##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139771_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10467697
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.511520155770654	34.0	31.0	34.0	31.0	34.0
2	32.698989567619314	34.0	31.0	34.0	31.0	34.0
3	32.793296749036585	34.0	31.0	34.0	31.0	34.0
4	36.20706789659655	37.0	37.0	37.0	35.0	37.0
5	36.05422596775585	37.0	35.0	37.0	35.0	37.0
6	36.078593600865595	37.0	35.0	37.0	35.0	37.0
7	36.09730717272386	37.0	35.0	37.0	35.0	37.0
8	36.09290878404295	37.0	35.0	37.0	35.0	37.0
9	37.967203578781465	39.0	38.0	39.0	35.0	39.0
10-11	37.84775161145761	39.0	38.0	39.0	35.0	39.0
12-13	37.87820878842787	39.0	38.0	39.0	35.0	39.0
14-15	39.21224300818031	40.0	39.0	41.0	36.0	41.0
16-17	39.182231296912775	40.0	39.0	41.0	36.0	41.0
18-19	39.20946918887698	40.0	39.0	41.0	36.0	41.0
20-21	39.24945877550287	40.0	39.0	41.0	36.0	41.0
22-23	39.21307859846359	40.0	39.0	41.0	36.0	41.0
24-25	39.20533115847468	40.0	39.0	41.0	36.0	41.0
26-27	39.10881950166389	40.0	39.0	41.0	36.0	41.0
28-29	39.03464823963557	40.0	39.0	41.0	36.0	41.0
30-31	38.92713526821431	40.0	38.0	41.0	35.0	41.0
32-33	38.86965911283836	40.0	38.0	41.0	35.0	41.0
34-35	38.812188666432306	40.0	38.0	41.0	35.0	41.0
36-37	38.750850635578	40.0	38.0	41.0	35.0	41.0
38-39	38.669912026611186	40.0	38.0	41.0	35.0	41.0
40-41	38.65339064015435	40.0	38.0	41.0	35.0	41.0
42-43	38.600844532841236	40.0	38.0	41.0	35.0	41.0
44-45	38.575446468730185	40.0	38.0	41.0	35.0	41.0
46-47	38.47479041894379	40.0	38.0	41.0	34.0	41.0
48-49	38.464437513153094	40.0	38.0	41.0	34.0	41.0
50-51	38.39866480059342	40.0	38.0	41.0	34.0	41.0
52-53	38.23379939665603	40.0	37.5	41.0	34.0	41.0
54-55	38.09454937717352	40.0	37.0	41.0	33.5	41.0
56-57	37.90641615095467	40.0	37.0	41.0	33.0	41.0
58-59	37.69216207985126	39.0	36.5	41.0	33.0	41.0
60-61	37.53179661067812	39.0	36.0	41.0	33.0	41.0
62-63	37.363923555482344	39.0	35.5	40.5	33.0	41.0
64-65	37.09849696307549	39.0	35.0	40.0	32.0	41.0
66-67	36.89785242697799	38.0	35.0	40.0	32.0	41.0
68-69	36.61688250492832	37.5	35.0	40.0	32.0	41.0
70-71	36.28151947458626	37.0	35.0	39.5	31.5	41.0
72-73	36.02175782136218	36.5	35.0	39.0	31.0	41.0
74-75	35.66943230519228	36.0	34.5	39.0	31.0	40.5
76-77	33.659395100984966	34.5	32.0	36.5	28.5	39.0
78-79	34.80047881452984	35.0	34.0	37.0	30.5	39.0
80-81	34.77038470448973	35.0	34.0	37.0	31.0	39.0
82-83	34.52897648842292	35.0	34.0	36.0	31.0	38.5
84-85	34.25434879505736	35.0	34.0	36.0	31.0	37.0
86-87	34.03544293053141	35.0	34.0	36.0	30.5	37.0
88-89	33.82872438362051	35.0	34.0	35.0	30.0	36.5
90-91	33.77721742260716	35.0	34.0	35.0	30.0	36.0
92-93	33.69741007779183	35.0	34.0	35.0	30.0	36.0
94-95	33.61675621473945	35.0	34.0	35.0	30.0	36.0
96-97	33.618543958114984	35.0	34.0	35.0	30.5	35.5
98-99	33.676558227452794	35.0	34.0	35.0	31.0	35.0
100-101	32.447067894572605	34.0	31.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	6.0
19	20.0
20	55.0
21	217.0
22	734.0
23	2545.0
24	6402.0
25	14200.0
26	26554.0
27	43717.0
28	64864.0
29	91182.0
30	122245.0
31	162158.0
32	216128.0
33	297117.0
34	440067.0
35	696834.0
36	1280260.0
37	2479211.0
38	3077246.0
39	1417351.0
40	28583.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.27869797912568	20.018109045380278	13.067697698930338	24.6354952765637
2	15.186468774716472	21.508803040431097	39.95952983067626	23.345198354176176
3	18.444643028080733	25.89376488780184	30.414029685514983	25.24756239860245
4	12.689277509060549	17.667021761271148	38.500298969213006	31.1434017604553
5	13.111394034428011	37.83222804404828	35.8750640183796	13.18131390314412
6	30.476546857325026	37.772208666713354	18.543611327040747	13.20763314892088
7	26.678810057264744	32.33286175555139	23.29526733530785	17.693060851876012
8	24.7978184308962	36.36432853651162	21.45380621044079	17.38404682215139
9	25.7107297268147	16.190308872367524	21.086963395915628	37.011998004902146
10-11	24.244848449993626	26.55145488474228	29.21109183001477	19.992604835249324
12-13	24.858645904863007	24.11017566063324	29.22988026768863	21.801298166815123
14-15	21.965247457307814	25.608896018125098	26.488440219572706	25.937416304994382
16-17	21.893956321997425	28.772946055031078	27.218719313190576	22.11437830978093
18-19	21.922950196208394	28.067950381062808	28.64055961879676	21.36853980393204
20-21	22.54049041727428	27.33774312211288	28.515110295354006	21.606656165258837
22-23	22.20478814732602	27.325786487298892	28.401698857281428	22.06772650809366
24-25	22.030820430401484	27.40585257214983	28.552046074052388	22.011280923396292
26-27	22.025682291337002	27.578368918352215	28.451279601621675	21.944669188689105
28-29	21.901598719845175	27.600434105844734	28.437861065077026	22.060106109233065
30-31	22.123794115884714	27.476306549224038	28.403226077056743	21.9966732578345
32-33	21.90957531682514	27.662903227512874	28.359493895387956	22.068027560274032
34-35	22.073814862712567	27.69572772180986	28.39752074797773	21.832936667499848
36-37	22.110169372889448	27.603720285082773	28.25672712439396	22.029383217633818
38-39	21.941485011823318	27.69679753537886	28.374956763248075	21.986760689549747
40-41	22.159713473876785	27.55619537796293	28.28594042236298	21.998150725797306
42-43	22.1173135100424	27.64047077050033	28.139174011104114	22.103041708353157
44-45	22.1172810180295	27.7398691264685	28.0257255625533	22.117124292948702
46-47	22.270937880408816	27.64584781138022	27.765345790812475	22.317868517398495
48-49	22.12240903828642	27.7896251476945	27.79058981023843	22.29737600378065
50-51	22.06327921239269	27.906886064103563	27.785783851337953	22.244050872165793
52-53	22.264780364894037	27.813412882418408	27.70498653995862	22.21682021272893
54-55	22.19054609088986	27.69638374478064	27.720108659357805	22.39296150497169
56-57	22.237831044519904	27.85267527518036	27.612841399033478	22.29665228126626
58-59	22.157538270264602	27.87051026909562	27.736493864436966	22.235457596202806
60-61	22.267156577702245	27.8850874017328	27.62238307011336	22.225372950451597
62-63	22.18642833118401	28.010537601332295	27.59922522939734	22.203808838086353
64-65	22.157356399796253	27.966951061235623	27.576512510126488	22.299180028841636
66-67	22.178175724321385	27.993716848618234	27.435668167207332	22.39243925985305
68-69	22.178119923971973	27.953139041832426	27.472855218879705	22.395885815315896
70-71	22.194049304990617	27.735141089024562	27.52217405502495	22.548635550959872
72-73	22.206956483583884	27.789909335375214	27.47482770442455	22.528306476616358
74-75	22.08940624907258	27.856460068761074	27.469005795387748	22.585127886778594
76-77	22.27745176181688	27.76425767781101	27.37952074560312	22.578769814768993
78-79	22.250642290760286	27.791616569890632	27.323198903515898	22.63454223583318
80-81	22.24007739097496	27.832763083262453	27.33243078659119	22.594728739171398
82-83	22.27032257472901	27.82100323030648	27.354571307940255	22.554102887024257
84-85	22.361268355704997	27.81785513891632	27.33188639201064	22.48899011336804
86-87	22.276752875630585	27.8331797790346	27.394206469050292	22.495860876284528
88-89	22.26287727193361	27.80263847480933	27.401862442492053	22.532621810765004
90-91	22.223872854986354	27.825908797819338	27.451138262801898	22.499080084392407
92-93	22.234068332532317	27.745907390296015	27.51534950724532	22.50467476992635
94-95	22.182491397339742	27.76443280863971	27.561311191902533	22.49176460211801
96-97	22.166801255733724	27.802466709736134	27.578757757261137	22.45197427726901
98-99	22.432252926832284	28.416005639265073	27.61728829445123	21.534453139451408
100-101	26.553228630685776	35.41262721015424	28.26746378717258	9.766680371987395
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2680.0
1	2335.0
2	2748.5
3	3712.0
4	4772.0
5	7277.5
6	9655.0
7	11468.5
8	12749.0
9	12880.5
10	11998.5
11	10968.5
12	11689.5
13	14962.5
14	22347.0
15	31972.0
16	39872.0
17	44972.0
18	47012.0
19	46509.0
20	45228.5
21	44656.5
22	46326.0
23	52712.0
24	63789.0
25	78945.5
26	98950.5
27	120910.5
28	144547.0
29	170686.0
30	194566.0
31	217558.5
32	240936.5
33	263038.5
34	283537.5
35	302542.0
36	324247.0
37	343478.5
38	359306.5
39	374668.0
40	389513.5
41	405165.0
42	416562.0
43	426327.0
44	437348.0
45	446059.5
46	453160.5
47	458846.0
48	458059.0
49	453071.0
50	447424.0
51	435033.5
52	415821.5
53	395199.5
54	373732.0
55	349567.5
56	324455.0
57	297947.5
58	268464.5
59	235279.5
60	199794.0
61	165400.0
62	133695.0
63	105669.0
64	80907.0
65	61356.0
66	46790.0
67	36192.0
68	27972.0
69	20486.5
70	14900.0
71	10993.5
72	7997.5
73	5725.0
74	3947.0
75	2759.0
76	1990.5
77	1390.0
78	924.5
79	593.5
80	410.5
81	309.0
82	241.5
83	175.5
84	120.5
85	82.5
86	59.0
87	42.0
88	25.0
89	16.0
90	11.5
91	7.0
92	5.0
93	5.0
94	4.0
95	2.0
96	1.0
97	0.5
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.002235448733374686
3	0.0022450019330899624
4	6.114047817776918E-4
5	0.0
6	3.6302158918050456E-4
7	0.0
8	1.910639943055287E-5
9	3.8212798861105745E-4
10-11	4.442237867603543E-4
12-13	1.2419159629859365E-4
14-15	2.388299928819109E-5
16-17	4.585535863332689E-4
18-19	0.0
20-21	6.209889784487686E-5
22-23	0.0
24-25	0.0
26-27	9.561315376928087E-5
28-29	2.9186556786776365E-4
30-31	6.75889733335615E-4
32-33	4.087646252977309E-4
34-35	8.887167697811568E-4
36-37	1.361016407052787E-4
38-39	1.1770093007765368E-4
40-41	2.7720720669435604E-4
42-43	0.0012896943424408416
44-45	4.6006183028774085E-4
46-47	1.3827209151441073E-4
48-49	4.512110894616227E-4
50-51	0.0
52-53	4.510006138383649E-4
54-55	7.670442598484192E-4
56-57	6.459654814502869E-4
58-59	7.308833208524474E-4
60-61	7.400694229634194E-4
62-63	5.633872645182081E-5
64-65	7.530098574124494E-4
66-67	0.0025792618441756883
68-69	0.0013959157148437454
70-71	2.4383208889761136E-4
72-73	0.0
74-75	1.5266102517007813E-4
76-77	6.189262063568054E-6
78-79	0.001129400001229791
80-81	2.4187803783698144E-4
82-83	8.395095946582909E-5
84-85	9.10278557681307E-4
86-87	1.99291359782884E-4
88-89	3.372058502787141E-5
90-91	4.1113529804842295E-5
92-93	1.2541285214187037E-4
94-95	6.735461488651846E-4
96-97	2.1705964350413563E-5
98-99	2.263189130717353E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	371.0
20-21	906.0
22-23	1834.0
24-25	4384.0
26-27	7121.0
28-29	15272.0
30-31	31336.0
32-33	41568.0
34-35	57973.0
36-37	86568.0
38-39	95711.0
40-41	96822.0
42-43	107447.0
44-45	125202.0
46-47	124925.0
48-49	115855.0
50-51	106418.0
52-53	99779.0
54-55	108840.0
56-57	112888.0
58-59	111836.0
60-61	113638.0
62-63	108217.0
64-65	115678.0
66-67	122507.0
68-69	118385.0
70-71	113769.0
72-73	107361.0
74-75	109985.0
76-77	107970.0
78-79	113315.0
80-81	113978.0
82-83	108226.0
84-85	107573.0
86-87	111559.0
88-89	115834.0
90-91	120410.0
92-93	121958.0
94-95	134030.0
96-97	199758.0
98-99	825433.0
100-101	5925057.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.83655576706649
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.82330958385889	78.86783387628307
2	6.571008761082335	11.412075374552174
3	1.5060145125893998	3.9233133962544255
4	0.5238692100622941	1.8196399149689388
5	0.21806766779063516	0.9468122597547809
6	0.12165083628914633	0.6338243777719638
7	0.06877435674030921	0.418048978509679
8	0.04493836091982361	0.31218339872758594
9	0.02727621594448222	0.21317153822798204
>10	0.09259192162108093	1.2522260181870217
>50	0.002028196865743715	0.11870171615626422
>100	4.3630750542970377E-4	0.059389641683431416
>500	3.4068730491721217E-5	0.022779508922740853
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.553199715276436E-6
2	0.0	0.0	0.0	0.0	9.553199715276436E-6
3	0.0	0.0	9.553199715276436E-6	0.0	9.553199715276436E-6
4	0.0	1.9106399430552872E-5	9.553199715276436E-6	9.553199715276436E-6	9.553199715276436E-6
5	0.0	1.9106399430552872E-5	9.553199715276436E-6	9.553199715276436E-6	9.553199715276436E-6
6	0.0	1.9106399430552872E-5	9.553199715276436E-6	9.553199715276436E-6	2.865959914582931E-5
7	0.0	1.9106399430552872E-5	1.9106399430552872E-5	9.553199715276436E-6	2.865959914582931E-5
8	0.0	2.865959914582931E-5	1.9106399430552872E-5	9.553199715276436E-6	2.865959914582931E-5
9	0.0	4.776599857638218E-5	1.9106399430552872E-5	9.553199715276436E-6	5.731919829165862E-5
10-11	0.0	4.776599857638218E-5	2.388299928819109E-5	3.8212798861105745E-5	5.731919829165862E-5
12-13	0.0	4.776599857638218E-5	2.865959914582931E-5	4.776599857638218E-5	6.687239800693505E-5
14-15	0.0	7.642559772221149E-5	2.865959914582931E-5	4.776599857638218E-5	9.075539729512614E-5
16-17	0.0	7.642559772221149E-5	2.865959914582931E-5	7.642559772221149E-5	1.0508519686804079E-4
18-19	0.0	9.553199715276436E-5	2.865959914582931E-5	1.0986179672567901E-4	1.0508519686804079E-4
20-21	0.0	1.1941499644095546E-4	3.8212798861105745E-5	1.8151079459025228E-4	1.0986179672567901E-4
22-23	0.0	1.4329799572914654E-4	3.8212798861105745E-5	2.722661918853784E-4	1.337447960138701E-4
24-25	0.0	1.5285119544442298E-4	3.8212798861105745E-5	3.439151897499517E-4	1.624043951596994E-4
26-27	0.0	1.6718099501733763E-4	4.2989398718743966E-5	4.7288338590618353E-4	1.624043951596994E-4
28-29	0.0	1.7195759487497584E-4	4.776599857638218E-5	0.0015858311527358884	1.624043951596994E-4
30-31	0.0	2.1972359345135803E-4	6.687239800693505E-5	0.0046619614610549	1.624043951596994E-4
32-33	0.0	2.1972359345135803E-4	7.642559772221149E-5	0.010006976701752067	1.7195759487497584E-4
34-35	0.0	2.5315979245482554E-4	7.642559772221149E-5	0.017472802279240602	1.7195759487497584E-4
36-37	0.0	2.961491911735695E-4	7.642559772221149E-5	0.028487641550954332	1.7673419473261405E-4
38-39	0.0	3.29585390177037E-4	8.597879743748792E-5	0.048597126951611225	1.8628739444789052E-4
40-41	0.0	3.8212798861105745E-4	9.553199715276436E-5	0.0926755904378967	2.0539379387844335E-4
42-43	0.0	4.0123438804161033E-4	9.553199715276436E-5	0.11030602051243937	2.1017039373608158E-4
44-45	0.0	4.967663851943747E-4	9.553199715276436E-5	0.1332432530288181	2.1494699359371982E-4
46-47	0.0	5.731919829165861E-4	9.553199715276436E-5	0.15933304145123803	2.2450019330899623E-4
48-49	0.0	5.731919829165861E-4	9.553199715276436E-5	0.18223683776861327	2.674895920277402E-4
50-51	0.0	6.639473802117123E-4	9.553199715276436E-5	0.2043477185096206	2.674895920277402E-4
52-53	0.0	6.687239800693505E-4	9.553199715276436E-5	0.23457881900861288	2.9137259131593125E-4
54-55	0.0	6.78277179784627E-4	9.553199715276436E-5	0.2953514989973439	3.2003219046176055E-4
56-57	0.0	6.878303794999034E-4	9.553199715276436E-5	0.3634180469686885	3.29585390177037E-4
58-59	0.0	6.878303794999034E-4	9.553199715276436E-5	0.43062003036580065	3.391385898923135E-4
60-61	0.0	6.926069793575416E-4	1.0030859701040258E-4	0.4741396316687424	3.439151897499517E-4
62-63	0.0	6.973835792151798E-4	1.0508519686804079E-4	0.49437808526555554	3.5346838946522815E-4
64-65	0.0	7.738091769373913E-4	1.2419159629859368E-4	0.5135131442952543	3.582449893228663E-4
66-67	0.0	7.738091769373913E-4	1.337447960138701E-4	0.5230950036096764	3.7257478889578103E-4
68-69	0.0	7.88138976510306E-4	1.337447960138701E-4	0.5308760847777692	3.8212798861105745E-4
70-71	0.0	7.929155763679442E-4	1.337447960138701E-4	0.5387622511427299	3.8212798861105745E-4
72-73	0.0	7.929155763679442E-4	1.337447960138701E-4	0.5450387033556665	4.4900038661799247E-4
74-75	0.0	8.024687760832207E-4	1.337447960138701E-4	0.5499729310086068	4.4900038661799247E-4
76-77	0.0	8.263517753714117E-4	1.3852139587150833E-4	0.5539757216893075	4.537769864756307E-4
78-79	0.0	8.311283752290499E-4	1.4329799572914654E-4	0.5575581715825363	4.5855358633326894E-4
80-81	0.0	8.311283752290499E-4	1.4329799572914654E-4	0.5592538645319978	4.728833859061836E-4
82-83	0.0	8.311283752290499E-4	1.4329799572914654E-4	0.5598891523130637	5.158727846249275E-4
84-85	0.0	8.311283752290499E-4	1.4329799572914654E-4	0.5604957804949837	5.206493844825658E-4
86-87	0.0	8.406815749443263E-4	1.4329799572914654E-4	0.5607919296861573	5.25425984340204E-4
88-89	0.0	8.884475735207086E-4	1.4329799572914654E-4	0.5609065680827406	5.445323837707568E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10210	0.0	44.17559	1
GTATCAA	17565	0.0	33.44702	1
TCAACGC	19795	0.0	28.782242	4
ATCAACG	20175	0.0	28.240526	3
CAACGCA	20515	0.0	27.792055	5
AACGCAG	21285	0.0	26.806026	6
TATCAAC	21680	0.0	26.58512	2
ACGCAGA	25600	0.0	22.336164	7
CGCAGAG	26040	0.0	21.958748	8
GTACATG	27135	0.0	21.93957	1
TACATGG	26750	0.0	21.654257	2
ACATGGG	27050	0.0	20.956259	3
CATGGGG	20445	0.0	19.095673	4
GCAGAGT	31650	0.0	18.053509	9
GTGGTAT	5740	0.0	17.381779	1
GAGTACT	18010	0.0	17.30526	12-13
AGAGTAC	27920	0.0	16.508152	10-11
CAGAGTA	30700	0.0	16.42329	10-11
TATCGCG	395	1.6007107E-10	15.655475	7
TGGTATC	5390	0.0	15.603866	2
>>END_MODULE