##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139761_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5636335
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.21115671797365	33.0	33.0	34.0	30.0	34.0
2	32.516315478054445	33.0	33.0	34.0	31.0	34.0
3	32.827699737506734	33.0	33.0	34.0	32.0	34.0
4	32.999439351990254	33.0	33.0	34.0	32.0	34.0
5	33.12132263252628	34.0	33.0	34.0	32.0	34.0
6	36.74673826165407	38.0	37.0	38.0	34.0	38.0
7	37.20723324642698	38.0	38.0	38.0	36.0	38.0
8	37.374570709512476	38.0	38.0	38.0	37.0	38.0
9	37.381336808404754	38.0	38.0	38.0	37.0	38.0
10-11	37.426328545056315	38.0	38.0	38.0	37.0	38.0
12-13	37.42945921418794	38.0	38.0	38.0	37.0	38.0
14-15	37.43992789640786	38.0	38.0	38.0	37.0	38.0
16-17	37.39817230523026	38.0	38.0	38.0	37.0	38.0
18-19	37.381830391557635	38.0	38.0	38.0	37.0	38.0
20-21	37.441919760374205	38.0	38.0	38.0	37.0	38.0
22-23	37.45784570382445	38.0	38.0	38.0	37.0	38.0
24-25	37.45895265813982	38.0	38.0	38.0	37.0	38.0
26-27	37.36892732747326	38.0	38.0	38.0	37.0	38.0
28-29	37.2825581487013	38.0	38.0	38.0	37.0	38.0
30-31	37.361284177684865	38.0	38.0	38.0	37.0	38.0
32-33	37.37920604730382	38.0	38.0	38.0	37.0	38.0
34-35	37.33342762035528	38.0	38.0	38.0	37.0	38.0
36-37	37.33830923111128	38.0	38.0	38.0	37.0	38.0
38-39	37.3327906504683	38.0	38.0	38.0	37.0	38.0
40-41	37.29599088342037	38.0	38.0	38.0	37.0	38.0
42-43	37.296973071105775	38.0	38.0	38.0	37.0	38.0
44-45	37.23512447414045	38.0	38.0	38.0	37.0	38.0
46-47	37.21581142504255	38.0	38.0	38.0	37.0	38.0
48-49	37.198159361459105	38.0	38.0	38.0	37.0	38.0
50-51	37.20080650759688	38.0	38.0	38.0	37.0	38.0
52-53	37.18490059923677	38.0	38.0	38.0	37.0	38.0
54-55	37.14600229952197	38.0	38.0	38.0	36.5	38.0
56-57	37.1420132656027	38.0	38.0	38.0	36.0	38.0
58-59	37.155271921065726	38.0	38.0	38.0	36.0	38.0
60-61	37.16873602349082	38.0	38.0	38.0	36.0	38.0
62-63	37.103141300655395	38.0	38.0	38.0	36.0	38.0
64-65	37.13655498562846	38.0	38.0	38.0	36.0	38.0
66-67	37.15549989515224	38.0	38.0	38.0	36.0	38.0
68-69	37.129679753383314	38.0	38.0	38.0	36.0	38.0
70-71	37.08403196464242	38.0	38.0	38.0	36.0	38.0
72-73	37.1060174692695	38.0	38.0	38.0	36.0	38.0
74-75	37.130248136854455	38.0	38.0	38.0	36.0	38.0
76-77	37.0705229086053	38.0	38.0	38.0	36.0	38.0
78-79	37.09788008082394	38.0	38.0	38.0	36.0	38.0
80-81	37.07884393417563	38.0	38.0	38.0	36.0	38.0
82-83	37.03570026369089	38.0	38.0	38.0	36.0	38.0
84-85	37.063688595367594	38.0	38.0	38.0	36.0	38.0
86-87	37.089008640772164	38.0	38.0	38.0	36.0	38.0
88-89	37.00497937081221	38.0	38.0	38.0	36.0	38.0
90-91	36.967407773625496	38.0	38.0	38.0	36.0	38.0
92-93	37.02536913643583	38.0	38.0	38.0	36.0	38.0
94-95	37.012132131963455	38.0	38.0	38.0	36.0	38.0
96-97	36.96064587432568	38.0	38.0	38.0	36.0	38.0
98-99	36.97244435409955	38.0	38.0	38.0	35.5	38.0
100	35.45102407757845	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	4.0
19	12.0
20	42.0
21	85.0
22	285.0
23	788.0
24	2004.0
25	4015.0
26	7363.0
27	12561.0
28	19548.0
29	28435.0
30	39508.0
31	52967.0
32	69702.0
33	92714.0
34	134494.0
35	239427.0
36	677398.0
37	4254983.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.847086803747466	20.58768685679613	12.550975057373275	25.014251282083126
2	14.45869918901129	22.72943564340626	40.21501579928783	22.596849368294617
3	17.799545271883236	27.424363527008243	31.011996270626213	23.764094930482308
4	12.329862754713526	18.374077325466278	39.81812994692292	29.477929972897265
5	12.667659547589796	38.473053467240966	36.094202327698966	12.765084657470277
6	28.717369689890077	38.86180070556625	19.497016140307053	12.92381346423662
7	25.22395770866585	33.14073762865637	24.65610613427314	16.979198528404645
8	24.140456520061353	36.294436012053936	22.476236774428774	17.08887069345594
9	25.84676035047597	16.112331861040907	21.645998685315902	36.394909103167215
10-11	23.709884171185706	26.632953860975263	30.0025725937156	19.654589374123432
12-13	24.6525623476958	24.61798313975305	29.787876341629797	20.941578170921353
14-15	21.890794283874186	25.65750616313615	27.276164386964226	25.17553516602544
16-17	21.48757126749918	28.63963905623069	27.98298007481812	21.889809601452008
18-19	21.56484666010803	27.97171388854637	29.510896708588113	20.952542742757483
20-21	22.249206448760955	27.31312834172653	29.266540512253393	21.171124697259128
22-23	21.96779825832135	27.290776019408803	29.176386548650335	21.565039173619517
24-25	21.782432878203842	27.34215839869088	29.253546111560937	21.621862611544344
26-27	21.726327700701336	27.494699774574038	29.20076363000043	21.57820889472419
28-29	21.718432011938347	27.450898048671434	29.152759278492663	21.677910660897552
30-31	21.875877470097777	27.410143060857546	29.128655188105583	21.585324280939094
32-33	21.596234965027982	27.52913918750661	29.213309247779335	21.661316599686074
34-35	21.77141882738631	27.53221956271898	29.1523097329409	21.54405187695381
36-37	21.795107162861115	27.4808748524426	29.134965238038298	21.589052746657988
38-39	21.64969755251977	27.59258038030044	29.163327074466505	21.594394992713287
40-41	21.854437271116613	27.446334581150655	29.045033715371744	21.65419443236098
42-43	21.80744236312965	27.466105631009434	28.94090609874795	21.78554590711297
44-45	21.786527455398915	27.65744590039297	28.808313257705244	21.747713386502877
46-47	21.90720037093099	27.62257922158975	28.592158709767357	21.87806169771191
48-49	21.831889710561608	27.708482365931413	28.545618403646344	21.914009519860635
50-51	21.808864510139248	27.798169969351115	28.46211559509092	21.930849925418716
52-53	21.98911249105356	27.69785298075609	28.41268658935575	21.9003479388346
54-55	21.932257152067418	27.69332363549327	28.38064289483459	21.99377631760472
56-57	21.928109457476985	27.84264287950824	28.30398201605825	21.925265646956525
58-59	21.918800007981325	27.90066244986731	28.31468364028174	21.865853901869624
60-61	22.027427455472516	27.90507648057924	28.254664237716042	21.812831826232205
62-63	21.919484300914714	27.960516457490282	28.24095301334487	21.879046228250136
64-65	21.915563234273545	27.944593772556047	28.225484636116267	21.91435835705414
66-67	21.967229397445408	27.962249225368456	28.134505250399037	21.9360161267871
68-69	21.94912397502128	27.885225558523864	28.185966745603018	21.97968372085184
70-71	22.027100755379543	27.662192460537522	28.225879726241036	22.084827057841906
72-73	22.072832628495757	27.63236884281332	28.181998526358328	22.112800002332598
74-75	21.888289604160143	27.799707548858244	28.185631238350737	22.12637160863088
76-77	22.0701776313741	27.721626270337378	28.094824622169796	22.11337147611873
78-79	22.018260585911666	27.734686399628224	28.076125618889307	22.170927395570807
80-81	22.098067816184916	27.74752375851407	27.984908119040576	22.16950030626044
82-83	22.072293926976062	27.747259388285684	28.065729084000363	22.114717600737897
84-85	22.13220181307888	27.694355507407952	28.020059063261133	22.153383616252043
86-87	21.996720484211274	27.77705767680359	28.05258720810544	22.173634630879693
88-89	22.117342916146583	27.713499721672854	28.003047824773585	22.166109537406978
90-91	22.175723660926845	27.771586524014406	27.951477732778912	22.10121208227984
92-93	22.19165618795762	27.72364641761052	27.939166628360905	22.145530766070955
94-95	22.09881149886803	27.766139700515858	27.990160156395323	22.144888644220785
96-97	22.18852998677441	27.885686295573514	28.014747152673458	21.91103656497862
98-99	23.222664172867134	29.75588806215986	28.27935999738641	18.742087767586597
100	29.505293170540504	40.82376476706751	29.670942062391987	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1402.0
1	1367.5
2	1867.5
3	2654.5
4	3420.5
5	4624.0
6	5713.5
7	6831.5
8	7917.5
9	8296.5
10	7986.5
11	7749.5
12	8442.5
13	10515.5
14	14413.5
15	18465.0
16	20932.5
17	21984.5
18	21677.0
19	20298.0
20	19047.0
21	18736.0
22	20228.5
23	24393.5
24	31435.0
25	40891.5
26	52940.5
27	66561.5
28	80975.5
29	96525.0
30	110683.0
31	124016.5
32	137834.0
33	150709.5
34	162534.0
35	173826.5
36	186090.0
37	197311.5
38	206611.5
39	214209.0
40	221056.0
41	228383.5
42	232935.5
43	236562.5
44	240850.5
45	243333.0
46	242589.0
47	240076.5
48	236056.5
49	229508.0
50	222960.5
51	215406.5
52	205287.5
53	193529.0
54	180243.5
55	167458.5
56	154463.0
57	139994.0
58	124831.5
59	108301.0
60	91042.0
61	73517.5
62	57331.0
63	44204.5
64	33863.0
65	25500.0
66	18810.0
67	14418.0
68	11091.5
69	8149.0
70	5964.5
71	4404.0
72	3246.5
73	2242.5
74	1423.0
75	937.0
76	649.5
77	403.0
78	246.5
79	163.5
80	104.5
81	62.0
82	41.0
83	31.0
84	24.5
85	21.5
86	18.5
87	11.0
88	4.0
89	0.5
90	0.5
91	0.5
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	8.871012812403804E-5
3	0.0
4	5.322607687442283E-5
5	0.003743567406834406
6	1.9516228187288373E-4
7	5.322607687442283E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0011186750909411823
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	1.8787019822560356E-5
48-49	9.492513159945619E-5
50-51	5.372087573085573E-4
52-53	1.8403790793455226E-4
54-55	3.715383987379427E-4
56-57	5.035889103007433E-4
58-59	4.888536869893786E-4
60-61	3.0255977673508958E-5
62-63	4.892911496405239E-4
64-65	1.235769979954781E-4
66-67	1.874042091193595E-4
68-69	7.057871491329299E-4
70-71	8.531498719848617E-5
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	9.736605814463514E-4
90-91	0.0
92-93	0.0
94-95	4.94389727174737E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	87.0
20-21	189.0
22-23	412.0
24-25	650.0
26-27	2134.0
28-29	6160.0
30-31	14040.0
32-33	18661.0
34-35	23837.0
36-37	38597.0
38-39	47753.0
40-41	44754.0
42-43	47535.0
44-45	54493.0
46-47	55828.0
48-49	55730.0
50-51	51752.0
52-53	47601.0
54-55	49575.0
56-57	51678.0
58-59	53411.0
60-61	53941.0
62-63	49715.0
64-65	51643.0
66-67	55393.0
68-69	57176.0
70-71	60339.0
72-73	52076.0
74-75	50034.0
76-77	51824.0
78-79	54788.0
80-81	56762.0
82-83	52476.0
84-85	50277.0
86-87	50835.0
88-89	53717.0
90-91	56033.0
92-93	54842.0
94-95	59897.0
96-97	166482.0
98-99	1225886.0
100-101	2607322.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.5715481679162
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.5860262905725	82.93073713561971
2	5.539091623726092	9.922900243621656
3	1.1096772300366362	2.981865223831991
4	0.36776507273836023	1.3176514770904497
5	0.15661343458559346	0.7014053899863139
6	0.0829656735546977	0.445881829505293
7	0.04435038836716518	0.27807730635267286
8	0.025985007333422867	0.18620138688074736
9	0.020795672454747376	0.16764305192681514
>10	0.06519398028041418	0.872968260953052
>50	7.473878440910638E-4	0.04505013358165045
>100	7.334369945109159E-4	0.11857131013044868
>500	5.480151173839331E-5	0.03104725051926007
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.774202562480761E-5
2	0.0	1.774202562480761E-5	0.0	0.0	1.774202562480761E-5
3	0.0	1.774202562480761E-5	0.0	0.0	1.774202562480761E-5
4	0.0	1.596782306232685E-4	0.0	0.0	1.774202562480761E-5
5	0.0	1.596782306232685E-4	0.0	1.774202562480761E-5	1.774202562480761E-5
6	7.096810249923045E-5	1.596782306232685E-4	0.0	1.774202562480761E-5	1.774202562480761E-5
7	7.096810249923045E-5	1.596782306232685E-4	0.0	3.548405124961522E-5	1.774202562480761E-5
8	7.096810249923045E-5	1.951622818728837E-4	0.0	3.548405124961522E-5	1.774202562480761E-5
9	7.096810249923045E-5	2.6613038437211416E-4	0.0	5.322607687442283E-5	1.774202562480761E-5
10-11	7.096810249923045E-5	2.6613038437211416E-4	0.0	5.322607687442283E-5	1.774202562480761E-5
12-13	7.096810249923045E-5	2.6613038437211416E-4	0.0	5.322607687442283E-5	1.774202562480761E-5
14-15	7.096810249923045E-5	3.459694996837484E-4	0.0	5.322607687442283E-5	3.548405124961522E-5
16-17	8.871012812403805E-5	3.548405124961522E-4	0.0	7.096810249923045E-5	3.548405124961522E-5
18-19	8.871012812403805E-5	3.9919557655817124E-4	0.0	7.096810249923045E-5	3.548405124961522E-5
20-21	1.0645215374884566E-4	4.5242165343259405E-4	0.0	8.871012812403805E-5	6.209708968682664E-5
22-23	1.0645215374884566E-4	5.145187431194206E-4	0.0	1.0645215374884566E-4	8.871012812403805E-5
24-25	1.0645215374884566E-4	5.50002794369036E-4	0.0	1.2419417937365327E-4	1.0645215374884566E-4
26-27	1.0645215374884566E-4	5.677448199938436E-4	0.0	1.508072178108647E-4	1.0645215374884566E-4
28-29	1.0645215374884566E-4	5.677448199938436E-4	0.0	4.967767174946131E-4	1.0645215374884566E-4
30-31	1.0645215374884566E-4	6.564549481178816E-4	0.0	0.0015258142037334545	1.0645215374884566E-4
32-33	1.419362049984609E-4	7.096810249923044E-4	0.0	0.003725825381209598	1.0645215374884566E-4
34-35	1.419362049984609E-4	7.895201403039386E-4	0.0	0.006449226314617566	1.0645215374884566E-4
36-37	1.419362049984609E-4	8.693592556155729E-4	0.0	0.010192793721451971	1.0645215374884566E-4
38-39	1.419362049984609E-4	8.871012812403805E-4	0.0	0.015506530396081852	1.1532316656124946E-4
40-41	1.419362049984609E-4	0.0010645215374884567	0.0	0.021689626326327302	1.2419417937365327E-4
42-43	1.596782306232685E-4	0.0011088766015504756	0.0	0.027961432384696793	1.2419417937365327E-4
44-45	1.596782306232685E-4	0.0012064577424869176	0.0	0.033975979071506575	1.2419417937365327E-4
46-47	1.596782306232685E-4	0.001277425844986148	0.0	0.040496173488623374	1.419362049984609E-4
48-49	1.596782306232685E-4	0.0013129098962357632	0.0	0.04757524171292161	1.596782306232685E-4
50-51	1.774202562480761E-4	0.0014903301524838391	0.0	0.05511560260346484	1.774202562480761E-4
52-53	1.774202562480761E-4	0.001499201165296243	0.0	0.06192854044339097	1.9516228187288373E-4
54-55	1.774202562480761E-4	0.0015169431909210507	0.0	0.06837776675800852	2.1290430749769132E-4
56-57	1.862912690604799E-4	0.0015612982549830696	0.0	0.07496005826481214	2.2177532031009514E-4
58-59	1.951622818728837E-4	0.0015612982549830696	0.0	0.0813915425538049	2.3951734593490273E-4
60-61	2.3064633312249892E-4	0.0016145243318574924	0.0	0.08798270507342093	2.6613038437211416E-4
62-63	2.4838835874730654E-4	0.0016500083831071078	0.0	0.09428112417022763	2.6613038437211416E-4
64-65	2.4838835874730654E-4	0.0017919445881055685	0.0	0.09994083034454126	2.6613038437211416E-4
66-67	2.4838835874730654E-4	0.0018274286393551837	0.0	0.10585779589041461	2.75001397184518E-4
68-69	2.4838835874730654E-4	0.001907267754666818	0.0	0.1114376629494166	2.838724099969218E-4
70-71	2.572593715597103E-4	0.0019338807931040295	0.0	0.11707962709810542	3.0161443562172935E-4
72-73	2.838724099969218E-4	0.0019516228187288372	0.0	0.1217369088246174	3.370984868713446E-4
74-75	2.838724099969218E-4	0.0019693648443536447	0.0	0.1254095081289526	3.725825381209598E-4
76-77	2.838724099969218E-4	0.0020758169981024907	0.0	0.12896678426672653	3.725825381209598E-4
78-79	2.838724099969218E-4	0.002146785100601721	0.0	0.13125550557232668	3.725825381209598E-4
80-81	2.838724099969218E-4	0.0021645271262265287	0.0	0.13194744457169422	3.725825381209598E-4
82-83	2.838724099969218E-4	0.0021911401646637395	0.0	0.13255954445575008	3.725825381209598E-4
84-85	2.838724099969218E-4	0.002235495228725759	0.0	0.13280793281449738	3.725825381209598E-4
86-87	2.838724099969218E-4	0.0022887213056001815	0.0	0.13293212699387102	3.814535509333636E-4
88	3.19356461246537E-4	0.00244839953622345	0.0	0.1329409980066834	3.903245637457674E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6425	0.0	49.38606	1
GTATCAA	11315	0.0	35.818268	1
TCAACGC	13775	0.0	28.84657	4
ATCAACG	13920	0.0	28.51613	3
CAACGCA	14180	0.0	28.02267	5
TATCAAC	14790	0.0	27.26159	2
AACGCAG	14625	0.0	27.197561	6
ACGCAGA	17185	0.0	23.073233	7
CGCAGAG	17465	0.0	22.727194	8
GTACATG	15245	0.0	21.3334	1
TACATGG	15285	0.0	21.113897	2
ACATGGG	15510	0.0	20.29682	3
GCAGAGT	21010	0.0	18.832918	9
CATGGGG	11435	0.0	18.705704	4
GAGTACT	12805	0.0	17.827154	12-13
AGAGTAC	18725	0.0	17.624084	10-11
CAGAGTA	20715	0.0	16.967588	10-11
TATACCG	375	6.184564E-11	16.678343	5
GTGGTAT	3180	0.0	15.734284	1
TGGTATC	2955	0.0	15.521299	2
>>END_MODULE