##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139768_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8740555
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.56706444842462	34.0	31.0	34.0	31.0	34.0
2	32.74590583778719	34.0	31.0	34.0	31.0	34.0
3	32.83704947797937	34.0	31.0	34.0	31.0	34.0
4	36.23809391966528	37.0	37.0	37.0	35.0	37.0
5	36.09511421185497	37.0	35.0	37.0	35.0	37.0
6	36.11934184957362	37.0	36.0	37.0	35.0	37.0
7	36.13914505429003	37.0	36.0	37.0	35.0	37.0
8	36.13459820343216	37.0	36.0	37.0	35.0	37.0
9	38.002069090578345	39.0	38.0	39.0	35.0	39.0
10-11	37.889392721629235	39.0	38.0	39.0	35.0	39.0
12-13	37.921914340679734	39.0	38.0	39.0	35.0	39.0
14-15	39.28617742237192	40.0	39.0	41.0	36.0	41.0
16-17	39.25906524242454	40.0	39.0	41.0	36.0	41.0
18-19	39.28278021246935	40.0	39.0	41.0	36.0	41.0
20-21	39.31929747194785	40.0	39.0	41.0	36.0	41.0
22-23	39.28510131175419	40.0	39.0	41.0	36.0	41.0
24-25	39.28078685283353	40.0	39.0	41.0	36.0	41.0
26-27	39.191677215712815	40.0	39.0	41.0	36.0	41.0
28-29	39.12108951026373	40.0	39.0	41.0	36.0	41.0
30-31	39.01363803171665	40.0	39.0	41.0	36.0	41.0
32-33	38.96401256621593	40.0	38.0	41.0	35.0	41.0
34-35	38.91173619276876	40.0	38.0	41.0	35.0	41.0
36-37	38.84944601289935	40.0	38.0	41.0	35.0	41.0
38-39	38.77290831819496	40.0	38.0	41.0	35.0	41.0
40-41	38.75195650890687	40.0	38.0	41.0	35.0	41.0
42-43	38.69652039285759	40.0	38.0	41.0	35.0	41.0
44-45	38.67252146660002	40.0	38.0	41.0	35.0	41.0
46-47	38.57220699189747	40.0	38.0	41.0	34.5	41.0
48-49	38.55950191521792	40.0	38.0	41.0	34.5	41.0
50-51	38.495630689752424	40.0	38.0	41.0	34.0	41.0
52-53	38.331151064158945	40.0	38.0	41.0	34.0	41.0
54-55	38.19744291808064	40.0	37.0	41.0	34.0	41.0
56-57	38.01506675469899	40.0	37.0	41.0	33.5	41.0
58-59	37.80412923357983	39.5	36.5	41.0	33.0	41.0
60-61	37.64912409450744	39.0	36.0	41.0	33.0	41.0
62-63	37.4816952482388	39.0	36.0	41.0	33.0	41.0
64-65	37.213647450071605	39.0	35.0	40.0	32.5	41.0
66-67	37.00840958766653	38.0	35.0	40.0	32.0	41.0
68-69	36.724409631058734	37.5	35.0	40.0	32.0	41.0
70-71	36.386867886746174	37.0	35.0	39.5	32.0	41.0
72-73	36.118584490321055	36.5	35.0	39.0	32.0	41.0
74-75	35.75084422563786	36.0	34.5	39.0	31.5	40.5
76-77	33.74583521508844	34.5	32.0	36.5	29.0	39.0
78-79	34.870015547464234	35.0	34.0	37.0	30.5	39.0
80-81	34.82753278021239	35.0	34.0	37.0	31.0	39.0
82-83	34.577432864303645	35.0	34.0	36.5	31.0	39.0
84-85	34.29540504461035	35.0	34.0	36.0	31.0	37.0
86-87	34.07519319137107	35.0	34.0	36.0	31.0	37.0
88-89	33.866511939304544	35.0	34.0	35.0	30.0	36.5
90-91	33.80868300456184	35.0	34.0	35.0	30.5	36.0
92-93	33.722479519081226	35.0	34.0	35.0	30.5	36.0
94-95	33.63817996349867	35.0	34.0	35.0	30.0	36.0
96-97	33.64031845062598	35.0	34.0	35.0	30.5	35.5
98-99	33.69768355515852	35.0	34.0	35.0	31.0	35.0
100-101	32.43648078046918	34.0	31.5	34.5	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	12.0
20	36.0
21	156.0
22	567.0
23	1913.0
24	5064.0
25	11434.0
26	21415.0
27	35947.0
28	53638.0
29	75439.0
30	101880.0
31	134884.0
32	178251.0
33	245729.0
34	363605.0
35	579481.0
36	1084838.0
37	2154152.0
38	2661938.0
39	1012144.0
40	18029.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.6277278731156	19.7433114945218	13.01167946429031	24.61728116807228
2	15.215083178248651	21.289403008059367	40.17550254645504	23.32001126723694
3	18.825000340374334	25.33798027247566	30.204074721262025	25.63294466588798
4	12.718063573614044	17.386187744357798	38.50860927728155	31.387139404746613
5	13.251265308812233	37.725320385870276	35.840291130287625	13.183123175029868
6	30.86185679485578	37.596647523649615	18.44250985410028	13.09898582739433
7	27.11860974503335	32.15660790418915	22.896681045997653	17.828101304779846
8	24.796935021952844	36.650820222796	21.136822937498685	17.41542181775247
9	25.618815803398537	16.39327157121569	21.01045417752366	36.97745844786212
10-11	24.377429027449715	26.61383755718131	29.036499531291625	19.972233884077355
12-13	24.93914710472018	24.02077765054824	29.161286329325787	21.878788915405796
14-15	21.964372389502838	25.634884064823133	26.405624816051386	25.99511872962264
16-17	21.920289969067625	28.7973190606484	27.1818027729133	22.100588197370673
18-19	21.995954490304108	28.11670998008708	28.532970732407726	21.354364797201093
20-21	22.58468931699704	27.38485375238653	28.473305735982805	21.55715119463363
22-23	22.267436624856156	27.348718994973197	28.32424360212643	22.05960077804422
24-25	22.121863235649418	27.404389624590287	28.41374393745922	22.06000320230108
26-27	22.1025191960953	27.59207608404636	28.34070848765859	21.964696232199753
28-29	22.090927884455382	27.631723583208885	28.333553962658293	21.943794569677443
30-31	22.25679856801734	27.53881765989586	28.30780056126672	21.89658321082008
32-33	22.035659137875378	27.683942512762833	28.282325304850293	21.9980730445115
34-35	22.245207618790477	27.67141491749035	28.228751860494217	21.854625603224957
36-37	22.189907493888725	27.651574437621466	28.169059004886705	21.989459063603103
38-39	22.02032884690516	27.701865545241063	28.32601345736343	21.951792150490355
40-41	22.255754501164326	27.579611997014595	28.200116944920815	21.964516556900264
42-43	22.14964609192594	27.635400966374874	28.0925722497744	22.122380691924786
44-45	22.164470925005908	27.74434864755364	27.99211819338584	22.099062234054614
46-47	22.274680336235775	27.683493099873907	27.74374298426428	22.298083579626034
48-49	22.176209415760265	27.755546837381946	27.810861174712137	22.257382572145655
50-51	22.156608120315198	27.87721107060862	27.775756538311036	22.190424270765146
52-53	22.323269169990915	27.79133927737228	27.65737737618596	22.228014176450845
54-55	22.274167861310897	27.684294392718645	27.714009999384853	22.327527746585602
56-57	22.248356093209193	27.82681681627982	27.665480987407065	22.259346103103926
58-59	22.254836353169928	27.817511454417755	27.706910819008467	22.22074137340385
60-61	22.3044746600118	27.783469606109712	27.691820133179323	22.220235600699166
62-63	22.258663522463237	27.894671049282564	27.643241118208927	22.203424310045268
64-65	22.226737686920224	27.861461187723496	27.58422446509517	22.327576660261112
66-67	22.251079527141883	27.911205666483045	27.5003325423595	22.33738226401557
68-69	22.236277853781765	27.90467539487705	27.5037650332422	22.35528171809898
70-71	22.24166667434211	27.727589892469695	27.571366146591902	22.459377286596297
72-73	22.3020834453426	27.728774692318016	27.507046129497446	22.462095732841938
74-75	22.190988121184237	27.866838189375294	27.46318891497112	22.478984774469353
76-77	22.321629813962716	27.79893714600135	27.42025081711225	22.459182222923683
78-79	22.30970940680102	27.779675769388973	27.37263498880026	22.53797983500975
80-81	22.303870508459493	27.796638790535923	27.395944519169973	22.50354618183461
82-83	22.348012332365556	27.79625647574764	27.455340325161544	22.40039086672526
84-85	22.43296728357864	27.79343471100997	27.391855626565036	22.381742378846354
86-87	22.307168944722378	27.784921863699356	27.503354660547245	22.404554531031017
88-89	22.3655373840159	27.784831109931034	27.453416193640518	22.39621531241254
90-91	22.379526120584245	27.835783181869665	27.462061474207612	22.322629223338474
92-93	22.410208439965405	27.78710502009095	27.516369861804833	22.286316678138807
94-95	22.31585155624188	27.847106918679685	27.533571562855336	22.303469962223094
96-97	22.31402566104084	27.84059991966715	27.572985426408287	22.27238899288372
98-99	22.561325142950437	28.401294650216	27.648154967577852	21.389225239255712
100-101	23.32282641712318	31.036443190073843	24.68202687215687	20.958703520646104
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1693.0
1	1415.5
2	1606.0
3	2218.5
4	2941.0
5	4686.0
6	6279.5
7	7432.0
8	8220.5
9	8166.5
10	7488.0
11	6857.5
12	7415.5
13	9840.0
14	15376.5
15	22406.0
16	27961.0
17	31495.0
18	32878.0
19	32192.0
20	30517.5
21	29356.0
22	29952.0
23	33792.5
24	41370.5
25	52332.0
26	67125.5
27	84021.5
28	102504.0
29	122553.0
30	142525.0
31	162486.5
32	182545.5
33	201838.0
34	220421.5
35	239169.0
36	260388.0
37	279017.5
38	293584.5
39	308358.5
40	323687.0
41	338475.5
42	348205.0
43	355909.5
44	364253.5
45	369232.5
46	371722.5
47	374081.0
48	372117.5
49	365804.0
50	358716.5
51	347177.5
52	331020.5
53	313805.0
54	294782.0
55	273584.5
56	251124.0
57	227881.5
58	203488.5
59	176463.0
60	148030.5
61	120794.5
62	95721.5
63	73936.5
64	55831.0
65	41666.5
66	31210.0
67	23896.5
68	18298.5
69	13539.5
70	9843.0
71	7139.5
72	5146.5
73	3574.5
74	2407.5
75	1618.0
76	1113.5
77	766.5
78	533.0
79	368.5
80	251.0
81	179.0
82	129.0
83	83.5
84	54.0
85	38.5
86	32.0
87	27.5
88	17.5
89	10.5
90	9.0
91	8.5
92	8.5
93	5.5
94	1.5
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0019335156634790354
3	0.002036483953250108
4	4.805186855983401E-4
5	1.1440921085674765E-5
6	3.546685536559177E-4
7	0.0
8	3.4322763257024296E-5
9	3.4322763257024297E-4
10-11	5.205619093982018E-4
12-13	1.0868875031391027E-4
14-15	2.8602302714186916E-5
16-17	4.175936196271289E-4
18-19	0.0
20-21	9.153088726952952E-5
22-23	2.2884994549366422E-5
24-25	0.0
26-27	8.586736514616172E-5
28-29	2.176693059070008E-4
30-31	6.137096424969265E-4
32-33	3.333821910682313E-4
34-35	0.0010375600783311745
36-37	1.2155391046165307E-4
38-39	1.5718065867544674E-4
40-41	3.1622462465747455E-4
42-43	0.0013199414275991504
44-45	3.442550830450098E-4
46-47	2.094915096383153E-4
48-49	3.198396794554663E-4
50-51	0.0
52-53	4.347008061495837E-4
54-55	6.41029513122059E-4
56-57	7.450798651107412E-4
58-59	7.133051878123174E-4
60-61	8.072555625426607E-4
62-63	6.990691576407306E-5
64-65	8.45441561638166E-4
66-67	0.0026292604516979013
68-69	0.0014276348516827544
70-71	2.8947296052812496E-4
72-73	0.0
74-75	1.2721571272067406E-4
76-77	6.756343615474865E-6
78-79	0.0010978681242173002
80-81	1.9282982939725183E-4
82-83	1.6696543043374907E-4
84-85	8.852394541107674E-4
86-87	3.1938906691477754E-4
88-89	2.8704486166734025E-5
90-91	7.260961346925756E-6
92-93	1.9120197355735537E-4
94-95	6.857909920756851E-4
96-97	1.5157139368835495E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	230.0
20-21	706.0
22-23	1456.0
24-25	2838.0
26-27	4577.0
28-29	9159.0
30-31	17753.0
32-33	22564.0
34-35	32087.0
36-37	46873.0
38-39	51280.0
40-41	52068.0
42-43	58261.0
44-45	69420.0
46-47	69364.0
48-49	64753.0
50-51	57722.0
52-53	53213.0
54-55	58102.0
56-57	61277.0
58-59	63173.0
60-61	61412.0
62-63	60038.0
64-65	64923.0
66-67	69770.0
68-69	70279.0
70-71	68199.0
72-73	64949.0
74-75	67491.0
76-77	66548.0
78-79	71497.0
80-81	73623.0
82-83	70685.0
84-85	71013.0
86-87	75666.0
88-89	80571.0
90-91	86013.0
92-93	89472.0
94-95	101732.0
96-97	167120.0
98-99	770220.0
100-101	5692458.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.95545574402428
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.8139880092453	78.96771715276282
2	6.636387886105499	11.541402662608512
3	1.4659837796321735	3.8242586301378876
4	0.5209673847350758	1.812038254696438
5	0.22961587057678687	0.9983176336032697
6	0.11003616533882019	0.574094694321715
7	0.06416859648736561	0.39058666864092906
8	0.04296820091420461	0.29890555943963704
9	0.026272009187676682	0.2056045079003057
>10	0.08732869686997917	1.1829013021719408
>50	0.0018484510968618157	0.10635517889596392
>100	3.950549074062186E-4	0.06345652677753627
>500	1.9947462223800224E-5	0.0135294172151366
>1k	1.9947440581212122E-5	0.020831810827860398
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.1440921085674765E-5	0.0	0.0	0.0
3	0.0	1.1440921085674765E-5	0.0	0.0	0.0
4	0.0	2.288184217134953E-5	0.0	1.1440921085674765E-5	0.0
5	0.0	2.288184217134953E-5	0.0	3.4322763257024296E-5	0.0
6	4.576368434269906E-5	2.288184217134953E-5	0.0	3.4322763257024296E-5	0.0
7	4.576368434269906E-5	2.288184217134953E-5	0.0	5.7204605428373826E-5	0.0
8	4.576368434269906E-5	2.288184217134953E-5	0.0	5.7204605428373826E-5	0.0
9	4.576368434269906E-5	5.7204605428373826E-5	0.0	6.864552651404859E-5	1.1440921085674765E-5
10-11	6.292506597121121E-5	5.7204605428373826E-5	0.0	6.864552651404859E-5	1.1440921085674765E-5
12-13	6.864552651404859E-5	5.7204605428373826E-5	0.0	6.864552651404859E-5	1.7161381628512148E-5
14-15	6.864552651404859E-5	6.864552651404859E-5	0.0	6.864552651404859E-5	3.4322763257024296E-5
16-17	8.008644759972336E-5	8.008644759972336E-5	0.0	9.152736868539812E-5	3.4322763257024296E-5
18-19	8.008644759972336E-5	1.029682897710729E-4	0.0	1.3729105302809718E-4	4.004322379986168E-5
20-21	9.152736868539812E-5	1.3157059248525982E-4	0.0	2.288184217134953E-4	4.576368434269906E-5
22-23	1.1440921085674765E-4	1.6017289519944673E-4	0.0	3.3178671148456824E-4	4.576368434269906E-5
24-25	1.4873197411377194E-4	1.7161381628512149E-4	0.0	3.947117774557794E-4	4.576368434269906E-5
26-27	1.4873197411377194E-4	1.7161381628512149E-4	0.0	5.320028304838766E-4	4.576368434269906E-5
28-29	2.059365795421458E-4	1.7161381628512149E-4	0.0	0.0013729105302809719	6.292506597121121E-5
30-31	2.059365795421458E-4	2.4025934279917006E-4	0.0	0.0039928814589004925	8.008644759972336E-5
32-33	2.059365795421458E-4	2.68861645513357E-4	0.0	0.008866713841397943	8.008644759972336E-5
34-35	2.059365795421458E-4	3.260662509417308E-4	0.0	0.01584567570365955	8.008644759972336E-5
36-37	2.1737750062782055E-4	3.88991316912942E-4	0.0	0.025404565270740816	8.008644759972336E-5
38-39	2.1737750062782055E-4	3.88991316912942E-4	0.0	0.04176508242325573	8.008644759972336E-5
40-41	2.1737750062782055E-4	4.233140801699663E-4	0.0	0.07518401291451172	8.580690814256074E-5
42-43	2.3453888225633267E-4	4.233140801699663E-4	0.0	0.08902752742817818	9.152736868539812E-5
44-45	2.4025934279917006E-4	4.805186855983401E-4	0.0	0.10779635846922764	9.152736868539812E-5
46-47	2.4025934279917006E-4	5.37723291026714E-4	0.0	0.12784657267187266	9.152736868539812E-5
48-49	2.4597980334200746E-4	5.434437515695514E-4	0.0	0.14616348733003798	9.152736868539812E-5
50-51	2.574207244276822E-4	6.120892780835999E-4	0.0	0.1634621600115782	1.029682897710729E-4
52-53	2.631411849705196E-4	6.178097386264373E-4	0.0	0.1867158321182122	1.029682897710729E-4
54-55	2.7458210605619437E-4	6.235301991692748E-4	0.0	0.23248523692145406	1.0868875031391027E-4
56-57	2.974639482275439E-4	6.635734229691364E-4	0.0	0.28346598127922085	1.2012967139958503E-4
58-59	3.0890486931321867E-4	6.635734229691364E-4	0.0	0.333131019712135	1.4301151357093455E-4
60-61	3.260662509417308E-4	6.750143440548112E-4	0.0	0.36159031091275096	1.5445243465660933E-4
62-63	3.317867114845682E-4	6.864552651404859E-4	0.0	0.3859079886803527	1.6017289519944673E-4
64-65	3.317867114845682E-4	7.207780283975102E-4	0.0	0.396696777264144	1.6017289519944673E-4
66-67	3.4322763257024297E-4	7.207780283975102E-4	0.0	0.4022742262934104	1.6589335574228412E-4
68-69	3.4322763257024297E-4	7.379394100260224E-4	0.0	0.40791460038864813	1.7733427682795885E-4
70-71	3.4894809311308036E-4	7.436598705688598E-4	0.0	0.41336047882542926	2.0593657954214576E-4
72-73	3.775503958272673E-4	7.436598705688598E-4	0.0	0.41775379252232836	2.4025934279917006E-4
74-75	3.775503958272673E-4	7.722621732830467E-4	0.0	0.420968691347403	2.4025934279917006E-4
76-77	3.775503958272673E-4	8.065849365400709E-4	0.0	0.4237202328685078	2.4025934279917006E-4
78-79	3.775503958272673E-4	8.123053970829084E-4	0.0	0.42608850353324246	2.4025934279917006E-4
80-81	3.775503958272673E-4	8.123053970829084E-4	0.0	0.42714678873366735	2.5170026388484485E-4
82-83	3.775503958272673E-4	8.180258576257458E-4	0.0	0.4274728549846091	2.631411849705196E-4
84-85	3.775503958272673E-4	8.294667787114205E-4	0.0	0.4278504053804364	2.631411849705196E-4
86-87	3.88991316912942E-4	8.351872392542578E-4	0.0	0.4280449010388928	2.631411849705196E-4
88-89	3.88991316912942E-4	8.695100025112822E-4	0.0	0.4281192670259497	2.631411849705196E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7870	0.0	46.117676	1
GTATCAA	13310	0.0	33.7535	1
TCAACGC	14730	0.0	29.26823	4
ATCAACG	15095	0.0	28.589272	3
CAACGCA	15330	0.0	28.09439	5
AACGCAG	16120	0.0	26.878338	6
TATCAAC	16840	0.0	26.114292	2
GTACATG	21435	0.0	22.771166	1
ACGCAGA	19345	0.0	22.442072	7
TACATGG	21315	0.0	22.089483	2
CGCAGAG	19780	0.0	21.883076	8
ACATGGG	21290	0.0	21.608158	3
CATGGGG	16515	0.0	18.631365	4
GAGTACT	13735	0.0	17.689465	12-13
GCAGAGT	24970	0.0	17.248278	9
AGAGTAC	21060	0.0	16.62901	10-11
CAGAGTA	23840	0.0	16.554407	10-11
GAGTACA	15285	0.0	15.952515	1
GTACTTT	15505	0.0	15.809711	14-15
GTGGTAT	4575	0.0	15.564637	1
>>END_MODULE