FastQCFastQC Report
Fri 27 May 2016
SRR2139755_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139755_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5277348
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57450.041.2061421
GTATCAA96100.035.3655741
TCAACGC102900.031.548344
ATCAACG103150.031.4296913
CAACGCA107150.030.2563935
AACGCAG110900.029.153996
TATCAAC117600.027.9378322
GTACATG129500.026.64751
ACGCAGA127250.025.4422727
GTCAACG6750.025.1428151
TACATGG131050.025.0040872
ACATGGG126450.024.9500983
GCGCAGA13150.024.4883231
CGCAGAG134200.024.0598058
CATGGGG97000.023.5526964
GCAGAGT163550.019.7155279
CTTATAC47600.019.2898371
GAGTACT96050.018.66553912-13
TATGCCG26400.018.43878742-43
CTCGTAT26150.018.10306538-39
Produced by FastQC (version 0.11.5)