230540447465113E-4
32-33	4.216456428878552E-4	4.111045018156588E-4	0.0	0.022083690546251415	1.4757597501074933E-4
34-35	4.585396366405426E-4	4.954336303932299E-4	0.0	0.03745794480004984	1.4757597501074933E-4
36-37	4.848924893210335E-4	5.955744705790955E-4	0.0	0.05973137588560079	1.4757597501074933E-4
38-39	4.848924893210335E-4	6.324684643317828E-4	0.0	0.09650414651595787	1.4757597501074933E-4
40-41	5.165159125376227E-4	7.062564518371575E-4	0.0	0.17346501748406362	1.4757597501074933E-4
42-43	5.165159125376227E-4	7.273387339815502E-4	0.0	0.2056049566131904	1.5284654554684752E-4
44-45	5.217864830737208E-4	8.063972920230231E-4	0.0	0.2474901806635627	1.7392882769124029E-4
46-47	5.323276241459172E-4	8.538324268479069E-4	0.0	0.294097835914279	1.8974053929953486E-4
48-49	5.375981946820154E-4	8.538324268479069E-4	0.0	0.330918041679461	2.21363962516124E-4
50-51	5.428687652181137E-4	9.434321259615761E-4	0.0	0.36537176127393484	2.21363962516124E-4
52-53	5.586804768264082E-4	9.487026964976743E-4	0.0	0.41538420509097057	2.3190510358832037E-4
54-55	5.586804768264082E-4	9.539732670337724E-4	0.0	0.5188771281377946	2.4244624466051676E-4
56-57	5.586804768264082E-4	9.69784978642067E-4	0.0	0.6272611406421178	2.4244624466051676E-4
58-59	5.586804768264082E-4	9.855966902503617E-4	0.0	0.7235228409134151	2.4771681519661495E-4
60-61	5.903039000429973E-4	0.0010119495429308525	0.0	0.7824530900775291	2.529873857327131E-4
62-63	6.219273232595865E-4	0.0010435729661474416	0.0	0.8171966910514883	2.740696678771059E-4
64-65	6.219273232595865E-4	0.001138443235797209	0.0	0.8401131317424433	2.8461080894930227E-4
66-67	6.219273232595865E-4	0.0011542549474055038	0.0	0.8508229310717947	3.0569309109369503E-4
68-69	6.324684643317828E-4	0.001185878370622093	0.0	0.8615854361065073	3.0569309109369503E-4
70-71	6.482801759400774E-4	0.001191148941158191	0.0	0.8711989567643503	3.162342321658914E-4
72-73	6.746330286205684E-4	0.001191148941158191	0.0	0.8777555465112565	3.6893993752687334E-4
74-75	6.746330286205684E-4	0.0012069606527664856	0.0	0.8828679999312719	3.6893993752687334E-4
76-77	6.799035991566665E-4	0.001238584075983075	0.0	0.887279467469986	3.6893993752687334E-4
78-79	7.062564518371575E-4	0.001243854646519173	0.0	0.8916224175917309	3.6893993752687334E-4
80-81	7.062564518371575E-4	0.0012543957875913695	0.0	0.8933353530159628	3.847516491351679E-4
82-83	7.22068163445452E-4	0.001270207499199664	0.0	0.893888762922253	4.1637507235175704E-4
84-85	7.273387339815502E-4	0.0012754780697357621	0.0	0.8944421728285434	4.216456428878552E-4
86-87	7.273387339815502E-4	0.0012860192108079583	0.0	0.8946055605151624	4.216456428878552E-4
88-89	7.273387339815502E-4	0.0012965603518801548	0.0	0.8946688073615956	4.321867839600516E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9025	0.0	43.468754	1
GTATCAA	15470	0.0	32.893345	1
TCAACGC	17780	0.0	27.545692	4
ATCAACG	18110	0.0	27.111029	3
CAACGCA	18570	0.0	26.417868	5
TATCAAC	19180	0.0	25.959072	2
AACGCAG	19100	0.0	25.727333	6
GTACATG	23295	0.0	21.861609	1
ACGCAGA	22545	0.0	21.814066	7
TACATGG	23105	0.0	21.707424	2
CGCAGAG	22860	0.0	21.531242	8
ACATGGG	23730	0.0	20.348024	3
CTTATAC	16155	0.0	19.909739	1
CATGGGG	18350	0.0	18.235987	4
TATACAC	19595	0.0	18.009956	3
GCAGAGT	28380	0.0	17.271801	9
GAGTACT	16140	0.0	17.05915	12-13
TTATACA	19650	0.0	17.05056	2
AGAGTAC	24495	0.0	16.33049	10-11
CAGAGTA	27345	0.0	15.994779	10-11
>>END_MODULE