FastQCFastQC Report
Fri 27 May 2016
SRR2139768_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139768_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8740555
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78700.046.1176761
GTATCAA133100.033.75351
TCAACGC147300.029.268234
ATCAACG150950.028.5892723
CAACGCA153300.028.094395
AACGCAG161200.026.8783386
TATCAAC168400.026.1142922
GTACATG214350.022.7711661
ACGCAGA193450.022.4420727
TACATGG213150.022.0894832
CGCAGAG197800.021.8830768
ACATGGG212900.021.6081583
CATGGGG165150.018.6313654
GAGTACT137350.017.68946512-13
GCAGAGT249700.017.2482789
AGAGTAC210600.016.6290110-11
CAGAGTA238400.016.55440710-11
GAGTACA152850.015.9525151
GTACTTT155050.015.80971114-15
GTGGTAT45750.015.5646371
Produced by FastQC (version 0.11.5)