FastQCFastQC Report
Fri 27 May 2016
SRR2139763_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139763_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10108490
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA147050.051.9287381
GTATCAA239100.031.7085632
TCAACGC296850.025.638565
ATCAACG300800.025.3299354
CAACGCA304250.024.9460726
TATCAAC312550.024.6061363
AACGCAG314900.024.1557457
GTACATG335300.022.6612531
TACATGG332400.022.252422
GTGGTAT67350.022.0148661
ACATGGG338850.021.4319693
TGGTATC65700.021.161262
ACGCAGA365900.020.8118178
CTTATAC115300.020.8010731
CGCAGAG371200.020.480729
CATGGGG245850.019.7326934
TATACAC149400.018.5271843
GAGTACT252900.018.38509612-13
CAGAGTA417250.017.74705310-11
GCAGAGT432850.017.0493939
Produced by FastQC (version 0.11.5)