##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139756_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5355399
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.78001452366107	33.0	33.0	33.0	33.0	33.0
2	32.78015979761732	33.0	33.0	33.0	33.0	33.0
3	32.773015045190846	33.0	33.0	33.0	33.0	33.0
4	36.71594310713357	37.0	37.0	37.0	37.0	37.0
5	36.735567788693245	37.0	37.0	37.0	37.0	37.0
6	36.697741475471766	37.0	37.0	37.0	37.0	37.0
7	36.677253552909875	37.0	37.0	37.0	37.0	37.0
8	36.67893634069095	37.0	37.0	37.0	37.0	37.0
9	36.69330557816514	37.0	37.0	37.0	37.0	37.0
10-11	36.64247668567739	37.0	37.0	37.0	37.0	37.0
12-13	36.61624306610955	37.0	37.0	37.0	37.0	37.0
14-15	35.4748302600796	40.0	37.0	40.0	19.5	40.0
16-17	35.79173643271024	40.0	37.0	40.0	24.0	40.0
18-19	38.258045198873134	40.0	37.0	40.0	37.0	40.0
20-21	38.61943741773952	40.0	37.0	40.0	37.0	40.0
22-23	38.66355161117991	40.0	37.0	40.0	37.0	40.0
24-25	38.6981726553563	40.0	37.0	40.0	37.0	40.0
26-27	38.65490901709323	40.0	37.0	40.0	37.0	40.0
28-29	38.59834838006383	40.0	37.0	40.0	37.0	40.0
30-31	38.48824502200756	40.0	37.0	40.0	37.0	40.0
32-33	38.3221162215952	40.0	37.0	40.0	37.0	40.0
34-35	38.250796393536575	40.0	37.0	40.0	37.0	40.0
36-37	38.18417657544195	40.0	37.0	40.0	37.0	40.0
38-39	38.19261807968399	40.0	37.0	40.0	37.0	40.0
40-41	38.13999050576999	40.0	37.0	40.0	37.0	40.0
42-43	38.097689689447414	40.0	37.0	40.0	37.0	40.0
44-45	38.05358170147471	40.0	37.0	40.0	37.0	40.0
46-47	37.92366557531895	40.0	37.0	40.0	33.0	40.0
48-49	37.71435438994416	40.0	37.0	40.0	33.0	40.0
50-51	37.630591861354134	40.0	37.0	40.0	33.0	40.0
52-53	37.553653329828954	40.0	37.0	40.0	33.0	40.0
54-55	37.34845738054358	40.0	37.0	40.0	33.0	40.0
56-57	37.247138549678425	37.0	37.0	40.0	33.0	40.0
58-59	37.18973320967946	37.0	37.0	40.0	33.0	40.0
60-61	37.07061194985337	37.0	37.0	40.0	33.0	40.0
62-63	36.889530043491455	37.0	37.0	40.0	33.0	40.0
64-65	36.628099849538245	37.0	37.0	40.0	33.0	40.0
66-67	36.30822599716711	37.0	37.0	40.0	33.0	40.0
68-69	36.01671199738709	37.0	37.0	40.0	33.0	40.0
70-71	35.773262273294236	37.0	33.0	37.0	33.0	40.0
72-73	35.54998096687055	37.0	33.0	37.0	33.0	40.0
74-75	35.25935418585052	37.0	33.0	37.0	33.0	40.0
76-77	32.42826996725336	33.0	30.0	35.0	27.0	37.0
78-79	34.513123437042694	37.0	33.0	37.0	30.0	37.0
80-81	34.811293935033206	37.0	33.0	37.0	33.0	37.0
82-83	34.772785310548045	37.0	33.0	37.0	33.0	37.0
84-85	34.720946946768734	37.0	33.0	37.0	33.0	37.0
86-87	34.54729817075117	37.0	33.0	37.0	33.0	37.0
88-89	34.31818470447877	37.0	33.0	37.0	30.0	37.0
90-91	34.16041490208347	37.0	33.0	37.0	27.0	37.0
92-93	34.16824462055919	37.0	33.0	37.0	27.0	37.0
94-95	34.09232205174956	37.0	33.0	37.0	27.0	37.0
96-97	34.01749855139069	37.0	33.0	37.0	27.0	37.0
98-99	33.994092853364826	33.0	33.0	37.0	27.0	37.0
100	34.03032875963837	33.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	17.0
21	94.0
22	407.0
23	1302.0
24	3452.0
25	7045.0
26	13209.0
27	22118.0
28	34503.0
29	51577.0
30	73006.0
31	101660.0
32	143224.0
33	205503.0
34	304385.0
35	497916.0
36	937504.0
37	1776340.0
38	1166026.0
39	16111.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.52146310667048	19.571538927351632	12.91554560173761	23.991452364240274
2	15.069776717491685	21.480055152138938	40.44330847105185	23.00685965931753
3	18.676485444279024	26.040269885050687	30.17830690508144	25.104937765588854
4	12.388476468114632	17.645833006558647	38.89679372413345	31.06889680119327
5	13.21138036799506	38.169245310992764	35.5624922741503	13.056882046861876
6	30.318002449490695	37.92845313673174	18.684882302887235	13.068662110890337
7	26.861303891642805	32.75927713322574	22.983609624605002	17.395809350526452
8	25.007529784428762	36.38134152095857	21.313724710334377	17.297403984278294
9	26.211156255584317	16.341938294420267	20.957560771849117	36.489344678146296
10-11	24.367400076072762	26.935444025739258	28.873973722592844	19.823182175595132
12-13	25.189318293557584	24.492077994562123	29.06487453129076	21.25372918058953
14-15	22.355349965875483	25.451275148213696	26.785630079738983	25.407744806171838
16-17	21.760143361867154	28.980184296258788	27.419936404365014	21.839735937509044
18-19	21.70152961525369	28.087897839171273	29.254795020875196	20.95577752469984
20-21	22.474185337484567	27.378443690078026	29.20787355376361	20.9394974186738
22-23	22.123431387223707	27.3866384272558	29.057668401625	21.432261783895488
24-25	21.884284533807296	27.389877355537802	29.249565186446542	21.476272924208363
26-27	21.903140634919122	27.62373199950246	29.10310602006036	21.37002134551806
28-29	21.85488900004207	27.700129488264245	29.089552587661682	21.355428924031994
30-31	22.03565875326021	27.601346745391815	29.002252404625107	21.360742096722866
32-33	21.887622051717802	27.713452131759688	28.979867679649196	21.41905813687332
34-35	21.978709199968694	27.77879024307112	28.944277518009002	21.29822303895118
36-37	21.972200882568384	27.734603243857237	28.869012973360274	21.424182900214106
38-39	21.858978787078883	27.85832268334928	28.93918808058748	21.343510448984354
40-41	22.129514877748075	27.78257830583901	28.725619922307967	21.362286894104948
42-43	22.000108756241644	27.833933766312015	28.6007410582096	21.565216419236744
44-45	22.075865404204826	28.005384257710446	28.364236535053905	21.554513803030815
46-47	22.13088035545799	28.029658545006175	28.07775147004709	21.761709629488745
48-49	22.075091273067542	28.1149522925647	28.05330462194542	21.75665181242234
50-51	22.070690543126553	28.308653283244094	27.92636597348595	21.694290200143403
52-53	22.24668813132994	28.210704434905022	27.82113410822967	21.721473325535367
54-55	22.227024274697694	28.115018786686854	27.83224152200215	21.825715416613303
56-57	22.23722097777198	28.276805074833472	27.722912291153413	21.76306165624114
58-59	22.19292573770284	28.312419115273475	27.719223818705085	21.775431328318604
60-61	22.257271725722465	28.311893630511275	27.733508162349302	21.69732648141696
62-63	22.165872431167625	28.41089390219635	27.692066653190476	21.731167013445546
64-65	22.197483947537076	28.3463085781647	27.69226574241943	21.763941731878795
66-67	22.27167971520761	28.32253311147265	27.564511337795615	21.84127583552412
68-69	22.23149740738716	28.410693183750197	27.57977143193573	21.778037976926917
70-71	22.26993423655213	28.16092486788566	27.657184474804314	21.911956420757896
72-73	22.306650611572344	28.22137778059446	27.53210723112998	21.939864376703213
74-75	22.15018744612892	28.390900625065036	27.48847055720366	21.970441371602384
76-77	22.262812074820527	28.291680340008384	27.43336319959883	22.012144385572256
78-79	22.25763537637302	28.281204100725994	27.456148579318473	22.005011943582513
80-81	22.23272292076344	28.394960988491498	27.39069982846561	21.98161626227946
82-83	22.29380738567916	28.37892243437935	27.45342870074317	21.873841479198315
84-85	22.357055666142397	28.330333359907833	27.387113948435516	21.925497025514254
86-87	22.21070348689992	28.38677416540592	27.47334341931956	21.929178928374604
88-89	22.310821979862627	28.385227682241972	27.44434844551745	21.85960189237795
90-91	22.31938025661352	28.410225717839545	27.454085268320995	21.81630875722594
92-93	22.301281853268172	28.408322806873027	27.486921225983036	21.80347411387576
94-95	22.27355909111252	28.52560707459236	27.396042278250476	21.804791556044638
96-97	22.30021799332137	28.62839126261438	27.46492891082409	21.606461833240164
98-99	22.512077201906234	29.345097927184128	26.566723314623264	21.576101556286375
100	20.675181824967336	28.841139244022646	27.756119630912647	22.727559300097372
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1203.0
1	1052.0
2	1437.5
3	2210.5
4	3155.5
5	5126.0
6	6768.0
7	7705.0
8	8410.0
9	8389.5
10	7659.5
11	6944.0
12	7538.5
13	10322.0
14	16408.5
15	23892.5
16	30048.0
17	33826.0
18	33479.5
19	29923.5
20	25928.5
21	22501.5
22	20477.0
23	21208.5
24	24757.0
25	30468.5
26	38352.0
27	47679.0
28	58493.0
29	70480.0
30	82285.0
31	94453.5
32	107443.0
33	120279.5
34	132432.5
35	143283.5
36	155146.5
37	165660.0
38	173092.0
39	180679.0
40	188635.5
41	195472.5
42	199189.0
43	202248.0
44	205521.0
45	208792.0
46	210599.5
47	209810.5
48	207627.5
49	203569.0
50	198650.5
51	191684.5
52	182518.0
53	172449.5
54	161248.0
55	149080.5
56	136652.0
57	122999.5
58	108247.5
59	93957.5
60	78673.5
61	63834.5
62	50456.0
63	38723.5
64	29032.5
65	21396.0
66	15934.0
67	12202.0
68	9482.5
69	7086.5
70	5187.5
71	3795.0
72	2793.5
73	2043.0
74	1494.0
75	1079.0
76	774.0
77	528.5
78	357.0
79	255.0
80	175.5
81	125.0
82	86.0
83	53.5
84	38.5
85	28.0
86	20.0
87	16.5
88	13.5
89	11.0
90	9.0
91	7.0
92	4.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	5.78855095577379E-4
3	0.0015311650915272605
4	5.041641155028785E-4
5	1.8672745018625128E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	7.537823045491102
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.008592322591653698
26-27	0.023740678910541085
28-29	0.008394996357805589
30-31	1.0293060269049367E-4
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.027221108244161837
50-51	0.07082426642381863
52-53	0.05908645913620398
54-55	0.040812604974496715
56-57	0.005852278021056555
58-59	0.010229317105807318
60-61	0.037690161769625655
62-63	0.038258644372964036
64-65	0.0
66-67	0.0035912125714325505
68-69	0.03421202245301602
70-71	0.08708338903985581
72-73	0.07795679831611695
74-75	0.03998759518575842
76-77	0.009596023784620574
78-79	0.0012136803581467882
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.001735866227816394
92-93	0.003708896568949605
94-95	0.0012481720183447553
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	124.0
20-21	407.0
22-23	806.0
24-25	1889.0
26-27	2752.0
28-29	4501.0
30-31	6569.0
32-33	7937.0
34-35	10403.0
36-37	11307.0
38-39	12525.0
40-41	14401.0
42-43	17164.0
44-45	22074.0
46-47	21712.0
48-49	15800.0
50-51	14910.0
52-53	14201.0
54-55	18155.0
56-57	21908.0
58-59	31932.0
60-61	16134.0
62-63	20276.0
64-65	27802.0
66-67	37510.0
68-69	27214.0
70-71	32454.0
72-73	28565.0
74-75	26647.0
76-77	18528.0
78-79	25530.0
80-81	33542.0
82-83	43170.0
84-85	53098.0
86-87	56026.0
88-89	61597.0
90-91	63667.0
92-93	66198.0
94-95	82453.0
96-97	206619.0
98-99	37059.0
100-101	4139833.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.57999879268853
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.58191019466263	81.12325494475326
2	6.297732595895107	11.157062914821275
3	1.315885771182556	3.496834800680005
4	0.41125700006255583	1.457165782761036
5	0.16419603498851174	0.7272242290523306
6	0.08417530332448574	0.44737489601142827
7	0.042956293822457296	0.2663547918452149
8	0.028864548042189332	0.2045457304582905
9	0.017695472826772002	0.14107184654683647
>10	0.053511959265046594	0.7274886449882048
>50	0.0010322308581036373	0.06301435173761119
>100	7.233477487284116E-4	0.14971844091688088
>500	5.924732089363649E-5	0.03888862542763121
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	1.867274501862513E-5	0.0	0.0	0.0
5	0.0	1.867274501862513E-5	0.0	0.0	0.0
6	9.336372509312565E-5	3.734549003725026E-5	0.0	0.0	0.0
7	9.336372509312565E-5	3.734549003725026E-5	0.0	0.0	0.0
8	9.336372509312565E-5	7.469098007450052E-5	0.0	0.0	0.0
9	9.336372509312565E-5	1.6805470516762617E-4	0.0	1.867274501862513E-5	0.0
10-11	9.336372509312565E-5	1.6805470516762617E-4	0.0	7.469098007450052E-5	0.0
12-13	9.336372509312565E-5	1.867274501862513E-4	0.0	9.336372509312565E-5	0.0
14-15	9.336372509312565E-5	1.867274501862513E-4	0.0	1.2137284262106335E-4	0.0
16-17	1.4938196014900105E-4	1.867274501862513E-4	0.0	1.4938196014900105E-4	0.0
18-19	1.4938196014900105E-4	2.054001952048764E-4	0.0	1.4938196014900105E-4	9.336372509312566E-6
20-21	1.4938196014900105E-4	2.2407294022350156E-4	0.0	2.2407294022350156E-4	4.668186254656283E-5
22-23	1.4938196014900105E-4	2.987639202980021E-4	0.0	2.8009117527937695E-4	5.601823505587539E-5
24-25	1.4938196014900105E-4	3.0810029280731464E-4	0.0	3.454457828445649E-4	5.601823505587539E-5
26-27	1.4938196014900105E-4	3.174366653166272E-4	0.0	5.135004880121911E-4	5.601823505587539E-5
28-29	1.4938196014900105E-4	3.174366653166272E-4	0.0	0.0014191286214155097	5.601823505587539E-5
30-31	1.4938196014900105E-4	3.734549003725026E-4	0.0	0.004593495274581782	5.601823505587539E-5
32-33	1.4938196014900105E-4	3.734549003725026E-4	0.0	0.010148636917622758	5.601823505587539E-5
34-35	1.4938196014900105E-4	4.5748225295631567E-4	0.0	0.019064872664016255	5.601823505587539E-5
36-37	1.4938196014900105E-4	5.601823505587539E-4	0.0	0.029605637227030142	5.601823505587539E-5
38-39	1.4938196014900105E-4	5.975278405960042E-4	0.0	0.04596296186334575	5.601823505587539E-5
40-41	1.4938196014900105E-4	6.162005856146293E-4	0.0	0.0583336554381849	5.601823505587539E-5
42-43	1.4938196014900105E-4	6.722188206705047E-4	0.0	0.06563469874046733	7.469098007450051E-5
44-45	1.4938196014900105E-4	8.029280358008806E-4	0.0	0.07187139557668812	9.336372509312565E-5
46-47	1.4938196014900105E-4	8.77619015875381E-4	0.0	0.07833216535313242	9.336372509312565E-5
48-49	1.4938196014900105E-4	8.869553883846936E-4	0.0	0.08296300611775145	9.336372509312565E-5
50-51	2.1473656771418899E-4	0.0010550100935523199	0.0	0.0875378286473146	9.336372509312565E-5
52-53	2.2407294022350156E-4	0.0010830192110802576	0.0	0.10567840043290892	9.336372509312565E-5
54-55	2.2407294022350156E-4	0.00109235558358957	0.0	0.12389366319857772	9.336372509312565E-5
56-57	2.2407294022350156E-4	0.0011016919560988827	0.0	0.14531130173494075	9.336372509312565E-5
58-59	2.2407294022350156E-4	0.0011016919560988827	0.0	0.16271430009229937	9.336372509312565E-5
60-61	2.334093127328141E-4	0.0011297010736268202	0.0	0.1767188588562682	9.336372509312565E-5
62-63	2.4274568524212668E-4	0.0011390374461361329	0.0	0.18376782010079923	9.336372509312565E-5
64-65	2.4274568524212668E-4	0.0012510739162478837	0.0	0.18557907636760584	9.336372509312565E-5
66-67	2.5208205775143925E-4	0.0012510739162478837	0.0	0.1884173336104369	1.307092151303759E-4
68-69	2.614184302607518E-4	0.0012510739162478837	0.0	0.1914890001660007	1.307092151303759E-4
70-71	2.614184302607518E-4	0.001260410288757196	0.0	0.1935710112355774	1.307092151303759E-4
72-73	2.8009117527937695E-4	0.0012697466612665088	0.0	0.19595178622545212	1.307092151303759E-4
74-75	2.8009117527937695E-4	0.0013070921513037592	0.0	0.19739892396439557	1.307092151303759E-4
76-77	2.8009117527937695E-4	0.0013631103863596344	0.0	0.19837924307787339	1.307092151303759E-4
78-79	2.8009117527937695E-4	0.001391119503887572	0.0	0.19893008905592283	1.307092151303759E-4
80-81	2.8009117527937695E-4	0.0014004558763968846	0.0	0.19919150748618358	1.307092151303759E-4
82-83	2.8009117527937695E-4	0.0014004558763968846	0.0	0.19989173542438202	1.307092151303759E-4
84-85	2.894275477886895E-4	0.0014004558763968846	0.0	0.20051727238250594	1.307092151303759E-4
86-87	3.0810029280731464E-4	0.0014004558763968846	0.0	0.20094674551793434	1.307092151303759E-4
88	3.3610941033525234E-4	0.001437801366434135	0.0	0.20097475463546227	1.307092151303759E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6325	0.0	52.518185	1
GTATCAA	12115	0.0	39.19027	1
TCAACGC	14115	0.0	32.920403	4
ATCAACG	14210	0.0	32.825054	3
CAACGCA	14530	0.0	31.919744	5
AACGCAG	14880	0.0	31.257412	6
TATCAAC	15075	0.0	31.087683	2
CGCAGAG	17230	0.0	26.919323	8
ACGCAGA	17510	0.0	26.53749	7
GTACATG	15455	0.0	26.348349	1
TACATGG	15065	0.0	26.302258	2
ACATGGG	15655	0.0	24.693869	3
GAGTACT	12150	0.0	22.53888	12-13
GCAGAGT	19950	0.0	22.00936	9
CATGGGG	10660	0.0	21.527546	4
GTGGTAT	3140	0.0	20.82241	1
CAGAGTA	18710	0.0	20.372126	10-11
AGAGTAC	16995	0.0	20.358583	10-11
GTACTTT	13840	0.0	19.619148	14-15
TGGTATC	3240	0.0	18.960833	2
>>END_MODULE