FastQCFastQC Report
Fri 27 May 2016
SRR2139764_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139764_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12057465
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA126600.039.4779431
GTATCAA202950.031.860541
TCAACGC212650.029.285734
ATCAACG218150.028.5675853
CAACGCA221650.028.1944055
AACGCAG229400.027.279656
TATCAAC252450.025.2187942
GTACATG282900.022.8564761
ACGCAGA275050.022.7362147
CGCAGAG282750.022.1170488
TACATGG284600.022.0960222
CTTATAC141250.021.6777061
ACATGGG293150.020.9485683
TATACAC184000.018.9237063
CATGGGG219150.018.5560994
GCAGAGT353800.017.6632679
GAGTACT198650.017.42965912-13
TTATACA185250.017.0432222
AGAGTAC297350.016.86565210-11
CAGAGTA328800.016.5629910-11
Produced by FastQC (version 0.11.5)