FastQCFastQC Report
Fri 27 May 2016
SRR2139757_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139757_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6598155
Sequences flagged as poor quality0
Sequence length19-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76250.042.2283361
GTATCAA129100.035.732111
TCAACGC151100.029.8337214
ATCAACG155650.028.9616133
CAACGCA155750.028.9430185
TATCAAC163150.027.8450622
AACGCAG163250.027.7179346
GTACATG196050.024.1331181
ACGCAGA194500.023.2645387
TACATGG204600.023.1460322
CGCAGAG198500.022.7515968
ACATGGG208700.022.1455523
CATGGGG156150.019.3861944
GCAGAGT241500.018.682459
GAGTACT116750.018.51586312-13
AGAGTAC197750.017.88709310-11
CGTCTTC122600.016.94868348-49
CAGAGTA227300.016.92996410-11
TATGCCG132000.016.90010842-43
CCGTCTT133450.016.8923446-47
Produced by FastQC (version 0.11.5)