FastQCFastQC Report
Fri 27 May 2016
SRR2139771_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139771_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10467697
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102100.044.175591
GTATCAA175650.033.447021
TCAACGC197950.028.7822424
ATCAACG201750.028.2405263
CAACGCA205150.027.7920555
AACGCAG212850.026.8060266
TATCAAC216800.026.585122
ACGCAGA256000.022.3361647
CGCAGAG260400.021.9587488
GTACATG271350.021.939571
TACATGG267500.021.6542572
ACATGGG270500.020.9562593
CATGGGG204450.019.0956734
GCAGAGT316500.018.0535099
GTGGTAT57400.017.3817791
GAGTACT180100.017.3052612-13
AGAGTAC279200.016.50815210-11
CAGAGTA307000.016.4232910-11
TATCGCG3951.6007107E-1015.6554757
TGGTATC53900.015.6038662
Produced by FastQC (version 0.11.5)