FastQCFastQC Report
Fri 27 May 2016
SRR2139769_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139769_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12130240
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107150.041.46331
GTATCAA182750.033.1125561
TCAACGC204100.028.6103484
ATCAACG207400.028.194973
CAACGCA212600.027.4283925
AACGCAG221250.026.3560476
TATCAAC227700.026.1108572
GTACATG292850.022.165341
ACGCAGA263700.022.1285517
CGCAGAG270850.021.5443958
TACATGG293850.021.3415682
ACATGGG297600.020.5661033
CATGGGG217500.018.4788484
GCAGAGT338850.017.1247569
GAGTACT192450.016.01737612-13
AGAGTAC295850.016.00071110-11
CAGAGTA326550.015.53768310-11
GAGTACA208050.015.2475841
GTGGTAT65100.015.1379021
ATGGGGG134800.014.5605485
Produced by FastQC (version 0.11.5)