##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139755_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5277348
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.62940609563743	18.0	18.0	32.0	18.0	33.0
2	28.09507142602686	28.0	27.0	32.0	18.0	33.0
3	31.195348307521126	32.0	31.0	33.0	27.0	33.0
4	31.548738874146636	33.0	32.0	33.0	30.0	33.0
5	32.450321638823134	33.0	33.0	33.0	32.0	33.0
6	36.42188216505715	38.0	37.0	38.0	34.0	38.0
7	36.97365551788512	38.0	38.0	38.0	35.0	38.0
8	36.93331972801491	38.0	38.0	38.0	35.0	38.0
9	37.182550970676935	38.0	38.0	38.0	36.0	38.0
10-11	37.29504194152062	38.0	38.0	38.0	36.5	38.0
12-13	37.32327553536359	38.0	38.0	38.0	37.0	38.0
14-15	37.31615093414344	38.0	38.0	38.0	37.0	38.0
16-17	37.265032834673775	38.0	38.0	38.0	36.5	38.0
18-19	37.25569708497525	38.0	38.0	38.0	36.5	38.0
20-21	37.32377472115888	38.0	38.0	38.0	37.0	38.0
22-23	37.34441448275972	38.0	38.0	38.0	37.0	38.0
24-25	37.3489575675525	38.0	38.0	38.0	37.0	38.0
26-27	37.25740400912239	38.0	38.0	38.0	37.0	38.0
28-29	37.16889392833526	38.0	38.0	38.0	36.0	38.0
30-31	37.25070503553603	38.0	38.0	38.0	37.0	38.0
32-33	37.274771187041914	38.0	38.0	38.0	37.0	38.0
34-35	37.22731265259391	38.0	38.0	38.0	37.0	38.0
36-37	37.23448480031472	38.0	38.0	38.0	37.0	38.0
38-39	37.22656781095171	38.0	38.0	38.0	37.0	38.0
40-41	37.197093383730326	38.0	38.0	38.0	36.5	38.0
42-43	37.19750231966741	38.0	38.0	38.0	36.0	38.0
44-45	37.13625182395471	38.0	38.0	38.0	36.0	38.0
46-47	37.11496740090853	38.0	38.0	38.0	36.0	38.0
48-49	37.090054637572024	38.0	38.0	38.0	36.0	38.0
50-51	37.084397855517935	38.0	38.0	38.0	36.0	38.0
52-53	37.06105615630135	38.0	38.0	38.0	36.0	38.0
54-55	37.00898495865762	38.0	38.0	38.0	36.0	38.0
56-57	37.003478136345564	38.0	38.0	38.0	36.0	38.0
58-59	37.013163770354	38.0	38.0	38.0	36.0	38.0
60-61	37.01986438088251	38.0	38.0	38.0	36.0	38.0
62-63	36.94515439511063	38.0	38.0	38.0	36.0	38.0
64-65	36.972823387588605	38.0	38.0	38.0	36.0	38.0
66-67	36.989564024024176	38.0	38.0	38.0	36.0	38.0
68-69	36.95414759555619	38.0	38.0	38.0	36.0	38.0
70-71	36.902098197305435	38.0	38.0	38.0	35.5	38.0
72-73	36.91226641277747	38.0	38.0	38.0	36.0	38.0
74-75	36.93067159109019	38.0	38.0	38.0	36.0	38.0
76-77	36.86379867926495	38.0	38.0	38.0	35.0	38.0
78-79	36.88770861801647	38.0	38.0	38.0	35.0	38.0
80-81	36.86064526458959	38.0	38.0	38.0	35.0	38.0
82-83	36.80818658028095	38.0	38.0	38.0	35.0	38.0
84-85	36.83670879439352	38.0	38.0	38.0	35.0	38.0
86-87	36.85669412655217	38.0	38.0	38.0	35.0	38.0
88-89	36.76535343508424	38.0	38.0	38.0	35.0	38.0
90-91	36.72061647197991	38.0	38.0	38.0	35.0	38.0
92-93	36.7723749501483	38.0	38.0	38.0	35.0	38.0
94-95	36.753645013470646	38.0	38.0	38.0	35.0	38.0
96-97	36.700380564703536	38.0	38.0	38.0	34.0	38.0
98-99	36.71642232193088	38.0	38.0	38.0	34.5	38.0
100	35.08070402484793	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	5.0
19	19.0
20	89.0
21	256.0
22	624.0
23	1518.0
24	3132.0
25	5950.0
26	10035.0
27	16065.0
28	23527.0
29	33943.0
30	46493.0
31	61838.0
32	81930.0
33	112145.0
34	169273.0
35	318670.0
36	991385.0
37	3400451.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.863880115542884	14.352151876283315	12.675836423900794	24.10813158427301
2	14.964886712963917	20.473052084693315	41.552185217307105	23.009875985035663
3	18.345938149237078	25.754450909813038	30.175762523146094	25.72384841780379
4	12.19705890043973	17.662552351124976	38.78597310087305	31.354415647562238
5	13.338008755330911	37.48266613507586	35.9707589684157	13.208566141177533
6	30.459838069814012	37.70207165130285	18.66358329278257	13.174506986100562
7	26.952449204581242	32.38409988657177	22.969538097369398	17.69391281147759
8	24.779946291205356	36.47866314671687	21.27976021289481	17.46163034918296
9	25.702853023905188	16.408677237127435	21.08443483355655	36.80403490541082
10-11	24.245075367400446	26.6246322963731	29.14077297915544	19.989519357071014
12-13	24.903578464031558	24.02162980345431	29.26733275880234	21.807458973711796
14-15	21.93144170140002	25.5384806914382	26.578520120333167	25.951557486828612
16-17	21.853400609548583	28.787754758640133	27.314543213750543	22.044301418060737
18-19	21.873808587191903	28.05509509700706	28.802459113933743	21.268637201867303
20-21	22.475724158487868	27.287318311953197	28.73282603890331	21.504131490655624
22-23	22.16145905384053	27.287342281172055	28.640869334847334	21.910329330140076
24-25	22.056288209764226	27.35700197986252	28.725328235128906	21.861381575244344
26-27	22.03310869738474	27.567098195252175	28.63073976152951	21.76905334583357
28-29	21.94547765589906	27.499432149765333	28.697662504706805	21.857427689628796
30-31	22.266721705834627	27.404030195626284	28.60945440052382	21.71979369801527
32-33	21.957320832440082	27.580878462816038	28.649697230127448	21.812103474616436
34-35	22.106473561916186	27.464859737236395	28.63728189040671	21.791384810440707
36-37	22.015364590282534	27.53797844335798	28.6305302965308	21.816126669828687
38-39	21.87196423934635	27.51502660966635	28.794409408071314	21.818599742915985
40-41	22.118431638161223	27.356507270463243	28.723490827498676	21.801570263876858
42-43	22.064272213189568	27.41053833910173	28.602061777140662	21.923127670568036
44-45	22.009873989192705	27.56910761185899	28.529166214171553	21.89185218477675
46-47	22.13861926437359	27.470038573159272	28.320295812861318	22.071046349605822
48-49	22.021435867091082	27.575770641078822	28.33516946149657	22.06762403033353
50-51	21.961701673897142	27.6910689342538	28.36432058521312	21.982908806635937
52-53	22.1027600987278	27.631304252913797	28.345318169227905	21.920617479130495
54-55	22.12640434049758	27.512972087274445	28.25332765528646	22.107295916941514
56-57	22.099827544050058	27.717612339202415	28.181916819819307	22.00064329692822
58-59	21.995897243340988	27.7668658528501	28.33657967214106	21.900657231667857
60-61	22.107722977965892	27.85531912093859	28.181658929666288	21.855298971429228
62-63	22.00424308713661	27.96739862299345	28.155964179786388	21.87239411008355
64-65	21.993808641491892	27.96781559974073	28.114185013438288	21.924190745329092
66-67	22.01074725037955	28.005601149601446	28.017244860848734	21.966406739170274
68-69	21.994973081405952	27.94798645744959	28.081984760666344	21.975055700478112
70-71	22.040884701	27.710988068431092	28.14626703027473	22.10186020029418
72-73	22.125086511883833	27.62874687804941	28.116620168768026	22.129546441298732
74-75	21.99525062970711	27.75918632188282	28.07961298605619	22.165950062353883
76-77	22.16921735919678	27.694879876996552	27.966576420837786	22.16932634296888
78-79	22.1177773424942	27.69773098186507	27.936242716075842	22.248248959564886
80-81	22.118020781379517	27.756836440125525	27.913754486928994	22.211388291565967
82-83	22.187686612959663	27.730419663946464	27.96104638920191	22.120847333891962
84-85	22.228102039798916	27.700587861990538	27.932191293857787	22.139118804352755
86-87	22.06139421713658	27.758556511221173	28.03729723744233	22.142752034199916
88-89	22.16488253158578	27.758555208490538	27.939377553500115	22.13718470642357
90-91	22.25648992805276	27.800261530211472	27.89364054973292	22.049607992002848
92-93	22.22660531470308	27.759476692751218	27.9618728124937	22.052045180052005
94-95	22.18807350548292	27.844870642351854	27.923487757248676	22.043568094916548
96-97	22.263883202612465	27.890045961430488	28.04375603029861	21.802314805658433
98-99	23.261063342976623	29.811035845743266	28.292718678978517	18.635182132301594
100	22.166483326862515	30.73964130106553	22.319275484232776	24.77459988783918
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	630.0
1	575.5
2	934.5
3	1484.0
4	1821.0
5	2460.0
6	3062.0
7	3857.5
8	4996.5
9	5708.0
10	5631.5
11	5381.0
12	5879.0
13	6958.5
14	8792.0
15	10934.5
16	12681.0
17	14041.5
18	14432.0
19	13850.5
20	12989.5
21	12835.5
22	14242.0
23	17480.0
24	22503.5
25	29181.0
26	38037.0
27	48710.0
28	59318.5
29	71171.5
30	84122.0
31	96597.5
32	109606.0
33	122367.5
34	134650.5
35	145636.5
36	157592.5
37	169439.0
38	178989.5
39	187857.0
40	196332.5
41	203554.5
42	208767.5
43	216477.5
44	221858.5
45	223768.0
46	224506.0
47	221142.0
48	217606.0
49	213176.5
50	207635.0
51	201124.0
52	192570.5
53	183353.0
54	172188.0
55	159591.0
56	146897.5
57	133299.0
58	119048.5
59	104189.5
60	87973.5
61	71719.0
62	57093.0
63	44005.0
64	32975.5
65	24639.0
66	18387.0
67	13970.0
68	10546.5
69	7769.0
70	5724.5
71	4112.0
72	2879.5
73	1949.5
74	1280.0
75	836.0
76	538.5
77	331.0
78	221.5
79	160.5
80	109.5
81	66.5
82	40.0
83	27.5
84	18.5
85	10.5
86	6.5
87	4.0
88	2.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	1.894891146083222E-5
3	0.0
4	3.789782292166444E-5
5	0.0020085846148482156
6	2.2738693752998666E-4
7	3.789782292166444E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	9.574678558607034E-4
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	1.9432025221214175E-5
44-45	1.9549740159516104E-5
46-47	2.9529305818188652E-5
48-49	1.4867412416073457E-4
50-51	5.586043270887687E-4
52-53	2.3073752943909913E-4
54-55	3.531111208412842E-4
56-57	5.277889510022052E-4
58-59	4.3922163389222067E-4
60-61	1.1308385321921459E-4
62-63	4.4487451279774654E-4
64-65	9.372632433994538E-5
66-67	1.468574036639034E-4
68-69	6.237357457881452E-4
70-71	1.2787575250884233E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	9.11854241923137E-4
90-91	0.0
92-93	1.158866855353368E-5
94-95	4.675413508181331E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	90.0
20-21	233.0
22-23	327.0
24-25	373.0
26-27	1247.0
28-29	4030.0
30-31	9003.0
32-33	11759.0
34-35	16664.0
36-37	25631.0
38-39	27895.0
40-41	26912.0
42-43	29683.0
44-45	34762.0
46-47	35676.0
48-49	32950.0
50-51	29324.0
52-53	27526.0
54-55	29932.0
56-57	30455.0
58-59	31247.0
60-61	31181.0
62-63	31102.0
64-65	33785.0
66-67	36407.0
68-69	37321.0
70-71	40913.0
72-73	32681.0
74-75	32195.0
76-77	33431.0
78-79	34729.0
80-81	34238.0
82-83	32543.0
84-85	32221.0
86-87	33432.0
88-89	35641.0
90-91	36280.0
92-93	35904.0
94-95	43743.0
96-97	161110.0
98-99	373812.0
100-101	3708960.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.24162866174598
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.13825636103384	82.22568059712137
2	5.858346188681013	10.456167102844516
3	1.2001680128917156	3.2131484441456415
4	0.4020908109291024	1.4353295534894108
5	0.17594512064823745	0.7850814560868051
6	0.0796048966335696	0.42624423750178136
7	0.04796507211498722	0.2996336808093689
8	0.02666219599548434	0.19035022354685632
9	0.01714427979906646	0.13769851063512256
>10	0.052591802712063596	0.6888523290912305
>50	7.499045303951561E-4	0.043560942625095016
>100	4.174835849634805E-4	0.05944206992628592
>500	5.7870445538865634E-5	0.03881085217652153
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.894891146083222E-5	0.0	0.0	0.0
3	0.0	1.894891146083222E-5	0.0	0.0	0.0
4	0.0	7.579564584332888E-5	0.0	0.0	0.0
5	0.0	7.579564584332888E-5	0.0	0.0	0.0
6	1.894891146083222E-5	7.579564584332888E-5	0.0	0.0	1.894891146083222E-5
7	1.894891146083222E-5	7.579564584332888E-5	0.0	0.0	1.894891146083222E-5
8	1.894891146083222E-5	7.579564584332888E-5	0.0	0.0	3.789782292166444E-5
9	1.894891146083222E-5	1.3264238022582555E-4	0.0	1.894891146083222E-5	3.789782292166444E-5
10-11	5.6846734382496666E-5	1.3264238022582555E-4	0.0	1.894891146083222E-5	3.789782292166444E-5
12-13	9.474455730416111E-5	1.3264238022582555E-4	0.0	1.894891146083222E-5	3.789782292166444E-5
14-15	9.474455730416111E-5	1.5159129168665776E-4	0.0	3.789782292166444E-5	3.789782292166444E-5
16-17	9.474455730416111E-5	1.5159129168665776E-4	0.0	7.579564584332888E-5	3.789782292166444E-5
18-19	9.474455730416111E-5	1.7054020314749E-4	0.0	1.1369346876499333E-4	3.789782292166444E-5
20-21	9.474455730416111E-5	1.8948911460832222E-4	0.0	1.7054020314749E-4	3.789782292166444E-5
22-23	9.474455730416111E-5	2.4633584899081887E-4	0.0	2.842336719124833E-4	6.632119011291278E-5
24-25	1.1369346876499333E-4	2.842336719124833E-4	0.0	3.6950377348622833E-4	9.474455730416111E-5
26-27	1.3264238022582555E-4	3.031825833733155E-4	0.0	4.3582496359914104E-4	9.474455730416111E-5
28-29	1.3264238022582555E-4	3.031825833733155E-4	0.0	9.569200287720272E-4	9.474455730416111E-5
30-31	1.3264238022582555E-4	3.2213149483414775E-4	0.0	0.0020654313492307124	9.474455730416111E-5
32-33	1.5159129168665776E-4	3.2213149483414775E-4	0.0	0.004149811609922257	9.474455730416111E-5
34-35	1.7054020314749E-4	3.979271406774766E-4	0.0	0.006764761391517103	9.474455730416111E-5
36-37	1.7054020314749E-4	4.926716979816377E-4	0.0	0.011104062116047681	9.474455730416111E-5
38-39	1.7054020314749E-4	5.495184323641344E-4	0.0	0.02319346762805864	9.474455730416111E-5
40-41	1.8948911460832222E-4	6.347885339378794E-4	0.0	0.03615452306726788	1.0421901303457723E-4
42-43	1.8948911460832222E-4	7.011097240507922E-4	0.0	0.0436961898286791	1.1369346876499333E-4
44-45	1.9896357033873834E-4	7.863798256245372E-4	0.0	0.05249795920223567	1.1369346876499333E-4
46-47	2.1791248179957055E-4	8.62175471467866E-4	0.0	0.06252193336501591	1.2316792449540943E-4
48-49	2.2738693752998666E-4	8.716499271982822E-4	0.0	0.07251748416060491	1.3264238022582555E-4
50-51	2.4633584899081887E-4	9.37971117311195E-4	0.0	0.08256040723484598	1.3264238022582555E-4
52-53	2.4633584899081887E-4	9.47445573041611E-4	0.0	0.10836882464449947	1.4211683595624164E-4
54-55	2.4633584899081887E-4	9.47445573041611E-4	0.0	0.14117886483893044	1.5159129168665776E-4
56-57	2.4633584899081887E-4	9.47445573041611E-4	0.0	0.19141242912159667	1.6106574741707387E-4
58-59	2.4633584899081887E-4	9.569200287720271E-4	0.0	0.22896917163696615	1.800146588779061E-4
60-61	2.8423367191248334E-4	9.663944845024432E-4	0.0	0.2443935855660836	1.8948911460832222E-4
62-63	3.031825833733155E-4	9.663944845024432E-4	0.0	0.2554029031248271	1.8948911460832222E-4
64-65	3.031825833733155E-4	0.0010706134975370205	0.0	0.25943902126598434	1.8948911460832222E-4
66-67	3.031825833733155E-4	0.0010895624089978526	0.0	0.26355093505298494	1.8948911460832222E-4
68-69	3.031825833733155E-4	0.001117985776189101	0.0	0.26784286349886344	1.8948911460832222E-4
70-71	3.031825833733155E-4	0.0011369346876499333	0.0	0.27172739034833404	1.8948911460832222E-4
72-73	3.6950377348622833E-4	0.0011369346876499333	0.0	0.27512871995555344	2.2738693752998666E-4
74-75	3.7897822921664445E-4	0.0011369346876499333	0.0	0.27771524636995704	2.2738693752998666E-4
76-77	3.7897822921664445E-4	0.0011558835991107655	0.0	0.2801691304041348	2.2738693752998666E-4
78-79	3.7897822921664445E-4	0.0011558835991107655	0.0	0.2814387074720106	2.2738693752998666E-4
80-81	3.7897822921664445E-4	0.0011558835991107655	0.0	0.28183663461268804	2.3686139326040275E-4
82-83	3.884526849470605E-4	0.0011558835991107655	0.0	0.2821113938288701	2.4633584899081887E-4
84-85	3.979271406774766E-4	0.001203255877762846	0.0	0.2822724595762872	2.4633584899081887E-4
86-87	3.979271406774766E-4	0.001212730333493262	0.0	0.28231035739920884	2.4633584899081887E-4
88	3.979271406774766E-4	0.0012506281564149265	0.0	0.28231983185493925	2.652847604516511E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5745	0.0	41.206142	1
GTATCAA	9610	0.0	35.365574	1
TCAACGC	10290	0.0	31.54834	4
ATCAACG	10315	0.0	31.429691	3
CAACGCA	10715	0.0	30.256393	5
AACGCAG	11090	0.0	29.15399	6
TATCAAC	11760	0.0	27.937832	2
GTACATG	12950	0.0	26.6475	1
ACGCAGA	12725	0.0	25.442272	7
GTCAACG	675	0.0	25.142815	1
TACATGG	13105	0.0	25.004087	2
ACATGGG	12645	0.0	24.950098	3
GCGCAGA	1315	0.0	24.488323	1
CGCAGAG	13420	0.0	24.059805	8
CATGGGG	9700	0.0	23.552696	4
GCAGAGT	16355	0.0	19.715527	9
CTTATAC	4760	0.0	19.289837	1
GAGTACT	9605	0.0	18.665539	12-13
TATGCCG	2640	0.0	18.438787	42-43
CTCGTAT	2615	0.0	18.103065	38-39
>>END_MODULE