FastQCFastQC Report
Fri 27 May 2016
SRR2139760_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139760_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10485954
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92600.042.4071271
GTATCAA153950.033.8383181
TCAACGC169850.029.9081844
ATCAACG173150.029.36233
CAACGCA177200.028.644065
AACGCAG184100.027.6149526
TATCAAC189450.027.1667772
ACGCAGA220950.023.0280237
CGCAGAG224250.022.6518928
CTTATAC124200.020.6186811
GTACATG248850.020.094561
TACATGG246600.019.7019252
ACATGGG254800.018.5924073
TATACCG7350.018.1879865
TATACAC161200.017.9852473
GCAGAGT287750.017.7402299
GAGTACT150100.017.47762112-13
TATGCCG82700.017.13053142-43
TTATACA164400.016.8982542
CATGGGG186600.016.8579734
Produced by FastQC (version 0.11.5)