##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139764_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12057465
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.54975585664151	34.0	31.0	34.0	31.0	34.0
2	32.73360096836275	34.0	31.0	34.0	31.0	34.0
3	32.82366691506051	34.0	31.0	34.0	31.0	34.0
4	36.22971702592543	37.0	37.0	37.0	35.0	37.0
5	36.0843106739269	37.0	35.0	37.0	35.0	37.0
6	36.106289671999875	37.0	36.0	37.0	35.0	37.0
7	36.12422279475827	37.0	36.0	37.0	35.0	37.0
8	36.1197345378983	37.0	36.0	37.0	35.0	37.0
9	37.986997847391635	39.0	38.0	39.0	35.0	39.0
10-11	37.8733960247863	39.0	38.0	39.0	35.0	39.0
12-13	37.905211833498996	39.0	38.0	39.0	35.0	39.0
14-15	39.26229738174649	40.0	39.0	41.0	36.0	41.0
16-17	39.23200797182493	40.0	39.0	41.0	36.0	41.0
18-19	39.25598573166084	40.0	39.0	41.0	36.0	41.0
20-21	39.29347278489564	40.0	39.0	41.0	36.0	41.0
22-23	39.256713290072355	40.0	39.0	41.0	36.0	41.0
24-25	39.24860241263501	40.0	39.0	41.0	36.0	41.0
26-27	39.15376374612348	40.0	39.0	41.0	36.0	41.0
28-29	39.07509648342398	40.0	39.0	41.0	36.0	41.0
30-31	38.96846284931948	40.0	38.5	41.0	35.0	41.0
32-33	38.91309925078032	40.0	38.0	41.0	35.0	41.0
34-35	38.85377661478547	40.0	38.0	41.0	35.0	41.0
36-37	38.79274281737331	40.0	38.0	41.0	35.0	41.0
38-39	38.70392622434265	40.0	38.0	41.0	35.0	41.0
40-41	38.692842432132764	40.0	38.0	41.0	35.0	41.0
42-43	38.6406223812312	40.0	38.0	41.0	35.0	41.0
44-45	38.61485951817058	40.0	38.0	41.0	35.0	41.0
46-47	38.51412168319744	40.0	38.0	41.0	34.0	41.0
48-49	38.50192718228365	40.0	38.0	41.0	34.0	41.0
50-51	38.421387237452635	40.0	38.0	41.0	34.0	41.0
52-53	38.26029943066135	40.0	38.0	41.0	34.0	41.0
54-55	38.12214500224243	40.0	37.0	41.0	33.5	41.0
56-57	37.93232182380805	40.0	37.0	41.0	33.0	41.0
58-59	37.72419554131821	39.5	36.5	41.0	33.0	41.0
60-61	37.56347411369008	39.0	36.0	41.0	33.0	41.0
62-63	37.39541327922066	39.0	36.0	41.0	33.0	41.0
64-65	37.13479867577078	39.0	35.0	40.0	32.0	41.0
66-67	36.933298744507226	38.0	35.0	40.0	32.0	41.0
68-69	36.65140208101649	37.5	35.0	40.0	32.0	41.0
70-71	36.316944822249184	37.0	35.0	39.5	32.0	41.0
72-73	36.05016322816434	36.5	35.0	39.0	31.0	41.0
74-75	35.68943286072182	36.0	34.5	39.0	31.0	40.5
76-77	33.687589256676304	34.5	32.0	36.5	28.5	39.0
78-79	34.81395923870606	35.0	34.0	37.0	30.5	39.0
80-81	34.77648710038967	35.0	34.0	37.0	31.0	39.0
82-83	34.529481052428636	35.0	34.0	36.5	31.0	38.5
84-85	34.25619182490291	35.0	34.0	36.0	31.0	37.0
86-87	34.03769210386814	35.0	34.0	36.0	30.5	37.0
88-89	33.83233920851079	35.0	34.0	35.0	30.0	36.5
90-91	33.77645655371349	35.0	34.0	35.0	30.0	36.0
92-93	33.69572817419636	35.0	34.0	35.0	30.0	36.0
94-95	33.61240542343196	35.0	34.0	35.0	30.0	36.0
96-97	33.61505964246446	35.0	34.0	35.0	30.5	35.5
98-99	33.67400717678764	35.0	34.0	35.0	31.0	35.0
100-101	32.416584340878146	34.0	31.5	34.5	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	9.0
19	33.0
20	58.0
21	201.0
22	796.0
23	2871.0
24	7737.0
25	16759.0
26	31744.0
27	52577.0
28	79037.0
29	109518.0
30	147882.0
31	193746.0
32	256810.0
33	351172.0
34	520205.0
35	822776.0
36	1530726.0
37	3000744.0
38	3612314.0
39	1300159.0
40	19591.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.3826898937712	19.780509418853796	13.061999350609769	24.774801336765233
2	15.140288394502422	21.230507759905795	40.12235310353934	23.50685074205245
3	18.539386974603687	25.480294569610944	30.416107110914126	25.56421134487124
4	12.649178098097433	17.577562326869806	38.26231194121452	31.510947633818237
5	13.182763805058842	37.70405617632365	35.886128293644504	13.227051724973013
6	30.77631418748899	37.583678819302186	18.40462945116249	13.235377542046336
7	26.90251226107644	32.23102036787998	22.859738759349497	18.006728611694083
8	24.9116252437599	36.4115606779173	21.237302154624786	17.43951192369802
9	25.527859827334463	16.27421798121737	20.841175326552168	37.356746864896
10-11	24.283301598301886	26.480891532873553	29.064062410519092	20.17174445830547
12-13	24.91652673246995	23.944161341537058	29.123198738572732	22.01611318742026
14-15	22.011345422915095	25.532397511698463	26.25631025236946	26.199946813016982
16-17	22.020736282074342	28.746438810236057	27.03081016439922	22.202014743290373
18-19	22.037057540701962	27.98319961948884	28.39703038739901	21.58271245241019
20-21	22.593415919244762	27.247702495641867	28.31849437718767	21.840387207925698
22-23	22.297968302169263	27.371850108532854	28.18169066378573	22.148490925512153
24-25	22.20855329501535	27.487675148629187	28.254899117011455	22.04887243934401
26-27	22.25215654535076	27.63225028318314	28.182738224731587	21.932854946734512
28-29	22.055184744925572	27.52097897494799	28.25525088492251	22.168585395203923
30-31	22.421443731521205	27.48854147338326	28.187888148778327	21.902126646317207
32-33	22.228359608375307	27.60824394601312	28.22953968686995	21.93385675874163
34-35	22.27657231948033	27.64314312559293	28.238012411955832	21.842272142970913
36-37	22.294616323781586	27.502774055745665	28.13297707579336	22.069632544679386
38-39	22.041563724828876	27.50076031409802	28.354784944519118	22.10289101655398
40-41	22.217546419671123	27.382604560276842	28.298109347066045	22.101739672985985
42-43	22.191465392482257	27.460485773554883	28.209497504269137	22.13855132969372
44-45	22.128235945588763	27.60457806593573	28.08039326557934	22.186792722896165
46-47	22.377783241247098	27.481248269427795	27.724915437111548	22.416053052213563
48-49	22.188353282765842	27.602660429174197	27.83796878399479	22.371017504065176
50-51	22.166736262483298	27.746129247022548	27.841319073737992	22.245815416756166
52-53	22.30784757364546	27.6857232165609	27.81227876752469	22.19415044226895
54-55	22.193458520961208	27.64199417769076	27.748023736249667	22.416523565098366
56-57	22.320320303689495	27.801769321801885	27.57794470302466	22.29996567148396
58-59	22.157873071235574	27.845319372677306	27.81147177456486	22.185335781522262
60-61	22.335859245273294	27.970262854635504	27.53548094003424	22.15839696005696
62-63	22.218312923530025	28.115040224476807	27.528636225903274	22.138010626089894
64-65	22.213266472663193	27.982840495448137	27.51371423181646	22.290178800072212
66-67	22.24964338843491	27.94891843609185	27.442785128723667	22.358653046749566
68-69	22.256866113019782	27.90106042015248	27.483279539802126	22.358793927025612
70-71	22.286834054735717	27.709589588962686	27.52679328570157	22.476783070600035
72-73	22.261692196067102	27.724758059818665	27.479274505244817	22.534275238869416
74-75	22.168804348961686	27.840182468180473	27.465734978472266	22.525278204385565
76-77	22.380365033158654	27.766511743728955	27.401974083575283	22.451149139537108
78-79	22.302911843890442	27.75285695535354	27.36395311948991	22.580278081266115
80-81	22.318925802536985	27.812149702282447	27.35565160256345	22.513272892617117
82-83	22.33358059116785	27.80689376738031	27.41607585795598	22.44344978349586
84-85	22.414186962138793	27.766335668938012	27.424739465960158	22.394737902963037
86-87	22.325987746995697	27.757149009055244	27.46818027647948	22.44868296746958
88-89	22.35756226452905	27.767569992581915	27.471428370619684	22.403439372269354
90-91	22.356204405238845	27.848689880196076	27.4620579099227	22.333047804642383
92-93	22.391289082433016	27.785315723597005	27.499879192837607	22.323516001132376
94-95	22.332747263336664	27.843617380077372	27.497385176970113	22.32625017961585
96-97	22.334746160694202	27.829279495940295	27.55826999339285	22.277704349972648
98-99	22.58099347804958	28.40735532635314	27.635670782235174	21.375980413362107
100-101	23.38568304077778	30.980081241912288	24.738963882871662	20.89527183443827
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2675.0
1	2140.0
2	2369.5
3	3340.0
4	4444.0
5	7041.0
6	9383.5
7	11158.0
8	12545.0
9	12553.5
10	11345.0
11	10194.0
12	10960.5
13	14414.5
14	22240.5
15	32323.5
16	40476.0
17	45690.5
18	47618.5
19	46322.0
20	43864.0
21	41980.0
22	42506.5
23	47705.0
24	57736.0
25	72563.5
26	92035.0
27	114115.5
28	139195.5
29	166777.5
30	192677.5
31	219993.0
32	248324.5
33	274154.5
34	298685.5
35	321815.0
36	347795.0
37	372206.5
38	392181.5
39	410856.5
40	429089.0
41	448325.0
42	463791.5
43	475646.0
44	486562.0
45	497205.5
46	505696.5
47	510712.0
48	520133.5
49	519379.0
50	507133.0
51	494210.0
52	472868.5
53	444774.5
54	414962.0
55	382987.5
56	350057.0
57	317646.5
58	282970.0
59	245051.0
60	205320.5
61	168582.0
62	135260.0
63	105217.5
64	79098.5
65	59140.0
66	45056.5
67	34635.5
68	26239.5
69	19157.5
70	13962.0
71	10273.5
72	7558.0
73	5445.5
74	3810.0
75	2653.5
76	1841.5
77	1253.0
78	850.0
79	589.0
80	419.5
81	300.5
82	213.5
83	137.0
84	86.5
85	56.5
86	43.0
87	37.0
88	29.5
89	20.5
90	10.0
91	5.0
92	4.0
93	3.5
94	3.0
95	2.5
96	2.5
97	3.0
98	1.5
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0019158255902049061
3	0.0023305064538856218
4	5.390851227849304E-4
5	8.293617273614313E-6
6	4.395617155015586E-4
7	0.0
8	4.976170364168588E-5
9	4.1468086368071566E-4
10-11	6.178744868842662E-4
12-13	9.952340728337176E-5
14-15	2.9027660457650093E-5
16-17	3.9809362913348703E-4
18-19	0.0
20-21	6.635267475096598E-5
22-23	8.295081257371734E-6
24-25	0.0
26-27	7.470202606835103E-5
28-29	2.2425918780548303E-4
30-31	5.863444372692334E-4
32-33	3.0006143757934435E-4
34-35	9.737952126221246E-4
36-37	1.0911141135400757E-4
38-39	1.0971635831657802E-4
40-41	2.631192979501818E-4
42-43	0.0013620072725210892
44-45	3.998647855077995E-4
46-47	1.387056577517651E-4
48-49	3.669785051951924E-4
50-51	0.0
52-53	4.783681268632273E-4
54-55	7.132182112959768E-4
56-57	6.870146331422684E-4
58-59	6.380322037025959E-4
60-61	6.613684214367294E-4
62-63	8.275262825795374E-5
64-65	6.674280599456074E-4
66-67	0.0026095414656840386
68-69	0.0012268370221098209
70-71	1.7608110467003257E-4
72-73	0.0
74-75	1.2575723996522523E-4
76-77	0.0
78-79	9.786060970798885E-4
80-81	1.5380640080717598E-4
82-83	1.251641089214123E-4
84-85	8.385757790621571E-4
86-87	2.447067009208466E-4
88-89	2.0595779255467728E-5
90-91	3.122237307832148E-5
92-93	1.210702122481891E-4
94-95	5.169925043015642E-4
96-97	1.6240429379629674E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	404.0
20-21	1252.0
22-23	2312.0
24-25	4271.0
26-27	6863.0
28-29	13103.0
30-31	24688.0
32-33	31423.0
34-35	43762.0
36-37	62742.0
38-39	67973.0
40-41	69673.0
42-43	77600.0
44-45	92519.0
46-47	92332.0
48-49	85115.0
50-51	76286.0
52-53	69188.0
54-55	79467.0
56-57	85072.0
58-59	87096.0
60-61	88381.0
62-63	86055.0
64-65	92312.0
66-67	98146.0
68-69	91017.0
70-71	86996.0
72-73	82973.0
74-75	85972.0
76-77	82741.0
78-79	89272.0
80-81	91287.0
82-83	89402.0
84-85	88883.0
86-87	95182.0
88-89	100851.0
90-91	108375.0
92-93	114023.0
94-95	133756.0
96-97	224011.0
98-99	1070412.0
100-101	7984277.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.92612497918776
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.90185773721714	74.61188423335655
2	7.638587726467601	12.821541363920192
3	1.9448543824747568	4.8967227590969244
4	0.7168505582408242	2.4064995816927968
5	0.313268098071767	1.314568877538178
6	0.16784862037285803	0.8452130574598035
7	0.09388855335433913	0.5515791724051983
8	0.05505262606778507	0.3696282860637936
9	0.04329531259526805	0.3270247034295121
>10	0.12111033091066142	1.5902614532619184
>50	0.0027280051223141544	0.15677521587196724
>100	6.411531286316364E-4	0.09492942580702983
>500	1.6895976030565546E-5	0.013371870096153685
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.293617273614313E-6
2	0.0	1.6587234547228626E-5	0.0	8.293617273614313E-6	8.293617273614313E-6
3	0.0	1.6587234547228626E-5	0.0	8.293617273614313E-6	8.293617273614313E-6
4	0.0	2.488085182084294E-5	0.0	8.293617273614313E-6	8.293617273614313E-6
5	0.0	3.317446909445725E-5	0.0	8.293617273614313E-6	8.293617273614313E-6
6	7.464255546252882E-5	3.317446909445725E-5	0.0	8.293617273614313E-6	8.293617273614313E-6
7	7.464255546252882E-5	3.317446909445725E-5	0.0	2.488085182084294E-5	8.293617273614313E-6
8	7.464255546252882E-5	5.805532091530019E-5	0.0	2.488085182084294E-5	8.293617273614313E-6
9	7.464255546252882E-5	1.32697876377829E-4	0.0	4.976170364168588E-5	8.293617273614313E-6
10-11	7.464255546252882E-5	1.32697876377829E-4	0.0	9.952340728337176E-5	8.293617273614313E-6
12-13	7.464255546252882E-5	1.32697876377829E-4	0.0	1.1611064183060038E-4	8.293617273614313E-6
14-15	8.293617273614313E-5	1.5757872819867195E-4	0.0	1.7416596274590058E-4	1.6587234547228626E-5
16-17	1.5757872819867195E-4	1.5757872819867195E-4	0.0	2.5295532684523655E-4	2.488085182084294E-5
18-19	1.5757872819867195E-4	1.617255368354791E-4	0.0	4.105340550439085E-4	3.317446909445725E-5
20-21	1.5757872819867195E-4	1.6587234547228626E-4	0.0	6.842234250731808E-4	3.317446909445725E-5
22-23	1.617255368354791E-4	1.7416596274590058E-4	0.0	0.0010698766282962464	3.317446909445725E-5
24-25	1.6587234547228626E-4	1.9904681456674352E-4	0.0	0.0013394191896887115	3.317446909445725E-5
26-27	1.6587234547228626E-4	2.0734043184035783E-4	0.0	0.001613108559717984	3.317446909445725E-5
28-29	1.6587234547228626E-4	2.0734043184035783E-4	0.0	0.002836417107576095	3.732127773126441E-5
30-31	1.7001915410909342E-4	2.2392766638758646E-4	0.0	0.006527076794334464	4.1468086368071566E-5
32-33	1.824595800195149E-4	2.280744750243936E-4	0.0	0.012917308903654293	4.976170364168588E-5
34-35	1.907531972931292E-4	3.2759788230776537E-4	0.0	0.021588285763218057	5.805532091530019E-5
36-37	1.907531972931292E-4	4.2297448095432997E-4	0.0	0.033361075483113574	5.805532091530019E-5
38-39	1.907531972931292E-4	4.312680982279443E-4	0.0	0.05239492712605842	5.805532091530019E-5
40-41	1.907531972931292E-4	4.8102980186963016E-4	0.0	0.09181863683618405	5.805532091530019E-5
42-43	1.907531972931292E-4	5.183510796008946E-4	0.0	0.10746039901422065	6.220212955210735E-5
44-45	1.9490000592993636E-4	5.639659746057733E-4	0.0	0.12973705501114868	6.63489381889145E-5
46-47	1.9904681456674352E-4	5.888468264266162E-4	0.0	0.15365170041961557	6.63489381889145E-5
48-49	2.0319362320355067E-4	5.888468264266162E-4	0.0	0.17296753504986329	6.63489381889145E-5
50-51	2.1563404911397214E-4	6.386085300683021E-4	0.0	0.1921631122296436	6.63489381889145E-5
52-53	2.1563404911397214E-4	6.427553387051093E-4	0.0	0.21870683431384624	6.63489381889145E-5
54-55	2.1563404911397214E-4	6.510489559787236E-4	0.0	0.27136715719266036	6.63489381889145E-5
56-57	2.1563404911397214E-4	6.551957646155307E-4	0.0	0.32876728234334496	6.63489381889145E-5
58-59	2.1563404911397214E-4	6.551957646155307E-4	0.0	0.38058994987752404	6.63489381889145E-5
60-61	2.488085182084294E-4	6.676361905259522E-4	0.0	0.4125328168068495	6.63489381889145E-5
62-63	2.9027660457650096E-4	6.800766164363737E-4	0.0	0.43100684928382543	6.63489381889145E-5
64-65	2.9027660457650096E-4	7.049574682572166E-4	0.0	0.4437541390333706	6.63489381889145E-5
66-67	2.985702218501153E-4	7.132510855308309E-4	0.0	0.45008631582177516	6.63489381889145E-5
68-69	3.068638391237296E-4	7.298383200780596E-4	0.0	0.4566092458074728	6.63489381889145E-5
70-71	3.1930426503415106E-4	7.381319373516739E-4	0.0	0.46197521618350124	6.63489381889145E-5
72-73	3.815063945862584E-4	7.381319373516739E-4	0.0	0.46532998437067824	7.464255546252882E-5
74-75	3.815063945862584E-4	7.42278745988481E-4	0.0	0.468195429138712	7.464255546252882E-5
76-77	3.815063945862584E-4	7.713064064461311E-4	0.0	0.47070010155534353	7.464255546252882E-5
78-79	3.815063945862584E-4	7.837468323565526E-4	0.0	0.4729974335401347	7.464255546252882E-5
80-81	3.815063945862584E-4	7.878936409933597E-4	0.0	0.4740424293166101	7.464255546252882E-5
82-83	3.8565320322306556E-4	7.920404496301669E-4	0.0	0.4744488165630172	7.464255546252882E-5
84-85	3.898000118598727E-4	8.169213014510098E-4	0.0	0.47473909316759366	7.464255546252882E-5
86-87	3.939468204966799E-4	8.459489619086599E-4	0.0	0.4748966718957924	7.464255546252882E-5
88-89	4.1468086368071566E-4	8.791234310031172E-4	0.0	0.4749837548771653	7.464255546252882E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12660	0.0	39.477943	1
GTATCAA	20295	0.0	31.86054	1
TCAACGC	21265	0.0	29.28573	4
ATCAACG	21815	0.0	28.567585	3
CAACGCA	22165	0.0	28.194405	5
AACGCAG	22940	0.0	27.27965	6
TATCAAC	25245	0.0	25.218794	2
GTACATG	28290	0.0	22.856476	1
ACGCAGA	27505	0.0	22.736214	7
CGCAGAG	28275	0.0	22.117048	8
TACATGG	28460	0.0	22.096022	2
CTTATAC	14125	0.0	21.677706	1
ACATGGG	29315	0.0	20.948568	3
TATACAC	18400	0.0	18.923706	3
CATGGGG	21915	0.0	18.556099	4
GCAGAGT	35380	0.0	17.663267	9
GAGTACT	19865	0.0	17.429659	12-13
TTATACA	18525	0.0	17.043222	2
AGAGTAC	29735	0.0	16.865652	10-11
CAGAGTA	32880	0.0	16.56299	10-11
>>END_MODULE