FastQCFastQC Report
Fri 27 May 2016
SRR2139762_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139762_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8561088
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80200.044.99711
GTATCAA134750.034.263251
CTTATAC64050.032.9005171
TCAACGC148350.030.2762954
ATCAACG152900.029.318733
CAACGCA153800.029.2315675
AACGCAG159150.028.2479276
TATCAAC166900.027.1705112
ACGCAGA188900.023.8219057
TATACAC102050.023.7447683
CGCAGAG192950.023.2546128
TTATACA101200.022.2339022
GTACATG192600.021.7251641
TACATGG195950.020.8900152
ACATGGG201000.019.6762813
ACATCTC110300.019.5749288
ACACATC110200.019.3964826
TATGCCG60000.018.56621442-43
GCAGAGT245300.018.3623529
CATGGGG145700.018.2942264
Produced by FastQC (version 0.11.5)