FastQCFastQC Report
Fri 27 May 2016
SRR2139751_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139751_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10228795
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108550.046.5818061
GTATCAA187250.034.524091
TCAACGC204600.030.982324
CTTATAC112650.030.3089071
ATCAACG208150.030.3082033
CAACGCA212500.029.7897285
AACGCAG220700.028.7018366
TATCAAC226000.028.1444592
TATACAC162250.024.4616663
ACGCAGA262900.024.0614997
CGCAGAG266050.023.727648
GTACATG263450.022.7128031
TACATGG260900.022.3203222
TTATACA161450.022.033342
ACATGGG263650.021.2658023
ACATCTC179500.019.8410958
ACACATC178100.019.608116
GCAGAGT324850.019.4194439
GTGGTAT59450.018.8765741
CATGGGG168950.018.4906564
Produced by FastQC (version 0.11.5)