##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139762_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8561088
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09879164891191	33.0	33.0	34.0	30.0	34.0
2	32.45141855801506	33.0	33.0	34.0	31.0	34.0
3	32.80827682182451	33.0	33.0	34.0	32.0	34.0
4	32.99732358784304	33.0	33.0	34.0	32.0	34.0
5	33.12020107724626	34.0	33.0	34.0	32.0	34.0
6	36.7602229996935	38.0	37.0	38.0	34.0	38.0
7	37.18306843709585	38.0	38.0	38.0	36.0	38.0
8	37.34445318165168	38.0	38.0	38.0	37.0	38.0
9	37.344370715497845	38.0	38.0	38.0	37.0	38.0
10-11	37.392340611380234	38.0	38.0	38.0	37.0	38.0
12-13	37.39722824949352	38.0	38.0	38.0	37.0	38.0
14-15	37.4097153889786	38.0	38.0	38.0	37.0	38.0
16-17	37.36411440929004	38.0	38.0	38.0	37.0	38.0
18-19	37.348304678096994	38.0	38.0	38.0	37.0	38.0
20-21	37.40763304218727	38.0	38.0	38.0	37.0	38.0
22-23	37.423564712795	38.0	38.0	38.0	37.0	38.0
24-25	37.42418013419824	38.0	38.0	38.0	37.0	38.0
26-27	37.333230907313286	38.0	38.0	38.0	37.0	38.0
28-29	37.24521300595846	38.0	38.0	38.0	37.0	38.0
30-31	37.32560428799627	38.0	38.0	38.0	37.0	38.0
32-33	37.34309995029951	38.0	38.0	38.0	37.0	38.0
34-35	37.2956027252264	38.0	38.0	38.0	37.0	38.0
36-37	37.303245524480104	38.0	38.0	38.0	37.0	38.0
38-39	37.28871035228102	38.0	38.0	38.0	37.0	38.0
40-41	37.26914723391293	38.0	38.0	38.0	37.0	38.0
42-43	37.274749736739274	38.0	38.0	38.0	37.0	38.0
44-45	37.21601971351168	38.0	38.0	38.0	37.0	38.0
46-47	37.19991311349119	38.0	38.0	38.0	37.0	38.0
48-49	37.18333191964268	38.0	38.0	38.0	37.0	38.0
50-51	37.18322643679484	38.0	38.0	38.0	37.0	38.0
52-53	37.16782028365491	38.0	38.0	38.0	36.5	38.0
54-55	37.12722364401162	38.0	38.0	38.0	36.0	38.0
56-57	37.120336127091974	38.0	38.0	38.0	36.0	38.0
58-59	37.130568711580466	38.0	38.0	38.0	36.0	38.0
60-61	37.13874419038105	38.0	38.0	38.0	36.0	38.0
62-63	37.07432805751159	38.0	38.0	38.0	36.0	38.0
64-65	37.100991320288955	38.0	38.0	38.0	36.0	38.0
66-67	37.117063983417374	38.0	38.0	38.0	36.0	38.0
68-69	37.08543833098375	38.0	38.0	38.0	36.0	38.0
70-71	37.03592437017902	38.0	38.0	38.0	36.0	38.0
72-73	37.05311648561674	38.0	38.0	38.0	36.0	38.0
74-75	37.07269757724926	38.0	38.0	38.0	36.0	38.0
76-77	37.01211278261947	38.0	38.0	38.0	36.0	38.0
78-79	37.03841406645619	38.0	38.0	38.0	36.0	38.0
80-81	37.01670465467389	38.0	38.0	38.0	36.0	38.0
82-83	36.97623792835826	38.0	38.0	38.0	36.0	38.0
84-85	36.999983378131446	38.0	38.0	38.0	36.0	38.0
86-87	37.02320953880668	38.0	38.0	38.0	36.0	38.0
88-89	36.93491586835468	38.0	38.0	38.0	36.0	38.0
90-91	36.89720432147526	38.0	38.0	38.0	35.0	38.0
92-93	36.95195116213662	38.0	38.0	38.0	36.0	38.0
94-95	36.937373009000005	38.0	38.0	38.0	35.5	38.0
96-97	36.88780805589764	38.0	38.0	38.0	35.0	38.0
98-99	36.90073536691679	38.0	38.0	38.0	35.0	38.0
100	35.36826013288148	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	8.0
19	20.0
20	70.0
21	214.0
22	527.0
23	1429.0
24	3502.0
25	7054.0
26	12495.0
27	20222.0
28	31697.0
29	45535.0
30	62540.0
31	84730.0
32	111189.0
33	149730.0
34	218313.0
35	383807.0
36	1056520.0
37	6371485.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.54035094604798	20.28526047156623	12.917715598765017	25.256672983620774
2	14.428558462913019	21.757718100187628	40.52703882934355	23.286684607555802
3	18.49094414167919	25.76048745206217	30.388252053944548	25.3603163523141
4	12.443749828438802	17.75767907922886	38.97841219892105	30.820158893411293
5	13.106119799499744	37.94864888006208	35.86634032595368	13.078890994484501
6	30.20928483155123	38.121973213597386	18.611562119644063	13.057179835207322
7	26.48749924075	32.45402449223696	23.208111593397096	17.850364673615946
8	24.36988149169825	36.829407664072605	21.505946440452426	17.294764403776718
9	25.409644194756552	16.486374161788785	21.146751440938345	36.957230202516314
10-11	23.963899214679255	26.788557716028617	29.38854267121188	19.859000398080244
12-13	24.705457997861956	24.169982833957555	29.389331122399398	21.735228045781096
14-15	21.74522677491459	25.72682934692413	26.6396163665179	25.88832751164338
16-17	21.728219590780984	28.94978418630903	27.368892832312902	21.953103390597086
18-19	21.74942600753549	28.18441417726345	28.858434815761736	21.20772499943932
20-21	22.26859234915294	27.439655482059667	28.77780759664745	21.51394457213994
22-23	21.95726887450437	27.5199886550916	28.658100707906165	21.86464176249787
24-25	21.84247190603025	27.595070444541626	28.817482958576708	21.74497469085141
26-27	21.959317665258148	27.736332103486145	28.661682047983263	21.642668183272445
28-29	21.789605619592255	27.60838161140145	28.813873884076425	21.788138884929875
30-31	22.1223902069303	27.62767228110367	28.649849581226345	21.600087930739683
32-33	21.841196513532914	27.619483209471095	28.72688813671212	21.81243214028387
34-35	21.84345694094631	27.794538395432884	28.83928182166768	21.522722841953126
36-37	21.960957804434443	27.67728284323966	28.639831546604537	21.72192780572136
38-39	21.69370768469058	27.689032671188997	28.8705478359842	21.746711808136222
40-41	21.973784215263574	27.520341236662016	28.792254897335496	21.713619650738913
42-43	21.918922000485942	27.59560791465913	28.669350197678146	21.81611988717678
44-45	21.85237309799791	27.7662621839051	28.585153405155307	21.796211312941686
46-47	22.03831329934144	27.602222854329128	28.36136730155306	21.998096544776377
48-49	21.91876376267584	27.682653404397477	28.388842167322903	22.00974066560378
50-51	21.822445755330737	27.817084496255013	28.457617440186873	21.90285230822738
52-53	21.965474927024	27.769082204629186	28.51389724418249	21.751545624164322
54-55	21.918409245599797	27.733795286889574	28.374750225183	21.973045242327633
56-57	22.063750290932607	27.857278918418064	28.20964303316006	21.86932775748927
58-59	21.833762964378845	28.05998988291248	28.405531426084362	21.700715726624313
60-61	22.035174812088343	28.202215815947284	28.13763643152372	21.62497294044065
62-63	21.869372350695627	28.355449419067792	28.090174567076232	21.685003663160348
64-65	21.83326206929426	28.312647574285727	28.12412503207205	21.729965324347962
66-67	21.888500035005976	28.280102303497507	28.032087898505875	21.79930976299064
68-69	21.91764275785706	28.200921582628798	28.06816134534231	21.81327431417183
70-71	21.960540298859936	27.93281024662645	28.160232231392897	21.946417223120715
72-73	21.99517182732712	27.805516350039706	28.19820558408002	22.00110623855315
74-75	21.879741680147816	27.915996657646847	28.174081703045523	22.030179959159813
76-77	22.024584774879518	27.845846008607406	28.11382133274457	22.01574788376851
78-79	21.973891716734375	27.85072172858621	28.1067829470641	22.068603607615316
80-81	22.013384580695085	27.914809951177805	28.025140719035335	22.04666474909177
82-83	22.05006898666898	27.888155906739463	28.110008903773604	21.95176620281795
84-85	22.10283141924169	27.838857203615277	28.095661518516913	21.962649858626122
86-87	21.960408042375665	27.847174141815835	28.163431222900996	22.028986592907504
88-89	22.04730848999189	27.86862601265246	28.06542355211687	22.01864194523878
90-91	22.08538397326423	27.907011329597776	28.057725865791355	21.94987883134664
92-93	22.10544864823192	27.88186410253326	28.099155888542914	21.913531360691902
94-95	22.04438512723899	27.950114191224745	28.04868055645406	21.956820125082206
96-97	22.136484689549928	28.02761696280081	28.13347704821486	21.702421299434402
98-99	23.13684406477838	29.9578136203063	28.387007987217643	18.51833432769768
100	22.04425692482898	30.865223603850268	22.394807490772305	24.69571198054845
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	961.0
1	940.5
2	1446.5
3	2196.0
4	2752.5
5	3652.5
6	4678.5
7	6043.0
8	7688.5
9	8695.5
10	8759.0
11	8812.5
12	9726.0
13	11721.0
14	15242.0
15	18985.0
16	21391.5
17	22353.0
18	22128.5
19	21439.0
20	20937.5
21	21562.5
22	24423.5
23	29731.0
24	38554.0
25	50748.0
26	65869.0
27	83657.0
28	102496.5
29	123575.0
30	145530.0
31	167260.5
32	189367.5
33	209611.0
34	228896.5
35	247069.5
36	265924.5
37	284068.5
38	298027.5
39	310900.5
40	325269.5
41	337156.5
42	345156.0
43	361742.5
44	372865.0
45	372594.0
46	369148.0
47	361631.5
48	355787.0
49	347140.5
50	337862.0
51	326974.0
52	310314.0
53	290955.5
54	271751.0
55	250960.5
56	228614.0
57	205739.0
58	182401.5
59	158301.0
60	132559.5
61	107153.0
62	84328.5
63	65368.5
64	49457.5
65	36610.0
66	27047.5
67	20424.0
68	15350.5
69	11046.0
70	8011.0
71	5985.5
72	4417.5
73	3064.0
74	2014.5
75	1386.0
76	924.0
77	563.5
78	350.5
79	213.5
80	135.5
81	88.0
82	59.0
83	43.5
84	29.0
85	18.5
86	15.0
87	11.5
88	7.0
89	4.0
90	3.0
91	2.5
92	2.0
93	2.0
94	1.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	1.2848834166872248E-4
3	0.0
4	3.504227500056068E-5
5	0.003515908258389588
6	3.270612333385663E-4
7	9.344606666816181E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	9.700222795418385E-4
30-31	0.0
32-33	0.0
34-35	5.875649516331221E-6
36-37	0.0
38-39	0.0
40-41	0.0
42-43	1.79933654862779E-5
44-45	1.8108912796409703E-5
46-47	3.0402738386807594E-5
48-49	8.574630058304422E-5
50-51	4.6249433059033086E-4
52-53	1.675537811954888E-4
54-55	3.185216001051246E-4
56-57	3.83611367725455E-4
58-59	3.546731512741158E-4
60-61	3.189104386593949E-5
62-63	2.954411819959428E-4
64-65	5.174212835682717E-5
66-67	7.170678911404893E-5
68-69	4.2081221491616235E-4
70-71	6.633661987060379E-5
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0011326861302512112
90-91	0.0
92-93	2.173714416385575E-5
94-95	8.771843260318703E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	104.0
20-21	246.0
22-23	403.0
24-25	579.0
26-27	2019.0
28-29	6441.0
30-31	15455.0
32-33	20038.0
34-35	27885.0
36-37	44002.0
38-39	49249.0
40-41	46433.0
42-43	50712.0
44-45	59196.0
46-47	60285.0
48-49	56580.0
50-51	52030.0
52-53	50495.0
54-55	54061.0
56-57	56375.0
58-59	55535.0
60-61	54915.0
62-63	53748.0
64-65	57966.0
66-67	64429.0
68-69	66974.0
70-71	75421.0
72-73	55915.0
74-75	54205.0
76-77	56399.0
78-79	58385.0
80-81	57914.0
82-83	54425.0
84-85	53514.0
86-87	55690.0
88-89	58516.0
90-91	59308.0
92-93	58984.0
94-95	71419.0
96-97	255464.0
98-99	585581.0
100-101	5943793.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.39466337708367
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.51691606397296	78.20178493278681
2	6.746855222568591	11.657845716154648
3	1.554140769823767	4.0280840594857805
4	0.5715321767610871	1.9750932008178406
5	0.24366770610918057	1.0525794722584405
6	0.12168580564504758	0.6307802529883869
7	0.06882851027600916	0.41624911832293693
8	0.044692043856683177	0.30889232677056033
9	0.03102078331354594	0.24120271188605302
>10	0.09842577772249587	1.2811198440966185
>50	0.001818658530684181	0.10213561492866557
>100	3.542771363333794E-4	0.05866024743082333
>500	4.1469568111533523E-5	0.02538377607117462
>1k	2.0734715510460293E-5	0.020188726001225312
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.1680758333520226E-5	0.0	0.0	0.0
3	0.0	1.1680758333520226E-5	0.0	0.0	0.0
4	0.0	2.3361516667040452E-5	0.0	0.0	0.0
5	0.0	2.3361516667040452E-5	0.0	0.0	0.0
6	2.3361516667040452E-5	2.3361516667040452E-5	0.0	0.0	0.0
7	2.3361516667040452E-5	2.3361516667040452E-5	0.0	0.0	0.0
8	2.3361516667040452E-5	3.5042275000560675E-5	0.0	0.0	0.0
9	2.3361516667040452E-5	7.008455000112135E-5	0.0	0.0	1.1680758333520226E-5
10-11	2.3361516667040452E-5	7.008455000112135E-5	0.0	0.0	1.1680758333520226E-5
12-13	2.3361516667040452E-5	7.008455000112135E-5	0.0	5.840379166760113E-6	1.1680758333520226E-5
14-15	2.3361516667040452E-5	8.176530833464158E-5	0.0	1.1680758333520226E-5	2.9201895833800563E-5
16-17	4.6723033334080904E-5	8.176530833464158E-5	0.0	4.6723033334080904E-5	3.5042275000560675E-5
18-19	4.6723033334080904E-5	1.0512682500168202E-4	0.0	9.344606666816181E-5	4.088265416732079E-5
20-21	4.6723033334080904E-5	1.2848834166872248E-4	0.0	9.344606666816181E-5	4.6723033334080904E-5
22-23	4.6723033334080904E-5	1.401691000022427E-4	0.0	9.344606666816181E-5	6.424417083436124E-5
24-25	4.6723033334080904E-5	1.6353061666928316E-4	0.0	1.1680758333520225E-4	8.176530833464158E-5
26-27	4.6723033334080904E-5	1.7521137500280337E-4	0.0	1.2848834166872248E-4	8.176530833464158E-5
28-29	4.6723033334080904E-5	1.7521137500280337E-4	0.0	2.861785791712455E-4	9.344606666816181E-5
30-31	4.6723033334080904E-5	1.9857289166984383E-4	0.0	8.234934625131759E-4	9.344606666816181E-5
32-33	4.6723033334080904E-5	1.9857289166984383E-4	0.0	0.0021083768792004008	9.344606666816181E-5
34-35	4.6723033334080904E-5	2.628170625042051E-4	0.0	0.003492546741722547	9.344606666816181E-5
36-37	4.6723033334080904E-5	3.036997166715259E-4	0.0	0.005822858029259833	9.344606666816181E-5
38-39	4.6723033334080904E-5	3.1538047500504607E-4	0.0	0.009420531595984062	9.344606666816181E-5
40-41	4.6723033334080904E-5	3.854650250061674E-4	0.0	0.013117491608543214	9.344606666816181E-5
42-43	4.6723033334080904E-5	3.9130540417292754E-4	0.0	0.016481550008597037	1.0512682500168202E-4
44-45	4.6723033334080904E-5	4.205073000067281E-4	0.0	0.02025443495032407	1.1680758333520225E-4
46-47	4.6723033334080904E-5	4.5554957500728877E-4	0.0	0.02479824994206344	1.1680758333520225E-4
48-49	5.256341250084101E-5	4.5554957500728877E-4	0.0	0.029125970904632682	1.401691000022427E-4
50-51	6.424417083436124E-5	5.022726083413697E-4	0.0	0.033570499450537125	1.401691000022427E-4
52-53	7.008455000112135E-5	5.022726083413697E-4	0.0	0.037734689796437085	1.401691000022427E-4
54-55	7.008455000112135E-5	5.022726083413697E-4	0.0	0.04240115275067842	1.4600947916900282E-4
56-57	7.008455000112135E-5	5.139533666748899E-4	0.0	0.046512779684077535	1.6937099583604328E-4
58-59	7.008455000112135E-5	5.139533666748899E-4	0.0	0.0508813833008141	1.9273251250308374E-4
60-61	9.344606666816181E-5	5.139533666748899E-4	0.0	0.05520910426338335	1.9857289166984383E-4
62-63	1.1680758333520225E-4	5.139533666748899E-4	0.0	0.05881845858844109	1.9857289166984383E-4
64-65	1.1680758333520225E-4	5.957186750095315E-4	0.0	0.062427812913498845	1.9857289166984383E-4
66-67	1.3432872083548258E-4	5.957186750095315E-4	0.0	0.06625910164689347	1.9857289166984383E-4
68-69	1.401691000022427E-4	6.015590541762917E-4	0.0	0.07012543265528867	2.1025365000336405E-4
70-71	1.401691000022427E-4	6.073994333430518E-4	0.0	0.07360045825951095	2.219344083368843E-4
72-73	1.401691000022427E-4	6.073994333430518E-4	0.0	0.07667833808039352	2.452959250039247E-4
74-75	1.401691000022427E-4	6.424417083436124E-4	0.0	0.07904953202209812	2.452959250039247E-4
76-77	1.401691000022427E-4	6.716436041774129E-4	0.0	0.08155505468463822	2.452959250039247E-4
78-79	1.401691000022427E-4	6.774839833441731E-4	0.0	0.08309691478466288	2.452959250039247E-4
80-81	1.401691000022427E-4	6.774839833441731E-4	0.0	0.08358750663467074	2.5697668333744497E-4
82-83	1.401691000022427E-4	6.833243625109332E-4	0.0	0.08394961014300986	3.036997166715259E-4
84-85	1.401691000022427E-4	6.950051208444534E-4	0.0	0.08420074644718054	3.036997166715259E-4
86-87	1.401691000022427E-4	7.008455000112135E-4	0.0	0.0843233944096825	3.036997166715259E-4
88	1.401691000022427E-4	7.125262583447337E-4	0.0	0.0843233944096825	3.036997166715259E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8020	0.0	44.9971	1
GTATCAA	13475	0.0	34.26325	1
CTTATAC	6405	0.0	32.900517	1
TCAACGC	14835	0.0	30.276295	4
ATCAACG	15290	0.0	29.31873	3
CAACGCA	15380	0.0	29.231567	5
AACGCAG	15915	0.0	28.247927	6
TATCAAC	16690	0.0	27.170511	2
ACGCAGA	18890	0.0	23.821905	7
TATACAC	10205	0.0	23.744768	3
CGCAGAG	19295	0.0	23.254612	8
TTATACA	10120	0.0	22.233902	2
GTACATG	19260	0.0	21.725164	1
TACATGG	19595	0.0	20.890015	2
ACATGGG	20100	0.0	19.676281	3
ACATCTC	11030	0.0	19.574928	8
ACACATC	11020	0.0	19.396482	6
TATGCCG	6000	0.0	18.566214	42-43
GCAGAGT	24530	0.0	18.362352	9
CATGGGG	14570	0.0	18.294226	4
>>END_MODULE