FastQCFastQC Report
Fri 27 May 2016
SRR2139758_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139758_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9038214
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82900.044.0988581
GTATCAA137900.032.8254781
TCAACGC149850.029.776064
ATCAACG152250.029.2217353
CAACGCA156700.028.5019365
AACGCAG163500.027.3947286
TATCAAC169600.026.4358632
ACGCAGA196350.022.81157
CGCAGAG200650.022.3011578
GTACATG200750.021.8614141
TACATGG198150.021.6261062
CTTATAC126750.021.427851
ACATGGG202550.020.3687023
TATACAC166600.019.1228713
GCAGAGT251800.017.7538389
GAGTACT146250.017.6563212-13
CATGGGG149250.017.3308774
TTATACA168400.017.0753572
TATGCCG88850.016.59901842-43
CAGAGTA246250.016.37709210-11
Produced by FastQC (version 0.11.5)