##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139760_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10485954
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.293129456795253	33.0	32.0	33.0	25.0	34.0
2	30.853035880187917	32.0	31.0	33.0	25.0	33.0
3	30.93306054937872	33.0	31.0	33.0	27.0	33.0
4	32.4566847232021	33.0	33.0	33.0	32.0	34.0
5	32.49769682377016	33.0	33.0	33.0	32.0	34.0
6	36.84975625489107	38.0	37.0	38.0	35.0	38.0
7	36.837276417577264	38.0	37.0	38.0	35.0	38.0
8	37.279069315009394	38.0	38.0	38.0	36.0	38.0
9	37.3133248534182	38.0	38.0	38.0	37.0	38.0
10-11	37.35369795633282	38.0	38.0	38.0	37.0	38.0
12-13	37.38606172600032	38.0	38.0	38.0	37.0	38.0
14-15	37.40284493904894	38.0	38.0	38.0	37.0	38.0
16-17	37.358374068778105	38.0	38.0	38.0	37.0	38.0
18-19	37.34475470710629	38.0	38.0	38.0	37.0	38.0
20-21	37.40439235130794	38.0	38.0	38.0	37.0	38.0
22-23	37.42639429802115	38.0	38.0	38.0	37.0	38.0
24-25	37.429280490331365	38.0	38.0	38.0	37.0	38.0
26-27	37.33123603478132	38.0	38.0	38.0	37.0	38.0
28-29	37.2382843507651	38.0	38.0	38.0	37.0	38.0
30-31	37.32507478741244	38.0	38.0	38.0	37.0	38.0
32-33	37.34620582773809	38.0	38.0	38.0	37.0	38.0
34-35	37.30911436751781	38.0	38.0	38.0	37.0	38.0
36-37	37.317426698816135	38.0	38.0	38.0	37.0	38.0
38-39	37.30967388086114	38.0	38.0	38.0	37.0	38.0
40-41	37.28541178041729	38.0	38.0	38.0	37.0	38.0
42-43	37.28449506138449	38.0	38.0	38.0	37.0	38.0
44-45	37.22703962286681	38.0	38.0	38.0	37.0	38.0
46-47	37.21567333257369	38.0	38.0	38.0	37.0	38.0
48-49	37.203156545386875	38.0	38.0	38.0	37.0	38.0
50-51	37.20591683914493	38.0	38.0	38.0	37.0	38.0
52-53	37.189032765793726	38.0	38.0	38.0	36.5	38.0
54-55	37.135311257037614	38.0	38.0	38.0	36.0	38.0
56-57	37.137013163154776	38.0	38.0	38.0	36.0	38.0
58-59	37.14839677166429	38.0	38.0	38.0	36.0	38.0
60-61	37.15879303834669	38.0	38.0	38.0	36.0	38.0
62-63	37.082322650510136	38.0	38.0	38.0	36.0	38.0
64-65	37.11286928852903	38.0	38.0	38.0	36.0	38.0
66-67	37.13187467268696	38.0	38.0	38.0	36.0	38.0
68-69	37.09932839969484	38.0	38.0	38.0	36.0	38.0
70-71	37.048090351790336	38.0	38.0	38.0	36.0	38.0
72-73	37.05978425864186	38.0	38.0	38.0	36.0	38.0
74-75	37.081248820997246	38.0	38.0	38.0	36.0	38.0
76-77	37.01837981184952	38.0	38.0	38.0	36.0	38.0
78-79	37.04829274862688	38.0	38.0	38.0	36.0	38.0
80-81	37.028958148039095	38.0	38.0	38.0	36.0	38.0
82-83	36.97293900459937	38.0	38.0	38.0	36.0	38.0
84-85	37.00975463193698	38.0	38.0	38.0	36.0	38.0
86-87	37.03761864392917	38.0	38.0	38.0	36.0	38.0
88-89	36.94569206748672	38.0	38.0	38.0	36.0	38.0
90-91	36.91026015231483	38.0	38.0	38.0	35.5	38.0
92-93	36.969922413956695	38.0	38.0	38.0	36.0	38.0
94-95	36.95412293312226	38.0	38.0	38.0	36.0	38.0
96-97	36.89798680578639	38.0	38.0	38.0	35.0	38.0
98-99	36.90946843746603	38.0	38.0	38.0	35.0	38.0
100	35.344079076015646	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	13.0
19	24.0
20	63.0
21	199.0
22	595.0
23	1708.0
24	4192.0
25	8638.0
26	15175.0
27	24446.0
28	37151.0
29	53473.0
30	74549.0
31	100689.0
32	134400.0
33	184550.0
34	278093.0
35	516240.0
36	1548695.0
37	7503061.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.17070320926451	20.38512661795007	12.807542356184282	25.63662781660114
2	14.766399757084434	20.464387197037407	40.004537501044254	24.764675544833906
3	18.051194960420386	24.873807380806745	30.89230603147792	26.18269162729495
4	12.753706198047137	17.334217182843172	38.21959635886045	31.69248026024924
5	13.476713563604603	36.98918492179616	35.73539232005658	13.798709194542658
6	30.899633011879384	37.53110129432882	18.24154688515948	13.327718808632316
7	27.00662286896767	32.05911268413825	22.79474446685223	18.139519980041843
8	24.73659525876234	36.65006541131117	21.066685968677717	17.546653361248772
9	25.46866026686747	16.257643319816204	20.80552708890388	37.46816932441245
10-11	24.37834936144103	26.45586181285937	28.933957749576244	20.231831076123356
12-13	24.997320224750176	23.786953480818244	29.044186156071255	22.171540138360324
14-15	22.053644332218127	25.344150851701237	26.120165127560163	26.48203968852047
16-17	22.169256130629602	28.589558947140148	26.90672684621733	22.334458076012922
18-19	22.249654156407704	27.843155710963448	28.28464629923038	21.62254383339847
20-21	22.715556590824722	27.062358304486594	28.190429233246224	22.031655871442457
22-23	22.441050860038512	27.165493913974547	28.042420234243444	22.3510349917435
24-25	22.387000672114606	27.203292684403994	28.19483579067984	22.21487085280156
26-27	22.452348866277696	27.34301433387614	28.101639917830507	22.102996882015656
28-29	22.37388620108724	27.26565710002476	28.08276067674484	22.27769602214316
30-31	22.44362046440699	27.30993657133499	28.176996812104242	22.06944615215377
32-33	22.327349783483278	27.42645765380105	28.085018865698586	22.161173697017087
34-35	22.5638193262329	27.239489805134788	28.14734005863453	22.049350809997783
36-37	22.297930919478603	27.375777642640482	28.085917463311983	22.240373974568932
38-39	22.157615832632903	27.24960912703665	28.311190406163213	22.281584634167235
40-41	22.387026154146714	27.097804790782654	28.279420010679658	22.235749044390975
42-43	22.36571605013839	27.162436798551585	28.14013095892733	22.331716192382693
44-45	22.27459178137611	27.28554960642966	28.116022394038925	22.323836218155304
46-47	22.475495959277165	27.160792483493296	27.87740893134097	22.48630262588857
48-49	22.321107564412003	27.24844222897057	27.90726847445317	22.52318173216426
50-51	22.220806442036	27.319915898529967	27.998957320882123	22.460320338551902
52-53	22.39594412850047	27.286082254089184	28.00838498258655	22.309588634823797
54-55	22.336450551622853	27.225608148518965	27.908091357828102	22.529849942030076
56-57	22.432043329370455	27.33585776902951	27.748306584778938	22.4837923168211
58-59	22.231266576890825	27.488707455492218	27.972871434962563	22.307154532654398
60-61	22.415920469501234	27.63764688009278	27.713775167754374	22.232657482651614
62-63	22.23298099153146	27.837222710404415	27.676345715760004	22.253450582304122
64-65	22.211438387997546	27.80682592232559	27.65475490631488	22.326980783361982
66-67	22.22556841158531	27.84557533361908	27.555880571412267	22.372975683383338
68-69	22.25398546096605	27.762388190623895	27.579734653022147	22.40389169538791
70-71	22.340364223309216	27.494102358707416	27.621872902360607	22.54366051562276
72-73	22.39920004513779	27.37422668043609	27.61061440919623	22.615958865229885
74-75	22.30589193218516	27.450569994322855	27.624154831860785	22.619383241631198
76-77	22.528567177970558	27.34852342234277	27.49269682952404	22.630212570162634
78-79	22.480271241108806	27.393876628537594	27.47024543787151	22.65560669248209
80-81	22.50040637883505	27.427573166228015	27.411293760452395	22.66072669448454
82-83	22.522171711472126	27.40050636141055	27.491658775213807	22.585663151903518
84-85	22.54710489410242	27.45187823716132	27.471359954047454	22.529656914688807
86-87	22.519925843970555	27.420629345794893	27.52188456065474	22.537560249579812
88-89	22.52307480404141	27.406750193479063	27.501745814041445	22.56842918843808
90-91	22.64252170877467	27.416093410903187	27.480404771338137	22.460980108984007
92-93	22.625144796262816	27.41811301620724	27.51915942070972	22.43758276682022
94-95	22.54219346937654	27.48606622953161	27.52336232628766	22.448377974804195
96-97	22.641762296911892	27.552419170485326	27.574049105031378	22.2317694275714
98-99	23.689852476958002	29.470700711837118	27.806640798125887	19.03280601307899
100	30.130484946514773	40.66225116644017	29.20726388704505	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1109.0
1	1061.0
2	1540.5
3	2281.5
4	2829.5
5	3671.0
6	4547.5
7	5643.5
8	7074.0
9	8217.0
10	8594.5
11	8825.0
12	9769.0
13	11699.0
14	15158.5
15	18965.5
16	21595.0
17	23044.0
18	23428.5
19	23111.0
20	23014.0
21	24316.5
22	27824.0
23	34237.5
24	44530.5
25	58445.0
26	76640.0
27	97946.5
28	120386.0
29	145284.5
30	170023.0
31	194029.5
32	218724.0
33	243488.5
34	267890.5
35	290245.5
36	314665.5
37	338751.5
38	359274.5
39	377597.0
40	395826.0
41	414061.0
42	429951.5
43	454775.5
44	474834.5
45	478663.5
46	476762.5
47	472797.0
48	469202.0
49	461903.0
50	453142.5
51	441381.0
52	423559.0
53	403721.0
54	381286.5
55	354744.5
56	326200.5
57	295223.5
58	262228.0
59	227342.0
60	191365.5
61	157149.5
62	125693.5
63	97816.5
64	74488.5
65	55991.0
66	41498.5
67	31593.0
68	24137.0
69	17816.0
70	13207.5
71	9648.0
72	6805.0
73	4631.0
74	3075.0
75	2066.0
76	1421.5
77	918.5
78	575.5
79	383.5
80	248.0
81	144.5
82	102.5
83	72.0
84	46.0
85	28.5
86	20.0
87	17.5
88	9.5
89	2.5
90	3.0
91	4.0
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	5.7219400352128194E-5
3	0.0
4	5.7219400352128194E-5
5	0.003890919223944717
6	5.340477366198631E-4
7	1.4304850088032046E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0010735928323316155
30-31	0.0
32-33	0.0
34-35	9.618839998676448E-6
36-37	0.0
38-39	0.0
40-41	0.0
42-43	9.912493989930988E-6
44-45	5.001974029050565E-6
46-47	3.5381964225700334E-5
48-49	1.2258357761090635E-4
50-51	5.674552351330726E-4
52-53	2.3942759316609107E-4
54-55	3.886354180682488E-4
56-57	5.726876358012069E-4
58-59	3.909802463925377E-4
60-61	5.951323046741962E-5
62-63	4.262203083594644E-4
64-65	9.936746535843142E-5
66-67	1.5632895833716013E-4
68-69	6.612718563771785E-4
70-71	1.087358993861E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.001160480428751226
90-91	0.0
92-93	3.247826294817404E-5
94-95	9.20403444386364E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	178.0
20-21	316.0
22-23	519.0
24-25	830.0
26-27	3173.0
28-29	11302.0
30-31	27370.0
32-33	35137.0
34-35	50055.0
36-37	79459.0
38-39	86272.0
40-41	82169.0
42-43	88377.0
44-45	102206.0
46-47	104330.0
48-49	98765.0
50-51	88781.0
52-53	83915.0
54-55	91152.0
56-57	92523.0
58-59	93999.0
60-61	92475.0
62-63	91842.0
64-65	98564.0
66-67	107868.0
68-69	108825.0
70-71	118544.0
72-73	94095.0
74-75	90389.0
76-77	94492.0
78-79	97240.0
80-81	95767.0
82-83	89392.0
84-85	87283.0
86-87	90410.0
88-89	93947.0
90-91	93853.0
92-93	90791.0
94-95	103872.0
96-97	312757.0
98-99	2339131.0
100-101	4873589.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.08306547029426
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.96949807513852	79.21902756677095
2	6.482409908342124	11.290162529068828
3	1.4959354439580497	3.908119326165976
4	0.49048760541842656	1.7085265702008277
5	0.22327234391758322	0.9721620071540477
6	0.11256787602531247	0.5881653430658553
7	0.06291634752435264	0.38352638874304906
8	0.040423503196980425	0.28161620603530363
9	0.030137402024549557	0.23620116182475853
>10	0.08968631500574488	1.2106889516889228
>50	0.002330394054512383	0.13708792880038728
>100	3.014051526467761E-4	0.04582163998055436
>500	3.3380241181594406E-5	0.018894380500585537
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	9.536566725354699E-6	0.0	0.0	0.0
3	0.0	9.536566725354699E-6	0.0	0.0	0.0
4	0.0	9.536566725354699E-6	0.0	2.8609700176064094E-5	0.0
5	0.0	1.9073133450709398E-5	0.0	2.8609700176064094E-5	0.0
6	0.0	1.9073133450709398E-5	0.0	2.8609700176064094E-5	0.0
7	0.0	1.9073133450709398E-5	0.0	2.8609700176064094E-5	0.0
8	0.0	2.8609700176064094E-5	0.0	3.8146266901418796E-5	0.0
9	0.0	7.629253380283759E-5	0.0	5.721940035212819E-5	0.0
10-11	0.0	7.629253380283759E-5	0.0	6.67559670774829E-5	0.0
12-13	0.0	7.629253380283759E-5	0.0	9.536566725354698E-5	0.0
14-15	0.0	1.1920708406693372E-4	0.0	9.536566725354698E-5	1.9073133450709398E-5
16-17	0.0	1.335119341549658E-4	0.0	1.3828021751764312E-4	1.9073133450709398E-5
18-19	0.0	1.335119341549658E-4	0.0	1.7642648441906192E-4	1.9073133450709398E-5
20-21	0.0	1.335119341549658E-4	0.0	2.05036184595126E-4	1.9073133450709398E-5
22-23	0.0	1.4304850088032049E-4	0.0	2.574873015845769E-4	3.337798353874145E-5
24-25	0.0	1.6212163433102986E-4	0.0	3.0517013521135037E-4	4.768283362677349E-5
26-27	0.0	1.8119476778173925E-4	0.0	4.4821863609167083E-4	4.768283362677349E-5
28-29	0.0	1.859630511444166E-4	0.0	0.0012540585243841428	5.245111698945084E-5
30-31	0.0	2.3841416813386745E-4	0.0	0.003504688271567852	5.721940035212819E-5
32-33	0.0	2.479507348592221E-4	0.0	0.0077293873308999835	5.721940035212819E-5
34-35	0.0	3.0517013521135037E-4	0.0	0.013255827748243031	7.629253380283759E-5
36-37	0.0	3.576212522008012E-4	0.0	0.0221248348028229	7.629253380283759E-5
38-39	0.0	4.005358024648973E-4	0.0	0.04446901064032896	8.582910052819228E-5
40-41	0.0	4.4821863609167083E-4	0.0	0.06940236434376881	8.582910052819228E-5
42-43	0.0	4.768283362677349E-4	0.0	0.08342111743004021	8.582910052819228E-5
44-45	0.0	5.340477366198631E-4	0.0	0.10107807072203445	8.582910052819228E-5
46-47	0.0	5.721940035212819E-4	0.0	0.12023703327327204	9.059738389086963E-5
48-49	0.0	5.769622868839593E-4	0.0	0.13971070252644635	1.0490223397890168E-4
50-51	0.0	6.532548206867969E-4	0.0	0.15823548339044782	1.0490223397890168E-4
52-53	0.0	6.580231040494742E-4	0.0	0.20237071419538938	1.2874365079228842E-4
54-55	0.0	6.627913874121515E-4	0.0	0.25156509364813157	1.4304850088032049E-4
56-57	0.0	6.770962375001836E-4	0.0	0.3200805572864424	1.4304850088032049E-4
58-59	0.0	6.770962375001836E-4	0.0	0.37482045028997835	1.5258506760567518E-4
60-61	0.0	6.914010875882157E-4	0.0	0.40467467242370125	1.5258506760567518E-4
62-63	0.0	7.295473544896344E-4	0.0	0.42686149491023895	1.5258506760567518E-4
64-65	0.0	7.819984714790853E-4	0.0	0.4344430654568959	1.5258506760567518E-4
66-67	4.7682833626773495E-6	8.010716049297946E-4	0.0	0.4414190640164929	1.5258506760567518E-4
68-69	9.536566725354699E-6	8.153764550178267E-4	0.0	0.4483712211592765	1.5735335096835252E-4
70-71	9.536566725354699E-6	8.249130217431814E-4	0.0	0.4547130380316374	1.7165820105638456E-4
72-73	9.536566725354699E-6	8.296813051058587E-4	0.0	0.45948132139431475	1.7165820105638456E-4
74-75	9.536566725354699E-6	8.439861551938909E-4	0.0	0.4631910458504777	1.7165820105638456E-4
76-77	9.536566725354699E-6	8.630592886446002E-4	0.0	0.46668142927195755	1.7165820105638456E-4
78-79	9.536566725354699E-6	8.678275720072775E-4	0.0	0.46912279035364834	1.7165820105638456E-4
80-81	9.536566725354699E-6	8.678275720072775E-4	0.0	0.46991432539185274	1.7642648441906192E-4
82-83	9.536566725354699E-6	8.678275720072775E-4	0.0	0.4705151290955501	1.9073133450709395E-4
84-85	9.536566725354699E-6	8.678275720072775E-4	0.0	0.4707630798304093	1.9073133450709395E-4
86-87	9.536566725354699E-6	8.678275720072775E-4	0.0	0.4708346040808495	1.9073133450709395E-4
88	9.536566725354699E-6	8.86900705457987E-4	0.0	0.47083937236421214	1.9073133450709395E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9260	0.0	42.407127	1
GTATCAA	15395	0.0	33.838318	1
TCAACGC	16985	0.0	29.908184	4
ATCAACG	17315	0.0	29.3623	3
CAACGCA	17720	0.0	28.64406	5
AACGCAG	18410	0.0	27.614952	6
TATCAAC	18945	0.0	27.166777	2
ACGCAGA	22095	0.0	23.028023	7
CGCAGAG	22425	0.0	22.651892	8
CTTATAC	12420	0.0	20.618681	1
GTACATG	24885	0.0	20.09456	1
TACATGG	24660	0.0	19.701925	2
ACATGGG	25480	0.0	18.592407	3
TATACCG	735	0.0	18.187986	5
TATACAC	16120	0.0	17.985247	3
GCAGAGT	28775	0.0	17.740229	9
GAGTACT	15010	0.0	17.477621	12-13
TATGCCG	8270	0.0	17.130531	42-43
TTATACA	16440	0.0	16.898254	2
CATGGGG	18660	0.0	16.857973	4
>>END_MODULE