##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139765_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9774361
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.534934406453786	34.0	31.0	34.0	31.0	34.0
2	32.733837229871085	34.0	31.0	34.0	31.0	34.0
3	32.827640599728205	34.0	31.0	34.0	31.0	34.0
4	36.23704475412766	37.0	37.0	37.0	35.0	37.0
5	36.078212273927676	37.0	35.0	37.0	35.0	37.0
6	36.09843538621092	37.0	36.0	37.0	35.0	37.0
7	36.121188280236424	37.0	36.0	37.0	35.0	37.0
8	36.12090826193139	37.0	36.0	37.0	35.0	37.0
9	38.00565029263806	39.0	38.0	39.0	35.0	39.0
10-11	37.89060220918789	39.0	38.0	39.0	35.0	39.0
12-13	37.92186803822777	39.0	38.0	39.0	35.0	39.0
14-15	39.269430093691035	40.0	39.0	41.0	36.0	41.0
16-17	39.23705396189071	40.0	39.0	41.0	36.0	41.0
18-19	39.26647608984362	40.0	39.0	41.0	36.0	41.0
20-21	39.307227384211885	40.0	39.0	41.0	36.0	41.0
22-23	39.26925381082779	40.0	39.0	41.0	36.0	41.0
24-25	39.260489999335675	40.0	39.0	41.0	36.0	41.0
26-27	39.16529870922594	40.0	39.0	41.0	36.0	41.0
28-29	39.093853782141366	40.0	39.0	41.0	36.0	41.0
30-31	38.98604439145211	40.0	38.5	41.0	35.0	41.0
32-33	38.91827729096131	40.0	38.0	41.0	35.0	41.0
34-35	38.860874167791735	40.0	38.0	41.0	35.0	41.0
36-37	38.7901210469465	40.0	38.0	41.0	35.0	41.0
38-39	38.697633110732745	40.0	38.0	41.0	35.0	41.0
40-41	38.68709261821424	40.0	38.0	41.0	35.0	41.0
42-43	38.62972365533079	40.0	38.0	41.0	35.0	41.0
44-45	38.592156442398064	40.0	38.0	41.0	35.0	41.0
46-47	38.48190756136519	40.0	38.0	41.0	34.0	41.0
48-49	38.45672544121582	40.0	38.0	41.0	34.0	41.0
50-51	38.34310595445431	40.0	38.0	41.0	34.0	41.0
52-53	38.17147018838263	40.0	37.0	41.0	34.0	41.0
54-55	38.0339776784767	40.0	37.0	41.0	33.5	41.0
56-57	37.8282374975351	39.5	36.5	41.0	33.0	41.0
58-59	37.61163646952494	39.0	36.0	41.0	33.0	41.0
60-61	37.44222359951189	39.0	36.0	41.0	33.0	41.0
62-63	37.25998434474465	39.0	35.0	40.5	33.0	41.0
64-65	36.999086481970046	38.5	35.0	40.0	32.0	41.0
66-67	36.78767587611637	38.0	35.0	40.0	32.0	41.0
68-69	36.497786800817764	37.0	35.0	40.0	32.0	41.0
70-71	36.15332177273713	37.0	35.0	39.0	32.0	41.0
72-73	35.89522487108128	36.0	35.0	39.0	31.0	41.0
74-75	35.538749538837976	36.0	34.5	39.0	31.0	40.0
76-77	33.55990738567284	34.5	32.0	36.5	28.0	39.0
78-79	34.651472320046636	35.0	34.0	37.0	30.5	39.0
80-81	34.61006157197903	35.0	34.0	36.5	31.0	39.0
82-83	34.369801956892914	35.0	34.0	36.0	31.0	37.5
84-85	34.1249897601469	35.0	34.0	36.0	31.0	37.0
86-87	33.92723369829581	35.0	34.0	35.0	30.0	37.0
88-89	33.74023727556946	35.0	34.0	35.0	30.0	36.0
90-91	33.71544453031291	35.0	34.0	35.0	30.0	36.0
92-93	33.6565010603241	35.0	34.0	35.0	30.0	36.0
94-95	33.58147441316032	35.0	34.0	35.0	30.0	36.0
96-97	33.572794653106605	35.0	34.0	35.0	30.0	35.0
98-99	33.64081994912523	35.0	34.0	35.0	31.0	35.0
100-101	32.34940165788632	34.0	31.5	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	5.0
19	10.0
20	55.0
21	169.0
22	659.0
23	2308.0
24	6287.0
25	13673.0
26	25229.0
27	41911.0
28	63183.0
29	87696.0
30	118211.0
31	155850.0
32	206435.0
33	284669.0
34	432921.0
35	694437.0
36	1312704.0
37	2540948.0
38	2821862.0
39	949819.0
40	15319.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.4333519091427	19.70054103792565	12.903595437082792	24.96251161584885
2	15.277145300024994	21.023823099436896	39.79934253234214	23.899689068195975
3	18.54799311775838	25.614363451478756	30.33281390149784	25.504829529265027
4	12.834532839275889	17.50475890759258	37.29614656882916	32.364561684302366
5	13.344648652187969	37.64225995359287	35.522786146612155	13.490305247607004
6	31.03080528596899	36.97972515555799	18.12474288516378	13.864726673309235
7	27.12704185982081	32.12233515827786	22.346985137954288	18.40363784394704
8	25.435179565794414	35.85117014358798	21.052220729427084	17.661429561190527
9	25.79663853853772	15.957560219022909	20.38341286145737	37.862388380982004
10-11	24.72331199664345	26.12976767748022	28.562601506406356	20.584318819469978
12-13	25.3460768274341	23.55627951411997	28.60732282166778	22.49032083677815
14-15	22.471758373888	25.05502279817369	25.664755969893672	26.808462858044635
16-17	22.457808023501606	28.461003542858414	26.37804342423727	22.703145009402707
18-19	22.66437673010031	27.449277758413054	27.828647826696805	22.05769768478983
20-21	23.04686632739056	26.637907899439195	27.79224196734441	22.522983805825834
22-23	22.76420863356281	26.970834626648564	27.63449911365592	22.630457626132706
24-25	22.75606452416161	26.997154194688168	27.73014185836189	22.516639422788334
26-27	22.73724937656588	27.1091235348959	27.795503881850408	22.358123206687807
28-29	22.633741335008725	26.867378851250262	27.8933474272194	22.605532386521617
30-31	22.526088511818067	26.682315745515478	28.037852543660367	22.753743199006085
32-33	22.28425384986329	27.13211532789326	27.90281598482553	22.680814837417916
34-35	22.691840306413578	26.90245606948863	27.922113824991207	22.48358979910659
36-37	22.50028429146474	27.023096917657984	27.987949984623363	22.488668806253916
38-39	22.46180638458005	26.91116909843704	28.001852001554962	22.62517251542795
40-41	22.519035839864475	26.677735172260697	28.08342524158503	22.719803746289795
42-43	22.820586874873385	26.71321369351456	27.94037939520251	22.525820036409545
44-45	22.382980709336717	26.972453965099376	27.83931167091202	22.805253654651885
46-47	22.868619703056474	26.698310538239657	27.384505632587985	23.048564126115885
48-49	22.57935155033241	26.99643799460122	27.44086497300203	22.983345482064344
50-51	22.37591344525284	27.16034332162667	27.630365413135742	22.833377819984747
52-53	22.551961086563356	27.09593516072639	27.555148707531536	22.796955045178716
54-55	22.42641738250973	26.888239950572363	27.430988026249103	23.254354640668808
56-57	22.68439436440166	26.863071506445596	27.376369935625917	23.07616419352683
58-59	22.22742365467588	26.883888769194908	27.896239061016725	22.992448515112486
60-61	22.579625968234076	26.917700444866526	27.611385144313594	22.891288442585804
62-63	22.179954471175332	27.565323451401326	27.607343297749992	22.647378779673346
64-65	21.909316665158794	27.592419058618532	27.44927568598862	23.04898859023405
66-67	21.999463375121376	27.88567202924415	27.094649130308447	23.020215465326025
68-69	22.112188465029085	27.999993933248703	26.79616963989981	23.0916479618224
70-71	22.118093267659166	27.678871380649824	26.781765021928454	23.42127032976256
72-73	22.028388720350954	27.34314774376907	26.694629456632878	23.93383407924709
74-75	22.355514550130735	27.06292957674728	26.8013057282827	23.780250144839286
76-77	22.798222194610936	26.89868598733928	26.702238710257497	23.600853107792283
78-79	22.943239610304545	26.771336568745273	26.09332402487598	24.1920997960742
80-81	22.825931615328297	27.181517492958335	26.03941996276808	23.953130928945292
82-83	22.7548470514361	27.33385746461848	26.132683982941344	23.77861150100407
84-85	22.78841779573168	27.310695022671926	26.099047061438185	23.801840120158207
86-87	23.083034272906445	27.540190232210797	26.040176237218066	23.336599257664687
88-89	23.179679385579842	27.297878555167383	26.381609279801378	23.140832779451397
90-91	22.982804148431235	27.33747019804208	26.57021963772307	23.109506015803618
92-93	22.77904226872474	26.870392859045843	26.50021114612982	23.850353726099595
94-95	22.70086932985926	26.87981310086697	27.091813634664824	23.327503934608956
96-97	22.395356391398302	27.06038574295018	27.238140386469954	23.30611747918156
98-99	22.628507416765764	27.284580247398033	27.58047033037777	22.506442005458428
100-101	23.61245406607084	30.01360548413659	24.651548163527465	21.722392286265098
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2452.0
1	2111.0
2	2557.5
3	3552.5
4	4625.5
5	7121.5
6	9424.0
7	11146.5
8	12367.5
9	12356.0
10	11076.5
11	9608.0
12	10139.5
13	13329.0
14	20531.0
15	29954.0
16	37456.0
17	41724.5
18	42544.0
19	40587.5
20	37584.0
21	34719.0
22	33498.5
23	35947.5
24	42274.0
25	52451.0
26	65829.0
27	80167.0
28	97793.0
29	118722.5
30	137804.5
31	156956.0
32	177292.5
33	195711.5
34	211765.5
35	227073.5
36	245576.0
37	264543.0
38	282622.0
39	298819.0
40	313351.5
41	330120.5
42	343343.0
43	355039.0
44	369530.5
45	382720.0
46	394298.5
47	407063.5
48	432249.0
49	445181.5
50	438725.5
51	436161.5
52	425985.0
53	407634.5
54	386858.0
55	362751.0
56	334738.5
57	303111.5
58	270483.5
59	234513.5
60	196480.5
61	161535.0
62	129475.5
63	100955.0
64	75787.5
65	56377.5
66	43066.0
67	33006.0
68	25308.0
69	18713.5
70	13368.0
71	9627.5
72	6973.5
73	4948.0
74	3488.5
75	2417.5
76	1626.0
77	1077.0
78	727.0
79	510.5
80	360.5
81	256.0
82	190.5
83	126.5
84	81.5
85	58.5
86	38.5
87	27.0
88	18.0
89	12.0
90	10.0
91	8.0
92	6.0
93	4.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.001964322782839717
3	0.0022507865220038423
4	6.036200218101214E-4
5	1.0230847827290194E-5
6	3.273871304732862E-4
7	0.0
8	5.115423913645096E-5
9	4.1946476091889786E-4
10-11	4.041184891779626E-4
12-13	1.432318695820627E-4
14-15	3.5807967395515675E-5
16-17	5.115423913645097E-4
18-19	0.0
20-21	1.0742833698632308E-4
22-23	2.0464519000666377E-5
24-25	0.0
26-27	1.1262782682406781E-4
28-29	3.432606358539525E-4
30-31	8.107733510294026E-4
32-33	3.9615297390494056E-4
34-35	0.001099602625293524
36-37	2.178871442123416E-4
38-39	2.193319837105269E-4
40-41	3.252141010316421E-4
42-43	0.0014001849617915941
44-45	4.688229539127857E-4
46-47	2.4202646500232774E-4
48-49	3.528176615527646E-4
50-51	0.0
52-53	4.517520542322832E-4
54-55	7.782342130636382E-4
56-57	5.588380992355925E-4
58-59	7.213028416058354E-4
60-61	7.582292826253377E-4
62-63	6.761032653083301E-5
64-65	8.033056082752556E-4
66-67	0.0026582580371653304
68-69	0.0015574974690666127
70-71	3.241576865655676E-4
72-73	0.0
74-75	9.01114624723906E-5
76-77	6.066932216289774E-6
78-79	0.0010783773215288597
80-81	1.424183263406967E-4
82-83	1.501926126386708E-4
84-85	9.863447524024924E-4
86-87	2.683964005230918E-4
88-89	4.524561062635826E-5
90-91	3.925289956262457E-5
92-93	1.6568250156188895E-4
94-95	8.06334672700021E-4
96-97	2.0498321016689392E-5
98-99	7.108811521165527E-6
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	270.0
20-21	758.0
22-23	1678.0
24-25	3781.0
26-27	5980.0
28-29	12589.0
30-31	23887.0
32-33	30690.0
34-35	42034.0
36-37	60312.0
38-39	43907.0
40-41	67950.0
42-43	73907.0
44-45	87658.0
46-47	86997.0
48-49	79760.0
50-51	72721.0
52-53	15387.0
54-55	21296.0
56-57	24775.0
58-59	25686.0
60-61	95405.0
62-63	96814.0
64-65	99564.0
66-67	106251.0
68-69	89557.0
70-71	83106.0
72-73	78708.0
74-75	82188.0
76-77	79340.0
78-79	85058.0
80-81	85914.0
82-83	81866.0
84-85	82956.0
86-87	87275.0
88-89	91847.0
90-91	97178.0
92-93	101183.0
94-95	114595.0
96-97	185012.0
98-99	922005.0
100-101	6246516.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.46447409440916
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.67418710699182	71.42331544325916
2	8.087469584918008	13.176829129797499
3	2.2647558912029764	5.5349144288719625
4	0.8910619178157428	2.9035956208166045
5	0.4093355415913007	1.667315231194273
6	0.2217918386987665	1.0840893298816223
7	0.13603363485677902	0.7757335965930873
8	0.07144602431728983	0.4656250237715507
9	0.05755183387074434	0.4219586891504092
>10	0.18220890449580374	2.266286757107552
>50	0.003522508731141289	0.1972754384660559
>100	6.352125096338697E-4	0.08306131109027323
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.0461695654580388E-5	0.0	0.0	0.0
3	0.0	2.0461695654580388E-5	0.0	0.0	0.0
4	0.0	7.161593479103135E-5	0.0	2.0461695654580388E-5	0.0
5	0.0	7.161593479103135E-5	0.0	3.069254348187058E-5	0.0
6	1.0230847827290194E-5	7.161593479103135E-5	0.0	3.069254348187058E-5	1.0230847827290194E-5
7	1.0230847827290194E-5	7.161593479103135E-5	0.0	3.069254348187058E-5	1.0230847827290194E-5
8	2.0461695654580388E-5	8.184678261832155E-5	0.0	3.069254348187058E-5	1.0230847827290194E-5
9	2.0461695654580388E-5	1.9438610871851366E-4	0.0	4.0923391309160775E-5	1.0230847827290194E-5
10-11	3.069254348187058E-5	1.9438610871851366E-4	0.0	4.603881522280587E-5	1.534627174093529E-5
12-13	4.0923391309160775E-5	1.9438610871851366E-4	0.0	5.626966305009607E-5	2.0461695654580388E-5
14-15	5.115423913645097E-5	2.66002043509545E-4	0.0	6.650051087738626E-5	5.626966305009606E-5
16-17	5.115423913645097E-5	2.66002043509545E-4	0.0	8.184678261832155E-5	7.161593479103135E-5
18-19	5.115423913645097E-5	2.7111746742319013E-4	0.0	9.719305435925684E-5	7.673135870467644E-5
20-21	5.115423913645097E-5	2.7623289133683524E-4	0.0	2.1484780437309406E-4	8.696220653196664E-5
22-23	8.184678261832155E-5	3.018100109050607E-4	0.0	4.0411848917796265E-4	9.207763044561174E-5
24-25	8.696220653196664E-5	3.222717065596411E-4	0.0	5.626966305009606E-4	9.207763044561174E-5
26-27	9.207763044561174E-5	3.273871304732862E-4	0.0	8.696220653196665E-4	9.207763044561174E-5
28-29	9.207763044561174E-5	3.273871304732862E-4	0.0	0.0031408702829780893	9.207763044561174E-5
30-31	1.1253932610019213E-4	3.6831052178244695E-4	0.0	0.008962222696706209	9.207763044561174E-5
32-33	1.278855978411274E-4	3.6831052178244695E-4	0.0	0.019054954078327982	9.207763044561174E-5
34-35	1.483472934957078E-4	4.194647609188979E-4	0.0	0.03193559149288634	1.0230847827290194E-4
36-37	1.636935652366431E-4	4.706190000553489E-4	0.0	0.04913364669056115	1.0230847827290194E-4
38-39	1.636935652366431E-4	4.910806957099293E-4	0.0	0.09031792461931781	1.1253932610019213E-4
40-41	1.739244130639333E-4	5.524657826736705E-4	0.0	0.39406668118765	1.2277017392748233E-4
42-43	1.739244130639333E-4	5.678120544146057E-4	0.0	0.4295165689092105	1.2277017392748233E-4
44-45	1.739244130639333E-4	6.496588370329272E-4	0.0	0.4877710164378009	1.2277017392748233E-4
46-47	1.7903983697757837E-4	7.161593479103135E-4	0.0	0.5538111391629591	1.330010217547725E-4
48-49	1.8415526089122348E-4	7.161593479103135E-4	0.0	0.6057940769734206	1.432318695820627E-4
50-51	1.8415526089122348E-4	7.980061305286351E-4	0.0	0.6557052680988558	1.534627174093529E-4
52-53	1.9950153263215877E-4	8.082369783559253E-4	0.0	0.8452573012189748	1.7392441306393326E-4
54-55	2.0461695654580388E-4	8.133524022695703E-4	0.0	1.4874629656097211	1.9438610871851366E-4
56-57	2.0461695654580388E-4	8.184678261832155E-4	0.0	2.187662190909462	1.9438610871851366E-4
58-59	2.0461695654580388E-4	8.184678261832155E-4	0.0	2.9016167911129944	1.9438610871851366E-4
60-61	2.0461695654580388E-4	8.184678261832155E-4	0.0	3.440485777024196	2.0461695654580388E-4
62-63	2.0461695654580388E-4	8.286986740105057E-4	0.0	3.4545531927867206	2.0461695654580388E-4
64-65	2.0461695654580388E-4	9.51468847937988E-4	0.0	3.462277482896324	2.0461695654580388E-4
66-67	2.0461695654580388E-4	9.616996957652782E-4	0.0	3.4681755666687573	2.0973238045944896E-4
68-69	2.0461695654580388E-4	9.821613914198586E-4	0.0	3.473797417549853	2.1484780437309406E-4
70-71	2.1484780437309406E-4	0.0010179693588153742	0.0	3.479025380789598	2.1484780437309406E-4
72-73	2.1484780437309406E-4	0.0010384310544699546	0.0	3.482667562616114	2.2507865220038425E-4
74-75	2.1484780437309406E-4	0.0010691235979518252	0.0	3.4859874727360696	2.2507865220038425E-4
76-77	2.1484780437309406E-4	0.001099816141433696	0.0	3.4888725718233653	2.2507865220038425E-4
78-79	2.1484780437309406E-4	0.001115162413174631	0.0	3.4916297853128198	2.2507865220038425E-4
80-81	2.1484780437309406E-4	0.001115162413174631	0.0	3.492816563660786	2.3530950002767444E-4
82-83	2.1484780437309406E-4	0.001115162413174631	0.0	3.493333221476064	2.4554034785496465E-4
84-85	2.1484780437309406E-4	0.0011356241088292115	0.0	3.493788494204378	2.4554034785496465E-4
86-87	2.2507865220038425E-4	0.001156085804483792	0.0	3.494049380823974	2.4554034785496465E-4
88-89	2.2507865220038425E-4	0.001278855978411274	0.0	3.4941619201500744	2.4554034785496465E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10315	0.0	47.387516	1
GTATCAA	17660	0.0	35.87953	1
TCAACGC	20680	0.0	29.408903	4
ATCAACG	21130	0.0	28.86522	3
CAACGCA	21375	0.0	28.59369	5
TATCAAC	22140	0.0	27.899004	2
AACGCAG	22170	0.0	27.684994	6
GTACATG	28130	0.0	24.992191	1
TACATGG	28835	0.0	23.902792	2
ACGCAGA	26305	0.0	23.283672	7
ACATGGG	29455	0.0	22.71175	3
CGCAGAG	27050	0.0	22.610535	8
CATGGGG	22660	0.0	20.505068	4
GAGTACT	17255	0.0	18.822115	12-13
GCAGAGT	32460	0.0	18.77572	9
AGAGTAC	27980	0.0	17.692173	10-11
CAGAGTA	31155	0.0	17.445572	10-11
GTGGTAT	5415	0.0	16.875534	1
GAGTACA	20120	0.0	16.860361	1
GTACTTT	19620	0.0	16.54307	14-15
>>END_MODULE