##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139753_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1766255
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.764162026434462	25.0	18.0	32.0	18.0	33.0
2	28.414625860931746	31.0	27.0	32.0	18.0	33.0
3	31.325481314985662	32.0	31.0	33.0	27.0	33.0
4	31.641325856119302	33.0	32.0	33.0	30.0	33.0
5	32.484679165805616	33.0	33.0	33.0	32.0	33.0
6	36.46402812730891	38.0	37.0	38.0	34.0	38.0
7	37.01755861979159	38.0	38.0	38.0	35.0	38.0
8	36.970601074023854	38.0	38.0	38.0	35.0	38.0
9	37.21073854001829	38.0	38.0	38.0	36.0	38.0
10-11	37.323695615865205	38.0	38.0	38.0	37.0	38.0
12-13	37.34395344953022	38.0	38.0	38.0	37.0	38.0
14-15	37.3389051977206	38.0	38.0	38.0	37.0	38.0
16-17	37.29059620496474	38.0	38.0	38.0	37.0	38.0
18-19	37.28328412375336	38.0	38.0	38.0	37.0	38.0
20-21	37.34737438008948	38.0	38.0	38.0	37.0	38.0
22-23	37.36833996949016	38.0	38.0	38.0	37.0	38.0
24-25	37.370088630433656	38.0	38.0	38.0	37.0	38.0
26-27	37.279209727506625	38.0	38.0	38.0	37.0	38.0
28-29	37.19379371286709	38.0	38.0	38.0	36.5	38.0
30-31	37.277074099694595	38.0	38.0	38.0	37.0	38.0
32-33	37.300149892238395	38.0	38.0	38.0	37.0	38.0
34-35	37.25381541195081	38.0	38.0	38.0	37.0	38.0
36-37	37.26137484207166	38.0	38.0	38.0	37.0	38.0
38-39	37.25502807806059	38.0	38.0	38.0	37.0	38.0
40-41	37.22807430158866	38.0	38.0	38.0	37.0	38.0
42-43	37.23027301169293	38.0	38.0	38.0	37.0	38.0
44-45	37.17156747284989	38.0	38.0	38.0	36.5	38.0
46-47	37.15442787011254	38.0	38.0	38.0	36.0	38.0
48-49	37.138380025184716	38.0	38.0	38.0	36.0	38.0
50-51	37.13664221419239	38.0	38.0	38.0	36.0	38.0
52-53	37.11626839747046	38.0	38.0	38.0	36.0	38.0
54-55	37.07207914884083	38.0	38.0	38.0	36.0	38.0
56-57	37.06727514937684	38.0	38.0	38.0	36.0	38.0
58-59	37.075087925302114	38.0	38.0	38.0	36.0	38.0
60-61	37.07858878761877	38.0	38.0	38.0	36.0	38.0
62-63	37.001252311078304	38.0	38.0	38.0	36.0	38.0
64-65	37.03024069147773	38.0	38.0	38.0	36.0	38.0
66-67	37.04559202849205	38.0	38.0	38.0	36.0	38.0
68-69	37.010533193387474	38.0	38.0	38.0	36.0	38.0
70-71	36.96086361009924	38.0	38.0	38.0	36.0	38.0
72-73	36.971096828347925	38.0	38.0	38.0	36.0	38.0
74-75	36.989602382838925	38.0	38.0	38.0	36.0	38.0
76-77	36.919369795552164	38.0	38.0	38.0	35.5	38.0
78-79	36.942250626424716	38.0	38.0	38.0	36.0	38.0
80-81	36.910236164160665	38.0	38.0	38.0	35.5	38.0
82-83	36.848774227362014	38.0	38.0	38.0	35.0	38.0
84-85	36.89150788822214	38.0	38.0	38.0	35.0	38.0
86-87	36.91788312497492	38.0	38.0	38.0	35.0	38.0
88-89	36.825120517327186	38.0	38.0	38.0	35.0	38.0
90-91	36.78224415645337	38.0	38.0	38.0	35.0	38.0
92-93	36.834954269960306	38.0	38.0	38.0	35.0	38.0
94-95	36.80097961322532	38.0	38.0	38.0	35.0	38.0
96-97	36.715564647218244	38.0	38.0	38.0	34.5	38.0
98-99	36.74103097675375	38.0	38.0	38.0	34.5	38.0
100	35.12324979977034	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	6.0
20	31.0
21	66.0
22	203.0
23	438.0
24	1017.0
25	1820.0
26	3184.0
27	4992.0
28	7400.0
29	10664.0
30	14710.0
31	19411.0
32	25587.0
33	35879.0
34	53733.0
35	102659.0
36	319507.0
37	1164945.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.53033961687299	15.350727952645569	12.644493575389737	24.474438855091705
2	14.885976131392276	20.56743852664369	41.513928072592094	23.032657269371942
3	18.36326011815961	25.721993709855035	30.190544400440476	25.72420177154488
4	12.177976667002593	17.8067254200132	38.74867374681104	31.266624166173155
5	13.41949086807887	37.37910636375269	36.031370914868006	13.170031853300435
6	30.384560727281375	37.66654644956628	18.78076081005566	13.168132013096681
7	26.839684439497375	32.266593592993985	23.05376236939874	17.839959598109896
8	24.617623163133295	36.407993183317245	21.521977290934775	17.452406362614685
9	25.649637226787753	16.399953574087547	21.050357960770107	36.9000512383546
10-11	24.19384516958197	26.56671884863737	29.2388981205998	20.00053786118086
12-13	24.854253774228525	23.92021537094021	29.248154994607233	21.977375860224033
14-15	21.911077392562227	25.454280384202733	26.50528944008651	26.129352783148523
16-17	22.003985834435007	28.666698749614294	27.154714353250238	22.17460106270046
18-19	22.03679536646747	27.901973384364094	28.616564425861498	21.44466682330694
20-21	22.506365263355164	27.258529056570875	28.524730966064947	21.710374714009014
22-23	22.279251043292426	27.29795401161897	28.288009575557417	22.13478536953119
24-25	22.132279768468646	27.319737212762252	28.47674281985258	22.07124019891652
26-27	22.19135005470348	27.456276856237444	28.411803946352084	21.940569142706995
28-29	22.096523977758398	27.38180785106703	28.407951166137615	22.11371700503696
30-31	22.229543932435107	27.219446966401854	28.488018323682475	22.06299077748056
32-33	21.980584366915007	27.53178025694688	28.399511540675487	22.088123835462625
34-35	22.280745085404163	27.41812757794257	28.371177580093377	21.929949756559893
36-37	22.1257573655904	27.398580578550806	28.494706842137624	21.98095521372117
38-39	21.93025031059789	27.415785323007448	28.54734959336518	22.106614773029477
40-41	22.279740584685403	27.213108417497928	28.515809905562	21.991341092254665
42-43	22.127891383782927	27.308806735198658	28.4500300367722	22.11327184424621
44-45	22.086764333727476	27.39322584722152	28.387156657938295	22.132853161112706
46-47	22.221529725221437	27.238170722134893	28.32761876315397	22.2126807894897
48-49	22.06569864402604	27.468599869235216	28.243091023660842	22.2226104630779
50-51	21.9984551546172	27.584821163725888	28.14116822149899	22.275555460157925
52-53	22.05747495186325	27.385494872084386	28.242517221994206	22.314512954058156
54-55	22.133763434304857	27.176482155382043	28.361650605120868	22.32810380519223
56-57	22.11596562727884	27.043567462938338	28.356956780557724	22.483510129225095
58-59	21.864547919768704	26.97884742737122	28.517507299818078	22.639097353041997
60-61	22.1228713351729	27.328385464739068	28.480319264397895	22.068423935690134
62-63	21.609470199637727	27.67458009240873	28.47186570706239	22.244084000891153
64-65	21.465969065030357	27.927499650315447	28.186829980780303	22.419701303873897
66-67	21.465808646315292	28.416172619285195	27.785091691714435	22.33292704268508
68-69	21.651870310599623	28.348563176394524	27.6478314503484	22.35173506265745
70-71	21.721196651833612	27.803717665328914	27.650858171439385	22.82422751139809
72-73	21.96469042162893	27.236376294140126	27.74072677421267	23.058206510018277
74-75	21.991867956241194	27.2837627186257	27.86622464056265	22.858144684570462
76-77	22.282035252646956	27.210136975607757	27.307940131799114	23.199887639946173
78-79	22.448545376296945	27.278131402109278	26.893412486440223	23.37991073515355
80-81	22.26122842574284	27.57905010562541	27.07954687968061	23.080174588951145
82-83	22.274091691309938	27.401147516508157	27.132472543159558	23.192288249022344
84-85	22.405195859013833	27.620945064478196	27.144273158631698	22.82958591787627
86-87	22.69754017640961	27.59528488666386	27.150787707894825	22.556387229031706
88-89	22.481386851287123	27.732737230116882	27.44828824699687	22.337587671599128
90-91	22.553024158199367	27.390812445335698	27.224433661823046	22.831729734641886
92-93	22.54847715269085	26.99848795302638	27.494587907576385	22.958446986706388
94-95	21.951709127307677	27.400416058825712	27.994083604999854	22.653791208866757
96-97	22.061111897015138	27.303176918300746	28.224426779799682	22.411284404884434
98-99	23.187941194170286	29.2411507581604	28.493001261487468	19.07790678618185
100	22.216888125496553	30.260009070644884	22.167916292743804	25.35518651111476
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	138.0
1	143.0
2	258.5
3	410.0
4	523.5
5	729.5
6	911.5
7	1113.5
8	1374.0
9	1538.5
10	1525.5
11	1486.0
12	1619.5
13	1924.0
14	2433.0
15	3045.0
16	3626.5
17	4026.5
18	4091.0
19	3950.0
20	3796.5
21	3724.5
22	3989.0
23	4820.5
24	6268.5
25	8181.5
26	10860.5
27	14200.5
28	17706.0
29	21757.0
30	25903.5
31	29903.5
32	34183.0
33	38279.0
34	41929.5
35	45636.5
36	50215.0
37	54530.5
38	57905.5
39	61122.0
40	64907.5
41	68182.0
42	70190.0
43	72373.5
44	75430.0
45	79189.5
46	79480.0
47	77837.0
48	77021.5
49	75373.0
50	73481.0
51	70762.0
52	66942.5
53	62848.5
54	58906.0
55	54025.0
56	48822.0
57	43939.0
58	38876.0
59	33331.5
60	27664.0
61	22486.0
62	17716.0
63	13654.0
64	10227.5
65	7392.0
66	5400.0
67	4173.5
68	3143.5
69	2284.5
70	1667.5
71	1201.0
72	844.0
73	555.0
74	368.0
75	258.5
76	180.0
77	109.5
78	67.0
79	46.5
80	31.0
81	22.0
82	16.5
83	9.5
84	5.0
85	2.0
86	2.5
87	3.0
88	2.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	1.1323393281264595E-4
3	0.0
4	5.6616966406322976E-5
5	0.0018683598914086585
6	3.397017984379379E-4
7	5.6616966406322976E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0011046468812136387
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	2.9007014476240646E-5
44-45	2.9188609203635497E-5
46-47	5.879688059029716E-5
48-49	2.9611503002902517E-4
50-51	5.664202241831625E-4
52-53	1.7922431715535164E-4
54-55	4.7815332404719727E-4
56-57	6.577666847751828E-4
58-59	4.486653551679818E-4
60-61	9.019439598165927E-5
62-63	5.751296387610738E-4
64-65	9.142071447116774E-5
66-67	2.1491398681717605E-4
68-69	6.19263299608254E-4
70-71	1.56170060450307E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0010051099791339169
90-91	0.0
92-93	3.4070447464221774E-5
94-95	3.437365190833922E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	23.0
20-21	50.0
22-23	97.0
24-25	132.0
26-27	436.0
28-29	1337.0
30-31	3350.0
32-33	4369.0
34-35	6045.0
36-37	9266.0
38-39	5309.0
40-41	9753.0
42-43	10172.0
44-45	12039.0
46-47	12362.0
48-49	11619.0
50-51	5817.0
52-53	779.0
54-55	804.0
56-57	692.0
58-59	5852.0
60-61	11456.0
62-63	10981.0
64-65	11644.0
66-67	13499.0
68-69	13740.0
70-71	15826.0
72-73	11718.0
74-75	11315.0
76-77	11763.0
78-79	12064.0
80-81	12002.0
82-83	11286.0
84-85	11368.0
86-87	11854.0
88-89	12189.0
90-91	12594.0
92-93	12580.0
94-95	15445.0
96-97	60022.0
98-99	139034.0
100-101	1243572.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.93599599761451
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.98169327702473	86.40300576965579
2	4.662972390028724	8.573900219733353
3	0.8341673916176077	2.3007002993129073
4	0.26669256585822176	0.9807458666934006
5	0.10641611779719287	0.48917358899421987
6	0.05396279761554253	0.29766741273615605
7	0.030428678428411574	0.19582436007450016
8	0.017430505128600518	0.1281992679791533
9	0.0122380322906515	0.10126041189228122
>10	0.033168890432982504	0.45280168282126043
>50	6.047404555973308E-4	0.039757847350262325
>100	2.2461332178438267E-4	0.036963272756681265
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.661696640632298E-5	0.0
5	0.0	0.0	0.0	5.661696640632298E-5	0.0
6	0.0	0.0	0.0	5.661696640632298E-5	0.0
7	0.0	0.0	0.0	5.661696640632298E-5	0.0
8	5.661696640632298E-5	0.0	0.0	5.661696640632298E-5	0.0
9	5.661696640632298E-5	5.661696640632298E-5	0.0	5.661696640632298E-5	0.0
10-11	5.661696640632298E-5	5.661696640632298E-5	0.0	5.661696640632298E-5	0.0
12-13	5.661696640632298E-5	5.661696640632298E-5	0.0	5.661696640632298E-5	0.0
14-15	5.661696640632298E-5	1.1323393281264597E-4	0.0	5.661696640632298E-5	5.661696640632298E-5
16-17	8.492544960948448E-5	1.4154241601580745E-4	0.0	5.661696640632298E-5	5.661696640632298E-5
18-19	1.1323393281264597E-4	1.6985089921896896E-4	0.0	5.661696640632298E-5	1.1323393281264597E-4
20-21	1.1323393281264597E-4	1.6985089921896896E-4	0.0	1.1323393281264597E-4	1.1323393281264597E-4
22-23	1.1323393281264597E-4	2.547763488284534E-4	0.0	1.1323393281264597E-4	1.6985089921896896E-4
24-25	1.1323393281264597E-4	3.113933152347764E-4	0.0	1.6985089921896896E-4	2.2646786562529193E-4
26-27	1.1323393281264597E-4	3.397017984379379E-4	0.0	2.830848320316149E-4	2.2646786562529193E-4
28-29	1.1323393281264597E-4	3.397017984379379E-4	0.0	9.341799457043292E-4	2.2646786562529193E-4
30-31	1.1323393281264597E-4	4.5293573125058386E-4	0.0	0.002745922870706665	2.547763488284534E-4
32-33	1.1323393281264597E-4	4.5293573125058386E-4	0.0	0.006510951136727143	2.830848320316149E-4
34-35	1.4154241601580745E-4	4.8124421445374537E-4	0.0	0.010191053953138136	2.830848320316149E-4
36-37	1.6985089921896896E-4	5.095526976569069E-4	0.0	0.02015564004065098	2.830848320316149E-4
38-39	1.6985089921896896E-4	5.661696640632298E-4	0.0	0.17520120254436647	2.830848320316149E-4
40-41	2.2646786562529193E-4	5.661696640632298E-4	0.0	0.33763527916410707	2.830848320316149E-4
42-43	2.2646786562529193E-4	5.944781472663913E-4	0.0	0.3627165952821082	3.113933152347764E-4
44-45	2.2646786562529193E-4	6.227866304695528E-4	0.0	0.3974227956891842	3.397017984379379E-4
46-47	2.830848320316149E-4	6.227866304695528E-4	0.0	0.4332330269411835	3.397017984379379E-4
48-49	2.830848320316149E-4	6.227866304695528E-4	0.0	0.4653065384103654	3.397017984379379E-4
50-51	2.830848320316149E-4	7.360205632821988E-4	0.0	0.499927813367832	3.397017984379379E-4
52-53	2.830848320316149E-4	7.360205632821988E-4	0.0	0.923281179671112	3.6801028164109943E-4
54-55	2.830848320316149E-4	7.360205632821988E-4	0.0	1.4963864221191163	3.963187648442609E-4
56-57	2.830848320316149E-4	7.360205632821988E-4	0.0	2.1149833970746013	3.963187648442609E-4
58-59	3.113933152347764E-4	7.360205632821988E-4	0.0	2.7429221714871295	3.963187648442609E-4
60-61	3.397017984379379E-4	7.360205632821988E-4	0.0	2.9094609781713285	3.963187648442609E-4
62-63	3.397017984379379E-4	7.360205632821988E-4	0.0	2.916170088690478	3.963187648442609E-4
64-65	3.397017984379379E-4	8.492544960948447E-4	0.0	2.9220299447135325	3.963187648442609E-4
66-67	3.397017984379379E-4	8.492544960948447E-4	0.0	2.9271254716901014	3.963187648442609E-4
68-69	3.397017984379379E-4	8.492544960948447E-4	0.0	2.9313434356873724	3.963187648442609E-4
70-71	3.397017984379379E-4	8.492544960948447E-4	0.0	2.9352216978862056	3.963187648442609E-4
72-73	3.397017984379379E-4	8.492544960948447E-4	0.0	2.9385054819377725	4.5293573125058386E-4
74-75	3.397017984379379E-4	9.058714625011677E-4	0.0	2.941591106606917	4.5293573125058386E-4
76-77	3.397017984379379E-4	9.058714625011677E-4	0.0	2.944337029477624	4.5293573125058386E-4
78-79	3.397017984379379E-4	9.058714625011677E-4	0.0	2.9459223045370004	4.5293573125058386E-4
80-81	3.963187648442609E-4	9.058714625011677E-4	0.0	2.9464884742010637	4.8124421445374537E-4
82-83	3.963187648442609E-4	9.058714625011677E-4	0.0	2.9469131014491112	5.095526976569069E-4
84-85	3.963187648442609E-4	9.058714625011677E-4	0.0	2.9470263353819237	5.095526976569069E-4
86-87	3.963187648442609E-4	9.058714625011677E-4	0.0	2.947054643865127	5.095526976569069E-4
88	3.963187648442609E-4	9.058714625011677E-4	0.0	2.9470829523483304	5.095526976569069E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1620	0.0	43.34181	1
GTATCAA	2625	0.0	33.76946	1
ATCAACG	2840	0.0	30.285852	3
TCAACGC	2870	0.0	29.969275	4
CAACGCA	2910	0.0	29.708128	5
AACGCAG	2975	0.0	29.20655	6
GTCAACG	220	0.0	27.925917	1
TATCAAC	3230	0.0	27.036634	2
GTACATG	4055	0.0	25.648355	1
TACATGG	3935	0.0	25.203785	2
ACGCAGA	3560	0.0	24.40716	7
ATCTCGT	2575	0.0	24.097933	94
CGCAGAG	3640	0.0	23.870739	8
ACATGGG	4180	0.0	23.411575	3
TAGATCG	120	2.9254466E-4	21.941793	5
GAGTACA	2710	0.0	21.375029	1
TATCTCG	2920	0.0	19.975702	94
CATGGGG	3275	0.0	19.965355	4
GTGGTAT	860	0.0	19.390419	1
GTATAGC	545	0.0	19.324879	1
>>END_MODULE