FastQCFastQC Report
Fri 27 May 2016
SRR2139750_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139750_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7218187
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69200.047.639381
GTATCAA119050.033.70951
TCAACGC128100.030.2985954
ATCAACG130000.029.8557723
CAACGCA133100.029.1604065
AACGCAG137900.028.208566
TATCAAC146750.026.927282
ACGCAGA165600.023.4370167
CGCAGAG168750.023.0516198
GTACATG170350.022.5001431
TACATGG168000.021.9253042
ACATGGG167800.021.3751473
CTTATAC81750.020.1629681
CATGGGG121500.018.450364
GCAGAGT210850.018.4489469
GAGTACT124750.018.1102512-13
CAGAGTA204300.017.40532310-11
TATACAC115950.017.248513
TATGCCG52400.017.20756542-43
AGAGTAC183350.016.91289710-11
Produced by FastQC (version 0.11.5)