FastQCFastQC Report
Fri 27 May 2016
SRR2139754_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139754_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5176505
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53750.047.200031
GTATCAA95400.036.7068941
ATCAACG107350.031.9787673
TCAACGC108200.031.7275494
CAACGCA111600.030.7609395
AACGCAG114850.029.9273976
TATCAAC118100.029.323222
ACGCAGA133100.025.8560147
GTACATG141050.025.1933731
TACATGG138600.025.0793232
CGCAGAG137400.025.015498
ACATGGG140850.024.0059173
GTCAACG7950.023.8391631
CTTATAC69750.022.4782581
CATGGGG101700.022.2353424
TATACAC86100.021.0612643
GCAGAGT171900.020.07019
GCGCAGA11600.019.6799221
GAGTACT97100.019.16273112-13
TTATACA90950.018.422662
Produced by FastQC (version 0.11.5)