FastQCFastQC Report
Fri 27 May 2016
SRR2139752_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139752_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7867615
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72450.044.1442071
GTATCAA127100.036.90381
TCAACGC133150.034.1455964
ATCAACG137900.033.0643653
CAACGCA139150.032.547855
AACGCAG144900.031.405636
TATCAAC153050.030.07652
ACGCAGA169950.026.6993717
CGCAGAG173650.026.155448
GTACATG191450.024.0211281
TACATGG194950.022.761762
ACATGGG194450.022.416393
GCAGAGT221050.020.54699
CATGGGG145100.020.1773094
GAGTACT122200.018.70880912-13
CAGAGTA214550.017.9563610-11
AGAGTAC190000.017.4864810-11
GTGGTAT39400.016.2790581
ATGGGGG84900.016.2400575
GTACTTT147750.015.44411714-15
Produced by FastQC (version 0.11.5)