##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139769_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12130240
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.50630135924763	34.0	31.0	34.0	31.0	34.0
2	32.702512810958396	34.0	31.0	34.0	31.0	34.0
3	32.79970008837418	34.0	31.0	34.0	31.0	34.0
4	36.21370327380167	37.0	37.0	37.0	35.0	37.0
5	36.05611851043343	37.0	35.0	37.0	35.0	37.0
6	36.07826992705833	37.0	35.0	37.0	35.0	37.0
7	36.10019628630596	37.0	35.0	37.0	35.0	37.0
8	36.09743030640779	37.0	35.0	37.0	35.0	37.0
9	37.97706739520405	39.0	38.0	39.0	35.0	39.0
10-11	37.85967161408183	39.0	38.0	39.0	35.0	39.0
12-13	37.89138788680191	39.0	38.0	39.0	35.0	39.0
14-15	39.231953160036404	40.0	39.0	41.0	36.0	41.0
16-17	39.20428466378242	40.0	39.0	41.0	36.0	41.0
18-19	39.23009355132298	40.0	39.0	41.0	36.0	41.0
20-21	39.27050415746977	40.0	39.0	41.0	36.0	41.0
22-23	39.23598113997102	40.0	39.0	41.0	36.0	41.0
24-25	39.229918871826655	40.0	39.0	41.0	36.0	41.0
26-27	39.136080506824044	40.0	39.0	41.0	36.0	41.0
28-29	39.06500110955472	40.0	39.0	41.0	36.0	41.0
30-31	38.959486409956384	40.0	38.5	41.0	35.5	41.0
32-33	38.908367244043916	40.0	38.0	41.0	35.0	41.0
34-35	38.85862639257321	40.0	38.0	41.0	35.0	41.0
36-37	38.800365455803984	40.0	38.0	41.0	35.0	41.0
38-39	38.72723378549799	40.0	38.0	41.0	35.0	41.0
40-41	38.71173842203024	40.0	38.0	41.0	35.0	41.0
42-43	38.665470480525	40.0	38.0	41.0	35.0	41.0
44-45	38.64989302399957	40.0	38.0	41.0	35.0	41.0
46-47	38.556084749801634	40.0	38.0	41.0	34.5	41.0
48-49	38.55329624231519	40.0	38.0	41.0	34.0	41.0
50-51	38.498427923301136	40.0	38.0	41.0	34.0	41.0
52-53	38.34017792989564	40.0	38.0	41.0	34.0	41.0
54-55	38.21052928553826	40.0	37.5	41.0	34.0	41.0
56-57	38.039531498181745	40.0	37.0	41.0	34.0	41.0
58-59	37.83623145475465	39.5	36.5	41.0	33.0	41.0
60-61	37.69211195104635	39.0	36.0	41.0	33.0	41.0
62-63	37.533903824293304	39.0	36.0	41.0	33.0	41.0
64-65	37.272221131952605	39.0	35.0	40.0	33.0	41.0
66-67	37.07220408784876	38.5	35.0	40.0	32.0	41.0
68-69	36.794242922800194	38.0	35.0	40.0	32.0	41.0
70-71	36.46076198168278	37.0	35.0	39.5	32.0	41.0
72-73	36.196536156815654	37.0	35.0	39.0	32.0	41.0
74-75	35.83360389178954	36.0	35.0	39.0	31.5	40.5
76-77	33.811853584609885	34.5	32.0	36.5	29.0	39.0
78-79	34.93833515753142	35.0	34.0	37.0	31.0	39.0
80-81	34.89474748591719	35.0	34.0	37.0	31.0	39.0
82-83	34.63930468288743	35.0	34.0	36.5	31.0	39.0
84-85	34.35770230866129	35.0	34.0	36.0	31.0	37.0
86-87	34.13312886490698	35.0	34.0	36.0	31.0	37.0
88-89	33.920894666083434	35.0	34.0	35.0	30.5	37.0
90-91	33.859323051574634	35.0	34.0	35.0	31.0	36.0
92-93	33.76968876025596	35.0	34.0	35.0	31.0	36.0
94-95	33.679395886910164	35.0	34.0	35.0	31.0	36.0
96-97	33.677065212850344	35.0	34.0	35.0	31.0	36.0
98-99	33.72789178692837	35.0	34.0	35.0	31.0	35.0
100-101	32.50066304040203	34.0	31.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	5.0
19	20.0
20	69.0
21	200.0
22	789.0
23	2651.0
24	6737.0
25	14955.0
26	27889.0
27	45756.0
28	69999.0
29	99158.0
30	133977.0
31	178389.0
32	239878.0
33	329598.0
34	489183.0
35	771067.0
36	1416222.0
37	2803662.0
38	3697752.0
39	1766308.0
40	35974.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.06521058115915	20.206986836204393	13.057128300841534	24.67067428179492
2	15.08614506639899	21.778657286510374	40.04182521291831	23.09337243417232
3	18.22801636345859	26.143240114513212	30.57657926814891	25.052164253879283
4	12.564373619711459	17.85682517209373	39.04217115631505	30.536630051879765
5	13.012398189351421	37.990125338832975	36.06768176620428	12.929794705611325
6	30.065130911028977	38.14590626606272	18.84899529561998	12.939967527288331
7	26.356988814730787	32.50967829160841	23.771096037671143	17.36223685598966
8	24.5078826166284	36.46585276659086	21.76917250414584	17.257092112634904
9	25.695914548404808	16.18560930913772	21.41890781751216	36.69956832494531
10-11	23.980301843242845	26.662881614071292	29.601133801045915	19.755682741639944
12-13	24.65453652718424	24.297247223588414	29.4949409846115	21.553275264615845
14-15	21.822692560255735	25.794635231937583	26.768078822542236	25.61459338526445
16-17	21.688997371147284	28.82146632991477	27.5519279510435	21.937608347894447
18-19	21.722467980847863	28.310433264304745	28.81773567546891	21.149363079378478
20-21	22.370942175357335	27.60131994231312	28.66126237579888	21.366475506530666
22-23	22.1071386624378	27.512709750197175	28.52831895885895	21.851832628506077
24-25	21.904998283154164	27.58335196840591	28.59306432219516	21.91858542624476
26-27	21.856692387746108	27.796591388708507	28.50152528241146	21.84519094113392
28-29	21.843282466639636	27.87600242125352	28.487286354518755	21.793428757588096
30-31	22.06740992842187	27.742544642977208	28.394354763465714	21.795690665135208
32-33	21.817975758390734	27.89382274313174	28.38422730166361	21.903974196813913
34-35	21.99102152250953	27.91791022816419	28.347688420827684	21.7433798284986
36-37	22.03430400361654	27.826110830764456	28.30502009624435	21.834565069374655
38-39	21.824330328810778	27.979768536163103	28.385920265939923	21.8099808690862
40-41	22.058748122539395	27.824621065814426	28.294648647854743	21.821982163791436
42-43	21.95136765552619	27.919027367003597	28.100414421597193	22.029190555873022
44-45	22.011978482555545	27.968083465652825	28.035345263747676	21.984592788043958
46-47	22.105785893046995	27.927206951985102	27.82885050270108	22.13815665226683
48-49	21.997241455897072	28.005711155726342	27.87135602283486	22.12569136554173
50-51	22.01375363159116	28.108019736048607	27.81330560307741	22.06492102928282
52-53	22.198524628921238	27.99318199236994	27.67792657047326	22.13036680823556
54-55	22.12378795225563	27.929036066000425	27.748784226055722	22.198391755688224
56-57	22.110166471734576	28.060942522860728	27.704307441679415	22.124583563725285
58-59	22.10843029683198	28.044418587833096	27.698581854708625	22.148569260626296
60-61	22.166150063136808	28.043502288281395	27.673547975660785	22.116799672921008
62-63	22.17757184887079	28.056872751753893	27.67740430881415	22.088151090561162
64-65	22.1365572157023	28.01539169400837	27.63591468214402	22.212136408145305
66-67	22.148148811609715	28.06483309487733	27.546055155346867	22.240962938166085
68-69	22.128937953541246	28.042990091477936	27.609590672417582	22.218481282563236
70-71	22.13066649078914	27.89165783655659	27.661525793778747	22.316149878875528
72-73	22.195048519792635	27.90174562025044	27.586226315266316	22.316979544690607
74-75	22.06838200643589	28.021354229780133	27.5841122376163	22.326151526167685
76-77	22.176576592717506	27.953560609458005	27.535637994567935	22.334224803256554
78-79	22.16876249204145	27.94087262950489	27.471639629765598	22.41872524868806
80-81	22.17103158896787	27.93100179277864	27.49825940407059	22.399707214182897
82-83	22.221120594878766	27.928318707897738	27.579255773310983	22.271304923912517
84-85	22.2548153547823	27.908542084901367	27.532567620384228	22.304074939932107
86-87	22.126737640485512	27.938284580095356	27.577824958794366	22.357152820624766
88-89	22.22380450928317	27.880488052992654	27.529526959951404	22.366180477772772
90-91	22.225352742124933	27.923141175544767	27.56526202937356	22.28624405295674
92-93	22.267129053775633	27.878550311035283	27.580372271326265	22.273948363862818
94-95	22.175063984896624	27.90165879131229	27.617829158502726	22.30544806528836
96-97	22.164214340285938	27.904222525758637	27.658838019659765	22.272725114295664
98-99	22.413611007416577	28.46119666532646	27.708529300396663	21.4166630268603
100-101	26.48791085696422	35.52879592402234	28.30749917989336	9.675794039120083
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2287.0
1	2066.5
2	2653.0
3	3701.5
4	5062.0
5	8051.0
6	10512.5
7	12032.5
8	13024.5
9	12932.0
10	12006.0
11	11079.0
12	11956.0
13	15537.0
14	23408.5
15	33660.0
16	42194.5
17	47852.5
18	50368.0
19	50589.0
20	50227.5
21	50634.0
22	53656.0
23	61554.5
24	74846.5
25	93540.0
26	118209.0
27	145576.0
28	174757.5
29	206177.5
30	235282.0
31	263683.5
32	292627.5
33	320506.0
34	347494.0
35	373889.0
36	402867.5
37	427997.0
38	449813.5
39	470286.5
40	487810.0
41	504756.0
42	518098.5
43	529386.0
44	538905.5
45	544594.5
46	546302.5
47	544150.5
48	539098.0
49	529781.0
50	516715.0
51	498415.5
52	475032.5
53	448277.5
54	418973.5
55	389285.0
56	358513.5
57	326005.0
58	291628.5
59	253947.5
60	215267.0
61	178666.0
62	143945.5
63	113457.5
64	87240.0
65	66317.0
66	50550.0
67	38854.5
68	29807.5
69	22120.0
70	16302.5
71	11918.0
72	8619.5
73	6337.5
74	4553.0
75	3168.0
76	2169.5
77	1426.0
78	961.0
79	672.0
80	455.5
81	305.5
82	223.5
83	170.0
84	128.0
85	89.0
86	59.0
87	44.0
88	35.5
89	25.0
90	15.5
91	11.0
92	8.5
93	5.0
94	1.5
95	0.0
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0019455509536497216
3	0.0023577439523043238
4	6.347772179280871E-4
5	8.243859973092041E-6
6	4.5341229852006223E-4
7	0.0
8	3.2975439892368166E-5
9	4.204368586276941E-4
10-11	5.729482681298969E-4
12-13	1.1541403962328857E-4
14-15	3.709736987891419E-5
16-17	3.586079088295038E-4
18-19	0.0
20-21	7.419847101684057E-5
22-23	1.6490238294249753E-5
24-25	0.0
26-27	7.838138965088435E-5
28-29	2.147082412290032E-4
30-31	6.537339608131176E-4
32-33	3.197729661774888E-4
34-35	8.639899506699303E-4
36-37	1.2613481389817287E-4
38-39	1.486768335283761E-4
40-41	3.1349961553351946E-4
42-43	0.0013067998265299207
44-45	3.6930323383014586E-4
46-47	1.694567799813749E-4
48-49	3.165117296534052E-4
50-51	0.0
52-53	4.312006796835488E-4
54-55	6.290490041849128E-4
56-57	6.756479309019619E-4
58-59	6.464647088402554E-4
60-61	7.04232102381263E-4
62-63	5.450065410694138E-5
64-65	7.629133754525092E-4
66-67	0.0023612642738170906
68-69	0.00140950148565587
70-71	3.038383320239473E-4
72-73	0.0
74-75	1.0232785640536565E-4
76-77	5.462326389514781E-6
78-79	9.532434107049235E-4
80-81	1.9128936854760563E-4
82-83	1.484910228038806E-4
84-85	7.416986393828187E-4
86-87	2.881504592324436E-4
88-89	4.181377649246211E-5
90-91	2.4288510587240323E-5
92-93	1.4824565169251752E-4
94-95	6.098552737989262E-4
96-97	1.92508728185312E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	446.0
20-21	1031.0
22-23	2205.0
24-25	4724.0
26-27	8547.0
28-29	19355.0
30-31	41642.0
32-33	55817.0
34-35	77367.0
36-37	115602.0
38-39	129787.0
40-41	124476.0
42-43	137692.0
44-45	159076.0
46-47	157078.0
48-49	146355.0
50-51	133382.0
52-53	129810.0
54-55	141211.0
56-57	144723.0
58-59	141571.0
60-61	134511.0
62-63	128982.0
64-65	134533.0
66-67	141000.0
68-69	140479.0
70-71	135483.0
72-73	127326.0
74-75	130384.0
76-77	130169.0
78-79	134924.0
80-81	133934.0
82-83	126381.0
84-85	125566.0
86-87	130525.0
88-89	135125.0
90-91	139859.0
92-93	139903.0
94-95	152369.0
96-97	224993.0
98-99	928738.0
100-101	6683159.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.43620439378364
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.33660428007275	79.86126000466575
2	6.263179595174982	10.952573024773898
3	1.3583776233292262	3.5631415055206874
4	0.48636896901021254	1.7010502634068305
5	0.21387146233190577	0.9350054447224957
6	0.11904425226478725	0.6245266543757448
7	0.06383704078313895	0.39071679820661875
8	0.03836416428700691	0.26835335279963446
9	0.023940720013861272	0.1883957119619689
>10	0.09382774670885916	1.2890997806710345
>50	0.0020756751245083575	0.12383954207212118
>100	4.653051915657762E-4	0.070189526830029
>500	2.8784463515386633E-5	0.016520179728835557
>1k	1.4381243739604536E-5	0.01532821026437465
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.243859973092041E-6
2	0.0	1.6487719946184083E-5	0.0	0.0	8.243859973092041E-6
3	0.0	1.6487719946184083E-5	0.0	0.0	8.243859973092041E-6
4	0.0	8.243859973092041E-5	0.0	2.4731579919276124E-5	8.243859973092041E-6
5	0.0	8.243859973092041E-5	0.0	3.2975439892368166E-5	8.243859973092041E-6
6	8.243859973092041E-6	8.243859973092041E-5	0.0	3.2975439892368166E-5	1.6487719946184083E-5
7	8.243859973092041E-6	8.243859973092041E-5	0.0	4.946315983855225E-5	1.6487719946184083E-5
8	8.243859973092041E-6	1.0717017965019654E-4	0.0	4.946315983855225E-5	2.4731579919276124E-5
9	8.243859973092041E-6	1.8960877938111695E-4	0.0	7.419473975782837E-5	3.2975439892368166E-5
10-11	8.243859973092041E-6	1.8960877938111695E-4	0.0	9.89263196771045E-5	4.121929986546021E-5
12-13	8.243859973092041E-6	1.8960877938111695E-4	0.0	1.1129210963674256E-4	4.121929986546021E-5
14-15	8.243859973092041E-6	2.2258421927348512E-4	0.0	1.2777982958292664E-4	5.770701981164429E-5
16-17	2.4731579919276124E-5	2.2258421927348512E-4	0.0	1.4426754952911073E-4	9.068245970401246E-5
18-19	2.4731579919276124E-5	2.390719392196692E-4	0.0	1.607552694752948E-4	9.89263196771045E-5
20-21	2.4731579919276124E-5	2.596815891523993E-4	0.0	1.97852639354209E-4	9.89263196771045E-5
22-23	2.8853509905822145E-5	3.0914474899095153E-4	0.0	2.3082807924657716E-4	1.0717017965019654E-4
24-25	3.2975439892368166E-5	3.338763289102277E-4	0.0	2.967789590313135E-4	1.1541403962328858E-4
26-27	3.2975439892368166E-5	3.4624211886986574E-4	0.0	3.9158334872187194E-4	1.1541403962328858E-4
28-29	3.2975439892368166E-5	3.4624211886986574E-4	0.0	9.109465270266705E-4	1.1541403962328858E-4
30-31	3.2975439892368166E-5	3.7097369878914187E-4	0.0	0.0026710106312818212	1.195359696098346E-4
32-33	3.2975439892368166E-5	3.7097369878914187E-4	0.0	0.006166407259872847	1.2365789959638062E-4
34-35	3.2975439892368166E-5	4.2868071860078616E-4	0.0	0.011442477642651754	1.2365789959638062E-4
36-37	3.2975439892368166E-5	4.8638773841243045E-4	0.0	0.01876714722874403	1.2365789959638062E-4
38-39	3.2975439892368166E-5	5.069973883451605E-4	0.0	0.029183264304745826	1.2777982958292664E-4
40-41	3.2975439892368166E-5	5.688263381433508E-4	0.0	0.039830209459994194	1.3602368955601868E-4
42-43	3.2975439892368166E-5	5.93557918062627E-4	0.0	0.04929416070910386	1.4426754952911073E-4
44-45	3.2975439892368166E-5	6.759965177935473E-4	0.0	0.060369786582953014	1.566333394887488E-4
46-47	3.2975439892368166E-5	7.337035376051916E-4	0.0	0.0719400440551877	1.6487719946184083E-4
48-49	3.2975439892368166E-5	7.337035376051916E-4	0.0	0.0834938138074762	1.7312105943493287E-4
50-51	3.2975439892368166E-5	8.202640673226581E-4	0.0	0.09434273353206532	1.772429894214789E-4
52-53	3.2975439892368166E-5	8.243859973092041E-4	0.0	0.10433429181945286	2.1021842931384706E-4
54-55	3.2975439892368166E-5	8.243859973092041E-4	0.0	0.11448248344632916	2.2258421927348512E-4
56-57	3.2975439892368166E-5	8.408737172553882E-4	0.0	0.12398353206531776	2.2670614926003114E-4
58-59	3.2975439892368166E-5	8.408737172553882E-4	0.0	0.13287041311631098	2.3082807924657716E-4
60-61	3.709736987891419E-5	8.491175772284802E-4	0.0	0.14079276255045242	2.3082807924657716E-4
62-63	4.121929986546021E-5	8.656052971746644E-4	0.0	0.14855023478513202	2.3082807924657716E-4
64-65	4.121929986546021E-5	9.604096868652229E-4	0.0	0.15674463159838553	2.3082807924657716E-4
66-67	4.121929986546021E-5	9.645316168517688E-4	0.0	0.16423417838393967	2.3082807924657716E-4
68-69	4.121929986546021E-5	9.810193367979529E-4	0.0	0.17171960323950722	2.3495000923312318E-4
70-71	4.121929986546021E-5	0.001001628986730683	0.0	0.17906076054554568	2.4731579919276124E-4
72-73	4.946315983855225E-5	0.001005750916717229	0.0	0.18498809586619885	2.6380351913894533E-4
74-75	4.946315983855225E-5	0.001013994776690321	0.0	0.18972419342074023	2.7204737911203737E-4
76-77	4.946315983855225E-5	0.0010346044266230513	0.0	0.19430365763579288	2.7204737911203737E-4
78-79	4.946315983855225E-5	0.0010469702165826892	0.0	0.19829780779275596	2.7204737911203737E-4
80-81	4.946315983855225E-5	0.0010469702165826892	0.0	0.1999218482074551	2.802912390851294E-4
82-83	4.946315983855225E-5	0.0010469702165826892	0.0	0.20059372279526208	3.0502281900440554E-4
84-85	4.946315983855225E-5	0.0010469702165826892	0.0	0.20134803598280002	3.0502281900440554E-4
86-87	4.946315983855225E-5	0.0010552140765557813	0.0	0.2016077175719524	3.0502281900440554E-4
88-89	4.946315983855225E-5	0.0011376526762867016	0.0	0.20171076582161607	3.0914474899095153E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10715	0.0	41.4633	1
GTATCAA	18275	0.0	33.112556	1
TCAACGC	20410	0.0	28.610348	4
ATCAACG	20740	0.0	28.19497	3
CAACGCA	21260	0.0	27.428392	5
AACGCAG	22125	0.0	26.356047	6
TATCAAC	22770	0.0	26.110857	2
GTACATG	29285	0.0	22.16534	1
ACGCAGA	26370	0.0	22.128551	7
CGCAGAG	27085	0.0	21.544395	8
TACATGG	29385	0.0	21.341568	2
ACATGGG	29760	0.0	20.566103	3
CATGGGG	21750	0.0	18.478848	4
GCAGAGT	33885	0.0	17.124756	9
GAGTACT	19245	0.0	16.017376	12-13
AGAGTAC	29585	0.0	16.000711	10-11
CAGAGTA	32655	0.0	15.537683	10-11
GAGTACA	20805	0.0	15.247584	1
GTGGTAT	6510	0.0	15.137902	1
ATGGGGG	13480	0.0	14.560548	5
>>END_MODULE