FastQCFastQC Report
Fri 27 May 2016
SRR2139761_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139761_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5636335
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64250.049.386061
GTATCAA113150.035.8182681
TCAACGC137750.028.846574
ATCAACG139200.028.516133
CAACGCA141800.028.022675
TATCAAC147900.027.261592
AACGCAG146250.027.1975616
ACGCAGA171850.023.0732337
CGCAGAG174650.022.7271948
GTACATG152450.021.33341
TACATGG152850.021.1138972
ACATGGG155100.020.296823
GCAGAGT210100.018.8329189
CATGGGG114350.018.7057044
GAGTACT128050.017.82715412-13
AGAGTAC187250.017.62408410-11
CAGAGTA207150.016.96758810-11
TATACCG3756.184564E-1116.6783435
GTGGTAT31800.015.7342841
TGGTATC29550.015.5212992
Produced by FastQC (version 0.11.5)