##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139750_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7218187
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.270540372533986	33.0	33.0	34.0	31.0	34.0
2	31.36625194110377	33.0	32.0	33.0	27.0	34.0
3	32.50659383027899	33.0	33.0	33.0	31.0	34.0
4	32.94085827923272	33.0	33.0	34.0	32.0	34.0
5	33.17390156281626	34.0	33.0	34.0	33.0	34.0
6	37.08411973810044	38.0	37.0	38.0	36.0	38.0
7	37.15313540643932	38.0	38.0	38.0	36.0	38.0
8	37.34793764694653	38.0	38.0	38.0	37.0	38.0
9	37.33613302066017	38.0	38.0	38.0	37.0	38.0
10-11	37.39162236999402	38.0	38.0	38.0	37.0	38.0
12-13	37.401404327707226	38.0	38.0	38.0	37.0	38.0
14-15	37.40625755470175	38.0	38.0	38.0	37.0	38.0
16-17	37.3629949598147	38.0	38.0	38.0	37.0	38.0
18-19	37.343206472761096	38.0	38.0	38.0	37.0	38.0
20-21	37.40286157745547	38.0	38.0	38.0	37.0	38.0
22-23	37.41791891651384	38.0	38.0	38.0	37.0	38.0
24-25	37.421210033297214	38.0	38.0	38.0	37.0	38.0
26-27	37.326734383804975	38.0	38.0	38.0	37.0	38.0
28-29	37.230586252236264	38.0	38.0	38.0	37.0	38.0
30-31	37.31250845868718	38.0	38.0	38.0	37.0	38.0
32-33	37.33501534631864	38.0	38.0	38.0	37.0	38.0
34-35	37.28861433204125	38.0	38.0	38.0	37.0	38.0
36-37	37.29690517680805	38.0	38.0	38.0	37.0	38.0
38-39	37.284832575884586	38.0	38.0	38.0	37.0	38.0
40-41	37.26213109918547	38.0	38.0	38.0	37.0	38.0
42-43	37.26864177024914	38.0	38.0	38.0	37.0	38.0
44-45	37.211739139276105	38.0	38.0	38.0	37.0	38.0
46-47	37.1959029492117	38.0	38.0	38.0	37.0	38.0
48-49	37.179514798664336	38.0	38.0	38.0	37.0	38.0
50-51	37.179021625403536	38.0	38.0	38.0	37.0	38.0
52-53	37.1660875010743	38.0	38.0	38.0	36.5	38.0
54-55	37.116408716094845	38.0	38.0	38.0	36.0	38.0
56-57	37.12051671880539	38.0	38.0	38.0	36.0	38.0
58-59	37.13500416125341	38.0	38.0	38.0	36.0	38.0
60-61	37.14457715208429	38.0	38.0	38.0	36.0	38.0
62-63	37.07383580629179	38.0	38.0	38.0	36.0	38.0
64-65	37.106377135377116	38.0	38.0	38.0	36.0	38.0
66-67	37.125791396251614	38.0	38.0	38.0	36.0	38.0
68-69	37.09631013181321	38.0	38.0	38.0	36.0	38.0
70-71	37.05019278212137	38.0	38.0	38.0	36.0	38.0
72-73	37.064723120734115	38.0	38.0	38.0	36.0	38.0
74-75	37.08765496360532	38.0	38.0	38.0	36.0	38.0
76-77	37.02902850082137	38.0	38.0	38.0	36.0	38.0
78-79	37.05662893548166	38.0	38.0	38.0	36.0	38.0
80-81	37.03553502366529	38.0	38.0	38.0	36.0	38.0
82-83	36.982957909208736	38.0	38.0	38.0	36.0	38.0
84-85	37.0187157247058	38.0	38.0	38.0	36.0	38.0
86-87	37.04512311897374	38.0	38.0	38.0	36.0	38.0
88-89	36.957252429739896	38.0	38.0	38.0	36.0	38.0
90-91	36.919072111507404	38.0	38.0	38.0	36.0	38.0
92-93	36.97637610138097	38.0	38.0	38.0	36.0	38.0
94-95	36.96000111057302	38.0	38.0	38.0	36.0	38.0
96-97	36.90928832739819	38.0	38.0	38.0	35.0	38.0
98-99	36.91976911836823	38.0	38.0	38.0	35.5	38.0
100	35.430204132154685	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	6.0
19	16.0
20	52.0
21	159.0
22	518.0
23	1313.0
24	3121.0
25	6197.0
26	11018.0
27	17636.0
28	26792.0
29	38790.0
30	53690.0
31	70967.0
32	93581.0
33	124924.0
34	184935.0
35	327466.0
36	910007.0
37	5346996.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.80849013748189	20.22697666325353	13.15629534119856	24.808237858066022
2	14.972349674343072	20.88856483054798	41.10366340319352	23.035422091915425
3	18.269975549261886	25.800024299730666	30.28272889023241	25.64727126077504
4	12.305137860236236	17.714950306032822	38.98316473127575	30.996747102455192
5	13.115696646782013	37.58118981507522	36.21552731920728	13.087586218935485
6	30.253331050197946	38.060836691537325	18.629498109209237	13.05633414905549
7	26.645591181490186	32.441344987054215	23.01667137468567	17.896392456769924
8	24.445168849186093	36.94199111217263	21.323955724616166	17.288884314025115
9	25.278134246175664	16.670127831268434	21.169526364445808	36.882211558110086
10-11	23.97419185731819	26.870556276804685	29.290021164594375	19.865230701282748
12-13	24.627423756131563	24.201444213069014	29.403539974788686	21.76759205601074
14-15	21.70245104483993	25.845645173781172	26.565043271946266	25.88686050943263
16-17	21.67635030790973	29.152902245397634	27.334862618549504	21.835884828143133
18-19	21.67975143896937	28.339179907641626	28.82247301157479	21.158595641814212
20-21	22.22927625235242	27.545103307021684	28.792530606409223	21.433089834216666
22-23	21.935639040410003	27.629263072609877	28.66340506040802	21.771692826572103
24-25	21.866607570616857	27.625057177714258	28.777035282433832	21.731299969235046
26-27	21.9328790022572	27.796116475931814	28.66973930982681	21.60126521198418
28-29	21.7147505307245	27.716320563282444	28.70237252598253	21.866556380010525
30-31	22.083732456275406	27.6837290163974	28.704797159548477	21.52774136777872
32-33	21.91051885147871	27.6805728993759	28.80143127610544	21.607476973039947
34-35	21.783444463627834	27.735106908672464	28.89382089953778	21.58762772816192
36-37	21.91160460386639	27.718617835005738	28.711577969328683	21.65819959179919
38-39	21.658571009714016	27.751331194039714	28.926676331595324	21.66342146465094
40-41	21.90579364851385	27.627135613010278	28.862232860038738	21.60483787843713
42-43	21.874089295035066	27.61264171815645	28.790868089718348	21.722400897090136
44-45	21.800030321848727	27.790031219563094	28.696381557179752	21.71355690140843
46-47	21.983796150320178	27.66854950835482	28.436279028930155	21.911375312394853
48-49	21.832660393728826	27.73824755580045	28.50501534621644	21.924076704254283
50-51	21.754747385392566	27.897510946659505	28.5471364105718	21.800605257376134
52-53	21.88014589675549	27.86500277160815	28.5796547393046	21.67519659233176
54-55	21.87245094011337	27.828267065018014	28.426758028867717	21.8725239660009
56-57	21.93856973191558	28.006936551636446	28.261169596808166	21.793324119639806
58-59	21.71503050134503	28.10525102049603	28.422384796452256	21.757333681706683
60-61	21.989931014176616	28.223441020808902	28.283809910865543	21.50281805414894
62-63	21.715457024702335	28.373917863602337	28.244714440992947	21.665910670702388
64-65	21.69600921288659	28.31686472904099	28.248463482291886	21.738662575780534
66-67	21.694306271565154	28.32467332612169	28.326483780591023	21.654536621722134
68-69	21.756383998701565	28.349288422718864	28.195730217175935	21.698597361403632
70-71	21.795732118549235	28.119770081470946	28.137001875976704	21.947495924003118
72-73	21.84957421933781	28.005980607398207	28.124990836166084	22.0194543370979
74-75	21.769688471379638	28.076808554332818	28.253173343333675	21.900329630953863
76-77	21.92539048776881	27.849108240532296	28.18213968680982	22.043361584889077
78-79	21.84433346663461	27.860527133938216	28.057129476843084	22.238009922584094
80-81	22.030588519312243	28.048417640738254	27.9945384711281	21.926455368821397
82-83	22.07925704102787	27.87063357399701	28.057686303219658	21.99242308175546
84-85	21.97369635148448	27.880573544180027	28.03349856386283	22.112231540472663
86-87	21.883206151886682	28.016675316096805	28.08785261016084	22.012265921855672
88-89	22.06688647695671	28.000448629997365	28.035005484428627	21.8976594086173
90-91	22.10900483236556	28.031858738285088	28.01591006716108	21.84322636218827
92-93	21.985777455472075	27.84727860581404	28.07831896782148	22.088624970892408
94-95	22.08734353462322	28.078159602131937	28.04923213166497	21.785264731579876
96-97	22.275498679058167	28.029817943845863	28.10793391528234	21.58674946181363
98-99	23.07266271828793	29.99980776770795	28.48932439779607	18.43820511620805
100	21.969449515943992	30.98113172947877	22.504831773298047	24.544586981279195
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	829.0
1	724.5
2	997.5
3	1607.5
4	2056.5
5	2702.5
6	3462.0
7	4428.5
8	5688.0
9	6557.0
10	6728.5
11	7003.0
12	7857.0
13	9365.5
14	12080.5
15	14896.0
16	16861.5
17	18033.5
18	18092.5
19	17528.5
20	17109.0
21	17483.0
22	19741.0
23	24270.5
24	31093.5
25	40851.0
26	53166.5
27	67252.5
28	83354.0
29	101189.0
30	119131.5
31	138053.5
32	157362.0
33	174366.0
34	190587.5
35	206326.5
36	221656.0
37	236097.0
38	248845.5
39	259534.5
40	270913.5
41	281843.5
42	288482.5
43	293943.5
44	298700.0
45	302829.0
46	312592.0
47	314828.0
48	306720.5
49	296895.0
50	282433.5
51	267344.5
52	251996.0
53	236209.5
54	219781.0
55	202083.5
56	183994.0
57	165545.5
58	145877.0
59	125208.5
60	103899.5
61	84130.0
62	66553.0
63	51025.5
64	37749.0
65	27362.5
66	20124.0
67	15316.5
68	11687.0
69	8662.5
70	6367.0
71	4580.5
72	3226.0
73	2220.0
74	1501.0
75	995.0
76	655.0
77	384.5
78	235.5
79	157.0
80	98.5
81	59.5
82	40.5
83	27.5
84	17.0
85	10.5
86	7.0
87	5.0
88	2.5
89	2.0
90	3.5
91	4.0
92	3.5
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	1.1083115469299977E-4
3	0.0
4	9.697726035637481E-5
5	0.003117126225740619
6	5.957174564748738E-4
7	8.312336601974984E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	8.246483654325977E-4
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	1.4143438367456853E-5
44-45	1.4215585754987981E-5
46-47	2.1449547553968672E-5
48-49	7.911712196652367E-5
50-51	3.977563360595553E-4
52-53	2.3980820575718458E-4
54-55	2.921026968819644E-4
56-57	3.597174324133156E-4
58-59	2.948975643820363E-4
60-61	6.664863204053008E-5
62-63	2.83078653180303E-4
64-65	2.248068515732995E-5
66-67	6.034844589173428E-5
68-69	4.787035795250121E-4
70-71	5.358618254255566E-5
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.001023294736171877
90-91	0.0
92-93	1.647405069312509E-5
94-95	4.9789081005590074E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	69.0
20-21	156.0
22-23	266.0
24-25	385.0
26-27	1327.0
28-29	4189.0
30-31	9675.0
32-33	13039.0
34-35	18773.0
36-37	29108.0
38-39	31775.0
40-41	31036.0
42-43	34236.0
44-45	40345.0
46-47	42158.0
48-49	38950.0
50-51	34272.0
52-53	32747.0
54-55	35662.0
56-57	37158.0
58-59	21077.0
60-61	40345.0
62-63	39016.0
64-65	42436.0
66-67	47199.0
68-69	48376.0
70-71	54567.0
72-73	40537.0
74-75	38933.0
76-77	40868.0
78-79	42139.0
80-81	41770.0
82-83	38912.0
84-85	38748.0
86-87	40668.0
88-89	43024.0
90-91	43401.0
92-93	43157.0
94-95	55229.0
96-97	235467.0
98-99	511854.0
100-101	5235138.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.58355167524388
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.90583797379695	79.61856157759394
2	6.661420787701296	11.668617835803605
3	1.4638815361228168	3.846358324963444
4	0.4674214919434783	1.6375373757500489
5	0.21364570146828588	0.9355924667370672
6	0.10979251964556784	0.5769611290759692
7	0.056816616969208054	0.3483340775834621
8	0.03362185442030086	0.23557771392303584
9	0.019937697020045556	0.15715928855164643
>10	0.06655803189726507	0.8723973598451615
>50	8.565739828632714E-4	0.05156966480210083
>100	1.8659263547840264E-4	0.037325042123605816
>500	2.2622396384422147E-5	0.014008143246862914
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3853894336624972E-5
2	0.0	4.156168300987492E-5	0.0	0.0	1.3853894336624972E-5
3	0.0	4.156168300987492E-5	0.0	1.3853894336624972E-5	1.3853894336624972E-5
4	0.0	5.541557734649989E-5	0.0	2.7707788673249945E-5	1.3853894336624972E-5
5	0.0	6.926947168312487E-5	0.0	2.7707788673249945E-5	1.3853894336624972E-5
6	1.3853894336624972E-5	6.926947168312487E-5	0.0	2.7707788673249945E-5	1.3853894336624972E-5
7	1.3853894336624972E-5	6.926947168312487E-5	0.0	2.7707788673249945E-5	1.3853894336624972E-5
8	1.3853894336624972E-5	9.697726035637481E-5	0.0	5.541557734649989E-5	1.3853894336624972E-5
9	1.3853894336624972E-5	1.8010062637612465E-4	0.0	6.926947168312487E-5	2.7707788673249945E-5
10-11	1.3853894336624972E-5	1.8010062637612465E-4	0.0	1.1083115469299978E-4	2.7707788673249945E-5
12-13	1.3853894336624972E-5	1.8010062637612465E-4	0.0	1.1775810186131228E-4	2.7707788673249945E-5
14-15	1.3853894336624972E-5	2.56297045227562E-4	0.0	1.3853894336624974E-4	2.7707788673249945E-5
16-17	2.7707788673249945E-5	2.632239923958745E-4	0.0	1.6624673203949968E-4	2.7707788673249945E-5
18-19	2.7707788673249945E-5	2.8400483390081193E-4	0.0	1.8010062637612465E-4	2.7707788673249945E-5
20-21	2.7707788673249945E-5	3.047856754057494E-4	0.0	2.008814678810621E-4	2.7707788673249945E-5
22-23	2.7707788673249945E-5	3.255665169106869E-4	0.0	2.4244315089093702E-4	3.4634735841562434E-5
24-25	2.7707788673249945E-5	3.809820942571868E-4	0.0	2.4937009805924953E-4	4.156168300987492E-5
26-27	2.7707788673249945E-5	3.8790904142549924E-4	0.0	3.255665169106869E-4	5.541557734649989E-5
28-29	2.7707788673249945E-5	3.948359885938117E-4	0.0	6.719138753263111E-4	5.541557734649989E-5
30-31	2.7707788673249945E-5	4.8488630178187403E-4	0.0	0.0016070517430484968	5.541557734649989E-5
32-33	2.7707788673249945E-5	4.8488630178187403E-4	0.0	0.0032418112747702437	6.926947168312487E-5
34-35	2.7707788673249945E-5	5.680096678016239E-4	0.0	0.005382237949778802	8.312336601974984E-5
36-37	2.7707788673249945E-5	6.442060866530613E-4	0.0	0.008797222903756858	8.312336601974984E-5
38-39	2.7707788673249945E-5	6.580599809896862E-4	0.0	0.019533991014641212	8.312336601974984E-5
40-41	2.7707788673249945E-5	7.41183347009436E-4	0.0	0.030270759125525563	8.312336601974984E-5
42-43	2.7707788673249945E-5	7.619641885143736E-4	0.0	0.03594392885637349	9.005031318806232E-5
44-45	2.7707788673249945E-5	8.589414488707483E-4	0.0	0.04385450252258635	9.697726035637481E-5
46-47	2.7707788673249945E-5	9.55918709227123E-4	0.0	0.05156419472091815	9.697726035637481E-5
48-49	2.7707788673249945E-5	9.697726035637481E-4	0.0	0.05911456713437875	9.697726035637481E-5
50-51	2.7707788673249945E-5	0.0011152384940983102	0.0	0.0672952917401558	9.697726035637481E-5
52-53	4.156168300987492E-5	0.0011360193356032477	0.0	0.09105472052746763	9.697726035637481E-5
54-55	4.8488630178187405E-5	0.0011429462827715602	0.0	0.11791742164618345	9.697726035637481E-5
56-57	5.541557734649989E-5	0.0011498732299398727	0.0	0.1496220588355497	9.697726035637481E-5
58-59	5.541557734649989E-5	0.0011498732299398727	0.0	0.293293870053519	1.039042075246873E-4
60-61	6.926947168312487E-5	0.001212215754454685	0.0	0.4202440308071819	1.1083115469299978E-4
62-63	6.926947168312487E-5	0.0012468504902962475	0.0	0.42507903993066404	1.1083115469299978E-4
64-65	6.926947168312487E-5	0.0013022660676427474	0.0	0.4293391124391762	1.1083115469299978E-4
66-67	6.926947168312487E-5	0.0013091930148110599	0.0	0.43332903400812417	1.1083115469299978E-4
68-69	6.926947168312487E-5	0.0013299738563159972	0.0	0.43728432084123064	1.1083115469299978E-4
70-71	6.926947168312487E-5	0.0013438277506526222	0.0	0.44067159800653544	1.1083115469299978E-4
72-73	8.312336601974984E-5	0.0013438277506526222	0.0	0.44332461877199913	1.2468504902962476E-4
74-75	8.312336601974984E-5	0.0013576816449892472	0.0	0.4454581184998394	1.2468504902962476E-4
76-77	8.312336601974984E-5	0.0014269511166723724	0.0	0.4477163032767092	1.2468504902962476E-4
78-79	8.312336601974984E-5	0.0014546589053456222	0.0	0.4492610124952429	1.2468504902962476E-4
80-81	8.312336601974984E-5	0.0014615858525139345	0.0	0.44982902216304455	1.2468504902962476E-4
82-83	8.312336601974984E-5	0.0014754397468505595	0.0	0.45024463899314326	1.2468504902962476E-4
84-85	8.312336601974984E-5	0.001496220588355497	0.0	0.4503693240421729	1.2468504902962476E-4
86-87	8.312336601974984E-5	0.001523928377028747	0.0	0.4504455204610244	1.2468504902962476E-4
88	8.312336601974984E-5	0.001565490060038622	0.0	0.4504455204610244	1.2468504902962476E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6920	0.0	47.63938	1
GTATCAA	11905	0.0	33.7095	1
TCAACGC	12810	0.0	30.298595	4
ATCAACG	13000	0.0	29.855772	3
CAACGCA	13310	0.0	29.160406	5
AACGCAG	13790	0.0	28.20856	6
TATCAAC	14675	0.0	26.92728	2
ACGCAGA	16560	0.0	23.437016	7
CGCAGAG	16875	0.0	23.051619	8
GTACATG	17035	0.0	22.500143	1
TACATGG	16800	0.0	21.925304	2
ACATGGG	16780	0.0	21.375147	3
CTTATAC	8175	0.0	20.162968	1
CATGGGG	12150	0.0	18.45036	4
GCAGAGT	21085	0.0	18.448946	9
GAGTACT	12475	0.0	18.11025	12-13
CAGAGTA	20430	0.0	17.405323	10-11
TATACAC	11595	0.0	17.24851	3
TATGCCG	5240	0.0	17.207565	42-43
AGAGTAC	18335	0.0	16.912897	10-11
>>END_MODULE