FastQCFastQC Report
Fri 27 May 2016
SRR2139765_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139765_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9774361
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103150.047.3875161
GTATCAA176600.035.879531
TCAACGC206800.029.4089034
ATCAACG211300.028.865223
CAACGCA213750.028.593695
TATCAAC221400.027.8990042
AACGCAG221700.027.6849946
GTACATG281300.024.9921911
TACATGG288350.023.9027922
ACGCAGA263050.023.2836727
ACATGGG294550.022.711753
CGCAGAG270500.022.6105358
CATGGGG226600.020.5050684
GAGTACT172550.018.82211512-13
GCAGAGT324600.018.775729
AGAGTAC279800.017.69217310-11
CAGAGTA311550.017.44557210-11
GTGGTAT54150.016.8755341
GAGTACA201200.016.8603611
GTACTTT196200.016.5430714-15
Produced by FastQC (version 0.11.5)