FastQCFastQC Report
Fri 27 May 2016
SRR2139759_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139759_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10088731
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93600.043.2773481
GTATCAA163350.035.9491961
TCAACGC175500.032.6696134
ATCAACG179500.031.9174353
CAACGCA182100.031.4855385
AACGCAG187400.030.6168526
TATCAAC194750.029.7521692
ACGCAGA224800.025.5424127
CGCAGAG231500.024.7844378
GTACATG240750.021.995761
TACATGG242850.021.18052
ACATGGG240100.020.646373
GCAGAGT293450.019.5817729
GAGTACT164400.018.25469412-13
CATGGGG174750.017.8443764
AGAGTAC251700.017.00607710-11
CAGAGTA286300.016.89734510-11
GTGGTAT57150.016.5435941
TGGTATC52050.016.2481352
GTACTTT192200.015.5807414-15
Produced by FastQC (version 0.11.5)