FastQCFastQC Report
Fri 27 May 2016
SRR2139766_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139766_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9551717
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA137700.030.7599161
GTATCAA199550.026.5539341
TCAACGC195400.025.9321864
ATCAACG197550.025.6943023
CAACGCA200750.025.3690855
AACGCAG207900.024.6409456
GTACATG241200.023.9060121
TACATGG237600.023.636712
CTTATAC143100.023.4875951
ACATGGG233700.023.1322843
TATCAAC240450.021.5736052
ACGCAGA243700.020.9329687
CGCAGAG246300.020.72948
TATACAC187950.020.3234523
CATGGGG169900.020.009034
TTATACA189550.018.7722552
GAGTACT160950.017.45870412-13
AGAGTAC241450.016.91104110-11
TACACAT226400.016.8522365
GCAGAGT305900.016.7886879
Produced by FastQC (version 0.11.5)