##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139767_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8896047
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.53451167692796	34.0	31.0	34.0	31.0	34.0
2	32.725290907298486	34.0	31.0	34.0	31.0	34.0
3	32.815213206494974	34.0	31.0	34.0	31.0	34.0
4	36.22666786719989	37.0	37.0	37.0	35.0	37.0
5	36.07563527935498	37.0	35.0	37.0	35.0	37.0
6	36.10152666684427	37.0	36.0	37.0	35.0	37.0
7	36.1172855763914	37.0	36.0	37.0	35.0	37.0
8	36.113635415820085	37.0	36.0	37.0	35.0	37.0
9	37.98016006435218	39.0	38.0	39.0	35.0	39.0
10-11	37.86919735248701	39.0	38.0	39.0	35.0	39.0
12-13	37.89655017560047	39.0	38.0	39.0	35.0	39.0
14-15	39.23169391978257	40.0	39.0	41.0	36.0	41.0
16-17	39.19870185038366	40.0	39.0	41.0	36.0	41.0
18-19	39.22925823121213	40.0	39.0	41.0	36.0	41.0
20-21	39.26902223185548	40.0	39.0	41.0	36.0	41.0
22-23	39.22528373864748	40.0	39.0	41.0	36.0	41.0
24-25	39.20961406387757	40.0	39.0	41.0	36.0	41.0
26-27	39.1009709227964	40.0	39.0	41.0	36.0	41.0
28-29	39.02120288727028	40.0	39.0	41.0	35.5	41.0
30-31	38.90400233949535	40.0	38.0	41.0	35.0	41.0
32-33	38.83629195443134	40.0	38.0	41.0	35.0	41.0
34-35	38.775712595637046	40.0	38.0	41.0	35.0	41.0
36-37	38.698113233499356	40.0	38.0	41.0	35.0	41.0
38-39	38.59759561352862	40.0	38.0	41.0	35.0	41.0
40-41	38.60133558293211	40.0	38.0	41.0	35.0	41.0
42-43	38.54911308614517	40.0	38.0	41.0	34.5	41.0
44-45	38.51264433989493	40.0	38.0	41.0	34.0	41.0
46-47	38.405414484555884	40.0	38.0	41.0	34.0	41.0
48-49	38.3867503685273	40.0	38.0	41.0	34.0	41.0
50-51	38.28160316705531	40.0	38.0	41.0	34.0	41.0
52-53	38.10119850300492	40.0	37.0	41.0	33.5	41.0
54-55	37.94653167449431	40.0	37.0	41.0	33.0	41.0
56-57	37.724057798549104	39.0	36.0	41.0	33.0	41.0
58-59	37.48376309596286	39.0	36.0	41.0	33.0	41.0
60-61	37.30178865253926	39.0	35.0	40.5	32.5	41.0
62-63	37.11951868734418	39.0	35.0	40.0	32.0	41.0
64-65	36.84936553748852	38.0	35.0	40.0	32.0	41.0
66-67	36.63749485111709	37.5	35.0	40.0	32.0	41.0
68-69	36.35420339684911	37.0	35.0	39.5	32.0	41.0
70-71	36.01529430763911	36.5	35.0	39.0	31.0	41.0
72-73	35.752178769627804	36.0	35.0	39.0	31.0	40.5
74-75	35.40093173867737	35.5	34.0	38.5	31.0	40.0
76-77	33.4390990108269	34.5	32.0	36.0	28.0	39.0
78-79	34.57004885259239	35.0	34.0	37.0	30.5	39.0
80-81	34.558719653967806	35.0	34.0	36.5	31.0	39.0
82-83	34.340829920869425	35.0	34.0	36.0	31.0	37.5
84-85	34.09248978684852	35.0	34.0	36.0	30.5	37.0
86-87	33.90281500053902	35.0	34.0	35.0	30.0	37.0
88-89	33.71982049004977	35.0	34.0	35.0	30.0	36.0
90-91	33.686369345832205	35.0	34.0	35.0	30.0	36.0
92-93	33.623499461315404	35.0	34.0	35.0	30.0	36.0
94-95	33.54951563679894	35.0	34.0	35.0	30.0	36.0
96-97	33.56233276559584	35.0	34.0	35.0	30.0	35.0
98-99	33.62954566700071	35.0	34.0	35.0	31.0	35.0
100-101	32.35442202677988	34.0	31.5	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	5.0
19	23.0
20	47.0
21	163.0
22	719.0
23	2242.0
24	5979.0
25	12861.0
26	23764.0
27	39328.0
28	58997.0
29	82961.0
30	109969.0
31	145243.0
32	194174.0
33	268565.0
34	405135.0
35	648924.0
36	1212092.0
37	2279290.0
38	2472332.0
39	916476.0
40	16758.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.57713566486328	19.56654455625066	12.952561963757612	24.90375781512845
2	15.419965437867017	20.815001310159566	39.85426709021935	23.910766161754065
3	18.608404944561137	25.590784901023905	30.22062571978909	25.58018443462587
4	12.901399324910376	17.547957487429187	36.82438766889044	32.72625551877
5	13.399416639575973	37.6378646508493	35.296340710545046	13.666377999029681
6	31.136416085816442	36.64922401737138	18.090018099123302	14.124341797688874
7	27.29483106372977	32.004642061805654	22.037597148486288	18.66292972597829
8	25.651025478521795	35.58430816760982	20.898979568666604	17.865686785201778
9	25.887549187759824	15.937407472605148	20.160321240501645	38.01472209913339
10-11	24.92590623540951	25.972838440774137	28.25700204412291	20.844253279693444
12-13	25.66936843211611	23.393405039436107	28.27489999758319	22.662326530864593
14-15	22.681212159090723	24.827891185559064	25.423880852201318	27.06701580314889
16-17	22.718799318098903	28.20687675594844	26.099651512365963	22.974672413586696
18-19	22.830584190933344	27.195118236223347	27.630092332021178	22.34420524082213
20-21	23.314708372160705	26.408609528772875	27.510920049549902	22.765762049516518
22-23	23.07563841747823	26.82572364885986	27.39429074252568	22.70434719113623
24-25	23.024724580235628	27.046834854296964	27.483900111193478	22.44454045427393
26-27	22.97459130812002	27.273275457067104	27.472226997244185	22.279906237568692
28-29	22.782773900978274	27.066078004955465	27.482772201357154	22.668375892709108
30-31	23.28710837069618	26.789080893412194	27.488286129619766	22.435524606271866
32-33	22.815690987627033	27.024945600214036	27.615412798907773	22.543950613251162
34-35	22.91040811715914	26.631156439315923	27.80473370902021	22.65370173450473
36-37	22.709548063737138	27.094916535417994	27.580961593260955	22.614573807583913
38-39	22.656783948367277	26.57015131786596	27.866367493523136	22.906697240243627
40-41	22.790836383854884	26.410570370595472	27.985063546482188	22.81352969906746
42-43	22.973016630203176	26.416955071180205	27.806122315335006	22.80390598328161
44-45	22.618944647218555	26.760187233739703	27.711118443432365	22.90974967560938
46-47	23.196377561585134	26.367565227670852	27.1637801712436	23.27227703950041
48-49	22.89740719714404	26.5507080277846	27.170003628823192	23.381881146248162
50-51	22.72925567990122	26.74831747197388	27.44083572825428	23.081591119870616
52-53	22.903132502171154	26.700949020081815	27.58636969724734	22.809548780499693
54-55	22.744679188106918	26.783115966859366	27.200465245442206	23.27173959959151
56-57	23.12896854949694	27.09103106884325	26.764296815346295	23.015703566313515
58-59	22.641205811493247	27.357210651483406	27.309078636821226	22.692504900202117
60-61	23.089462827596787	27.60247075103277	26.63474980342041	22.673316617950032
62-63	22.798793311155855	27.88121612670553	26.622561418931877	22.69742914320674
64-65	22.79843115113401	27.62670682695787	26.676911533102654	22.897950488805467
66-67	22.927726235038392	27.359246911025124	26.667589428199523	23.045437425736957
68-69	22.94684217865751	27.160217370699357	26.806024208339597	23.086916242303538
70-71	22.9406825245963	26.990067417185813	26.834038106814806	23.23521195140308
72-73	23.011824431965593	26.972997942995786	26.743925404127594	23.27125222091103
74-75	22.88441162284445	27.078521208046414	26.797750849557318	23.23931631955182
76-77	23.1505234771365	26.91504483125374	26.73018559772917	23.204246093880585
78-79	23.070506294013736	26.899939717414366	26.668332093701874	23.361221894870024
80-81	23.11732357165816	26.99969129616317	26.615924651386578	23.26706048079209
82-83	23.09450731504306	26.9602654704357	26.731627499897098	23.21359971462414
84-85	23.15176480123133	26.937747663552752	26.71127217062382	23.1992153645921
86-87	23.092333238264022	26.879142316567663	26.821370363864705	23.207154081303617
88-89	23.096711118942086	26.90269022541676	26.775058320884742	23.22554033475641
90-91	23.159234267025926	26.919892371901565	26.784395347992902	23.136478013079607
92-93	23.18755631258905	26.803008600882176	26.846282477230165	23.163152609298606
94-95	23.131830286844497	26.800131745056362	26.891619655043108	23.17641831305603
96-97	23.149865798493085	26.76112131769178	26.89756539726077	23.191447486554363
98-99	23.38377614786745	27.373404251502258	26.968618481930644	22.27420111869965
100-101	24.213499216141642	29.930241780204785	23.91634631091348	21.93991269274009
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1668.0
1	1526.5
2	2163.0
3	3086.0
4	4083.5
5	6669.5
6	9007.5
7	10826.5
8	12347.5
9	12418.5
10	11008.5
11	9452.5
12	9909.5
13	13101.5
14	20714.5
15	30695.0
16	38387.0
17	42597.5
18	43173.5
19	40495.0
20	36482.5
21	32600.0
22	30493.0
23	32201.0
24	38051.0
25	47341.0
26	58986.0
27	71827.0
28	86721.5
29	104620.0
30	121392.5
31	137891.0
32	154667.5
33	171832.5
34	188402.0
35	202979.0
36	220472.0
37	236992.5
38	252672.0
39	268163.5
40	281364.5
41	295924.5
42	308403.5
43	320920.0
44	336203.0
45	355396.0
46	382248.5
47	407272.0
48	414243.0
49	413370.0
50	413722.5
51	405387.5
52	389374.0
53	370651.0
54	354869.0
55	336897.5
56	313769.5
57	287900.0
58	259561.5
59	228704.0
60	195437.5
61	163452.5
62	132869.0
63	103693.0
64	78810.0
65	59801.5
66	45459.5
67	35068.5
68	26808.0
69	19442.0
70	13943.0
71	10081.0
72	7188.0
73	5117.5
74	3691.5
75	2645.5
76	1856.5
77	1223.0
78	793.5
79	540.5
80	368.0
81	257.5
82	177.5
83	125.5
84	97.0
85	70.5
86	53.5
87	40.0
88	30.0
89	20.0
90	13.5
91	9.5
92	4.5
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0020683343961649483
3	0.002383080934711788
4	4.8336075562550423E-4
5	3.372284341573285E-5
6	4.0467412098879423E-4
7	0.0
8	5.6204739026221424E-5
9	3.709512775730614E-4
10-11	6.238726031910578E-4
12-13	1.348913736629314E-4
14-15	3.9343317318354995E-5
16-17	3.990536470861721E-4
18-19	0.0
20-21	4.496563360933802E-5
22-23	1.124239037727832E-5
24-25	0.0
26-27	6.750026493853988E-5
28-29	2.4199844794576804E-4
30-31	7.214466764388873E-4
32-33	3.5604231500747745E-4
34-35	9.413253747864297E-4
36-37	1.2535583250204374E-4
38-39	1.2046764161702683E-4
40-41	2.542014590470473E-4
42-43	0.0013388038020863803
44-45	3.347097537945516E-4
46-47	1.2456135127000975E-4
48-49	3.6533653424035813E-4
50-51	0.0
52-53	4.995330584277014E-4
54-55	6.201105663279471E-4
56-57	7.372919218145906E-4
58-59	7.823738311100251E-4
60-61	8.354246374826682E-4
62-63	8.964039411295676E-5
64-65	7.064034369832399E-4
66-67	0.0027117422576311413
68-69	0.0012961036531977568
70-71	3.02342407883155E-4
72-73	0.0
74-75	8.9078984965523E-5
76-77	6.921233319481639E-6
78-79	9.788308257318962E-4
80-81	1.6961720506465666E-4
82-83	1.0718733222949437E-4
84-85	8.090150862532879E-4
86-87	2.4832383239046965E-4
88-89	2.9563232798633E-5
90-91	2.994243043755847E-5
92-93	1.3663018944231201E-4
94-95	7.089646265182053E-4
96-97	1.5682323794193965E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	256.0
20-21	617.0
22-23	1409.0
24-25	3877.0
26-27	4198.0
28-29	9423.0
30-31	22659.0
32-33	27826.0
34-35	37600.0
36-37	55676.0
38-39	62164.0
40-41	63873.0
42-43	71252.0
44-45	84228.0
46-47	82875.0
48-49	76763.0
50-51	68741.0
52-53	61656.0
54-55	71063.0
56-57	79669.0
58-59	86413.0
60-61	93956.0
62-63	92366.0
64-65	94289.0
66-67	99001.0
68-69	83021.0
70-71	75757.0
72-73	70490.0
74-75	73327.0
76-77	71434.0
78-79	75950.0
80-81	78042.0
82-83	75393.0
84-85	75471.0
86-87	79353.0
88-89	84493.0
90-91	91345.0
92-93	95304.0
94-95	106331.0
96-97	164528.0
98-99	756573.0
100-101	5487385.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.38582044257882
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.93980033367426	72.4499260004632
2	8.015630588085365	13.207486047280865
3	2.1976504434954522	5.431657045001101
4	0.8631914159737726	2.8445893201596237
5	0.40354252449982686	1.662309098219385
6	0.20731547635314518	1.024791336587875
7	0.12109796416324152	0.6983728592060261
8	0.0712300649212932	0.46946778709751147
9	0.04455422079116773	0.33035724306541403
>10	0.13282311212655695	1.6546702684120733
>50	0.0025510213540734165	0.1370060591893135
>100	5.888478810099912E-4	0.07695663555741608
>500	2.398668085825965E-5	0.012410299760228696
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.1240947805244285E-5	0.0	1.1240947805244285E-5	0.0
3	0.0	1.1240947805244285E-5	0.0	2.248189561048857E-5	0.0
4	0.0	6.74456868314657E-5	0.0	4.496379122097714E-5	2.248189561048857E-5
5	0.0	1.2365042585768714E-4	0.0	4.496379122097714E-5	2.248189561048857E-5
6	1.5737326927341998E-4	1.2365042585768714E-4	0.0	4.496379122097714E-5	2.248189561048857E-5
7	1.5737326927341998E-4	1.348913736629314E-4	0.0	4.496379122097714E-5	2.248189561048857E-5
8	1.6861421707866427E-4	1.348913736629314E-4	0.0	4.496379122097714E-5	3.372284341573285E-5
9	1.6861421707866427E-4	2.3605990391012998E-4	0.0	1.0116853024719856E-4	3.372284341573285E-5
10-11	1.7985516488390853E-4	2.3605990391012998E-4	0.0	1.9671658659177498E-4	3.372284341573285E-5
12-13	2.0233706049439711E-4	2.6978274732586285E-4	0.0	2.3605990391012998E-4	3.372284341573285E-5
14-15	2.248189561048857E-4	4.496379122097714E-4	0.0	3.0912606464421783E-4	5.058426512359928E-5
16-17	3.035055907415957E-4	4.7211980782025997E-4	0.0	4.946017034307485E-4	5.6204739026221424E-5
18-19	3.035055907415957E-4	5.002221773333707E-4	0.0	7.868663463670999E-4	8.992758244195427E-5
20-21	3.0912606464421783E-4	5.170835990412371E-4	0.0	0.0016018350622473104	1.0116853024719856E-4
22-23	3.1474653854683996E-4	5.620473902622142E-4	0.0	0.002843959794726804	1.236504258576871E-4
24-25	3.1474653854683996E-4	6.182521292884357E-4	0.0	0.004929155612599618	1.348913736629314E-4
26-27	3.1474653854683996E-4	6.632159205094128E-4	0.0	0.009408673312989467	1.461323214681757E-4
28-29	3.1474653854683996E-4	6.632159205094128E-4	0.0	0.037482940456587066	1.461323214681757E-4
30-31	3.1474653854683996E-4	7.362820812435006E-4	0.0	0.05283245468464813	1.461323214681757E-4
32-33	3.1474653854683996E-4	7.53143502951367E-4	0.0	0.058132561574820815	1.5737326927341998E-4
34-35	3.3722843415732854E-4	8.824144027116763E-4	0.0	0.06457924514112842	1.6861421707866427E-4
36-37	3.3722843415732854E-4	0.0010397876719850964	0.0	0.07428018309705423	1.6861421707866427E-4
38-39	3.3722843415732854E-4	0.00110161288491394	0.0	0.09174299551250123	1.6861421707866427E-4
40-41	3.3722843415732854E-4	0.001225263310771627	0.0	0.13534101157514117	1.6861421707866427E-4
42-43	3.484693819625728E-4	0.0012814680497978486	0.0	0.15160104257542706	1.6861421707866427E-4
44-45	3.484693819625728E-4	0.0014051184756555357	0.0	0.17345906558272456	1.742346909812864E-4
46-47	3.5408985586519494E-4	0.0015175279537079783	0.0	0.19700323076080872	1.854756387865307E-4
48-49	3.597103297678171E-4	0.0015512507971237113	0.0	0.2167254736851098	2.248189561048857E-4
50-51	3.990536470861721E-4	0.001663660275176154	0.0	0.23600369917110375	2.248189561048857E-4
52-53	4.0467412098879423E-4	0.001703003592494509	0.0	0.2663317763496528	2.529213256179964E-4
54-55	4.1591506879403855E-4	0.001747967383715486	0.0	0.33109087665566517	2.810236951311071E-4
56-57	4.271560165992828E-4	0.0017648288054233525	0.0	0.3997168630066815	2.8664416903372925E-4
58-59	4.271560165992828E-4	0.0017648288054233525	0.0	0.4623626651253079	2.978851168389735E-4
60-61	4.664993339176378E-4	0.0018097925966443298	0.0	0.4972489466388835	3.035055907415957E-4
62-63	5.5080644245697E-4	0.0018435154400600626	0.0	0.5157009624611921	3.1474653854683996E-4
64-65	5.564269163595921E-4	0.0019952682354308603	0.0	0.5273353434396199	3.1474653854683996E-4
66-67	5.845292858727028E-4	0.0020346115527492153	0.0	0.5329895401856577	3.203670124494621E-4
68-69	6.013907075805693E-4	0.0020627139222623263	0.0	0.5381884785455833	3.259874863520842E-4
70-71	6.407340248989243E-4	0.0020964367656780593	0.0	0.5430501884713514	3.484693819625728E-4
72-73	7.868663463670999E-4	0.00212453913519117	0.0	0.5472543029505127	4.496379122097714E-4
74-75	7.868663463670999E-4	0.0021414005568990363	0.0	0.5509019905133146	4.552583861123935E-4
76-77	7.981072941723442E-4	0.002208846243730502	0.0	0.553734709360236	4.6087886001501565E-4
78-79	8.093482419775885E-4	0.002259430508854101	0.0	0.5566910786330153	4.6087886001501565E-4
80-81	8.093482419775885E-4	0.0022762919305619675	0.0	0.5579275828915922	4.721198078202599E-4
82-83	8.149687158802106E-4	0.002293153352269834	0.0	0.5584559074384388	5.170835990412371E-4
84-85	8.205891897828327E-4	0.0023156352478803225	0.0	0.5588605815594274	5.170835990412371E-4
86-87	8.205891897828327E-4	0.0023493580912960555	0.0	0.5590797800416297	5.170835990412371E-4
88-89	8.655529810038099E-4	0.0024842494649589867	0.0	0.559135984780656	5.283245468464813E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10605	0.0	44.366264	1
CTTATAC	14535	0.0	36.978115	1
GTATCAA	18455	0.0	35.5777	1
TATACAC	17220	0.0	32.565845	3
TTATACA	17645	0.0	31.037119	2
TCAACGC	21590	0.0	29.605982	4
ATCAACG	21970	0.0	29.036356	3
CAACGCA	22430	0.0	28.440392	5
AACGCAG	23115	0.0	27.707602	6
TATCAAC	23515	0.0	27.417305	2
TACACAT	22480	0.0	24.98319	5
GTACATG	28940	0.0	24.328293	1
TACATGG	28680	0.0	23.839426	2
ACACATC	23195	0.0	23.372465	6
ACGCAGA	27545	0.0	23.266705	7
ACATGGG	29345	0.0	22.952261	3
CGCAGAG	27960	0.0	22.921366	8
ACATCTC	24340	0.0	22.359924	8
ATACACA	26145	0.0	22.32421	4
CACATCT	25670	0.0	20.986769	7
>>END_MODULE