FastQCFastQC Report
Fri 27 May 2016
SRR2139749_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139749_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5998639
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65050.045.000881
GTATCAA109600.035.1434331
TCAACGC121150.030.9572834
ATCAACG123700.030.2123623
CAACGCA125050.030.0270045
AACGCAG129250.029.0838766
TATCAAC136250.027.5587312
ACGCAGA153850.024.404877
GTACATG170850.023.6523531
CGCAGAG159150.023.592148
TACATGG169950.023.0523682
ACATGGG169800.022.7875633
TATCTCG87800.022.21283794
ATCTCGT78500.021.9537294
CATGGGG124600.020.0667484
GCAGAGT193050.019.4949119
GAGTACT103750.019.07069812-13
AGAGTAC166250.017.84527410-11
CAGAGTA186850.017.65645410-11
GAGTACA119250.017.2387261
Produced by FastQC (version 0.11.5)