FastQCFastQC Report
Fri 27 May 2016
SRR2139753_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139753_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1766255
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16200.043.341811
GTATCAA26250.033.769461
ATCAACG28400.030.2858523
TCAACGC28700.029.9692754
CAACGCA29100.029.7081285
AACGCAG29750.029.206556
GTCAACG2200.027.9259171
TATCAAC32300.027.0366342
GTACATG40550.025.6483551
TACATGG39350.025.2037852
ACGCAGA35600.024.407167
ATCTCGT25750.024.09793394
CGCAGAG36400.023.8707398
ACATGGG41800.023.4115753
TAGATCG1202.9254466E-421.9417935
GAGTACA27100.021.3750291
TATCTCG29200.019.97570294
CATGGGG32750.019.9653554
GTGGTAT8600.019.3904191
GTATAGC5450.019.3248791
Produced by FastQC (version 0.11.5)