FastQCFastQC Report
Fri 27 May 2016
SRR2139770_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139770_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9575536
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108900.046.1498151
GTATCAA194750.034.87631
TCAACGC233450.028.3357224
ATCAACG234800.028.1900883
CAACGCA240000.027.6291685
TATCAAC243250.027.492462
AACGCAG247200.026.8732666
CTTATAC74000.024.360251
ACGCAGA291450.022.8620837
CGCAGAG294250.022.617198
GTACATG328750.022.3129181
TACATGG324600.022.1767182
ACATGGG334550.021.1319393
TATACAC107750.019.3811133
CATGGGG208800.018.943034
GCAGAGT353400.018.7861149
GTGGTAT53600.018.3923281
TGGTATC52350.017.8322732
GAGTACT189150.017.61341512-13
ACATCTC111100.017.0579348
Produced by FastQC (version 0.11.5)