##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139757_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6598155
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.19483264639888	33.0	32.0	33.0	25.0	34.0
2	30.8839915097478	32.0	31.0	33.0	25.0	33.0
3	30.919673454170145	33.0	31.0	33.0	27.0	33.0
4	32.49057910885695	33.0	33.0	33.0	32.0	34.0
5	32.498753969859756	33.0	33.0	33.0	32.0	34.0
6	36.81502435150432	38.0	37.0	38.0	35.0	38.0
7	36.78525360498503	38.0	37.0	38.0	34.0	38.0
8	37.228433099858975	38.0	38.0	38.0	36.0	38.0
9	37.27797043264367	38.0	38.0	38.0	37.0	38.0
10-11	37.31873258509387	38.0	38.0	38.0	37.0	38.0
12-13	37.35033096373152	38.0	38.0	38.0	37.0	38.0
14-15	37.3718737283377	38.0	38.0	38.0	37.0	38.0
16-17	37.32277507879097	38.0	38.0	38.0	37.0	38.0
18-19	37.31305031482286	38.0	38.0	38.0	37.0	38.0
20-21	37.37797229281273	38.0	38.0	38.0	37.0	38.0
22-23	37.39961556739827	38.0	38.0	38.0	37.0	38.0
24-25	37.40396491310511	38.0	38.0	38.0	37.0	38.0
26-27	37.31269900527997	38.0	38.0	38.0	37.0	38.0
28-29	37.22372285347362	38.0	38.0	38.0	37.0	38.0
30-31	37.30678365754007	38.0	38.0	38.0	37.0	38.0
32-33	37.32313620333892	38.0	38.0	38.0	37.0	38.0
34-35	37.28024664482588	38.0	38.0	38.0	37.0	38.0
36-37	37.29037401735452	38.0	38.0	38.0	37.0	38.0
38-39	37.27266195717968	38.0	38.0	38.0	37.0	38.0
40-41	37.25821422261377	38.0	38.0	38.0	37.0	38.0
42-43	37.25642498588816	38.0	38.0	38.0	37.0	38.0
44-45	37.192875715051386	38.0	38.0	38.0	37.0	38.0
46-47	37.17359577933204	38.0	38.0	38.0	36.5	38.0
48-49	37.15484127160066	38.0	38.0	38.0	36.0	38.0
50-51	37.15439679014102	38.0	38.0	38.0	36.0	38.0
52-53	37.14064905775409	38.0	38.0	38.0	36.0	38.0
54-55	37.08981925074002	38.0	38.0	38.0	36.0	38.0
56-57	37.09346941291966	38.0	38.0	38.0	36.0	38.0
58-59	37.10992360778356	38.0	38.0	38.0	36.0	38.0
60-61	37.116942554483686	38.0	38.0	38.0	36.0	38.0
62-63	37.04603475378058	38.0	38.0	38.0	36.0	38.0
64-65	37.07796185129547	38.0	38.0	38.0	36.0	38.0
66-67	37.09404546932212	38.0	38.0	38.0	36.0	38.0
68-69	37.06253696395271	38.0	38.0	38.0	36.0	38.0
70-71	37.014664778719265	38.0	38.0	38.0	36.0	38.0
72-73	37.02562765619483	38.0	38.0	38.0	36.0	38.0
74-75	37.04725253576982	38.0	38.0	38.0	36.0	38.0
76-77	36.9852151781606	38.0	38.0	38.0	36.0	38.0
78-79	37.0121270824656	38.0	38.0	38.0	36.0	38.0
80-81	36.99032007943591	38.0	38.0	38.0	36.0	38.0
82-83	36.92674110033395	38.0	38.0	38.0	36.0	38.0
84-85	36.97870190835861	38.0	38.0	38.0	36.0	38.0
86-87	37.00613282371082	38.0	38.0	38.0	36.0	38.0
88-89	36.91315890852712	38.0	38.0	38.0	35.5	38.0
90-91	36.87573784797678	38.0	38.0	38.0	35.0	38.0
92-93	36.934239940727124	38.0	38.0	38.0	35.0	38.0
94-95	36.90268799147706	38.0	38.0	38.0	35.0	38.0
96-97	36.819536985456814	38.0	38.0	38.0	35.0	38.0
98-99	36.849804083782914	38.0	38.0	38.0	35.0	38.0
100	35.3033644511593	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	10.0
19	18.0
20	60.0
21	171.0
22	490.0
23	1322.0
24	2993.0
25	5910.0
26	10263.0
27	16422.0
28	25171.0
29	36008.0
30	50822.0
31	67952.0
32	90935.0
33	122064.0
34	182008.0
35	332722.0
36	951260.0
37	4701553.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.495554439081836	20.196039650478053	12.595248217115241	25.713157693324877
2	15.068297163856965	20.041690467526433	39.42430235379578	25.465710014820818
3	18.042664957097855	24.99709388457834	30.713100859255356	26.247140299068448
4	12.999594431895476	17.18435707452632	36.93963097546101	32.876417518117194
5	13.692805499480967	36.94113499545838	35.234990623473465	14.131068881587193
6	31.477649022305283	36.77244270832781	17.728647980645405	14.021260288721503
7	27.53899879845145	31.876925539150964	21.98779294566115	18.596282716736443
8	25.522270998483666	35.88237924086354	20.634237904383877	17.96111185626891
9	25.860911118335352	15.886986589432956	20.1999498344613	38.0521524577704
10-11	24.996805925292755	25.95341424989258	28.23681013859177	20.81296968622289
12-13	25.619631548516214	23.27205105063461	28.455189367330714	22.65312803351846
14-15	22.703808867781976	24.666721227373408	25.279665300375637	27.349804604468975
16-17	22.819545766960612	28.18793283880115	25.961886315189624	23.030635079048615
18-19	22.910995573762666	27.1059864462111	27.560590801519513	22.42242717850672
20-21	23.30393336731826	26.28381817676157	27.482624152520827	22.929624303399343
22-23	23.08068949560985	26.582216031924073	27.426401564344488	22.91069290812159
24-25	23.129367546225595	26.67676601634767	27.443993740313026	22.749872697113712
26-27	23.098612856889055	26.73295115197091	27.587485455219053	22.580950535920984
28-29	23.053434524117787	26.30956351787678	27.663940967610905	22.97306099039453
30-31	22.82117672063702	26.22092926164613	27.894268212146745	23.063625805570105
32-33	22.457173506568132	26.77729538985473	27.7312234436424	23.03430765993474
34-35	23.02381613213281	26.43541194158393	27.730457815017818	22.81031411126544
36-37	22.770197636640088	26.54584558552705	27.90889408864753	22.775062689185333
38-39	22.669500766374146	26.419781053511038	27.90192407520603	23.008794104908787
40-41	22.983070321205087	26.202523836879788	28.0620125150848	22.752393326830322
42-43	22.971244706220684	26.233766938446625	27.884755582134535	22.910232773198157
44-45	22.653471152226665	26.51512410562234	27.83342274510782	22.997981997043173
46-47	23.17100390163119	26.17608504279453	27.605133914499504	23.04777714107478
48-49	22.84978747705325	26.489231577886546	27.357605673601903	23.303375271458297
50-51	22.628455770222292	26.60049974435218	27.54243089533539	23.22861359009014
52-53	22.707240705312095	26.437191600518485	27.80829347012894	23.047274224040482
54-55	22.805521642798546	26.260021409097583	27.681007795428357	23.25344915267551
56-57	22.947344921945653	26.339674493444015	27.438927527968822	23.27405305664151
58-59	22.386717333108546	26.546487130953654	27.84868621034939	23.218109325588404
60-61	22.807034152885887	27.16702346303509	27.53535958583327	22.490582798245754
62-63	22.18553742469975	27.683432042758856	27.409706946051998	22.7213235864894
64-65	22.114723376345253	27.92358140740535	27.09141719891704	22.870278017332353
66-67	22.062847846534105	28.227819320543908	26.84899953697728	22.860333295944706
68-69	22.226189558556474	28.003222493802422	26.749723792165724	23.02086415547538
70-71	22.345873948211718	27.452201795954245	26.791657984814183	23.410266271019854
72-73	22.695042015232158	26.656337690704028	26.83927655002255	23.80934374404126
74-75	22.662981921991907	26.721883743150997	27.0869389233584	23.528195411498697
76-77	23.119946986582182	26.5673588321634	26.549974986529882	23.762719194724532
78-79	23.15478956462537	26.559812642051096	26.188176738641246	24.097221054682294
80-81	23.06684530718854	26.919499889215743	26.309579506283875	23.704075297311842
82-83	23.02766970939897	26.68361245334922	26.395598612774545	23.89311922447726
84-85	22.99802322918417	27.053292691832915	26.383819336760777	23.564864742222134
86-87	23.470829114808794	26.919857882661606	26.446448825064667	23.16286417746493
88-89	23.199253210513877	26.99398792367715	26.6787719529745	23.127986912834473
90-91	23.265783497034732	26.72119510997727	26.543384180240743	23.469637212747255
92-93	23.26191251055863	26.35526176258717	26.729192644246414	23.65363308260779
94-95	22.64841130572303	26.656881785334622	27.293513622936814	23.40119328600553
96-97	22.89309248555993	26.59763423018723	27.32722996049082	23.182043323762027
98-99	24.089802239958228	28.51752482679996	27.513821494327743	19.878851438914065
100	23.060258167756274	29.265459213177387	21.478385692333042	26.195896926733298
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	899.0
1	900.0
2	1419.0
3	2038.0
4	2445.5
5	3239.0
6	4057.5
7	4980.0
8	6264.0
9	7167.5
10	7185.0
11	7166.5
12	7910.5
13	9586.5
14	12332.5
15	14998.0
16	16813.0
17	17591.5
18	16938.5
19	15385.0
20	13758.0
21	12847.0
22	13330.5
23	15584.5
24	19511.0
25	25155.5
26	31948.0
27	40121.5
28	50315.5
29	62953.0
30	75819.5
31	89428.0
32	104820.0
33	118011.0
34	129327.5
35	141244.0
36	155084.0
37	169577.0
38	184778.5
39	197367.0
40	206696.5
41	216425.0
42	225998.0
43	236752.5
44	260079.0
45	291973.0
46	295612.0
47	287902.5
48	284907.0
49	277118.0
50	274815.0
51	273988.0
52	271949.0
53	266042.5
54	256426.5
55	243789.0
56	228356.0
57	210846.0
58	190067.5
59	165437.0
60	138722.5
61	113249.0
62	89934.0
63	68869.5
64	51122.5
65	37408.0
66	28272.5
67	22158.0
68	17182.0
69	12783.5
70	9368.0
71	6802.5
72	4875.0
73	3345.5
74	2242.5
75	1561.5
76	1018.0
77	585.5
78	382.5
79	250.5
80	144.5
81	89.0
82	68.5
83	49.5
84	32.5
85	23.5
86	17.5
87	10.0
88	6.0
89	5.0
90	5.0
91	4.0
92	3.5
93	5.0
94	4.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	6.062300749224594E-5
3	0.0
4	4.546725561918445E-5
5	0.004486102554426199
6	4.849840599379675E-4
7	4.546725561918445E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0012283351441166955
30-31	0.0
32-33	0.0
34-35	7.6228936229768656E-6
36-37	0.0
38-39	0.0
40-41	0.0
42-43	1.5502966221284536E-5
44-45	1.5595074514045784E-5
46-47	3.9254176919203436E-5
48-49	1.3437044128517583E-4
50-51	5.250557812102106E-4
52-53	2.152106246456497E-4
54-55	3.429144152607442E-4
56-57	5.026499225201048E-4
58-59	4.310524432770906E-4
60-61	4.01150049052628E-5
62-63	4.4426515877148246E-4
64-65	1.2200255977637418E-4
66-67	1.9692580762760938E-4
68-69	5.055841772375892E-4
70-71	1.0887198655950201E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0010254038404611944
90-91	0.0
92-93	2.742500335727749E-5
94-95	2.7654788228389094E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	91.0
20-21	230.0
22-23	301.0
24-25	433.0
26-27	1716.0
28-29	5676.0
30-31	11518.0
32-33	14593.0
34-35	19926.0
36-37	31807.0
38-39	19102.0
40-41	33847.0
42-43	36218.0
44-45	42971.0
46-47	43375.0
48-49	41758.0
50-51	20893.0
52-53	3167.0
54-55	3013.0
56-57	2959.0
58-59	21757.0
60-61	42736.0
62-63	41583.0
64-65	49856.0
66-67	59420.0
68-69	60696.0
70-71	78762.0
72-73	45662.0
74-75	40624.0
76-77	42851.0
78-79	44453.0
80-81	43920.0
82-83	40789.0
84-85	39853.0
86-87	41845.0
88-89	44273.0
90-91	44948.0
92-93	44371.0
94-95	54842.0
96-97	222430.0
98-99	515785.0
100-101	4643105.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.7937550021431
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.87516765182612	67.66426920725952
2	8.868689634074652	13.975947164346488
3	2.7483162232334073	6.49650465485605
4	1.1166241496970744	3.5193203872283063
5	0.5481968558963849	2.159724437822245
6	0.2868338027361525	1.3560427427475268
7	0.1721180254049484	0.9493277867647103
8	0.1059214090910423	0.6676756445921089
9	0.07008923745874548	0.4970334784150281
>10	0.20360667637519358	2.4185787525167073
>50	0.0036693046347420796	0.198513868048678
>100	7.296220752052879E-4	0.07920016419377675
>500	3.740749628052716E-5	0.017861711208858905
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.546725561918445E-5	0.0	0.0	0.0
3	0.0	4.546725561918445E-5	0.0	1.5155751873061484E-5	0.0
4	0.0	1.060902631114304E-4	0.0	3.031150374612297E-5	0.0
5	0.0	1.060902631114304E-4	0.0	3.031150374612297E-5	0.0
6	2.121805262228608E-4	1.060902631114304E-4	0.0	4.546725561918445E-5	0.0
7	2.121805262228608E-4	1.060902631114304E-4	0.0	4.546725561918445E-5	0.0
8	2.121805262228608E-4	1.2124601498449188E-4	0.0	4.546725561918445E-5	0.0
9	2.121805262228608E-4	2.576477818420452E-4	0.0	1.2124601498449188E-4	0.0
10-11	2.121805262228608E-4	2.576477818420452E-4	0.0	2.0460265028633004E-4	0.0
12-13	2.121805262228608E-4	2.576477818420452E-4	0.0	2.65225657778576E-4	0.0
14-15	2.121805262228608E-4	3.182707893342912E-4	0.0	2.7280353371510673E-4	1.5155751873061484E-5
16-17	3.4858229308041416E-4	3.2584866527082194E-4	0.0	3.4858229308041416E-4	3.031150374612297E-5
18-19	3.4858229308041416E-4	3.637380449534756E-4	0.0	4.3951680431878306E-4	4.546725561918445E-5
20-21	3.4858229308041416E-4	4.243610524457216E-4	0.0	5.152955636840905E-4	4.546725561918445E-5
22-23	3.5616016901694487E-4	4.622504321283753E-4	0.0	5.607628193032749E-4	4.546725561918445E-5
24-25	3.637380449534756E-4	5.380291914936827E-4	0.0	5.759185711763364E-4	4.546725561918445E-5
26-27	3.637380449534756E-4	5.607628193032749E-4	0.0	6.441194546051132E-4	6.062300749224594E-5
28-29	3.637380449534756E-4	5.607628193032749E-4	0.0	0.0012427716535910417	6.062300749224594E-5
30-31	3.637380449534756E-4	6.365415786685824E-4	0.0	0.0029174822355643357	6.062300749224594E-5
32-33	3.713159208900064E-4	6.365415786685824E-4	0.0	0.0061608131363994935	6.062300749224594E-5
34-35	3.940495486995986E-4	8.335663530183817E-4	0.0	0.010351378529300993	6.062300749224594E-5
36-37	3.940495486995986E-4	0.0010154353754951195	0.0	0.020164727867108306	6.062300749224594E-5
38-39	3.940495486995986E-4	0.0010154353754951195	0.0	0.16447021932646325	9.09345112383689E-5
40-41	3.940495486995986E-4	0.001091214134860427	0.0	0.31515628232437704	9.09345112383689E-5
42-43	4.092053005726601E-4	0.0011366813904796114	0.0	0.3362909783113613	9.851238717489965E-5
44-45	4.1678317650919083E-4	0.0013261282888928799	0.0	0.36442763166369996	1.1366813904796114E-4
46-47	4.243610524457216E-4	0.001470107931686964	0.0	0.3970806990742109	1.2124601498449188E-4
48-49	4.243610524457216E-4	0.0014852636835600255	0.0	0.42597514002020265	1.2124601498449188E-4
50-51	4.3951680431878306E-4	0.0016671327060367633	0.0	0.455907749969499	1.2124601498449188E-4
52-53	4.3951680431878306E-4	0.0016671327060367633	0.0	0.8517532552660554	1.2124601498449188E-4
54-55	4.3951680431878306E-4	0.0016671327060367633	0.0	1.3776881567650352	1.2124601498449188E-4
56-57	4.3951680431878306E-4	0.0016822884579098247	0.0	1.9539325766066424	1.2124601498449188E-4
58-59	4.3951680431878306E-4	0.0016822884579098247	0.0	2.543074541292225	1.2124601498449188E-4
60-61	5.228734396206212E-4	0.0016898663338463554	0.0	2.6986559121451377	1.2882389092102263E-4
62-63	6.516973305416439E-4	0.0017201778375924785	0.0	2.7050667951874425	1.3640176685755337E-4
64-65	6.516973305416439E-4	0.0018944689841326856	0.0	2.7107880915195235	1.3640176685755337E-4
66-67	6.74430958351236E-4	0.001962669867561462	0.0	2.7153878622129977	1.3640176685755337E-4
68-69	6.820088342877668E-4	0.0019854034953710542	0.0	2.719472337342787	1.439796427940841E-4
70-71	7.35053965843482E-4	0.002008137123180647	0.0	2.7233522098222913	1.6671327060367632E-4
72-73	8.487221048914432E-4	0.0020157149991171775	0.0	2.7260499336556965	1.970247743497993E-4
74-75	8.562999808279739E-4	0.002061182254736362	0.0	2.7282247840494804	1.970247743497993E-4
76-77	8.790336086375661E-4	0.002205161897530446	0.0	2.73047541320263	1.970247743497993E-4
78-79	8.941893605106276E-4	0.0022430512772130995	0.0	2.731998566265873	1.970247743497993E-4
80-81	8.941893605106276E-4	0.0022430512772130995	0.0	2.732544173333303	2.1218052622286077E-4
82-83	9.017672364471583E-4	0.0022430512772130995	0.0	2.7328548662467007	2.576477818420452E-4
84-85	9.09345112383689E-4	0.0022582070290861614	0.0	2.7329988458894947	2.576477818420452E-4
86-87	9.09345112383689E-4	0.0022733627809592228	0.0	2.733127669780416	2.576477818420452E-4
88	9.09345112383689E-4	0.0024552318034359605	0.0	2.733127669780416	2.576477818420452E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7625	0.0	42.228336	1
GTATCAA	12910	0.0	35.73211	1
TCAACGC	15110	0.0	29.833721	4
ATCAACG	15565	0.0	28.961613	3
CAACGCA	15575	0.0	28.943018	5
TATCAAC	16315	0.0	27.845062	2
AACGCAG	16325	0.0	27.717934	6
GTACATG	19605	0.0	24.133118	1
ACGCAGA	19450	0.0	23.264538	7
TACATGG	20460	0.0	23.146032	2
CGCAGAG	19850	0.0	22.751596	8
ACATGGG	20870	0.0	22.145552	3
CATGGGG	15615	0.0	19.386194	4
GCAGAGT	24150	0.0	18.68245	9
GAGTACT	11675	0.0	18.515863	12-13
AGAGTAC	19775	0.0	17.887093	10-11
CGTCTTC	12260	0.0	16.948683	48-49
CAGAGTA	22730	0.0	16.929964	10-11
TATGCCG	13200	0.0	16.900108	42-43
CCGTCTT	13345	0.0	16.89234	46-47
>>END_MODULE