##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139759_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10088731
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.991567522218602	33.0	32.0	33.0	25.0	34.0
2	30.73643959780472	32.0	31.0	33.0	25.0	33.0
3	30.842403073290388	33.0	31.0	33.0	27.0	33.0
4	32.481969238747666	33.0	33.0	33.0	32.0	34.0
5	32.49482794218619	33.0	33.0	33.0	32.0	34.0
6	36.82887946957848	38.0	37.0	38.0	35.0	38.0
7	36.799200910401915	38.0	37.0	38.0	35.0	38.0
8	37.236247849209185	38.0	38.0	38.0	36.0	38.0
9	37.28146850183636	38.0	38.0	38.0	37.0	38.0
10-11	37.32658582134859	38.0	38.0	38.0	37.0	38.0
12-13	37.35883987787959	38.0	38.0	38.0	37.0	38.0
14-15	37.37935816704797	38.0	38.0	38.0	37.0	38.0
16-17	37.33164304807017	38.0	38.0	38.0	37.0	38.0
18-19	37.32004203501907	38.0	38.0	38.0	37.0	38.0
20-21	37.381010634681346	38.0	38.0	38.0	37.0	38.0
22-23	37.40076774811439	38.0	38.0	38.0	37.0	38.0
24-25	37.40441505194873	38.0	38.0	38.0	37.0	38.0
26-27	37.31079591615256	38.0	38.0	38.0	37.0	38.0
28-29	37.219083257881366	38.0	38.0	38.0	37.0	38.0
30-31	37.30390771396764	38.0	38.0	38.0	37.0	38.0
32-33	37.32670371019445	38.0	38.0	38.0	37.0	38.0
34-35	37.286548973832325	38.0	38.0	38.0	37.0	38.0
36-37	37.296325132216026	38.0	38.0	38.0	37.0	38.0
38-39	37.29321933415221	38.0	38.0	38.0	37.0	38.0
40-41	37.25412191419525	38.0	38.0	38.0	37.0	38.0
42-43	37.25118877723527	38.0	38.0	38.0	37.0	38.0
44-45	37.19265736633096	38.0	38.0	38.0	37.0	38.0
46-47	37.17455186432639	38.0	38.0	38.0	36.5	38.0
48-49	37.15818223398517	38.0	38.0	38.0	36.0	38.0
50-51	37.15637765779156	38.0	38.0	38.0	36.0	38.0
52-53	37.13870018378962	38.0	38.0	38.0	36.0	38.0
54-55	37.086580612385504	38.0	38.0	38.0	36.0	38.0
56-57	37.08671126937752	38.0	38.0	38.0	36.0	38.0
58-59	37.09573358639008	38.0	38.0	38.0	36.0	38.0
60-61	37.10598251595178	38.0	38.0	38.0	36.0	38.0
62-63	37.03251672053107	38.0	38.0	38.0	36.0	38.0
64-65	37.059932022146405	38.0	38.0	38.0	36.0	38.0
66-67	37.0785738406842	38.0	38.0	38.0	36.0	38.0
68-69	37.0493584374037	38.0	38.0	38.0	36.0	38.0
70-71	36.99597945915163	38.0	38.0	38.0	36.0	38.0
72-73	37.00970722810091	38.0	38.0	38.0	36.0	38.0
74-75	37.02919416210764	38.0	38.0	38.0	36.0	38.0
76-77	36.9649070586476	38.0	38.0	38.0	36.0	38.0
78-79	36.99049846938546	38.0	38.0	38.0	36.0	38.0
80-81	36.96778289831184	38.0	38.0	38.0	36.0	38.0
82-83	36.91433127669522	38.0	38.0	38.0	36.0	38.0
84-85	36.94470413269832	38.0	38.0	38.0	36.0	38.0
86-87	36.967038165298554	38.0	38.0	38.0	36.0	38.0
88-89	36.87603463089625	38.0	38.0	38.0	35.5	38.0
90-91	36.835792452991605	38.0	38.0	38.0	35.0	38.0
92-93	36.8889579359151	38.0	38.0	38.0	35.0	38.0
94-95	36.87334954564882	38.0	38.0	38.0	35.0	38.0
96-97	36.81777436022823	38.0	38.0	38.0	35.0	38.0
98-99	36.8283286394782	38.0	38.0	38.0	35.0	38.0
100	35.2325026218703	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	7.0
19	28.0
20	64.0
21	196.0
22	707.0
23	1976.0
24	4601.0
25	9120.0
26	15955.0
27	25759.0
28	38572.0
29	55992.0
30	77199.0
31	103565.0
32	137951.0
33	187820.0
34	283312.0
35	519582.0
36	1495811.0
37	7130512.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.027835909194124	20.75299658599283	12.849445584385192	25.36972192042785
2	14.610996852624462	20.738105308273404	40.04776422149761	24.603133617604527
3	17.9272298964062	25.2087304141621	31.05649263519862	25.807547054233083
4	12.563623023808168	17.403274666503847	38.94259352175627	31.090508787931714
5	13.273161527645543	37.261242811623525	35.91720536639594	13.548390294334991
6	30.482587366970964	38.00764440861992	18.512231678254075	12.997536546155041
7	26.749031196004097	32.436358676733086	23.192993209847664	17.62161691741516
8	24.60738620149551	36.804708144166	21.236218906024952	17.35168674831354
9	25.522882907671935	16.319297243627567	21.19161468374962	36.96620516495088
10-11	24.104751132724225	26.76712264406693	29.246478075389266	19.881648147819583
12-13	24.672860243770995	24.166607276970712	29.43374642459988	21.72678605465841
14-15	21.768649595276155	25.759964261114703	26.624131419501616	25.847254724107522
16-17	21.72317311265411	28.929956602074135	27.406747191495146	21.940123093776613
18-19	21.757369683065196	28.265447854640986	28.855809516578447	21.121372945715372
20-21	22.332708235677313	27.505970378122463	28.80932823197609	21.35199315422414
22-23	22.04038893325862	27.43984090734044	28.68407563721172	21.835694522189222
24-25	21.880770166217463	27.50020235001601	28.766752363282144	21.85227512048438
26-27	21.886103867636866	27.70123042291568	28.67714888790433	21.735516821543122
28-29	21.835374557958357	27.67431103586257	28.690116733308034	21.800197672871036
30-31	22.045294152774456	27.596855641908245	28.673694888812257	21.68415531650504
32-33	21.79482065669605	27.738805684356382	28.68774186938394	21.778631789563626
34-35	21.96773687341764	27.707731518951533	28.64454866931943	21.679982938311397
36-37	21.9215241711688	27.65609835263974	28.661342328995094	21.761035147196367
38-39	21.802460696853483	27.73367333224944	28.75038821184966	21.71347775904741
40-41	21.997805676133574	27.650586652328755	28.66139511229622	21.690212559241452
42-43	21.901175882688367	27.677260951511922	28.57653902742226	21.845024138377447
44-45	21.938112640053056	27.754793039495983	28.500692343013156	21.806401977437805
46-47	21.97448373393002	27.732628226188776	28.342459409013443	21.950428630867762
48-49	21.888509602661248	27.818001455631542	28.410817044290297	21.882671897416913
50-51	21.865930793366672	27.919666292449445	28.341966498399763	21.872436415784115
52-53	22.018845506704345	27.854318141070923	28.24186971268382	21.884966639540917
54-55	22.002926443173337	27.796453339027376	28.27785498908874	21.922765228710546
56-57	21.937340011313594	27.89079434887587	28.275942696775193	21.89592294303535
58-59	21.924729263917197	27.881553718520347	28.299091754407385	21.89462526315507
60-61	21.964801587430248	27.9155521259223	28.300580695992196	21.81906559065526
62-63	21.9524596671253	27.957453565508644	28.25784796064894	21.832238806717115
64-65	21.90943058994604	27.93549253099169	28.257179914645697	21.897896964416574
66-67	21.912458485267628	28.001751112732187	28.163154235939714	21.922636166060474
68-69	21.938537388223114	27.96147599743138	28.144439216676837	21.955547397668674
70-71	21.939800695055954	27.815161747531796	28.197354570239085	22.047682987173168
72-73	21.98217634068498	27.848862260346362	28.152796899214128	22.016164499754527
74-75	21.88308253440651	27.951572371557038	28.098785813140232	22.066559280896218
76-77	22.058543129083493	27.846115033411156	28.02554732948378	22.069794508021577
78-79	22.00638730920406	27.842578768774374	27.99271261470489	22.158321307316672
80-81	22.0200735385447	27.92021826173079	27.944937735535706	22.114770464188805
82-83	22.07041729551145	27.89502053368539	28.004745245130664	22.029816925672492
84-85	22.090430351540455	27.878135994658965	28.01350345616727	22.017930197633305
86-87	22.012061260069878	27.9001788615179	28.07179196883243	22.015967909579796
88-89	22.070368948384836	27.91431735269501	27.999452050624434	22.01586164829572
90-91	22.130909903287975	27.94675899901148	27.969504179827204	21.952826917873345
92-93	22.14545894409505	27.92013251557896	28.007547190037265	21.926861350288725
94-95	22.043465535038987	27.96819615971823	28.035199460435468	21.95313884480732
96-97	22.116098095963746	28.098386947022874	28.07578533747962	21.709729619533764
98-99	23.149077089267557	29.945933056289455	28.36588971126722	18.539100143175773
100	22.014164887641403	30.895092944877934	22.40403728429672	24.686704883183943
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1189.0
1	1129.5
2	1643.0
3	2426.0
4	2956.5
5	3813.0
6	4781.0
7	5994.5
8	7640.5
9	8909.5
10	9194.5
11	9347.5
12	10473.5
13	12835.5
14	16541.5
15	20366.5
16	23045.5
17	24623.5
18	24680.0
19	23731.0
20	23199.5
21	24108.5
22	27628.0
23	34140.5
24	44131.0
25	57991.5
26	76057.5
27	97450.0
28	119419.5
29	143338.0
30	168626.5
31	193063.5
32	218532.5
33	243571.0
34	267485.0
35	290405.5
36	314053.0
37	335392.5
38	352745.0
39	369179.0
40	384432.5
41	397436.5
42	407404.5
43	418622.0
44	426654.5
45	429965.0
46	430357.5
47	426491.0
48	420963.0
49	412472.5
50	401828.0
51	386712.0
52	367630.5
53	346658.5
54	323633.0
55	299896.0
56	273902.5
57	246920.5
58	219216.0
59	189143.0
60	157929.5
61	127626.5
62	100269.0
63	77207.0
64	57938.0
65	42857.5
66	31771.0
67	24144.5
68	18385.0
69	13520.5
70	10017.5
71	7357.5
72	5141.5
73	3468.5
74	2279.5
75	1481.0
76	972.0
77	590.5
78	387.5
79	254.0
80	164.5
81	103.0
82	64.5
83	43.0
84	30.0
85	20.5
86	12.0
87	6.5
88	4.5
89	4.5
90	4.0
91	2.5
92	2.5
93	2.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	7.929639515614006E-5
3	0.0
4	8.920844455065756E-5
5	0.0039251715602289326
6	4.956024697258753E-4
7	2.973614818355252E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	9.520577438889297E-4
30-31	0.0
32-33	0.0
34-35	4.986464740109447E-6
36-37	0.0
38-39	0.0
40-41	0.0
42-43	1.0181638910925066E-5
44-45	0.0
46-47	1.5482792965795567E-5
48-49	1.0396614737482099E-4
50-51	4.448716375227768E-4
52-53	2.2114764359024353E-4
54-55	2.807462128395308E-4
56-57	4.318146536356022E-4
58-59	3.703277518679546E-4
60-61	3.242299295999554E-5
62-63	3.5902933481819164E-4
64-65	9.857789880222923E-5
66-67	1.379087636440725E-4
68-69	6.059084298928092E-4
70-71	1.1203881114048956E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0010007286742921792
90-91	0.0
92-93	0.0
94-95	5.5283220854501685E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	138.0
20-21	316.0
22-23	447.0
24-25	677.0
26-27	2229.0
28-29	8083.0
30-31	18159.0
32-33	24177.0
34-35	33962.0
36-37	52972.0
38-39	56967.0
40-41	54738.0
42-43	59939.0
44-45	69713.0
46-47	70807.0
48-49	66635.0
50-51	59210.0
52-53	55608.0
54-55	60351.0
56-57	61600.0
58-59	63136.0
60-61	62404.0
62-63	60859.0
64-65	64554.0
66-67	69040.0
68-69	69146.0
70-71	69384.0
72-73	62733.0
74-75	62399.0
76-77	65494.0
78-79	67627.0
80-81	66248.0
82-83	62592.0
84-85	61772.0
86-87	64187.0
88-89	66811.0
90-91	67936.0
92-93	67408.0
94-95	82093.0
96-97	302391.0
98-99	702501.0
100-101	7071288.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.74944087958349
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.61878153245027	78.61128631129188
2	6.733327947961381	11.682248694890461
3	1.5186876408870298	3.952359111530506
4	0.5496372237269601	1.9072288737968135
5	0.23688769214977196	1.0274937422623809
6	0.11969627661680979	0.6230151043125728
7	0.0702848558762145	0.4268020364694602
8	0.04246637610253248	0.29471475064614394
9	0.024902828351334533	0.19442757922186596
>10	0.08336741657585257	1.1094183216808322
>50	0.0015709429340058437	0.09367508850283783
>100	3.545050680001089E-4	0.05245236410124772
>500	3.476129983230249E-5	0.024878021293021673
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.9824098789035012E-5	0.0	0.0	9.912049394517506E-6
3	0.0	2.973614818355252E-5	0.0	9.912049394517506E-6	9.912049394517506E-6
4	0.0	8.920844455065756E-5	0.0	9.912049394517506E-6	9.912049394517506E-6
5	0.0	9.912049394517507E-5	0.0	9.912049394517506E-6	9.912049394517506E-6
6	9.912049394517506E-6	9.912049394517507E-5	0.0	9.912049394517506E-6	9.912049394517506E-6
7	9.912049394517506E-6	9.912049394517507E-5	0.0	9.912049394517506E-6	9.912049394517506E-6
8	9.912049394517506E-6	9.912049394517507E-5	0.0	2.973614818355252E-5	9.912049394517506E-6
9	9.912049394517506E-6	1.9824098789035014E-4	0.0	5.947229636710504E-5	1.9824098789035012E-5
10-11	3.9648197578070025E-5	1.9824098789035014E-4	0.0	8.920844455065756E-5	2.973614818355252E-5
12-13	3.9648197578070025E-5	1.9824098789035014E-4	0.0	8.920844455065756E-5	2.973614818355252E-5
14-15	5.947229636710504E-5	2.973614818355252E-4	0.0	8.920844455065756E-5	2.973614818355252E-5
16-17	7.929639515614005E-5	3.171855806245602E-4	0.0	9.912049394517507E-5	2.973614818355252E-5
18-19	7.929639515614005E-5	3.320536547163365E-4	0.0	1.2390061743146883E-4	4.460422227532878E-5
20-21	7.929639515614005E-5	3.4692172880811274E-4	0.0	1.8337291379857387E-4	4.9560246972587536E-5
22-23	7.929639515614005E-5	3.8656992638618274E-4	0.0	2.6762533365197265E-4	5.451627166984629E-5
24-25	9.416446924791631E-5	4.1135004987247653E-4	0.0	3.072735312300427E-4	5.947229636710504E-5
26-27	9.912049394517507E-5	4.361301733587703E-4	0.0	3.717018522944065E-4	5.947229636710504E-5
28-29	1.0903254333969257E-4	4.41086198056029E-4	0.0	7.23579605799778E-4	6.442832106436379E-5
30-31	1.0903254333969257E-4	5.055145191203928E-4	0.0	0.001888245409655585	6.938434576162255E-5
32-33	1.0903254333969257E-4	5.055145191203928E-4	0.0	0.004068896276449437	6.938434576162255E-5
34-35	1.1894459273421008E-4	6.145470624600854E-4	0.0	0.006789753835244492	7.929639515614005E-5
36-37	1.2885664212872758E-4	7.03755507010743E-4	0.0	0.0116912622608334	8.920844455065756E-5
38-39	1.387686915232451E-4	7.087115317080017E-4	0.0	0.0249684524247896	8.920844455065756E-5
40-41	1.387686915232451E-4	7.681838280751067E-4	0.0	0.03915755113304141	9.912049394517507E-5
42-43	1.585927903122801E-4	7.979199762586593E-4	0.0	0.047424200328069016	9.912049394517507E-5
44-45	1.6354881500953887E-4	9.019964949010932E-4	0.0	0.05757909493275219	1.1398856803695133E-4
46-47	1.7346086440405637E-4	9.961609641490094E-4	0.0	0.06814533958730785	1.1894459273421008E-4
48-49	1.8337291379857387E-4	0.0010110290382407856	0.0	0.07877601256292788	1.1894459273421008E-4
50-51	1.9824098789035014E-4	0.0011101495321859608	0.0	0.08943642168673147	1.1894459273421008E-4
52-53	1.9824098789035014E-4	0.0011200615815804783	0.0	0.11589168152069869	1.3381266682598633E-4
54-55	2.0815303728486764E-4	0.0011299736309749958	0.0	0.14940927654825964	1.486807409177626E-4
56-57	2.0815303728486764E-4	0.0011597097791585483	0.0	0.1965262033450986	1.5363676561502135E-4
58-59	2.0815303728486764E-4	0.0011795338779475833	0.0	0.23239791010385746	1.585927903122801E-4
60-61	2.3788918546842016E-4	0.0011993579767366183	0.0	0.24929299829681256	1.6354881500953887E-4
62-63	2.4780123486293766E-4	0.0012191820755256533	0.0	0.26080088764384735	1.7841688910131512E-4
64-65	2.527572595601964E-4	0.0012984784706817933	0.0	0.2656429237730692	1.7841688910131512E-4
66-67	2.5771328425745516E-4	0.001303434495379052	0.0	0.2697415561977021	1.7841688910131512E-4
68-69	2.6762533365197265E-4	0.0013183025694708285	0.0	0.27407312178310633	1.8337291379857387E-4
70-71	2.6762533365197265E-4	0.0013183025694708285	0.0	0.27812714998546395	1.8832893849583262E-4
72-73	2.775373830464902E-4	0.0013183025694708285	0.0	0.2814427305079301	2.3788918546842016E-4
74-75	2.775373830464902E-4	0.0013629067917461572	0.0	0.2839752591282293	2.3788918546842016E-4
76-77	2.775373830464902E-4	0.0014174230634160035	0.0	0.2864136232792806	2.3788918546842016E-4
78-79	3.072735312300427E-4	0.001447159211599556	0.0	0.287895474663761	2.3788918546842016E-4
80-81	3.072735312300427E-4	0.0014570712609940735	0.0	0.2883662970100006	2.527572595601964E-4
82-83	3.072735312300427E-4	0.0014768953597831085	9.912049394517506E-6	0.28882225128214833	2.874494324410077E-4
84-85	3.1222955592730145E-4	0.0015115875326639197	9.912049394517506E-6	0.2890353603441305	2.874494324410077E-4
86-87	3.171855806245602E-4	0.0015363676561502134	9.912049394517506E-6	0.2890948326404976	2.874494324410077E-4
88	3.171855806245602E-4	0.0016354881500953886	9.912049394517506E-6	0.2891047446898921	2.874494324410077E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9360	0.0	43.277348	1
GTATCAA	16335	0.0	35.949196	1
TCAACGC	17550	0.0	32.669613	4
ATCAACG	17950	0.0	31.917435	3
CAACGCA	18210	0.0	31.485538	5
AACGCAG	18740	0.0	30.616852	6
TATCAAC	19475	0.0	29.752169	2
ACGCAGA	22480	0.0	25.542412	7
CGCAGAG	23150	0.0	24.784437	8
GTACATG	24075	0.0	21.99576	1
TACATGG	24285	0.0	21.1805	2
ACATGGG	24010	0.0	20.64637	3
GCAGAGT	29345	0.0	19.581772	9
GAGTACT	16440	0.0	18.254694	12-13
CATGGGG	17475	0.0	17.844376	4
AGAGTAC	25170	0.0	17.006077	10-11
CAGAGTA	28630	0.0	16.897345	10-11
GTGGTAT	5715	0.0	16.543594	1
TGGTATC	5205	0.0	16.248135	2
GTACTTT	19220	0.0	15.58074	14-15
>>END_MODULE