##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139758_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9038214
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.18204702831776	33.0	32.0	33.0	25.0	34.0
2	30.825644092959074	32.0	31.0	33.0	25.0	33.0
3	30.902832683536815	33.0	31.0	33.0	27.0	33.0
4	32.47994271877165	33.0	33.0	33.0	32.0	34.0
5	32.48264856309001	33.0	33.0	33.0	32.0	34.0
6	36.8065592383628	38.0	37.0	38.0	35.0	38.0
7	36.786618019887555	38.0	37.0	38.0	34.0	38.0
8	37.22678197263309	38.0	38.0	38.0	36.0	38.0
9	37.271383704789464	38.0	38.0	38.0	37.0	38.0
10-11	37.317358495826724	38.0	38.0	38.0	37.0	38.0
12-13	37.346086018764325	38.0	38.0	38.0	37.0	38.0
14-15	37.36605063788045	38.0	38.0	38.0	37.0	38.0
16-17	37.31872071185745	38.0	38.0	38.0	37.0	38.0
18-19	37.3085495098921	38.0	38.0	38.0	37.0	38.0
20-21	37.37077865432481	38.0	38.0	38.0	37.0	38.0
22-23	37.390976542582166	38.0	38.0	38.0	37.0	38.0
24-25	37.39385303035937	38.0	38.0	38.0	37.0	38.0
26-27	37.299580073821375	38.0	38.0	38.0	37.0	38.0
28-29	37.20528567573808	38.0	38.0	38.0	36.5	38.0
30-31	37.28803241253067	38.0	38.0	38.0	37.0	38.0
32-33	37.31087186087817	38.0	38.0	38.0	37.0	38.0
34-35	37.26785512588002	38.0	38.0	38.0	37.0	38.0
36-37	37.27663232797443	38.0	38.0	38.0	37.0	38.0
38-39	37.26029044071724	38.0	38.0	38.0	37.0	38.0
40-41	37.24091500660013	38.0	38.0	38.0	37.0	38.0
42-43	37.240348694119135	38.0	38.0	38.0	37.0	38.0
44-45	37.18012696676564	38.0	38.0	38.0	36.5	38.0
46-47	37.163026990334416	38.0	38.0	38.0	36.5	38.0
48-49	37.14642628611328	38.0	38.0	38.0	36.0	38.0
50-51	37.143706303570305	38.0	38.0	38.0	36.0	38.0
52-53	37.12572434583359	38.0	38.0	38.0	36.0	38.0
54-55	37.0729132131526	38.0	38.0	38.0	36.0	38.0
56-57	37.069820778160675	38.0	38.0	38.0	36.0	38.0
58-59	37.08037578429548	38.0	38.0	38.0	36.0	38.0
60-61	37.08752599586238	38.0	38.0	38.0	36.0	38.0
62-63	37.01317507654681	38.0	38.0	38.0	36.0	38.0
64-65	37.04009904748423	38.0	38.0	38.0	36.0	38.0
66-67	37.053735895996695	38.0	38.0	38.0	36.0	38.0
68-69	37.02105661386044	38.0	38.0	38.0	36.0	38.0
70-71	36.9706485404206	38.0	38.0	38.0	36.0	38.0
72-73	36.98223081521457	38.0	38.0	38.0	36.0	38.0
74-75	37.002399860368655	38.0	38.0	38.0	36.0	38.0
76-77	36.9383266108584	38.0	38.0	38.0	36.0	38.0
78-79	36.96305854127746	38.0	38.0	38.0	36.0	38.0
80-81	36.93837116607507	38.0	38.0	38.0	36.0	38.0
82-83	36.88422391782731	38.0	38.0	38.0	35.0	38.0
84-85	36.91578730217154	38.0	38.0	38.0	35.5	38.0
86-87	36.93767250200479	38.0	38.0	38.0	36.0	38.0
88-89	36.84301433585198	38.0	38.0	38.0	35.5	38.0
90-91	36.799875099441934	38.0	38.0	38.0	35.0	38.0
92-93	36.85448882499637	38.0	38.0	38.0	35.0	38.0
94-95	36.833621584709554	38.0	38.0	38.0	35.0	38.0
96-97	36.776951051514715	38.0	38.0	38.0	35.0	38.0
98-99	36.78435705437555	38.0	38.0	38.0	35.0	38.0
100	35.15401702455846	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	10.0
19	20.0
20	65.0
21	180.0
22	635.0
23	1681.0
24	4175.0
25	8465.0
26	14517.0
27	23619.0
28	35842.0
29	52053.0
30	70904.0
31	96184.0
32	126505.0
33	173778.0
34	259596.0
35	472687.0
36	1350794.0
37	6346503.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.736422040903214	20.79835684350913	12.865495329055054	25.599725786532606
2	14.534995713198425	20.70147320148786	40.16778991673902	24.595741168574698
3	17.739013482088385	25.24753231114023	31.32135397546462	25.692100231306757
4	12.434175303054996	17.63764975170418	38.76302511636429	31.165149828876533
5	13.135449475287054	37.21011775530892	36.12104766884852	13.533385100555506
6	30.265640057666545	38.18173369166546	18.44677628325203	13.105849967415969
7	26.441206757048164	32.42086621826652	23.1758467138449	17.962080310840413
8	24.44320304874392	36.98135494468266	21.307826966699395	17.26761503987403
9	25.27597819657733	16.2915372439732	21.078898994867792	37.35358556458168
10-11	23.937494730706753	26.75773111811692	29.34667180927559	19.958102341900734
12-13	24.563879545228737	24.12192829247017	29.42390498830853	21.89028717399256
14-15	21.689589337008396	25.757168396322548	26.46029956803413	26.09294269863493
16-17	21.761959829674314	29.055104249578513	27.249443308158007	21.93349261258917
18-19	21.792081931231102	28.224021914063997	28.683177893331578	21.30071826137332
20-21	22.236776716605277	27.471659166811563	28.64277840048369	21.64878571609947
22-23	21.994234950310844	27.600356571581834	28.502883437683764	21.902525040423562
24-25	21.981975751526733	27.587684112569395	28.62103821176085	21.80930192414302
26-27	22.036644448017643	27.796087668954115	28.50575610671636	21.66151177631188
28-29	21.796098869641657	27.607380776607055	28.57757914216049	22.01894121159079
30-31	22.115741311848215	27.649017210550014	28.58958411541191	21.64565736218986
32-33	22.026032110601207	27.60459935050223	28.68181272962323	21.687555809273334
34-35	21.815497115184616	27.673561581014155	28.80135858223179	21.709582721569443
36-37	21.98741087663035	27.688717169312927	28.59659438665941	21.72727756739731
38-39	21.712071551589844	27.643950975202536	28.819798544274988	21.824178928932632
40-41	21.899363775243003	27.56212412439565	28.755710660042144	21.7828014403192
42-43	21.909344507771525	27.579859771339176	28.705699072810752	21.805096648078543
44-45	21.832617778190098	27.757830635729242	28.619627444979045	21.78992414110161
46-47	22.02730050814477	27.59863700303405	28.356580561163945	22.017481927657233
48-49	21.89161642922913	27.64926648779013	28.432190088252096	22.026926994728633
50-51	21.783548799218693	27.81655252471531	28.47389095991287	21.92600771615313
52-53	21.920154497632378	27.82952245155891	28.476462395280056	21.773860655528658
54-55	21.872282793727436	27.828600960437537	28.321202197519867	21.97791404831516
56-57	21.959735302045065	28.022456428372493	28.1516464048864	21.86616186469604
58-59	21.694412011573803	28.094150003176445	28.357548094230616	21.853889891019136
60-61	22.01288856523791	28.23807743571602	28.202084240777776	21.5469497582683
62-63	21.691006312103543	28.392311210324923	28.15018633692651	21.766496140645028
64-65	21.678499127599142	28.36583227029548	28.109941129304794	21.84572747280058
66-67	21.702352684260088	28.3411960284665	28.25329277671564	21.70315851055778
68-69	21.710247526688303	28.41162788878896	28.094562233605757	21.78356235091698
70-71	21.757942983789242	28.164522741323932	27.9989625882438	22.078571686643034
72-73	21.821362260142806	28.017974016697742	28.048850145027814	22.111813578131635
74-75	21.803058818035954	28.08931572178135	28.17236873324573	21.935256726936966
76-77	21.95337811490133	27.778190453374485	28.122303564687485	22.1461278670367
78-79	21.89016113844186	27.817354211250883	27.903585943378022	22.38889870692923
80-81	22.113389304861826	28.050389534552263	27.847214006569082	21.989007154016832
82-83	22.15699637475357	27.813865298399058	27.926630955548404	22.10250737129897
84-85	21.963918751530596	27.804046753382078	27.95103084067545	22.281003654411876
86-87	21.896940431939257	28.023608894398805	27.993439034035315	22.08601163962662
88-89	22.155080383501804	27.970458649636658	27.93231917540333	21.9421417914582
90-91	22.203561971331666	28.027574027640206	27.91160098407861	21.857263016949517
92-93	22.011959923970092	27.775931319605057	27.968457769570097	22.24365098685475
94-95	22.12080314794346	28.062833642475237	27.966928955689223	21.849434253892074
96-97	22.38011666287094	27.96505851738485	28.037638988307855	21.617185831436355
98-99	23.120926950113436	29.96208622982236	28.41032583478781	18.506660985276394
100	22.014071645677653	30.879137221281233	22.48215557455336	24.624635558487757
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1224.0
1	1082.5
2	1416.0
3	2087.5
4	2626.0
5	3478.0
6	4269.0
7	5221.5
8	6626.0
9	7701.5
10	8029.5
11	8411.0
12	9464.5
13	11414.0
14	14703.5
15	18179.5
16	20624.5
17	22066.5
18	22355.0
19	21856.5
20	21679.5
21	22776.5
22	26231.5
23	32679.5
24	42235.0
25	54648.0
26	70700.5
27	89428.0
28	109636.0
29	132080.5
30	154669.0
31	176792.0
32	199172.5
33	220263.5
34	240421.0
35	259598.0
36	279333.0
37	297623.0
38	313460.0
39	328431.0
40	342699.0
41	355760.0
42	364594.5
43	372000.0
44	377230.5
45	383089.5
46	400394.0
47	405888.5
48	396495.5
49	384999.5
50	366354.5
51	346896.5
52	327972.5
53	309129.5
54	288901.0
55	267886.5
56	245908.0
57	221706.5
58	196306.5
59	169362.5
60	140803.5
61	114695.0
62	91415.5
63	70727.5
64	53283.0
65	39527.0
66	29611.5
67	22780.5
68	17540.5
69	12883.0
70	9318.5
71	6850.0
72	4981.0
73	3510.0
74	2416.0
75	1655.0
76	1109.0
77	718.0
78	467.5
79	296.5
80	185.5
81	128.0
82	98.0
83	67.0
84	43.5
85	32.0
86	22.0
87	13.5
88	7.0
89	3.0
90	3.5
91	3.0
92	2.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	7.744892962260022E-5
3	0.0
4	3.319239840968581E-5
5	0.0035405224970331524
6	4.8682184334205853E-4
7	7.744892962260022E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0010129597965666752
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	5.694425834744459E-6
44-45	5.73376774651288E-6
46-47	2.311613470950618E-5
48-49	8.736093958437741E-5
50-51	3.93045211638714E-4
52-53	1.712071895684522E-4
54-55	3.2679154104255174E-4
56-57	4.3675390616539767E-4
58-59	2.9478264201890733E-4
60-61	1.2102619563594061E-5
62-63	3.4762524388655267E-4
64-65	5.5310509513038894E-5
66-67	9.917852289086798E-5
68-69	4.3795999485709837E-4
70-71	6.316433882917824E-5
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	9.596028305969567E-4
90-91	0.0
92-93	2.770263630060657E-5
94-95	9.084521973152582E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	115.0
20-21	288.0
22-23	445.0
24-25	639.0
26-27	2310.0
28-29	7886.0
30-31	17399.0
32-33	23403.0
34-35	33294.0
36-37	51293.0
38-39	54953.0
40-41	52072.0
42-43	57480.0
44-45	67201.0
46-47	68324.0
48-49	63312.0
50-51	55497.0
52-53	53145.0
54-55	57472.0
56-57	59417.0
58-59	33611.0
60-61	64893.0
62-63	62012.0
64-65	67203.0
66-67	73534.0
68-69	74686.0
70-71	85176.0
72-73	62609.0
74-75	59279.0
76-77	62767.0
78-79	64340.0
80-81	62918.0
82-83	58361.0
84-85	57957.0
86-87	60565.0
88-89	63035.0
90-91	64151.0
92-93	62922.0
94-95	76416.0
96-97	297848.0
98-99	618325.0
100-101	6179661.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.51379846257538
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.39212517281834	78.20166099805093
2	6.9015630348955135	11.941608669554212
3	1.5551126297816706	4.036161019186111
4	0.5450531335927328	1.8861846780414744
5	0.24447041322405724	1.0575032029864306
6	0.1320943505201619	0.6856790411367647
7	0.06649930824652825	0.40271754260785764
8	0.04526170092176933	0.3132609337295451
9	0.02991338446702235	0.23291284636021542
>10	0.08641444841789524	1.1309748077023116
>50	0.0013042920488230364	0.07156535269233967
>100	1.494031514271595E-4	0.02025435374284947
>500	3.8727914049079336E-5	0.01951655420901319
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.2128265606457203E-5	0.0	0.0	0.0
3	0.0	2.2128265606457203E-5	0.0	1.1064132803228602E-5	0.0
4	0.0	5.532066401614301E-5	0.0	3.319239840968581E-5	0.0
5	0.0	6.638479681937161E-5	0.0	3.319239840968581E-5	0.0
6	5.532066401614301E-5	6.638479681937161E-5	0.0	3.319239840968581E-5	0.0
7	5.532066401614301E-5	6.638479681937161E-5	0.0	3.319239840968581E-5	0.0
8	5.532066401614301E-5	6.638479681937161E-5	0.0	3.319239840968581E-5	1.1064132803228602E-5
9	5.532066401614301E-5	1.5489785924520044E-4	0.0	7.744892962260022E-5	1.1064132803228602E-5
10-11	6.638479681937161E-5	1.5489785924520044E-4	0.0	1.0510926163067173E-4	1.1064132803228602E-5
12-13	6.638479681937161E-5	1.6042992564681475E-4	0.0	1.5489785924520044E-4	1.1064132803228602E-5
14-15	6.638479681937161E-5	2.1575058966295774E-4	0.0	1.5489785924520044E-4	3.319239840968581E-5
16-17	1.2723752723712894E-4	2.2128265606457205E-4	0.0	1.7149405845004332E-4	5.532066401614301E-5
18-19	1.3276959363874323E-4	2.3234678886780065E-4	0.0	2.1575058966295774E-4	5.532066401614301E-5
20-21	1.3276959363874323E-4	2.6553918727748645E-4	0.0	2.8213538648232936E-4	5.532066401614301E-5
22-23	1.3276959363874323E-4	2.7107125367910074E-4	0.0	3.2085985129362945E-4	7.74489296226002E-5
24-25	1.383016600403575E-4	2.8213538648232936E-4	0.0	3.4852018330170097E-4	9.957719522905742E-5
26-27	1.4383372644197182E-4	2.8766745288394364E-4	0.0	4.149049801210726E-4	9.957719522905742E-5
28-29	1.4383372644197182E-4	3.0426365208878654E-4	0.0	8.46406159446988E-4	9.957719522905742E-5
30-31	1.4383372644197182E-4	3.5405224970331525E-4	0.0	0.002445173349513521	9.957719522905742E-5
32-33	1.4383372644197182E-4	3.5405224970331525E-4	0.0	0.004635871644552784	9.957719522905742E-5
34-35	1.4936579284358613E-4	4.3703324572752977E-4	0.0	0.007595527169416436	9.957719522905742E-5
36-37	1.5489785924520044E-4	5.476745737598159E-4	0.0	0.013138657703833965	9.957719522905742E-5
38-39	1.5489785924520044E-4	5.86399038571116E-4	0.0	0.02861737949555078	1.1064132803228603E-4
40-41	1.5489785924520044E-4	6.583159017921019E-4	0.0	0.04592721526620193	1.2170546083551463E-4
42-43	1.6042992564681475E-4	7.081044994066305E-4	0.0	0.05472320084476867	1.2170546083551463E-4
44-45	1.6596199204842903E-4	7.744892962260021E-4	0.0	0.06668352840505878	1.2170546083551463E-4
46-47	1.6596199204842903E-4	8.076816946356879E-4	0.0	0.0794902621247959	1.2170546083551463E-4
48-49	1.6596199204842903E-4	8.187458274389165E-4	0.0	0.09110760156818593	1.2170546083551463E-4
50-51	1.6596199204842903E-4	8.851306242582882E-4	0.0	0.1030900573940825	1.2170546083551463E-4
52-53	1.6596199204842903E-4	8.961947570615168E-4	0.0	0.13498242019938894	1.3830166004035754E-4
54-55	1.6596199204842903E-4	9.18323022667974E-4	0.0	0.17075829361862865	1.5489785924520044E-4
56-57	1.6596199204842903E-4	9.293871554712026E-4	0.0	0.215473986342877	1.6042992564681475E-4
58-59	1.6596199204842903E-4	9.404512882744312E-4	0.0	0.405948564616859	1.6596199204842903E-4
60-61	1.7702612485165765E-4	9.791757530857315E-4	0.0	0.5745106278740468	1.6596199204842903E-4
62-63	1.8809025765488625E-4	0.0010013040186921886	0.0	0.5812099602864017	1.6596199204842903E-4
64-65	1.8809025765488625E-4	0.0010953491475196317	0.0	0.5870518224065064	1.6596199204842903E-4
66-67	1.9915439045811484E-4	0.0011064132803228603	0.0	0.5923736702848594	1.6596199204842903E-4
68-69	2.1021852326134346E-4	0.0011064132803228603	0.0	0.5976125371671881	1.6596199204842903E-4
70-71	2.1575058966295774E-4	0.0011174774131260889	0.0	0.6017007342379811	1.7149405845004332E-4
72-73	2.3234678886780065E-4	0.0011340736123309318	0.0	0.6051859360709981	2.1021852326134346E-4
74-75	2.3234678886780065E-4	0.0011617339443390031	0.0	0.6076532376861181	2.1021852326134346E-4
76-77	2.3787885526941496E-4	0.0011893942763470749	0.0	0.6102422447620736	2.1021852326134346E-4
78-79	2.4341092167102927E-4	0.0012059904755519176	0.0	0.6119848456785821	2.1021852326134346E-4
80-81	2.4341092167102927E-4	0.0012059904755519176	0.0	0.6125878409163581	2.1575058966295774E-4
82-83	2.4894298807264355E-4	0.0012059904755519176	0.0	0.6130746627597001	2.3234678886780065E-4
84-85	2.6553918727748645E-4	0.0012059904755519176	0.0	0.613318073681371	2.3787885526941496E-4
86-87	2.6553918727748645E-4	0.0012059904755519176	0.0	0.6134121188101986	2.4341092167102927E-4
88	2.766033200807151E-4	0.0013055676707809751	0.0	0.6134287150094034	2.4341092167102927E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8290	0.0	44.098858	1
GTATCAA	13790	0.0	32.825478	1
TCAACGC	14985	0.0	29.77606	4
ATCAACG	15225	0.0	29.221735	3
CAACGCA	15670	0.0	28.501936	5
AACGCAG	16350	0.0	27.394728	6
TATCAAC	16960	0.0	26.435863	2
ACGCAGA	19635	0.0	22.8115	7
CGCAGAG	20065	0.0	22.301157	8
GTACATG	20075	0.0	21.861414	1
TACATGG	19815	0.0	21.626106	2
CTTATAC	12675	0.0	21.42785	1
ACATGGG	20255	0.0	20.368702	3
TATACAC	16660	0.0	19.122871	3
GCAGAGT	25180	0.0	17.753838	9
GAGTACT	14625	0.0	17.65632	12-13
CATGGGG	14925	0.0	17.330877	4
TTATACA	16840	0.0	17.075357	2
TATGCCG	8885	0.0	16.599018	42-43
CAGAGTA	24625	0.0	16.377092	10-11
>>END_MODULE