##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139752_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7867615
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27972377906138	33.0	33.0	34.0	31.0	34.0
2	31.320894070185183	33.0	32.0	33.0	27.0	34.0
3	32.51543167783375	33.0	33.0	33.0	31.0	34.0
4	32.92738828730181	33.0	33.0	34.0	32.0	34.0
5	33.160324189732215	34.0	33.0	34.0	33.0	34.0
6	37.079497026735545	38.0	37.0	38.0	36.0	38.0
7	37.13085769956969	38.0	38.0	38.0	36.0	38.0
8	37.33527250634405	38.0	38.0	38.0	37.0	38.0
9	37.322267929988946	38.0	38.0	38.0	37.0	38.0
10-11	37.37698203330997	38.0	38.0	38.0	37.0	38.0
12-13	37.387159704688145	38.0	38.0	38.0	37.0	38.0
14-15	37.392076251824726	38.0	38.0	38.0	37.0	38.0
16-17	37.35419800028344	38.0	38.0	38.0	37.0	38.0
18-19	37.33248354170864	38.0	38.0	38.0	37.0	38.0
20-21	37.39126953745793	38.0	38.0	38.0	37.0	38.0
22-23	37.40695044292559	38.0	38.0	38.0	37.0	38.0
24-25	37.40869997128845	38.0	38.0	38.0	37.0	38.0
26-27	37.313207568440404	38.0	38.0	38.0	37.0	38.0
28-29	37.21798639568485	38.0	38.0	38.0	37.0	38.0
30-31	37.30203302478146	38.0	38.0	38.0	37.0	38.0
32-33	37.32481297276297	38.0	38.0	38.0	37.0	38.0
34-35	37.284360620424124	38.0	38.0	38.0	37.0	38.0
36-37	37.29158413594297	38.0	38.0	38.0	37.0	38.0
38-39	37.295668999487134	38.0	38.0	38.0	37.0	38.0
40-41	37.25867392447162	38.0	38.0	38.0	37.0	38.0
42-43	37.26376608051247	38.0	38.0	38.0	37.0	38.0
44-45	37.20958265189136	38.0	38.0	38.0	37.0	38.0
46-47	37.19822333203856	38.0	38.0	38.0	37.0	38.0
48-49	37.185474040171655	38.0	38.0	38.0	37.0	38.0
50-51	37.18902275104975	38.0	38.0	38.0	37.0	38.0
52-53	37.174834252022364	38.0	38.0	38.0	36.5	38.0
54-55	37.1280924117618	38.0	38.0	38.0	36.0	38.0
56-57	37.13363673499455	38.0	38.0	38.0	36.0	38.0
58-59	37.148739285867244	38.0	38.0	38.0	36.0	38.0
60-61	37.16199286948343	38.0	38.0	38.0	36.0	38.0
62-63	37.09278699186824	38.0	38.0	38.0	36.0	38.0
64-65	37.123064754833855	38.0	38.0	38.0	36.0	38.0
66-67	37.14963107843809	38.0	38.0	38.0	36.0	38.0
68-69	37.1232154551611	38.0	38.0	38.0	36.0	38.0
70-71	37.075184218521436	38.0	38.0	38.0	36.0	38.0
72-73	37.08915072229436	38.0	38.0	38.0	36.0	38.0
74-75	37.11381546271842	38.0	38.0	38.0	36.0	38.0
76-77	37.055603950344434	38.0	38.0	38.0	36.0	38.0
78-79	37.08300704647187	38.0	38.0	38.0	36.0	38.0
80-81	37.06470885324387	38.0	38.0	38.0	36.0	38.0
82-83	37.010578268852896	38.0	38.0	38.0	36.0	38.0
84-85	37.04895402593605	38.0	38.0	38.0	36.0	38.0
86-87	37.07585465432142	38.0	38.0	38.0	36.0	38.0
88-89	36.986513978411615	38.0	38.0	38.0	36.0	38.0
90-91	36.95028654706287	38.0	38.0	38.0	36.0	38.0
92-93	37.011786900530325	38.0	38.0	38.0	36.0	38.0
94-95	36.99893914404826	38.0	38.0	38.0	36.0	38.0
96-97	36.949179788978824	38.0	38.0	38.0	36.0	38.0
98-99	36.95963119967821	38.0	38.0	38.0	35.5	38.0
100	35.51745965537764	38.0	36.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	3.0
19	20.0
20	51.0
21	166.0
22	470.0
23	1404.0
24	3294.0
25	6511.0
26	11619.0
27	18827.0
28	28618.0
29	40729.0
30	56161.0
31	75579.0
32	99182.0
33	135294.0
34	199667.0
35	364708.0
36	1017345.0
37	5807965.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.18527469887634	20.118384033789148	12.749746905510756	24.946594361823752
2	15.115178590494086	21.573088033872555	40.45506588533643	22.85666749029692
3	18.00521504928749	26.804921186407825	30.275629908174206	24.91423385613048
4	12.360902855796288	17.852314291513828	39.5518945835287	30.234888269161182
5	13.070254166851502	37.77235680799391	36.22524925988806	12.932139765266529
6	29.6948414392122	38.340356668668974	19.127986209740172	12.836815682378653
7	26.140486409592345	32.57019160080167	24.096497944483254	17.192824045122737
8	24.417526785436248	36.38543573878488	21.974168791940123	17.222868683838747
9	25.922328431170055	16.254964179106373	21.259873544905286	36.56283384481828
10-11	23.904658527393625	26.635441617313504	29.812687834877533	19.647212020415335
12-13	24.768548028849914	24.288161024656137	29.60853447963582	21.334756466858128
14-15	21.968773255935883	25.609565287574444	26.864005165478993	25.557656291010684
16-17	21.801238367662883	28.652673014630224	27.542374658648143	22.00371395905875
18-19	21.868749296959752	28.112693618078666	28.865958743532826	21.152598341428757
20-21	22.526480410457484	27.42773003009591	28.66814091465233	21.377648644794277
22-23	22.25090498137287	27.346694649198728	28.552001839515494	21.85039852991291
24-25	22.03383625615328	27.412155873448224	28.631186740064063	21.92282113033444
26-27	22.04629401453045	27.587730636295284	28.54024858038558	21.82572676878869
28-29	22.03495474878643	27.52891203414343	28.551399094098283	21.884734122971853
30-31	22.19814196957233	27.473936241368563	28.506291335474803	21.821630453584305
32-33	21.984813885365735	27.630375493509195	28.520416614015907	21.86439400710916
34-35	22.1531385442889	27.58944866721497	28.478734677540114	21.778678110956015
36-37	22.143526518895595	27.53261615034918	28.46939625671505	21.854461074040174
38-39	21.98481924730245	27.642765202781206	28.567259982878674	21.80515556703767
40-41	22.20624830393663	27.51879827867873	28.478352205264684	21.796601212119956
42-43	22.056650412337113	27.587902954416695	28.378104219059153	21.977342414187046
44-45	22.139905023992956	27.633739969358984	28.2991616469103	21.92719335973776
46-47	22.18417234661148	27.607390975630075	28.15891300693017	22.04952367082828
48-49	22.07466496019785	27.666499724688485	28.226285413947604	22.032549901166053
50-51	22.082796572570945	27.767179907953327	28.14929120852688	22.00073231094885
52-53	22.222332709669026	27.68966739836978	28.04709578184385	22.040904110117342
54-55	22.23036880626427	27.608436254710234	28.09611876152074	22.065076177504757
56-57	22.121710241277913	27.723244713866848	28.097749160652658	22.057295884202585
58-59	22.1518446330531	27.687644862576555	28.07473148066974	22.085779023700603
60-61	22.164018779350478	27.683252239182014	28.135111176444315	22.017617805023193
62-63	22.190270016794123	27.700754797279842	28.1038262252429	22.005148960683137
64-65	22.16579986976098	27.653431831524838	28.076750440389315	22.104017858324866
66-67	22.15785639200039	27.757445142742394	27.96764294878175	22.117055516475467
68-69	22.168979410380494	27.712825879524104	28.002769684149914	22.11542502594548
70-71	22.19811597051506	27.567445633419034	28.005526843126987	22.228911552938914
72-73	22.223666017722774	27.59624238133231	27.940938608112052	22.23915299283287
74-75	22.104958928522368	27.707709444417162	27.914637033389862	22.272694593670607
76-77	22.281405651746073	27.611057926934183	27.853784444216316	22.253751977103427
78-79	22.254558175027718	27.636797898312558	27.78295315514891	22.325690771510807
80-81	22.271217146928816	27.68134753861214	27.728403488416376	22.319031826042668
82-83	22.309851580474437	27.665603757143774	27.77503080696439	22.249513855417398
84-85	22.32762178751699	27.688367591948644	27.754820648475437	22.229189972058933
86-87	22.221576886169114	27.649920572255926	27.86945938351431	22.259043158060646
88-89	22.31138803871025	27.686361154068432	27.782213712099473	22.220037095121842
90-91	22.358977962048936	27.70688310805599	27.76195316563097	22.172185764264107
92-93	22.388861667683422	27.676969520921663	27.8029439233418	22.131224888053115
94-95	22.313799097381278	27.729433826777626	27.787964913007656	22.168802162833433
96-97	22.401176952150013	27.834797333375455	27.855542670919775	21.908483043554757
98-99	23.443583868298152	29.737289925162784	28.10025440216676	18.718871804372302
100	22.346610509369143	30.62748072863665	22.106726458679145	24.91918230331506
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1030.0
1	955.0
2	1436.5
3	2188.0
4	2634.5
5	3381.5
6	4212.0
7	5268.5
8	6615.5
9	7371.5
10	7269.0
11	7213.5
12	7937.0
13	9442.5
14	12048.5
15	14843.0
16	16749.5
17	17889.0
18	18009.0
19	17245.0
20	16573.5
21	17118.0
22	19584.5
23	24292.5
24	31659.0
25	41672.0
26	54571.5
27	69756.0
28	86331.0
29	105602.0
30	125303.5
31	144620.5
32	165557.5
33	186054.5
34	205590.5
35	223714.0
36	242401.5
37	260026.5
38	274237.0
39	287472.0
40	300784.5
41	312644.5
42	320622.0
43	327434.5
44	332784.0
45	336032.5
46	337045.5
47	334406.0
48	329432.0
49	322608.0
50	314379.0
51	302435.5
52	287794.0
53	271315.5
54	251696.5
55	232243.0
56	212202.5
57	190782.0
58	169636.0
59	146552.0
60	120990.0
61	96867.0
62	76050.0
63	58273.5
64	43648.5
65	32359.5
66	24065.5
67	18459.5
68	14123.0
69	10273.0
70	7490.5
71	5418.0
72	3743.0
73	2564.0
74	1752.5
75	1127.0
76	705.5
77	440.5
78	291.0
79	181.5
80	114.5
81	74.5
82	52.0
83	37.0
84	26.5
85	16.0
86	7.5
87	3.0
88	2.0
89	1.5
90	1.5
91	2.5
92	2.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	8.897232515825953E-5
3	0.0
4	2.5420664330931294E-5
5	0.002847114405064305
6	4.194409614603663E-4
7	8.897232515825953E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0010872533532387593
30-31	0.0
32-33	0.0
34-35	6.390981864438583E-6
36-37	0.0
38-39	0.0
40-41	0.0
42-43	6.512933644147895E-6
44-45	6.5514244893295634E-6
46-47	6.5955751605362994E-6
48-49	8.631488173466884E-5
50-51	4.476698350777646E-4
52-53	2.0156453044769968E-4
54-55	3.040321144393094E-4
56-57	4.417508666472387E-4
58-59	3.3507959952653937E-4
60-61	4.817403876275855E-5
62-63	3.462265563939701E-4
64-65	9.754678204314243E-5
66-67	8.416883173451131E-5
68-69	4.8736936382052376E-4
70-71	9.24791409740643E-5
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	9.776137811713858E-4
90-91	0.0
92-93	7.696852980025204E-6
94-95	6.207721054812858E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	72.0
20-21	192.0
22-23	324.0
24-25	482.0
26-27	1696.0
28-29	5199.0
30-31	13161.0
32-33	17557.0
34-35	24537.0
36-37	37301.0
38-39	40835.0
40-41	39161.0
42-43	42846.0
44-45	50521.0
46-47	50815.0
48-49	48400.0
50-51	42963.0
52-53	40239.0
54-55	43469.0
56-57	44565.0
58-59	46311.0
60-61	45284.0
62-63	44183.0
64-65	46641.0
66-67	49421.0
68-69	50235.0
70-71	49552.0
72-73	46296.0
74-75	46581.0
76-77	48349.0
78-79	50368.0
80-81	49443.0
82-83	47155.0
84-85	45809.0
86-87	47602.0
88-89	49982.0
90-91	51280.0
92-93	51176.0
94-95	62798.0
96-97	238831.0
98-99	550775.0
100-101	5605208.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.56705551941182
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.16357026106147	79.82925418398169
2	6.406773062392823	11.220445049096488
3	1.423231961092327	3.738846964619194
4	0.48094481474956374	1.6845968517979308
5	0.208459566320152	0.912709520875463
6	0.1156436189308384	0.6075942719629458
7	0.06332633713578055	0.388171061586692
8	0.03561824087130147	0.24951875807048313
9	0.023660654730374743	0.1864704479760323
>10	0.07712064262441302	1.0371615683403892
>50	0.0013067358025769095	0.07179919388036878
>100	3.0125076934364145E-4	0.04296136972192649
>500	4.285351904168094E-5	0.030470758090480315
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.2710332165465647E-5	0.0	0.0	0.0
3	0.0	2.5420664330931294E-5	0.0	0.0	0.0
4	0.0	8.897232515825952E-5	0.0	2.5420664330931294E-5	0.0
5	0.0	1.0168265732372517E-4	0.0	2.5420664330931294E-5	0.0
6	0.0	1.1439298948919082E-4	0.0	2.5420664330931294E-5	0.0
7	0.0	1.1439298948919082E-4	0.0	2.5420664330931294E-5	0.0
8	0.0	1.2710332165465646E-4	0.0	2.5420664330931294E-5	0.0
9	0.0	2.0336531464745035E-4	0.0	2.5420664330931294E-5	0.0
10-11	0.0	2.0336531464745035E-4	0.0	4.448616257912976E-5	0.0
12-13	0.0	2.0336531464745035E-4	0.0	5.084132866186259E-5	0.0
14-15	0.0	2.2878597897838164E-4	0.0	5.084132866186259E-5	0.0
16-17	0.0	2.4149631114384727E-4	0.0	5.084132866186259E-5	1.2710332165465647E-5
18-19	0.0	2.7962730764024424E-4	0.0	5.084132866186259E-5	2.5420664330931294E-5
20-21	0.0	3.2411347021937397E-4	0.0	6.355166082732823E-5	2.5420664330931294E-5
22-23	1.2710332165465647E-5	3.5588930063303807E-4	0.0	9.532749124099235E-5	3.1775830413664115E-5
24-25	1.9065498248198472E-5	4.0037546321216786E-4	0.0	1.5252398598558778E-4	3.813099649639694E-5
26-27	3.1775830413664115E-5	4.1944096146036633E-4	0.0	2.2243081289564882E-4	3.813099649639694E-5
28-29	3.813099649639694E-5	4.257961275430991E-4	0.0	6.228062761078167E-4	3.813099649639694E-5
30-31	3.813099649639694E-5	4.702822901222289E-4	0.0	0.0019891669838953737	3.813099649639694E-5
32-33	3.813099649639694E-5	4.702822901222289E-4	0.0	0.005090488032268991	3.813099649639694E-5
34-35	4.448616257912976E-5	5.846752796114197E-4	0.0	0.008255360741469937	3.813099649639694E-5
36-37	6.355166082732823E-5	6.927131030178776E-4	0.0	0.013676317410041036	3.813099649639694E-5
38-39	8.26171590755267E-5	7.117786012660761E-4	0.0	0.02726366249492381	3.813099649639694E-5
40-41	8.897232515825952E-5	7.562647638452059E-4	0.0	0.04247157493090346	3.813099649639694E-5
42-43	8.897232515825952E-5	7.8804059425887E-4	0.0	0.05118450763033015	3.813099649639694E-5
44-45	1.0168265732372517E-4	8.770129194171296E-4	0.0	0.06156884900951559	3.813099649639694E-5
46-47	1.1439298948919082E-4	9.405645802444578E-4	0.0	0.07325599943566125	3.813099649639694E-5
48-49	1.2710332165465646E-4	9.532749124099235E-4	0.0	0.08575025595431399	3.813099649639694E-5
50-51	1.3981365382012212E-4	0.001074023067981847	0.0	0.09711329291024028	3.813099649639694E-5
52-53	1.3981365382012212E-4	0.00108037823406458	0.0	0.12312498768686572	4.448616257912976E-5
54-55	1.4616881990285493E-4	0.001112154064478244	0.0	0.15358529872140414	5.084132866186259E-5
56-57	1.5252398598558775E-4	0.0011566402270573738	0.0	0.19551032936919258	5.719649474459541E-5
58-59	1.5252398598558775E-4	0.0012138367218019691	0.0	0.2299235537071908	6.990682691006105E-5
60-61	1.652343181510534E-4	0.0012837435487120302	0.0	0.24767353257626357	7.626199299279387E-5
62-63	1.652343181510534E-4	0.0013218745452084272	0.0	0.2614451774775456	7.626199299279387E-5
64-65	1.652343181510534E-4	0.0014235572025321523	0.0	0.26629416919867077	7.626199299279387E-5
66-67	1.7158948423378622E-4	0.0014235572025321523	0.0	0.2709525059373139	7.626199299279387E-5
68-69	1.7794465031651904E-4	0.0014553330329458165	0.0	0.2754646738560542	7.626199299279387E-5
70-71	1.8429981639925188E-4	0.001474398531194015	0.0	0.27979889712447803	7.626199299279387E-5
72-73	2.0336531464745035E-4	0.001474398531194015	0.0	0.28315442481616093	1.0168265732372517E-4
74-75	2.0336531464745035E-4	0.0014998191955249464	0.0	0.28565200508667493	1.0168265732372517E-4
76-77	2.0336531464745035E-4	0.00157608118851774	0.0	0.2878191167208868	1.0168265732372517E-4
78-79	2.1607564681291598E-4	0.0016142121850141371	0.0	0.28941426340765275	1.0168265732372517E-4
80-81	2.1607564681291598E-4	0.00162056735109687	0.0	0.2899099663621059	1.2710332165465646E-4
82-83	2.1607564681291598E-4	0.0016332776832623354	0.0	0.29030398665923535	1.3981365382012212E-4
84-85	2.1607564681291598E-4	0.001652343181510534	0.0	0.29049464164171734	1.3981365382012212E-4
86-87	2.1607564681291598E-4	0.0017667361709997249	0.0	0.29052006230604827	1.3981365382012212E-4
88	2.1607564681291598E-4	0.0018938394926543813	0.0	0.29052006230604827	1.5252398598558775E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7245	0.0	44.144207	1
GTATCAA	12710	0.0	36.9038	1
TCAACGC	13315	0.0	34.145596	4
ATCAACG	13790	0.0	33.064365	3
CAACGCA	13915	0.0	32.54785	5
AACGCAG	14490	0.0	31.40563	6
TATCAAC	15305	0.0	30.0765	2
ACGCAGA	16995	0.0	26.699371	7
CGCAGAG	17365	0.0	26.15544	8
GTACATG	19145	0.0	24.021128	1
TACATGG	19495	0.0	22.76176	2
ACATGGG	19445	0.0	22.41639	3
GCAGAGT	22105	0.0	20.5469	9
CATGGGG	14510	0.0	20.177309	4
GAGTACT	12220	0.0	18.708809	12-13
CAGAGTA	21455	0.0	17.95636	10-11
AGAGTAC	19000	0.0	17.48648	10-11
GTGGTAT	3940	0.0	16.279058	1
ATGGGGG	8490	0.0	16.240057	5
GTACTTT	14775	0.0	15.444117	14-15
>>END_MODULE