##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139754_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5176505
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.725880106365203	25.0	18.0	32.0	18.0	33.0
2	28.291467312404798	30.0	27.0	32.0	18.0	33.0
3	31.257432959110442	32.0	31.0	33.0	27.0	33.0
4	31.58303797639527	33.0	32.0	33.0	30.0	33.0
5	32.45475161329893	33.0	33.0	33.0	32.0	33.0
6	36.4236244338603	38.0	37.0	38.0	34.0	38.0
7	36.994376514656125	38.0	38.0	38.0	35.0	38.0
8	36.956254268082425	38.0	38.0	38.0	35.0	38.0
9	37.19636839914189	38.0	38.0	38.0	36.0	38.0
10-11	37.310911899051575	38.0	38.0	38.0	36.5	38.0
12-13	37.33625969645543	38.0	38.0	38.0	37.0	38.0
14-15	37.33139492765872	38.0	38.0	38.0	37.0	38.0
16-17	37.27948200571621	38.0	38.0	38.0	37.0	38.0
18-19	37.270592030723435	38.0	38.0	38.0	36.5	38.0
20-21	37.335495622818534	38.0	38.0	38.0	37.0	38.0
22-23	37.360211722358414	38.0	38.0	38.0	37.0	38.0
24-25	37.36249967592215	38.0	38.0	38.0	37.0	38.0
26-27	37.27061075841661	38.0	38.0	38.0	37.0	38.0
28-29	37.18113761207519	38.0	38.0	38.0	36.0	38.0
30-31	37.25829760388956	38.0	38.0	38.0	37.0	38.0
32-33	37.28089865833961	38.0	38.0	38.0	37.0	38.0
34-35	37.23350632763224	38.0	38.0	38.0	37.0	38.0
36-37	37.23825012558352	38.0	38.0	38.0	37.0	38.0
38-39	37.21789117520866	38.0	38.0	38.0	37.0	38.0
40-41	37.2033922223314	38.0	38.0	38.0	37.0	38.0
42-43	37.207684996513976	38.0	38.0	38.0	37.0	38.0
44-45	37.148043660187795	38.0	38.0	38.0	36.0	38.0
46-47	37.1264113549466	38.0	38.0	38.0	36.0	38.0
48-49	37.1007271037327	38.0	38.0	38.0	36.0	38.0
50-51	37.09285217704212	38.0	38.0	38.0	36.0	38.0
52-53	37.07247838854631	38.0	38.0	38.0	36.0	38.0
54-55	37.02391207189715	38.0	38.0	38.0	36.0	38.0
56-57	37.01611359431974	38.0	38.0	38.0	36.0	38.0
58-59	37.02406284656499	38.0	38.0	38.0	36.0	38.0
60-61	37.02923104792997	38.0	38.0	38.0	36.0	38.0
62-63	36.957783443701835	38.0	38.0	38.0	36.0	38.0
64-65	36.985123074243624	38.0	38.0	38.0	36.0	38.0
66-67	36.996149946761975	38.0	38.0	38.0	36.0	38.0
68-69	36.96607220401805	38.0	38.0	38.0	36.0	38.0
70-71	36.91405414525492	38.0	38.0	38.0	36.0	38.0
72-73	36.929766281006025	38.0	38.0	38.0	36.0	38.0
74-75	36.94948478434746	38.0	38.0	38.0	36.0	38.0
76-77	36.88332351360785	38.0	38.0	38.0	35.5	38.0
78-79	36.90676530145941	38.0	38.0	38.0	35.0	38.0
80-81	36.876873620911674	38.0	38.0	38.0	35.0	38.0
82-83	36.82768228448906	38.0	38.0	38.0	35.0	38.0
84-85	36.85291278284595	38.0	38.0	38.0	35.0	38.0
86-87	36.8717184713727	38.0	38.0	38.0	35.0	38.0
88-89	36.779770502090344	38.0	38.0	38.0	35.0	38.0
90-91	36.73301334684563	38.0	38.0	38.0	35.0	38.0
92-93	36.78409670210135	38.0	38.0	38.0	35.0	38.0
94-95	36.7643797592588	38.0	38.0	38.0	35.0	38.0
96-97	36.714582304753776	38.0	38.0	38.0	34.5	38.0
98-99	36.725087724147	38.0	38.0	38.0	34.5	38.0
100	35.093272684138356	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	6.0
19	26.0
20	85.0
21	253.0
22	567.0
23	1341.0
24	2961.0
25	5571.0
26	9622.0
27	15351.0
28	22840.0
29	32761.0
30	44910.0
31	59486.0
32	79275.0
33	108979.0
34	164104.0
35	311141.0
36	981924.0
37	3335302.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.9488187493299	15.159282179771875	12.690241775097292	24.20165729580093
2	14.90882361747936	20.507504580793412	41.628724399957115	22.954947401770113
3	18.239082160646998	26.029724688762013	30.2583306690518	25.472862481539188
4	12.175581956920698	17.77981261470105	38.57737853762195	31.4672268907563
5	13.22567583439971	37.55615854676286	35.96061444829782	13.257551170539617
6	30.278916633326848	37.698534509754	18.669473715119484	13.35307514179967
7	26.767935805311037	32.428842405770844	22.90642930178926	17.89679248712886
8	24.805056693657207	36.41878062515153	21.32942979867691	17.44673288251436
9	25.648869266039537	16.38557289136203	20.963642457604116	37.00191538499432
10-11	24.187342618233732	26.59516411169312	29.133778485677116	20.083714784396037
12-13	24.906341247617842	24.009413687420373	29.208925713391565	21.87531935157022
14-15	21.95907277207305	25.477759608075335	26.455349700232105	26.107817919619514
16-17	21.891131178275693	28.8260612131158	27.14484966207895	22.13795794652956
18-19	21.9392041541542	27.891173677993166	28.712248901527186	21.457373266325448
20-21	22.50658057354609	27.152077680083526	28.585368181290878	21.755973565079508
22-23	22.251353278823206	27.295464097432404	28.47222913148377	21.980953492260625
24-25	22.159632259145827	27.284906907988955	28.64258628042993	21.912874552435287
26-27	22.17773660910266	27.447127364236117	28.557899052759794	21.81723697390143
28-29	21.964269767431073	27.309742426403815	28.578561164595897	22.14742664156922
30-31	22.33023855368694	27.302704048356468	28.569774091954418	21.79728330600217
32-33	22.228082215752316	27.31550884882183	28.665030888252108	21.791378047173744
34-35	22.023184059085587	27.3507658528244	28.768313686970775	21.857736401119237
36-37	22.1560135351258	27.386950982264896	28.573241294061415	21.88379418854789
38-39	21.85819482291704	27.37689705450565	28.83164541019296	21.933262712384355
40-41	22.13228906895929	27.220549726069226	28.738091447726806	21.90906975724468
42-43	22.081107757051267	27.2829979301085	28.66474364291963	21.971150669920608
44-45	22.005526977475956	27.487559999295776	28.560965206410383	21.94594781681789
46-47	22.247209746102094	27.302697271477317	28.266242537388287	22.183850445032302
48-49	22.07401791632411	27.403144927318312	28.26969054250057	22.25314661385701
50-51	21.908894841467813	27.591369954485796	28.395131847917575	22.10460335612881
52-53	22.136441422290773	27.50805591808217	28.43017119495363	21.925331464673427
54-55	22.07196693310338	27.466263556764286	28.243808248482637	22.217961261649695
56-57	22.195618109598602	27.659363098587313	28.090015794807748	22.05500299700633
58-59	21.911868554118385	27.79008237927203	28.298240036263252	21.999809030346338
60-61	22.243053699161976	27.95182233443663	28.066528549225474	21.73859541717592
62-63	21.864228160156195	28.117778673418712	28.037351771720743	21.98064139470435
64-65	21.87468756546726	28.10593278685977	28.013787036049788	22.005592611623182
66-67	21.925068725820317	28.119682091404936	28.063145072951777	21.89210410982297
68-69	21.936970542277738	28.1839143262316	27.97009648750904	21.909018643981625
70-71	21.982284695006467	27.874792540801273	27.890229058754734	22.252693705437522
72-73	22.129754602593398	27.579545910117464	27.92773048602852	22.36296900126062
74-75	21.965859571068123	27.748353469752267	28.114959993193196	22.17082696598641
76-77	22.150835804916586	27.513793363684407	27.987683363228435	22.347687468170573
78-79	22.11572458067766	27.514904389999582	27.76331184073994	22.606059188582815
80-81	22.29805509283492	27.75160115658616	27.740439863123424	22.2099038874555
82-83	22.32518967078945	27.540694044666136	27.80567798192206	22.328438302622352
84-85	22.202131486581376	27.53027488430122	27.771519645519426	22.496073983597974
86-87	22.116071618055898	27.701876755257814	27.856928950229083	22.325122676457205
88-89	22.375275993839427	27.702955952286956	27.775706073338974	22.146061980534647
90-91	22.404946057720885	27.760911587742456	27.745973781209965	22.08816857332669
92-93	22.20898746609813	27.52921011830532	27.807676704675664	22.454125710920884
94-95	22.34759653303992	27.79205815167553	27.80164990350209	22.05869541178246
96-97	22.61245258434083	27.658244457754684	27.891968003552627	21.83733495435186
98-99	23.35305771836812	29.717619843332077	28.21008289720835	18.719239541091454
100	22.270956493311903	30.59623051007528	22.220018207244756	24.91279478936806
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	587.0
1	544.0
2	895.0
3	1429.0
4	1826.0
5	2555.5
6	3206.0
7	3955.0
8	5112.0
9	5827.5
10	5732.0
11	5633.5
12	6039.0
13	7108.0
14	9093.5
15	11249.5
16	13118.0
17	14539.5
18	14754.0
19	14036.0
20	13327.5
21	13340.5
22	14694.5
23	17808.5
24	23106.0
25	30297.5
26	39475.0
27	50003.0
28	60934.5
29	73123.0
30	84740.5
31	96512.5
32	109473.5
33	121136.0
34	132363.0
35	142417.5
36	152417.5
37	162969.5
38	171743.0
39	178791.0
40	186481.0
41	194084.5
42	199350.0
43	203762.5
44	207892.5
45	213084.0
46	224545.5
47	229600.5
48	225809.0
49	221126.0
50	213661.0
51	204473.0
52	194292.0
53	184611.0
54	173774.0
55	161707.0
56	149015.5
57	135382.5
58	120954.0
59	105785.5
60	89262.5
61	73179.5
62	58133.0
63	44946.0
64	34140.5
65	25249.0
66	18525.5
67	14054.5
68	10685.0
69	7785.5
70	5602.0
71	4017.5
72	2815.0
73	1986.5
74	1369.5
75	900.5
76	603.5
77	393.5
78	238.0
79	150.0
80	98.5
81	60.0
82	40.0
83	27.5
84	19.0
85	14.5
86	12.0
87	8.0
88	4.5
89	2.5
90	1.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	9.659026698515696E-5
5	0.001719306752335794
6	2.3181664076437673E-4
7	3.863610679406279E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	9.278083890115013E-4
30-31	0.0
32-33	0.0
34-35	9.72134919105737E-6
36-37	0.0
38-39	0.0
40-41	0.0
42-43	9.934683430319024E-6
44-45	1.0003216033954916E-5
46-47	3.02430939894875E-5
48-49	8.127890735139269E-5
50-51	3.070088587406189E-4
52-53	1.8538258330629839E-4
54-55	2.9020512112539886E-4
56-57	3.4436235518389E-4
58-59	3.672479852513209E-4
60-61	2.110703663221189E-5
62-63	3.7219768376064283E-4
64-65	7.501102126219545E-5
66-67	1.7292883405651811E-4
68-69	5.018660689659987E-4
70-71	1.2120020819992128E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	9.037200446532831E-4
90-91	0.0
92-93	1.2106188707879845E-5
94-95	6.109392335400752E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	73.0
20-21	162.0
22-23	272.0
24-25	368.0
26-27	1343.0
28-29	4303.0
30-31	9647.0
32-33	13062.0
34-35	18201.0
36-37	28143.0
38-39	30595.0
40-41	29665.0
42-43	32981.0
44-45	37845.0
46-47	39163.0
48-49	36477.0
50-51	32017.0
52-53	30075.0
54-55	32375.0
56-57	33949.0
58-59	18897.0
60-61	36330.0
62-63	35588.0
64-65	38300.0
66-67	42300.0
68-69	44203.0
70-71	50688.0
72-73	36563.0
74-75	34929.0
76-77	36122.0
78-79	37323.0
80-81	36366.0
82-83	34382.0
84-85	34305.0
86-87	35464.0
88-89	37305.0
90-91	37364.0
92-93	37045.0
94-95	45196.0
96-97	173903.0
98-99	359344.0
100-101	3523872.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.18027621590763
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.25272086038775	80.4669013092211
2	6.440683637911325	11.35882524420595
3	1.4074681846278443	3.723327998567562
4	0.462823523617895	1.6324762460738254
5	0.18715839616054988	0.8251839534781779
6	0.09299150389938925	0.49200098997484765
7	0.05303600505806404	0.32737107027858525
8	0.028308111006871604	0.19969736381892106
9	0.019923730404248788	0.1581192045268142
>10	0.05379420382839296	0.6987375969357749
>50	8.135127105168829E-4	0.046203861269351665
>100	2.173504440740865E-4	0.03811209617534037
>500	6.097994303161898E-5	0.0330430654737081
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.9318053397031395E-5	0.0	0.0	0.0
3	0.0	1.9318053397031395E-5	0.0	0.0	0.0
4	0.0	3.863610679406279E-5	0.0	1.9318053397031395E-5	0.0
5	0.0	5.795416019109418E-5	0.0	1.9318053397031395E-5	0.0
6	0.0	5.795416019109418E-5	0.0	1.9318053397031395E-5	0.0
7	0.0	5.795416019109418E-5	0.0	1.9318053397031395E-5	0.0
8	0.0	1.1590832038218836E-4	0.0	1.9318053397031395E-5	0.0
9	0.0	2.1249858736734534E-4	0.0	1.9318053397031395E-5	0.0
10-11	0.0	2.1249858736734534E-4	0.0	1.9318053397031395E-5	0.0
12-13	0.0	2.1249858736734534E-4	0.0	1.9318053397031395E-5	0.0
14-15	0.0	3.380659344480494E-4	0.0	2.8977080095547095E-5	9.659026698515698E-6
16-17	0.0	3.477249611465651E-4	0.0	3.863610679406279E-5	1.9318053397031395E-5
18-19	0.0	3.960200946391436E-4	0.0	5.795416019109418E-5	1.9318053397031395E-5
20-21	0.0	4.443152281317221E-4	0.0	7.727221358812558E-5	1.9318053397031395E-5
22-23	0.0	4.7329230822726917E-4	0.0	1.2556734708070406E-4	2.8977080095547095E-5
24-25	0.0	5.602235485139105E-4	0.0	1.7386248057328255E-4	3.863610679406279E-5
26-27	0.0	5.602235485139105E-4	0.0	2.1249858736734534E-4	3.863610679406279E-5
28-29	0.0	5.602235485139105E-4	0.0	7.147679756901616E-4	3.863610679406279E-5
30-31	0.0	6.181777087050046E-4	0.0	0.0020187365799897807	3.863610679406279E-5
32-33	0.0	6.374957621020361E-4	0.0	0.00408576829347214	3.863610679406279E-5
34-35	0.0	7.34086029087193E-4	0.0	0.007166997810298647	5.795416019109418E-5
36-37	0.0	8.306762960723499E-4	0.0	0.011841966732380246	5.795416019109418E-5
38-39	0.0	8.403353227708656E-4	0.0	0.027325386530100908	5.795416019109418E-5
40-41	0.0	9.272665630575069E-4	0.0	0.043851981211261264	5.795416019109418E-5
42-43	0.0	9.465846164545383E-4	0.0	0.05207181293169813	5.795416019109418E-5
44-45	0.0	0.001052833910138211	0.0	0.062435948579205466	5.795416019109418E-5
46-47	0.0	0.0011397651504248523	0.0	0.07406541672421836	5.795416019109418E-5
48-49	0.0	0.001149424177123368	0.0	0.08493182176004853	5.795416019109418E-5
50-51	0.0	0.0014005588712847762	0.0	0.09705390026668573	7.727221358812558E-5
52-53	0.0	0.0014102178979832917	0.0	0.12894800642518456	7.727221358812558E-5
54-55	0.0	0.0014778310848729017	0.0	0.16483129061017038	9.659026698515697E-5
56-57	0.0	0.0015551032984610271	0.0	0.20926281342334258	1.1590832038218836E-4
58-59	0.0	0.0015647623251595429	0.0	0.39599111755904803	1.1590832038218836E-4
60-61	0.0	0.0015937394052550902	0.0	0.5608610442760125	1.1590832038218836E-4
62-63	0.0	0.0016227164853506372	0.0	0.5678541796057378	1.1590832038218836E-4
64-65	0.0	0.0016806706455417314	0.0	0.5733501657971933	1.1590832038218836E-4
66-67	0.0	0.0017096477256372785	0.0	0.5784984270275022	1.2556734708070406E-4
68-69	0.0	0.0017869199392254041	0.0	0.584226229859722	1.3522637377921977E-4
70-71	0.0	0.0017965789659239197	0.0	0.5887659724080243	1.3522637377921977E-4
72-73	0.0	0.0018062379926224352	0.0	0.5918858380316449	1.7386248057328255E-4
74-75	0.0	0.0018448740994164983	0.0	0.5945903655072293	1.7386248057328255E-4
76-77	0.0	0.0019318053397031393	0.0	0.597227279795924	1.8352150727179826E-4
78-79	0.0	0.0019897594998942334	0.0	0.599062494868642	1.9318053397031395E-4
80-81	0.0	0.0019897594998942334	0.0	0.5996806725773471	2.0283956066882963E-4
82-83	0.0	0.0019897594998942334	0.0	0.6000573746185892	2.1249858736734534E-4
84-85	0.0	0.0019897594998942334	0.0	0.6002698732059566	2.1249858736734534E-4
86-87	0.0	0.002047713660085328	0.0	0.6003857815263387	2.1249858736734534E-4
88	0.0	0.0022215761406586105	0.0	0.6004050995797358	2.1249858736734534E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5375	0.0	47.20003	1
GTATCAA	9540	0.0	36.706894	1
ATCAACG	10735	0.0	31.978767	3
TCAACGC	10820	0.0	31.727549	4
CAACGCA	11160	0.0	30.760939	5
AACGCAG	11485	0.0	29.927397	6
TATCAAC	11810	0.0	29.32322	2
ACGCAGA	13310	0.0	25.856014	7
GTACATG	14105	0.0	25.193373	1
TACATGG	13860	0.0	25.079323	2
CGCAGAG	13740	0.0	25.01549	8
ACATGGG	14085	0.0	24.005917	3
GTCAACG	795	0.0	23.839163	1
CTTATAC	6975	0.0	22.478258	1
CATGGGG	10170	0.0	22.235342	4
TATACAC	8610	0.0	21.061264	3
GCAGAGT	17190	0.0	20.0701	9
GCGCAGA	1160	0.0	19.679922	1
GAGTACT	9710	0.0	19.162731	12-13
TTATACA	9095	0.0	18.42266	2
>>END_MODULE