FastQCFastQC Report
Fri 27 May 2016
SRR2139756_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139756_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5355399
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63250.052.5181851
GTATCAA121150.039.190271
TCAACGC141150.032.9204034
ATCAACG142100.032.8250543
CAACGCA145300.031.9197445
AACGCAG148800.031.2574126
TATCAAC150750.031.0876832
CGCAGAG172300.026.9193238
ACGCAGA175100.026.537497
GTACATG154550.026.3483491
TACATGG150650.026.3022582
ACATGGG156550.024.6938693
GAGTACT121500.022.5388812-13
GCAGAGT199500.022.009369
CATGGGG106600.021.5275464
GTGGTAT31400.020.822411
CAGAGTA187100.020.37212610-11
AGAGTAC169950.020.35858310-11
GTACTTT138400.019.61914814-15
TGGTATC32400.018.9608332
Produced by FastQC (version 0.11.5)