##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139766_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9551717
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.52619000332611	34.0	31.0	34.0	31.0	34.0
2	32.71574419552003	34.0	31.0	34.0	31.0	34.0
3	32.806976379220615	34.0	31.0	34.0	31.0	34.0
4	36.21770002189135	37.0	37.0	37.0	35.0	37.0
5	36.0693189507185	37.0	35.0	37.0	35.0	37.0
6	36.09256733632288	37.0	36.0	37.0	35.0	37.0
7	36.10844374890923	37.0	35.0	37.0	35.0	37.0
8	36.10518810387703	37.0	36.0	37.0	35.0	37.0
9	37.971875004253164	39.0	38.0	39.0	35.0	39.0
10-11	37.856615883824865	39.0	38.0	39.0	35.0	39.0
12-13	37.88838022525165	39.0	38.0	39.0	35.0	39.0
14-15	39.23227766274901	40.0	39.0	41.0	36.0	41.0
16-17	39.20027336446421	40.0	39.0	41.0	36.0	41.0
18-19	39.22961636112125	40.0	39.0	41.0	36.0	41.0
20-21	39.26643250400649	40.0	39.0	41.0	36.0	41.0
22-23	39.23058102748857	40.0	39.0	41.0	36.0	41.0
24-25	39.22580894014341	40.0	39.0	41.0	36.0	41.0
26-27	39.13026764247739	40.0	39.0	41.0	36.0	41.0
28-29	39.03908412042226	40.0	39.0	41.0	36.0	41.0
30-31	38.936421831961084	40.0	38.5	41.0	35.0	41.0
32-33	38.8819707070501	40.0	38.0	41.0	35.0	41.0
34-35	38.8220638670099	40.0	38.0	41.0	35.0	41.0
36-37	38.7613155636045	40.0	38.0	41.0	35.0	41.0
38-39	38.66837776771029	40.0	38.0	41.0	35.0	41.0
40-41	38.66241511489292	40.0	38.0	41.0	35.0	41.0
42-43	38.610443641557666	40.0	38.0	41.0	35.0	41.0
44-45	38.579668047149106	40.0	38.0	41.0	35.0	41.0
46-47	38.48101109553403	40.0	38.0	41.0	34.0	41.0
48-49	38.45950882897432	40.0	38.0	41.0	34.0	41.0
50-51	38.38235435826904	40.0	38.0	41.0	34.0	41.0
52-53	38.22852689149895	40.0	37.5	41.0	34.0	41.0
54-55	38.09946456953395	40.0	37.0	41.0	33.5	41.0
56-57	37.925126313453546	40.0	37.0	41.0	33.0	41.0
58-59	37.7263031403229	39.5	36.5	41.0	33.0	41.0
60-61	37.57910018835774	39.0	36.0	41.0	33.0	41.0
62-63	37.4264764216862	39.0	36.0	41.0	33.0	41.0
64-65	37.17905097348083	39.0	35.0	40.0	32.0	41.0
66-67	36.99141485753587	38.0	35.0	40.0	32.0	41.0
68-69	36.71590133236758	37.5	35.0	40.0	32.0	41.0
70-71	36.38729147500746	37.0	35.0	39.5	32.0	41.0
72-73	36.12177701679524	37.0	35.0	39.0	32.0	41.0
74-75	35.762494765515925	36.0	34.5	39.0	31.0	40.5
76-77	33.751343254166216	34.5	32.0	36.5	29.0	39.0
78-79	34.883160100951144	35.0	34.0	37.0	30.5	39.0
80-81	34.84064448471911	35.0	34.0	37.0	31.0	39.0
82-83	34.58316199948508	35.0	34.0	36.5	31.0	39.0
84-85	34.30498385595331	35.0	34.0	36.0	31.0	37.0
86-87	34.075396834414335	35.0	34.0	36.0	30.5	37.0
88-89	33.86552320036101	35.0	34.0	35.0	30.0	37.0
90-91	33.80224660326796	35.0	34.0	35.0	30.5	36.0
92-93	33.71359084028931	35.0	34.0	35.0	30.0	36.0
94-95	33.62655926813078	35.0	34.0	35.0	30.0	36.0
96-97	33.62363242953578	35.0	34.0	35.0	30.5	36.0
98-99	33.68717770360085	35.0	34.0	35.0	31.0	35.0
100-101	32.432839946669894	34.0	31.5	34.5	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	6.0
19	24.0
20	48.0
21	180.0
22	712.0
23	2244.0
24	6097.0
25	13350.0
26	24911.0
27	41406.0
28	62350.0
29	86754.0
30	116980.0
31	154334.0
32	203422.0
33	278694.0
34	412158.0
35	651761.0
36	1203407.0
37	2337619.0
38	2857593.0
39	1080610.0
40	17057.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.86175113856493	20.085059052733662	13.292018597284654	24.761171211416755
2	14.919200033586169	21.24185904467243	40.523575699904654	23.31536522183675
3	18.394229346780154	25.698589153683805	30.671828438273735	25.235353061262312
4	12.471880527510677	17.807301457182028	38.315611273648386	31.40520674165891
5	12.946091262963508	37.8340145546607	36.04092332300046	13.178970859375335
6	30.418318384467135	37.7992983846179	18.47513189313871	13.307251337776254
7	26.552482658353465	32.48128059070427	22.884953563846164	18.0812831870961
8	24.76630367414763	36.58514829592852	21.2974909628766	17.35105706704725
9	25.314326969564927	16.37504949392138	20.983653343423008	37.32697019309068
10-11	24.093863430818786	26.674963300899023	29.09288742291985	20.138285845362343
12-13	24.752830583254966	24.075762449554126	29.164077903430254	22.007329063760654
14-15	21.87862762193835	25.739008881339803	26.315078385947988	26.06728511077386
16-17	21.82698226908774	29.0387502512647	27.074163916714127	22.06010356293343
18-19	21.854756584601493	28.095938143896014	28.479460813171077	21.56984445833142
20-21	22.441825671019973	27.419123825828905	28.40085325350537	21.73819724964575
22-23	22.21023905108782	27.52105321411058	28.287490417506156	21.981217317295442
24-25	22.07493114136442	27.555467899888335	28.496823081408362	21.87277787733888
26-27	22.15728720378124	27.77561839982812	28.35138382945675	21.715710566933897
28-29	21.79061455120736	27.5970572005657	28.487761273063526	22.12456697516341
30-31	22.257992314088558	27.60857558827129	28.40142377274997	21.732008324890188
32-33	22.199529354555796	27.561050892387023	28.570057255810244	21.66936249724694
34-35	21.823101026394603	27.69426735587092	28.708747149050524	21.773884468683953
36-37	22.139693685585964	27.659417195132928	28.337561805816343	21.86332731346476
38-39	21.80506153184794	27.66218242457215	28.572407138865895	21.960348904714007
40-41	21.919420288361252	27.524964745611157	28.630284051487838	21.925330914539753
42-43	22.02836561446419	27.51363213876089	28.568787296047564	21.88921495072736
44-45	21.86898064197335	27.754484377474874	28.37559007546822	22.000944905083557
46-47	22.217979334719637	27.51995641037648	27.93707841801411	22.324985836889773
48-49	22.012776569813013	27.613863815719515	28.10691229236993	22.266447322097545
50-51	21.889756825075267	27.873295478048743	28.13322354630005	22.103724150575935
52-53	22.042229593785752	27.934609509534713	28.141793234044567	21.881367662634972
54-55	21.955950104126583	27.858873558180775	27.94811113326557	22.237065204427072
56-57	22.160564682542955	28.035158364451185	27.72236556126164	22.08191139174422
58-59	21.90377528770232	28.14968609564925	28.057328999261603	21.889209617386822
60-61	22.071641677915984	28.189838088891843	27.75522424774506	21.983295985447114
62-63	22.059543132843267	28.341070402181646	27.755575918562474	21.843810546412612
64-65	21.881782895775903	28.215850800516897	27.778717104668573	22.123649199038628
66-67	21.99654024897006	28.121782613328527	27.677523076536975	22.204154061164434
68-69	21.97540693511626	28.20523898272892	27.74321819179333	22.07613589036149
70-71	21.94432210983727	28.00385552530616	27.84259170463451	22.209230660222058
72-73	21.987297640197852	28.026545986728856	27.595747849245246	22.39040852382805
74-75	21.946722639184816	28.080506259786453	27.5888813069971	22.38388979403163
76-77	22.076006960348494	27.89270109101059	27.663751837182215	22.367540111458702
78-79	22.056580356403195	27.824875717932716	27.646100305755546	22.47244361990854
80-81	22.2091431172955	27.909435484752997	27.49153377755244	22.38988762039906
82-83	22.244925091513473	28.096354396806966	27.543972462494075	22.114748049185486
84-85	22.127508284842	27.871885029877618	27.518996425123294	22.481610260157087
86-87	22.029807753642938	28.07087684097488	27.55063685856157	22.34867854682061
88-89	22.277051522881475	27.869697923098478	27.531453756454745	22.3217967975653
90-91	22.29499552995416	28.059602498157865	27.594826904163376	22.050575067724598
92-93	22.11060327389752	28.02132529613216	27.603652142570883	22.26441928739943
94-95	22.220787725440545	27.909983521343072	27.624106829825884	22.245121923390496
96-97	22.213581806207745	28.100636012401154	27.684405993656043	22.00137618773506
98-99	22.473072332370457	28.502465308663254	27.828629144167373	21.195833214798917
100-101	23.071016603920288	31.244707895711976	24.953912953349345	20.73036254701839
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2137.0
1	1739.5
2	1973.5
3	2820.0
4	3817.5
5	5914.5
6	7764.5
7	9193.0
8	10225.0
9	10109.5
10	9110.0
11	8227.0
12	8980.0
13	12137.5
14	19042.5
15	27810.5
16	34837.5
17	39316.0
18	40913.0
19	39945.5
20	38130.0
21	36590.0
22	36797.5
23	40862.5
24	49660.0
25	62263.5
26	78661.0
27	97907.5
28	119334.0
29	142120.5
30	163303.5
31	184675.0
32	206468.5
33	227269.0
34	247458.0
35	265731.0
36	286154.5
37	304905.5
38	319237.5
39	332807.0
40	346870.5
41	361804.0
42	372844.5
43	381309.5
44	390960.5
45	399609.0
46	403975.0
47	403794.5
48	402073.5
49	405054.0
50	408761.0
51	397333.5
52	372569.5
53	353477.0
54	333579.0
55	305346.5
56	275937.5
57	246506.5
58	218023.5
59	188649.5
60	157793.0
61	126995.0
62	99342.0
63	76466.0
64	57329.5
65	42443.0
66	31924.0
67	24596.5
68	18760.0
69	13722.0
70	9955.0
71	7284.0
72	5252.5
73	3688.5
74	2592.5
75	1802.5
76	1274.0
77	893.0
78	608.5
79	418.0
80	289.0
81	201.0
82	152.5
83	114.0
84	90.0
85	67.5
86	45.5
87	34.0
88	21.0
89	11.5
90	9.0
91	7.0
92	6.5
93	5.0
94	3.0
95	2.0
96	1.0
97	1.0
98	0.5
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.001737907435909167
3	0.0022613735310625304
4	6.490979579901708E-4
5	0.0
6	4.711194856380272E-4
7	0.0
8	5.234660951533636E-5
9	4.083035542196236E-4
10-11	5.496393999110317E-4
12-13	2.041517771098118E-4
14-15	5.234660951533636E-5
16-17	3.8736491041348897E-4
18-19	0.0
20-21	9.946378029058186E-5
22-23	3.6649327869651534E-5
24-25	0.0
26-27	8.906443780640787E-5
28-29	2.8311191796842665E-4
30-31	7.403067852319612E-4
32-33	3.21025158583797E-4
34-35	0.0010346825593907788
36-37	1.8028818111284046E-4
38-39	2.0805946616955746E-4
40-41	2.8452524627404797E-4
42-43	0.001291668119117742
44-45	3.213582013744871E-4
46-47	1.9785446616886482E-4
48-49	3.2710856816540605E-4
50-51	0.0
52-53	4.843349464502951E-4
54-55	6.298282220125191E-4
56-57	6.754749361590319E-4
58-59	5.835478449433644E-4
60-61	7.207570832402355E-4
62-63	4.694939829064283E-5
64-65	7.170835252963956E-4
66-67	0.0025155965488650738
68-69	0.0013432767275310194
70-71	3.603852555030676E-4
72-73	0.0
74-75	7.461670332212217E-5
76-77	1.882599583870188E-5
78-79	9.563477901240942E-4
80-81	1.5350371264719008E-4
82-83	9.044101753380007E-5
84-85	8.350007254068802E-4
86-87	2.108711456049445E-4
88-89	3.996896543064195E-5
90-91	4.04345909838949E-5
92-93	9.553356691290443E-5
94-95	6.15439127227103E-4
96-97	1.4057073969447793E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	328.0
20-21	1068.0
22-23	2083.0
24-25	3480.0
26-27	5475.0
28-29	11313.0
30-31	21032.0
32-33	27098.0
34-35	37690.0
36-37	54395.0
38-39	58980.0
40-41	60867.0
42-43	68581.0
44-45	81054.0
46-47	80821.0
48-49	75358.0
50-51	68355.0
52-53	63688.0
54-55	74952.0
56-57	81179.0
58-59	50695.0
60-61	84018.0
62-63	82373.0
64-65	84013.0
66-67	89586.0
68-69	78334.0
70-71	75157.0
72-73	70681.0
74-75	73887.0
76-77	71694.0
78-79	76430.0
80-81	78280.0
82-83	75245.0
84-85	76220.0
86-87	80439.0
88-89	85274.0
90-91	91175.0
92-93	94379.0
94-95	108129.0
96-97	177500.0
98-99	850594.0
100-101	6119817.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.21468068059113
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.54205075770125	76.30297262802796
2	7.354100454021563	12.533546437648754
3	1.7895789247919673	4.574951898865893
4	0.621062354600527	2.1169452092007983
5	0.28398725470700426	1.2099941613607532
6	0.1476602847145137	0.7549694406691475
7	0.07736872949035194	0.46150661047283625
8	0.045398587984245245	0.3094900942741744
9	0.03584065835967783	0.2748735231751895
>10	0.10073601843784083	1.273102213565436
>50	0.0017675033386604366	0.09477309802906146
>100	4.0992065998548166E-4	0.06063115778740484
>500	3.8551192421121175E-5	0.03224352692256732
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.187728761226908E-5	0.0	0.0	0.0
3	0.0	4.187728761226908E-5	0.0	1.046932190306727E-5	0.0
4	0.0	8.375457522453817E-5	0.0	3.1407965709201814E-5	0.0
5	0.0	8.375457522453817E-5	0.0	4.187728761226908E-5	0.0
6	1.5703982854600905E-4	8.375457522453817E-5	0.0	7.32852533214709E-5	0.0
7	1.5703982854600905E-4	8.375457522453817E-5	0.0	9.422389712760543E-5	0.0
8	1.5703982854600905E-4	1.046932190306727E-4	0.0	9.422389712760543E-5	1.046932190306727E-5
9	1.5703982854600905E-4	2.512637256736145E-4	0.0	2.198557599644127E-4	4.187728761226908E-5
10-11	1.675091504490763E-4	2.512637256736145E-4	0.0	2.460290647220808E-4	4.187728761226908E-5
12-13	1.779784723521436E-4	2.564983866251481E-4	0.0	2.9837567423741723E-4	4.187728761226908E-5
14-15	1.779784723521436E-4	3.559569447042872E-4	0.0	4.501808418318926E-4	5.234660951533636E-5
16-17	3.088449961404845E-4	3.611916056558208E-4	0.0	6.17689992280969E-4	7.32852533214709E-5
18-19	3.140796570920181E-4	3.7689558851042174E-4	0.0	8.637190570030498E-4	9.422389712760543E-5
20-21	3.1931431804355174E-4	4.0306889326808995E-4	0.0	0.0011411560874343325	1.1516254093373997E-4
22-23	3.3501830089815266E-4	4.0830355421962353E-4	0.0	0.0015128170149932207	1.361011847398745E-4
24-25	3.4548762280121995E-4	4.4494618088035897E-4	0.0	0.002093864380613454	1.5703982854600905E-4
26-27	3.4548762280121995E-4	4.711194856380272E-4	0.0	0.002523106578639212	1.5703982854600905E-4
28-29	3.4548762280121995E-4	4.920581294441617E-4	0.0	0.003685201309879679	1.622744894975427E-4
30-31	3.4548762280121995E-4	5.60108721814099E-4	0.0	0.006689896696059986	1.779784723521436E-4
32-33	3.611916056558208E-4	5.758127046686999E-4	0.0	0.011008491981075234	2.198557599644127E-4
34-35	3.9259957136502266E-4	6.386286360871036E-4	0.0	0.016766619027762233	2.4079440377054723E-4
36-37	3.978342323165563E-4	7.014445675055072E-4	0.0	0.025047852653088447	2.4079440377054723E-4
38-39	3.978342323165563E-4	7.433218551177762E-4	0.0	0.04001898297447464	2.512637256736145E-4
40-41	3.978342323165563E-4	7.904338036815789E-4	0.0	0.07429030822416535	2.564983866251481E-4
42-43	4.292421980257581E-4	8.270764303423143E-4	0.0	0.0872932060277749	2.6173304757668174E-4
44-45	4.292421980257581E-4	9.265349884214535E-4	0.0	0.10510152258489233	2.6173304757668174E-4
46-47	4.292421980257581E-4	9.998202417429245E-4	0.0	0.12377355819901281	2.6173304757668174E-4
48-49	4.292421980257581E-4	0.001005054902694458	0.0	0.13937808249553457	2.72202369479749E-4
50-51	4.6588482468649353E-4	0.001120217443628198	0.0	0.15482033230255882	2.72202369479749E-4
52-53	4.711194856380272E-4	0.0011516254093373998	0.0	0.1769210708399338	2.72202369479749E-4
54-55	4.8158880754109446E-4	0.001162094731240467	0.0	0.2236875317809353	2.826716913828163E-4
56-57	4.920581294441617E-4	0.0011830333750466016	0.0	0.2744218657231993	2.9837567423741723E-4
58-59	5.234660951533635E-4	0.001193502696949669	0.0	0.3197016829539652	3.0361033518895087E-4
60-61	5.44404738959498E-4	0.0012039720188527362	0.0	0.6469988589486058	3.0361033518895087E-4
62-63	6.019860094263681E-4	0.0012249106626588705	0.0	0.6542279257226737	3.0361033518895087E-4
64-65	6.072206703779017E-4	0.0012981959159803415	0.0	0.6617134908833668	3.0361033518895087E-4
66-67	6.281593141840362E-4	0.0013138998988349426	0.0	0.6675763111490844	3.2454897899508544E-4
68-69	6.281593141840362E-4	0.0013296038816895434	0.0	0.6731145824358071	3.611916056558208E-4
70-71	6.386286360871035E-4	0.0013557771864472117	0.0	0.6783492433873408	3.6642626660735446E-4
72-73	7.014445675055072E-4	0.0013610118473987452	0.0	0.6823432896933609	3.87364910413489E-4
74-75	7.066792284570408E-4	0.0013871851521564133	0.0	0.6855207288909417	3.87364910413489E-4
76-77	7.223832113116416E-4	0.0014185931178656152	0.0	0.6884154963971398	4.0306889326808995E-4
78-79	7.223832113116416E-4	0.0014447664226232833	0.0	0.6910485308557613	4.187728761226908E-4
80-81	7.223832113116416E-4	0.001450001083574817	0.0	0.6924147773641116	4.344768589772917E-4
82-83	7.223832113116416E-4	0.001460470405477884	0.0	0.6929644167640227	4.868234684926281E-4
84-85	7.223832113116416E-4	0.0014918783711870861	0.0	0.6935768720953521	4.920581294441617E-4
86-87	7.276178722631753E-4	0.001507582354041687	0.0	0.6938124318381711	4.920581294441617E-4
88-89	7.328525332147089E-4	0.0015389903197508888	0.0	0.6938857170914926	5.129967732502962E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13770	0.0	30.759916	1
GTATCAA	19955	0.0	26.553934	1
TCAACGC	19540	0.0	25.932186	4
ATCAACG	19755	0.0	25.694302	3
CAACGCA	20075	0.0	25.369085	5
AACGCAG	20790	0.0	24.640945	6
GTACATG	24120	0.0	23.906012	1
TACATGG	23760	0.0	23.63671	2
CTTATAC	14310	0.0	23.487595	1
ACATGGG	23370	0.0	23.132284	3
TATCAAC	24045	0.0	21.573605	2
ACGCAGA	24370	0.0	20.932968	7
CGCAGAG	24630	0.0	20.7294	8
TATACAC	18795	0.0	20.323452	3
CATGGGG	16990	0.0	20.00903	4
TTATACA	18955	0.0	18.772255	2
GAGTACT	16095	0.0	17.458704	12-13
AGAGTAC	24145	0.0	16.911041	10-11
TACACAT	22640	0.0	16.852236	5
GCAGAGT	30590	0.0	16.788687	9
>>END_MODULE